全文获取类型
收费全文 | 150篇 |
免费 | 12篇 |
专业分类
林业 | 3篇 |
农学 | 2篇 |
11篇 | |
综合类 | 9篇 |
农作物 | 12篇 |
水产渔业 | 17篇 |
畜牧兽医 | 102篇 |
园艺 | 5篇 |
植物保护 | 1篇 |
出版年
2023年 | 2篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 3篇 |
2015年 | 1篇 |
2014年 | 5篇 |
2013年 | 8篇 |
2012年 | 12篇 |
2011年 | 12篇 |
2010年 | 7篇 |
2009年 | 6篇 |
2008年 | 17篇 |
2007年 | 8篇 |
2006年 | 13篇 |
2005年 | 5篇 |
2004年 | 9篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1940年 | 1篇 |
排序方式: 共有162条查询结果,搜索用时 15 毫秒
61.
Oremland RS Kulp TR Blum JS Hoeft SE Baesman S Miller LG Stolz JF 《Science (New York, N.Y.)》2005,308(5726):1305-1308
Searles Lake is a salt-saturated, alkaline brine unusually rich in the toxic element arsenic. Arsenic speciation changed from arsenate [As(V)] to arsenite [As(III)] with sediment depth. Incubated anoxic sediment slurries displayed dissimilatory As(V)-reductase activity that was markedly stimulated by H2 or sulfide, whereas aerobic slurries had rapid As(III)-oxidase activity. An anaerobic, extremely haloalkaliphilic bacterium was isolated from the sediment that grew via As(V) respiration, using either lactate or sulfide as its electron donor. Hence, a full biogeochemical cycle of arsenic occurs in Searles Lake, driven in part by inorganic electron donors. 相似文献
62.
Shelley H. Jansky Georgia L. Davis Stanley J. Peloquin 《American Journal of Potato Research》2004,81(5):335-339
Wild diploidSolanum species contain valuable genes for potato improvement, but do not tuberize under the long-day conditions of temperate growing regions. Crosses to haploids (2n=2x=24) of the cultivated potato (Solanum tuberosum) produce hybrids that often tuberize under long days. The objectives of this work are (1) to document high levels of tuberization in haploid-wild species hybrid populations, (2) to evaluate the genetic basis of tuberization in haploid-wild species hybrids grown under long-day conditions, and (3) to propose a genetic model for tuberization in haploid-wild species hybrids. Tuberization under long-day conditions was evaluated in 154 haploid-wild species hybrid families. An average of 68% of plants in these families tuberized. Two major genes exhibiting duplicate dominant epistasis appear to regulate tuberization under long-day conditions. Based on this model, the haploid parent genotypes are A-B-, aaB-, or A-bb, while the wild species are aabb. Future studies are planned to identify the genetic components of tuberization. 相似文献
63.
64.
65.
Starch Characteristics of Modern and Heirloom Potato Cultivars 总被引:2,自引:0,他引:2
Diego Fajardo Kathleen G. Haynes Shelley Jansky 《American Journal of Potato Research》2013,90(5):460-469
In a number of ways, modern potato breeding efforts differ from those that created heirloom cultivars. As a result of the rapid expansion of the potato processing industry in the mid twentieth century, potato breeders shifted their focus from fresh market varieties to those intended for the fry and chip markets. Selection for higher dry matter content and lower reducing sugar levels in tubers was successful. This study sought to determine whether modern processing cultivars differ from heirloom cultivars for two major characteristics of tuber starch, amylose content and starch granule morphology. Since breeding efforts for processing potatoes have focused on the maintenance of tuber quality during storage, these parameters were measured in both fresh and stored tubers. Twenty cultivars selected to span the range of cultivar release dates in the U.S. were grown at Hancock, WI in 2009 and 2010 and evaluated for tuber amylose content and measurements of starch granule surface area, length, width, length to width ratio, and smoothness. These traits were found to be stable across years and during storage. Significant differences in the amylose content and starch granule morphology exist among cultivars, but there is no clear distinction between modern and heirloom cultivars. Genetic variation for amylose content and starch granule morphology was found within the set of clones sampled for this study. However, germplasm with higher amylose content will need to be identified and utilized in breeding if the nutritional benefits associated with high fiber and low glycemic index are to be realized. 相似文献
66.
Wild diploid Solanum species with an endosperm balance number (EBN) of one are a rich source of disease resistance, pest resistance, and tuber
quality traits. They are not sexually compatible with 4× 4EBN or 2× 2EBN forms of the cultivated potato. In this study, the
wild 2× 2EBN Mexican species S. verrucosum (ver) was used as a bridge species to access 2× 1EBN germplasm. Hybrids (V1) were created between ver as a female parent
and the 1EBN species S. cardiophyllum, S. chancayense, S. commersonii, and S. trifidum. Most of the V1 hybrids were successfully crossed as females to cultivated 2× 2EBN clones. However, due to stylar barriers,
reciprocal crosses were unsuccessful. Additional studies are underway to determine the effectiveness of ver as a bridge to
introgress specific traits from 2× 1EBN germplasm into the cultivated potato. 相似文献
67.
