Using synergists to detect multiple insecticide resistance in field populations of rice stem borer

The rice stem borer, Chilo suppressalis (Walker), an important insect pest of rice in China, has developed resistances to several classes of insecticides in field. In order to investigate multiple resistance mechanisms, synergistic tests were conducted with the Ruian (RA) population and Lianyungang (LYG) population, two representative populations to different insecticides. Results showed that diethyl maleate (DEM), S,S,S-tributyl phosphorotrithioate (DEF) and piperonyl butoxide (PBO), had no significant synergistic or inhibitory effect on the high level of resistance to monosultap (313.4-fold) and moderate level to chlorpyrifos (36.9-fold) in Ruian field population from the year 2011 (RA11). DEF significantly synergized the activity of triazophos in RA11 population (536.8-fold), with synergism ratio of 1.92. DEF and PBO significantly suppressed 43.3% and 40.4% of resistance to fipronil in RA11 population (48.4-fold), respectively, with the synergistic ratios of 1.76 and 1.69. When pretreated with PBO, the activity of deltamethrin against RA11 population were significantly synergized, with synergism ratio of 9.57, and with reducing resistance levels from 152.5- to 15.9-fold. The results of this study indicated that resistance to several classes of insecticide among the field populations of C. suppressalis might be provided by the combination of the multiple resistance mechanisms. Metabolic resistance mechanism might be the major reason for the evolution for resistance to deltamethrin and fipronil, while resistance to monosultap, triazophos and chlorpyrifos is not associated with metabolic resistance.     

上海外高桥口岸从进境羊毛中截获的杂草种类

上海外高桥口岸是原毛进境的重要口岸,外高桥检验检疫局在进境羊毛检验检疫中发现进境羊毛携带有大量杂草籽。本文对外高桥局2011年1～9月份进境羊毛中截获的杂草进行了统计与分析,共截获杂草10112批次,73种;其中检疫性杂草4种,435批次。并针对外来入侵杂草管理提出对策和建议。     

PCR方法检测美人蕉黄斑驳病毒

美人蕉黄斑驳病毒病是危害美人蕉的重要病害之一,我国目前尚无该病发生的报道.本文以健康和感病植株为材料,进行总DNA的提取和PCR扩增,从感病植株中扩增出与目的片段大小相符的产物,而健康植株中则未扩增出.PCR扩增产物经克隆、测序后证实,与已报道的美人蕉黄斑驳病毒(Cannayellow mottle virus,CaYMV)序列同源性高达97%～99%.经优化反应条件,建立了稳定的美人蕉黄斑驳病毒PCR检测方法.     

中国口岸首次截获外来有害杂草——胀果苋

胀果苋(Amaranthus deflexus L.)为一年生或短期多年生植物,广泛分布于热带、亚热带与暖温带地区,但在中国尚未分布。由于胀果苋为恶性杂草,对农田、果园、路旁、园林绿化等危害严重。福建出入境检验检疫局首次截获胀果苋,系我国口岸首次截获该种外来杂草。本文根据截获的胀果苋种子及栽种植株,详细对其形态特征进行了描述;并根据资料,介绍了胀果苋的分类地位、地理分布、生物学与危害性,为口岸胀果苋的鉴定识别提供参考。     

白菜病程相关蛋白1基因cDNA全长的克隆与分析

 以水杨酸处理后的‘苏州青’白菜为材料, 通过RT2PCR和RACE技术, 获得了白菜病程相关蛋白PR-1基因的cDNA全长序列。该基因与甘蓝型油菜PR-1基因氨基酸序列的同源性为91% , 与拟南芥的同源性为78% , 与其它植物的同源性为57%～60%。Southern杂交表明该基因在白菜基因组中的拷贝数至少为2个。半定量RT2PCR分析表明, 2 mmol/L的水杨酸处理后12 h, 该基因的表达量达到高峰。     

不结球白菜亮白叶再生体系的优化

摘 要：本文以不结球白菜品种亮白叶为实验材料,研究不同外植体类型和不同生长调节剂配比等因素对其不定芽分化频率的影响。结果表明,对不结球白菜品种中再生比较困难的品种亮白叶来说,以下胚轴、子叶及子叶柄为外植体的诱导频率存在明显差异。以生长5-6天无菌苗的子叶柄为外植体,在培养基为MS+6-BA（5 mg/L）+NAA（0.1 mg/L）+ AgNO3（4mg/L）的培养条件下,亮白叶分化不定芽的再生频率最高,可达59.5%;这为将来该不结球白菜转化体系的建立奠定了坚实基础。     

上海市犬只狂犬病认定免疫点主要管理做法分析

强化犬只狂犬病免疫工作是从源头控制狂犬病的最有效措施.近年来,上海市按照《上海市养犬管理条例》的要求,通过严把免疫资质准入、规范内部管理、落实疫情报告责任、推进免疫质量考核等举措,使犬只狂犬病认定免疫点的布局日趋合理,文明服务更加规范,这已成为上海市民犬只狂犬病免疫的主渠道,免疫数量和质量逐年提升,构筑了良好的免疫屏障...     

浅析基层农技推广的现状与改进的策略

农业方面科学技术的渗透是国民发展的基础。要实现现代化农业的建设、城乡统筹的发展、农村全面小康化的目标基层农机推广起着至关重要的作用。