60Co辐照对澳洲坚果种子萌发与幼苗形态的影响

澳洲坚果品种‘云澳57号’、‘云澳51号’、‘云澳41号’、‘云澳58号’的种子以剂量率为10 Gy/h的60Co-γ射线进行辐照处理,剂量分别为40 Gy、120 Gy和200 Gy。结果表明：60Co-γ射线辐照对澳洲坚果种子萌发有抑制作用,且不同品种对辐照剂量的敏感性不同;较低剂量（40Gy）的60Co-γ射线辐照会显著提高品种‘云澳41号’的萌发率,而品种‘云澳57号’则对所有剂量的辐照均不敏感;随着60Co-γ射线辐照剂量的增加,4个澳洲坚果品种的幼苗真叶长度、宽度和叶面积呈显著降低趋势;且60Co-γ射线辐照会使幼苗茎干变短、茎围增粗,其变化幅度因品种而异。     

砂梨上苹果茎痘病毒的调查分析及RT-PCR与TC-RT-PCR检测研究

试验通过西方烟(Nicotianaoccidentalis‘37B’)和杂种(Pyroniaveitchii)、A20接种鉴定及PAS-ELISA检测,对保存于国家砂梨种质资源圃的38份砂梨样品进行了苹果茎痘病毒(Applestempittingvirus,ASPV)带毒率的调查分折。结果表明,生物学鉴定与血清学检测结果一致,ASPV带毒率均为26.3%。应用以dsRNA为模板RT-PCR检测该病毒,在杂种和A20上显症的6个样品均获得316bp目标片段。应用TC-RT-PCR检测显症的西方烟,也获得了预期的目标片段。     

应用SSCP技术分析我国柑橘衰退病毒的混合感染

以随机采样的方式,从湖北、湖南和江西3省10县的柑橘产区收集242份温州蜜柑(CitrusunshiuMarc.)、橘(C.reticulataBlanco)、甜橙(C.sinensisOsbeck)和柚(C.grandisOsbeck)样品。对PAS-ELISA法初步检测呈阳性的样品进行柑橘衰退病毒(Citrustristezavirus,CTV)CP基因(coatingproteingene,CPgene)的RT-PCR扩增,结果来自14个样品采集地的120份样品感染了CTV,单个采样点的感染率为20.0%～71.4%,而总感染率达49.6%,表明CTV在这些地区广泛发生。单链构象多态性(single-strandconformationpolymorphism,SSCP)分析显示,120份CTV的CP基因RT-PCR的扩增产物共产生120种SSCP带型,而且存在广泛的混合感染。     

Construction of siArid2 recombinant adenovirus and its effect on proliferation of hepatocarcinoma cells

AIM: To observe the effects of Arid2gene on cell proliferation and cell cycle by interference of endogenous Arid2 expression in hepatoma cells. METHODS: Three pairs of shRNA targeting Arid2gene were cloned into a shuttle vector to construct recombinant adenovirus plasmids. HEK293 cells were transfected with the recombinant adenovirus plasmids. After several rounds of the package and amplification, the high-titer adenoviruses AdsiArid2-1~3 were obtained. To verify the inhibitory effects of AdsiArid2 adenoviruses, Western blotting was used to detect the endogenous Arid2 protien expression in SMMC-7721 cells. Cell growth and cell cycle analysis were carried out by MTS assay and flow cytometry. RESULTS: High- titer recombinant adenovirus of siArid2 were successfully obtained, and named AdsiArid2-1~3, among which the AdsiArid2-3 had the best inhibitory effects. MTS assay showed that the absorbance values at 490 nm were increased at 72 h and 96 h after transduction compared with the mock and Adsicontrol groups. These data indicated that knockdown of Arid2 promoted the proliferation rate of SMMC-7721 cells(P<0.05). Moreover, the flow cytometry analysis revealed that the G1-phase distribution at 72 h in AdsiArid2 group was lower than that in mock group and Adsicontrol group. In contrast, the S-phase distribution in AdsiArid2 group was much higher than that in mock group and Adsicontrol group. CONCLUSION: The recombinant plasmids and recombinant adenovirus were successfully constructed. shRNA-mediated knockdown of Arid2 promotes the proliferation and the transition from G1 phase to S phase of hepatoma cells.     

蜜蜂与壁蜂对大樱桃授粉效果对比研究

为了解蜜蜂和壁蜂两种授粉方式对大樱桃坐果率、产量及果实品质的影响,本试验开展了蜜蜂与壁蜂的大樱桃授粉效果对比研究.结果表明,蜜蜂授粉区效果最好,坐果率为62.8％,每667 m2产量为1151.5 kg;壁蜂授粉区次之,坐果率为45.3％,每667 m2产量为1147.5 kg;常规授粉区坐果率为25.3％,每667 ...     

