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71.
Kim B Min K Choi C Cho WS Cheon DS Kwon D Kim J Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):341-342
The in vitro susceptibilities of 76 isolates of Actinobacillus pleuropneumoniae collected from pigs with pleuropneumonia were tested with 12 commonly used antimicrobial drugs by an agar dilution minimal inhibitory concentration procedure according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Field isolates had low MICs for ceftiofur, danofloxacin and penicillin. No correlation of antimicrobial resistance was related to serotype. 相似文献
72.
cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug 总被引:1,自引:0,他引:1
Lee GJ Lee WS Jeon KS Um CH Kim YS Kim SJ Lee CH Yoon HK Hwang SY Park JS Hwang JW Kang KS Lee YS Kim MS Chon KJ Yeo CD Kang JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1339-1345
Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype. 相似文献
73.
One of the great puzzles of visual perception is how an image that is in perpetual flux can still be seen by the observer as the same object. In an informative Perspective, Seung and Lee explain the mathematical intricacies of two new algorithms for modeling the variability of perceptual stimuli and other types of high-dimensional data (Tenenbaum et al., and Roweis and Saul). 相似文献
74.
Moo Sung Lee Min Gyu Ha Hyun Jin Ko Kap Seung Yang Wan Jin Lee Min Park 《Fibers and Polymers》2000,1(1):32-36
An alternative strategy to reduce the percolation threshold of carbon black (CB) in polymer blends was investigated using
random copolymer ternary blends of polystyrene (PS), poly(methyl methacrylate) (PMMA), and a styrene-methyl methacrylate random
copolymer (SMMA). The target morphology was to selectively locate CB particles in the encapsulating layer of SMMA during melt
mixing. The CB used in this study is BP-2000 from Cabot and has a strong selective affinity to PS. Even when the CB was premixed
with SMMA, it moves to the PS phase during the melt mixing. However, we also observed the CB particles located at the interface
between SMMA and PS phases. Through this study it is found that the interaction between polymers and CB particles is critical
for selectively localizing CB particles in multi-component polymer blends. Although appropriate processing condition may retard
the movement of CB particles to the polymer phase with affinity, it cannot prevent it completely and locate them to the SMMA
phase, which is not thermodynamically favored. To locate CB particles in an encapsulating layer of ternary polymer blends,
first of all, polymers forming it should have selective affinity to CB. 相似文献
75.
Kap Seung Yang Young Ok Choi Yong Min Kim Sang Hee Park Cheol Min Yang Yong Joong Kim Soon Young Soh 《Fibers and Polymers》2000,1(2):97-102
Pitch precursors were synthesized from coal tar(CT) and pyrolysis fuel oil(PFO, petroleum residue oil) at relatively low temperature
of 250°C, in the presence of borontrifluoride/diethyletherate complex(BFDE) as a catalyst and nitrobenzene(NB) as a co-catalyst.
The softening point, nitrogen content and carbon yield increased with an increase of concentration of NB. The pitch precursors
with good spinnability were prepared by removing the volatile components through N2 blowing. The precursor pitches were spun through a circular nozzle, stabilized at 310°C and finally carbonized at 1000°C.
The optically anisotropic structure formed at the absence of NB was changed into isotropic structure, showing a decrease in
size of the flow domain. The hollow carbon fiber could be prepared in the process of stabilization. The results proposed that
the morphology of carbon materials could be controlled by changing the concentration of catalyst and/or co-catalyst and/or
stabilization condition that affect on the mobility of molecules during carbonization. 相似文献
76.
The present study was undertaken to compare the effect of salinomycin and Bacillus subtilis on growth performance, serum antibody levels against Clostridium spp. and Eimeria spp., and cytokine mRNA expression levels in broiler chickens raised in the used litter. Broiler chickens fed a diet containing salinomycin showed lower (P < 0.05) body weights compared with the control diet-fed counterparts. Serum nitric oxide levels were significantly (P < 0.05) elevated in chickens fed the B. subtilis-enriched diet compared with those on either the salinomycin-fed or control diet-fed chickens. None of the dietary treatments affected (P > 0.05) serum antibody levels against Clostridium perfringens toxins. Both salinomycin and B. subtilis significantly lowered (P < 0.05) the serum levels of Eimeria-specific antibodies compared with the control group. Salinomycin, but not B. subtilis, significantly modulated (P < 0.05) the expression of cytokines encoding interferon-γ (IFN-γ), interleukin10 (IL-10) and tumor necrosis factor superfamily 15 (TNFSF15) compared with the control group. In conclusion, dietary salinomycin and B. subtilis affected serum anticoccidial antibody and intestinal cytokine expression, but failed to improve growth performance in broiler chickens. Further study is warranted to investigate the mode of action of salinomycin on host immune response and growth performance in broiler chickens. 相似文献
77.
