Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.     

上海动物园非洲企鹅种群线粒体DNA序列分析及遗传管理建议

非洲企鹅(Spheniscus demersus)是一种濒危海鸟,也是动物园展出的重要物种。上海动物园从日本和荷兰引进非洲企鹅,种群不断扩大,并成为国内种群发展的基础。但对其遗传多样性和结构了解很少,无法开展有效的遗传管理。本研究分析了上海动物园39只非洲企鹅的mtDNA D-loop区430 bp序列,并与已发表的日本种群的数据进行比较。结果表明,该片段存在15个变异位点,占分析位点的3.5%;共定义了10个单倍型,其遗传距离为0.002~0.019。日本种群包含A、B两个mtDNA D-loop单倍型世系,上海种群的10个单倍型均属于A世系,且与日本种群有5个共享单倍型,其遗传多样性水平略低于日本种群。上海种群可以进一步分为3个小的世系,但置信度偏低(54%~87%)。上述结果提示,上海种群相对于日本种群的A世系而言有较好涵盖度,但仍有必要进一步引进建群个体,尤其是含有B世系单倍型的个体。     

舟山长白岛民俗文化探析

以长白岛为例,通过对长白岛民俗文化的现状分析,发现其存在的问题,由此提出保护和发展长白岛民俗文化的建议和措施,希望对长白岛的文化、经济、旅游业发展具有一定的借鉴意义.     

Functional porous carbons from waste cotton fabrics for dyeing wastewater purification

A new functional porous carbons (PC-WF) is prepared by activation-pyrolysis method use waste cotton fabrics (WF) as an abundant, cheap and available precursor for removal of Brilliant Crocein (BC-GR) and Cationic Red 2GL (CR-GL) from aqueous phase. The PC-WF was characterized by BET, FTIR, SEM, and XRD techniques, the surface area, total pore volume, average pore diameter was found as 1463.5 m² g^-1, 0.783 cm³ g^-1 and 2.14 nm, respectively. The influences on BC-GR and CR-GL adsorption of various experimental factors such as initial concentration and temperature were investigated. Adsorption kinetics was found to be best represented by the pseudo-second order model. The adsorption capacity was 319.8 mg g^-1 for BC-GR and 842.5 mg g^-1 for CR-GL at 30 °C, respectively. The results indicate that for waste cotton fabrics in particular, the practical application of this process to the production of porous carbon would be possible.     

杀虫植物鱼藤根色谱指纹图谱研究

为构建杀虫植物鱼藤根及其产品的质量标准和控制体系及建立鱼藤根的高效指纹图谱,采用高效液相色谱法(HPLC)对鱼藤根的主要特征成分进行定性分析,研究丰顺鱼藤根中染料木素(4′,5,7-三羟基异黄酮)、鱼藤酮、反查尔酮、灰毛素、脱氢灰毛素等5种化学成分的变化规律。结果表明:当λ为240nm时色谱图中的峰为34个;而当λ为254nm时,色谱图中的峰为37个。该研究为建立杀虫植物鱼藤根原产地识别技术体系及鱼藤酮产品的质量技术标准,对植物源农药的登记及质量体系、产品标准的建立具有重要的指导和现实意义。     

搭建农民合作经济组织服务平台 探索棉花轻简栽培技术推广途径

<正>近年来,农民迫切需要更多的致富、增效技术,对农业新技术应用热情高涨。中国农业科学院棉花研究所研发的棉花基质育苗轻简栽培技术,在江苏省射阳县多年试验、示范,获得成功。该项技术同时被农民合作经济组织所接受并推广应用。射阳县原银棉花专业合作社成功地走出了一条"合作社+基地+农户"农业新技术推广的路子,高产典型多,棉农效益高;形成了"代育苗"和"代移栽"服务模式,加快了先进科学技术转化现实生产力的速     

连云港口岸国际航行船舶截获植物有害生物情况分析与建议

为强化对国际航行船舶的检疫监管,降低植物有害生物传入风险,本文对"十二五"期间连云港口岸国际航行船舶截获植物有害生物的情况进行了统计,掌握了截获有害生物的种类,分析了影响截获的主要因素,并提出相应的检疫对策。     

我国与美国材料燃烧技术标准比较研究

针对我国与美国的建筑材料与制品、家具及纺织织物主要燃烧技术标准体系的异同进行了比较与分析.系统比较了相关产品的技术和标准,对打破国外技术性贸易壁垒并建立完善我国的法规和标准具有十分重要的意义.