柑橘衰退病毒侵染对寄主植物同工酶及酸性病程相关蛋白表达的影响

以柑橘衰退病毒(Citrus tristeza virus,CTV)不同致病力株系接种寄主植物,比较分析了寄主植物体内过氧化物酶(peroxidase,POD)和多酚氧化酶(polyphenol oxidase,PPO)活性变化特点。结果表明,该病毒侵染对这2种酶活性的影响因寄主品种和CTV株系而异,CTV强毒和弱毒株系侵染均导致墨西哥莱檬植株中POD活性明显降低;在甜橙上,CTV强毒株系接种植株中POD活性则显著性提高,而弱毒株系接种对POD的活性无明显影响;在对该病毒具有较高抗性的枳壳中,接种CTV不同株系对POD活性均无明显影响。而在接种强毒株系N25的墨西哥莱檬中PPO活性有所增强,在枳壳上则表现为接种植株的PPO活性降低,在甜橙上PPO活性无明显的变化。POD、PPO和酯酶(esterase,EST)同工酶酶谱分析的结果显示,CTV弱毒株系接种枳壳后诱导产生了2条新的EST同工酶谱带,而在其他CTV接种植株上均未诱导表达新的POD、PPO及EST酶谱。酸性病程相关蛋白分析结果显示,CTV强毒株系在甜橙上诱导产生有6条明显的酸性病程相关蛋白条带。     

中国柑橘黄龙病菌16S rDNA序列研究

 运用PCR技术对来自中国7省区不同寄主上的黄龙病菌16S rDNA基因区进行了PCR-RFLP-SSCP分析, 采用3种限制性内切酶进行单酶切、双酶切及三酶切反应,对酶切产物进行了单链构象多态性（SSCP）分析,结果表明来自7省区不同寄主上9个黄龙病病菌分离物的16S rDNA无可见变异;同时对9个代表性的分离物16S rDNA进行了克隆测序,序列多重比对结果与PCR-RFLP-SSCP一致,从而在分子水平上证明了中国柑橘黄龙病病菌的16S rDNA序列高度保守, 在不同的地域、寄主内没有发现分子变异,为进一步研究柑橘黄龙病菌系统进化奠定了基础。     

The spleen morphophysiology of fruit bats

Spleen is one of the important lymphoid organs with wide variations of morphological and physiological functions according to species. Morphology and function of the spleen in bats, which are hosts to several viral strains without exhibiting clinical symptoms, remain to be fully elucidated. This study aims to examine the spleen morphology of fruit bats associated with their physiological functions. Spleen histological observations were performed in three fruit bats species: Cynopterus titthaecheilus (n = 9), Rousettus leschenaultii (n = 3) and Pteropus vampyrus (n = 3). The spleens of these fruit bats were surrounded by a thin capsule. Red pulp consisted of splenic cord and wide vascular space filled with blood. Ellipsoids in all three studied species were found numerously and adjacent to one another forming macrophages aggregates. White pulp consisted of periarteriolar lymphoid sheaths (PALS), lymphoid follicles and marginal zone. The lymphoid follicle contained a germinal centre and a tingible body macrophage that might reflect an active immune system. The marginal zone was prominent and well developed. This study reports some differences in spleen structure of fruit bats compared to other bat species previously reported and discusses possible physiological implications of the spleen based on its morphology.     

西双版纳州4种臂形草属牧草引种试验

2005、2006和2007年,分别在景洪市嘎洒镇选取海拔为550、560和846 m的3个试验点,种植新引进的杂交臂形草1号（Brachiaria brizantha Mulato 1)、杂交臂形草2号（B.brizantha Mulato 2)、热研3号俯仰臂形草（B.decumbens cv.Reyan No.3）和热研6号珊状臂形草（B.brizantha cv.Reyan No.6）,进行比较试验。结果表明,4种牧草均有分蘖数多、产量高、生产性能稳定,营养价值高、适口性好、容易刈割,抗病力强、对土壤和气候条件的要求不高、适应性强、容易种植、便于推广等优点。热研3号俯仰臂形草、热研6号珊状臂形草根系发达,利于控制水土流失,也有利于草地防火,一次种植可长期受益,建议在西双版纳州、德宏州和海南省推广种植;杂交臂形草1号和杂交臂形草2号出苗快、孕穗期长、抽穗期晚、茎叶比小、适口性更好、消化利用效率更高,建议重点推广应用。