Chang‐Hoon Kwon Seung‐ Jae Han Sung‐Jae Kim Byung‐Chul Park Insurk Jang Jeong‐Hee Han 《Animal Science Journal》2014,85(8):805-813
This study was aimed to evaluate the effect of dietary supplementation of lipid‐encapsulated (coated) zinc oxide ZnO on post‐weaning diarrhea (colibacillosis) in weaned piglets challenged with enterotoxigenic Escherichia coli (ETEC). Thirty‐two 35‐day‐old weaned piglets were orally challenged with 3 × 1010 colony forming units of ETEC K88 while eight piglets received no challenge (control). Each eight challenged piglets received a diet containing 100 ppm ZnO (low ZnO), 2500 ppm ZnO (high ZnO) or 100 ppm of lipid (10%)‐coated ZnO (coated ZnO) for 7 days; control pigs received the low ZnO diet. Daily gain, goblet cell density in the villi of the duodenum, jejunum and ileum, and villus height in the jejunum and ileum, which decreased due to the challenge, were equally greater in the coated ZnO and high ZnO groups versus low ZnO group. Fecal consistency score, serum interleukin‐8 concentration, subjective score of fecal E. coli shedding, and digesta pH in the stomach, jejunum and ileum, which increased due to the challenge, were equally low in the coated ZnO and high ZnO groups versus low ZnO. Results suggest that a low level of coated ZnO might well substitute for a pharmacological level of native ZnO in dietary supplementation to alleviate colibacillosis of weaned piglets. 相似文献
78.
Ryu JH Kim SH Lee HY Bai JY Nam YD Bae JW Lee DG Shin SC Ha EM Lee WJ 《Science (New York, N.Y.)》2008,319(5864):777-782
Although commensalism with gut microbiota exists in all metazoans, the host factors that maintain this homeostatic relationship remain largely unknown. We show that the intestinal homeobox gene Caudal regulates the commensal-gut mutualism by repressing nuclear factor kappa B-dependent antimicrobial peptide genes. Inhibition of Caudal expression in flies via RNA interference led to overexpression of antimicrobial peptides, which in turn altered the commensal population within the intestine. In particular, the dominance of one gut microbe, Gluconobacter sp. strain EW707, eventually led to gut cell apoptosis and host mortality. However, restoration of a healthy microbiota community and normal host survival in the Caudal-RNAi flies was achieved by reintroduction of the Caudal gene. These results reveal that a specific genetic deficiency within a host can profoundly influence the gut commensal microbial community and host physiology. 相似文献
79.
Foroughi J Spinks GM Wallace GG Oh J Kozlov ME Fang S Mirfakhrai T Madden JD Shin MK Kim SJ Baughman RH 《Science (New York, N.Y.)》2011,334(6055):494-497
Rotary motors of conventional design can be rather complex and are therefore difficult to miniaturize; previous carbon nanotube artificial muscles provide contraction and bending, but not rotation. We show that an electrolyte-filled twist-spun carbon nanotube yarn, much thinner than a human hair, functions as a torsional artificial muscle in a simple three-electrode electrochemical system, providing a reversible 15,000° rotation and 590 revolutions per minute. A hydrostatic actuation mechanism, as seen in muscular hydrostats in nature, explains the simultaneous occurrence of lengthwise contraction and torsional rotation during the yarn volume increase caused by electrochemical double-layer charge injection. The use of a torsional yarn muscle as a mixer for a fluidic chip is demonstrated. 相似文献
80.
Yoo SH Lee KH Lee JS Cha J Park CS Lee HG 《Journal of agricultural and food chemistry》2005,53(16):6235-6239
Physicochemical characteristics and biological activities of Sphingomonas gellan (S-gellan) were investigated. The S-gellan weight fractions of Glc and GlcUA were 0.45 and 0.25, respectively, and the molar ratio of Glc:Rha:GlcUA was approximately 4:2:3. The S-gellan was chemically derivatized with diethylaminoethyl chloride-HCl (DEAE-HCl), and the resulting modified S-gellan contained both positive and negative charges. The elemental and IR analyses were conducted to confirm the successful incorporation of DEAE groups into S-gellan. A large increase in nitrogen fraction was observed from the derivatized S-gellan by elemental analysis. The IR absorption bands induced by C-H, C-N, and C-O-C stretching were noticeable at 2950, 1310-1380, and 1000-1150 cm(-1), respectively, resulting from the DEAE substitution. The characteristic CH3 and CH2 peaks originated from the DEAE group were detected in the 1H NMR spectrum of the derivatized S-gellan as well. The solubility of native S-gellan was improved almost twice from 40% to 75% after DEAE derivatization, while water holding capacity (WHC) drastically decreased from 10026% to 245%. Oil binding capacity (OBC) of S-gellan also significantly dropped from 1528% to 331% after the derivatization. The bile acid binding capacity of S-gellan was indirectly determined by measuring the holding capability of cholic acid inside the dialysis membrane (MWCO 12,000-14,000 Da). Once S-gellan was DEAE derivatized, there was substantial increase in the cholic acid retardation index (CRI). Up to 9 h of dialysis, the derivatized S-gellan released 29.3% less of cholic acid compared to the control group that did not contain S-gellan. From these results of the improved water solubility and stronger bile acid binding capacity, it would be suggested that the DEAE-derivatized S-gellan has more advantages than gellan itself for functional food applications. 相似文献