Preziosi DE Morris DO Johnston MS Rosenthal KL O'Shea K Rankin SC 《Journal of the American Veterinary Medical Association》2006,228(2):216-221
OBJECTIVE: To ascertain whether Malassezia organisms can be detected via cytologic examination and fungal culture of samples from the skin surface of psittacine birds and determine whether the number of those organisms differs between unaffected psittacines and those that have chronic feather-destructive behavior or differs by body region. DESIGN: Prospective study. ANIMALS: 50 unaffected psittacines and 53 psittacines that had feather-destructive behavior. PROCEDURE: Samples were collected by use of acetate tape strips from the skin of the head, neck, proventer, propatagium, inguinal region, and preen gland area of each bird; 0.5-cm(2) sample areas were examined microscopically for yeast, and samples were also incubated on Sabouraud dextrose agar. Polymerase chain reaction assays specific for Malassezia spp, saprophytic fungi, and Candida albicans were performed on DNA prepared from cultured colonies; nested PCR evaluation for Malassezia pachydermatis was then performed. RESULTS: Microscopically, 63 of 618 (10%) tape-strip samples contained yeast. Thirty cultured colonies were assessed via PCR assays, and all yielded negative results for Malassezia spp; C albicans was identified in 2 colony samples. The numbers of yeast identified microscopically in psittacines with feather-destructive behavior and in unaffected birds did not differ significantly, and numbers did not differ by body region. CONCLUSIONS AND CLINICAL RELEVANCE: Yeast were identified infrequently via cytologic examination of samples from the skin surface of unaffected psittacine birds or those that had chronic feather-destructive behavior. If yeast are identified on the skin of birds with feather-destructive behaviors, fungal culture of skin samples should be performed to identify the organism. 相似文献
68.
- 1. The Hawkesbury‐Nepean River provides potable water for 5 million people living in the Sydney basin, and water for agricultural and horticultural production that meets most of Sydney's daily needs for fresh food. Anecdotal evidence indicated that numbers of freshwater mussels have seriously declined in much of the river over recent decades.
- 2. A field survey revealed the presence of populations of three species of mussels, Hyridella depressa, Hyridella australis and Velesunio ambiguus in the river. Higher density mussel populations were most common in catchment areas with little human modification to the channel bed or adjacent vegetation communities.
- 3. Levels of disturbance of riparian vegetation and, to a lesser degree, land use, were identified as being strongly associated with the absence of mussels from some reaches.
- 4. Catchment geomorphology was also shown to be relevant to the abundance, population structure and suite of mussel species present in different geomorphic reaches of the river. Absence of mussels was noted from areas where they had been recorded in previous studies. These results demonstrate that mussel species are under threat in the catchment.
- 5. As filter feeders with the ability to remove excess nutrients and bioaccumulate toxic substances, freshwater mussels play an important role in natural remediation processes in freshwater systems.
- 6. The decline in mussel populations in the Hawkesbury‐Nepean River highlights concerns for the overall health of the river system, and supports the need to identify the subsidiary impacts of physical habitat modification in developing both riverine and riparian management strategies.
69.
Daniel O. Morris Raymond C. Boston Kathleen O’Shea Shelley C. Rankin 《Veterinary dermatology》2010,21(4):400-407
It has been shown that people and pets can harbour identical strains of meticillin‐resistant (MR) staphylococci when they share an environment. Veterinary dermatology practitioners are a professional group with a high incidence of exposure to animals infected by Staphylococcus spp. The objective of this study was to assess the prevalence of carriage of MR Staphylococcus aureus (MRSA), MR S. pseudintermedius (MRSP) and MR S. schleiferi (MRSS) by veterinary dermatology practice staff and their personal pets. A swab technique and selective media were used to screen 171 veterinary dermatology practice staff and their respective pets (258 dogs and 160 cats). Samples were shipped by over‐night carrier. Human subjects completed a 22‐question survey of demographic and epidemiologic data relevant to staphylococcal transmission. The 171 human‐source samples yielded six MRSA (3.5%), nine MRSP (5.3%) and four MRSS (2.3%) isolates, while 418 animal‐source samples yielded eight MRSA (1.9%) 21 MRSP (5%), and two MRSS (0.5%) isolates. Concordant strains (genetically identical by pulsed‐field gel electrophoresis) were isolated from human subjects and their respective pets in four of 171 (2.9%) households: MRSA from one person/two pets and MRSP from three people/three pets. In seven additional households (4.1%), concordant strains were isolated from only the pets: MRSA in two households and MRSP in five households. There were no demographic or epidemiologic factors statistically associated with either human or animal carriage of MR staphylococci, or with concordant carriage by person–pet or pet–pet pairs. Lack of statistical associations may reflect an underpowered study. 相似文献
70.