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81.
Marine derived cyclo(Gly-l-Ser-l-Pro-l-Glu) was selected as a lead to evaluate antitumor-antibiotic activity. Histidine was chosen to replace the serine residue to form cyclo(Gly-l-His-l-Pro-l-Glu). Cyclic tetrapeptides (CtetPs) were then synthesized using a solution phase method, and subjected to antitumor and antibiotic assays. The benzyl group protected CtetPs derivatives, showed better activity against antibiotic-resistant Staphylococcus aureus in the range of 60–120 μM. Benzyl group protected CtetPs 3 and 4, exhibited antitumor activity against several cell lines at a concentration of 80–108 μM. However, shortening the size of the ring to the cyclic tripeptide (CtriP) scaffold, cyclo(Gly-l-Ser-l-Pro), cyclo(Ser-l-Pro-l-Glu) and their analogues showed no antibiotic or antitumor activity. This phenomenon can be explained from their backbone structures. 相似文献
82.
M. Khudhair P. Melloy D. J. Lorenz F. Obanor E. Aitken S. Datta J. Luck G. Fitzgerald S. Chakraborty 《Plant pathology》2014,63(5):1033-1043
This study examines the CO2‐mediated influence of plant resistance on crown rot dynamics under continuous cropping of partially resistant wheat line 249 and the susceptible cultivar Tamaroi. Disease incidence, severity, deoxynivalenol and Fusarium biomass were assessed after each cycle in microcosms established at ambient and 700 mg kg?1 CO2 using soil and stubble of these wheat lines from a field experiment with free to air CO2 enrichment. Monoconidial isolates from wheat stubble were collected initially, and after five cropping cycles, to compare the frequency and aggressiveness of Fusarium species in the two populations. Aggressiveness was measured using a high‐throughput seedling bioassay. At elevated CO2, the higher initial incidence in Tamaroi increased with cropping cycles, but incidence in 249 remained unchanged. Incidence at ambient CO2 did not change for either line. Elevated CO2 induced partial resistance in Tamaroi, but not in 249. Increased Fusarium biomass in wheat tissue at elevated CO2 matched raised deoxynivalenol of the stem base in both lines. After five cycles of continuous wheat cropping, aggressiveness increased in pathogenic F. culmorum and F. pseudograminearum by 110%, but decreased in weakly pathogenic F. equiseti and F. oxysporum by 50%. CO2 and host resistance interactively influenced species frequency, and the highly aggressive F. pseudograminearum became dominant on Tamaroi irrespective of CO2 concentration, while its frequency declined on 249. This study shows that induced resistance at elevated CO2 will not reduce crown rot severity, or impede the selection and enrichment of Fusarium populations with increased aggressiveness. 相似文献
83.
84.
Rahman H Chakraborty A Deka PJ Narayan G Prager R 《Tropical animal health and production》2001,33(2):95-102
An outbreak of salmonellosis was recorded in captive pygmy hogs (Sus salvanius), a critically endangered species of mammal. Of 42 captive animals maintained for conservation breeding by the Pygmy Hog Conservation Programme, Guwahati, Assam, India, 7 (16.67%) died within 3 days. The organism associated with this outbreak was identified as Salmonella enteritidis. The organisms were highly susceptible to chloramphenicol, gentamicin, norfloxacin and cefotaxim but were resistant to ampicillin, oxytetracycline, mezlocillin and sulfamerazin. The strain belonged to phage type 13a/7 and harboured two plasmids (38 and 44 megadaltons). The organisms were enterotoxigenic in CHO cell assay and were found to carry stn, sef and pef genes. 相似文献
85.
Tapan K. Mandal Anjan Bhattacharya Animesh K. Chakraborty Dipak K. Basak 《Pest management science》1992,35(3):201-207
Disposition kinetics in goats of fenvalerate [(RS)-α-cyano-3-phenoxybenzyl (RS)-2-(4-chlorophenyl)-3-methylbutyrate] were studied after oral administration at 5 mg kg?1. The insecticide persisted in blood for up to 48 h. The Vd(area), t1/2(β), and t1/2(Ka), of fenvalerate were 12.14 (±0.39) litre kg?1, 12.25 (±0.25) h and 0.63 (±0.11)h, while the AUC and ClB values were respectively 7.35 (±0.39) μg h ml?1 and 0.68 (±0.04) litre kg?1 h?1. The residues in tissues reached a peak four days after insecticide administration and then started to decline. Maximum residue was found in the adrenal gland, followed by liver, kidney and intestine. Both GOT and GPT activities in kidney tissue, but only GPT activities in liver tissue had decreased significantly 4, 8 and 22 days post-administration. The fenvalerate did not produce any significant effects on serum acetylcholinesterase, cholesterol or protein levels in goats. Histopathological examination showed fatty changes in the periphery of lobule, congestion in sinusoid, haemolysis in central vein, necrosis and periportal fibrosis around the central vein of liver, and necrosis in kidney of fenvalerate-treated goats. 相似文献
86.
ABSTRACT Pathogenic variation in Colletotrichum gloeosporioides infecting species of the tropical pasture legume Stylosanthes at its center of diversity was determined from 296 isolates collected from wild host population and selected germ plasm of S. capitata, S. guianensis, S. scabra, and S. macrocephala in Brazil. A putative host differential set comprising 11 accessions was selected from a bioassay of 18 isolates on 19 host accessions using principal component analysis. A similar analysis of anthracnose severity data for a subset of 195 isolates on the 11 differentials indicated that an adequate summary of pathogenic variation could be obtained using only five of these differentials. Of the five differentials, S. seabrana 'Primar' was resistant and S. scabra 'Fitzroy' was susceptible to most isolates. A cluster analysis was used to determine eight natural race clusters using the 195 isolates. Linear discriminant functions were developed for eight race clusters using the 195 isolates as the training data set, and these were applied to classify a test data set of the remaining 101 isolates. All except 11 isolates of the test data set were classified into one of the eight race clusters. Over 10% of the 296 isolates were weakly pathogenic to all five differentials and another 40% were virulent on just one differential. The unclassified isolates represent six new races with unique virulence combinations, of which one isolate is virulent on all five differentials. The majority of isolates came from six field sites, and Shannon's index of diversity indicated considerable variation between sites. Pathogenic diversity was extensive at three sites where selected germ plasm were under evaluation, and complex race clusters and unclassified isolates representing new races were more prevalent at these sites compared with sites containing wild Stylosanthes populations. 相似文献
87.
Kajal Chakraborty Anusree Maneesh Fasina Makkar 《Journal Of Aquatic Food Product Technology》2017,26(4):406-419
Antioxidant potential of three brown seaweeds, Anthophycus longifolius, Sargassum plagiophyllum, and Sargassum myriocystum, obtained from the Gulf of Mannar region of India were evaluated utilizing different in vitro systems. Ethyl acetate (EtOAc) fraction of Anthophycus longifolius registered significantly greater hydroxyl radical scavenging ability (IC50 0.19 mg/mL) and was effective in stabilizing the 2,2′-azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (IC50 1.23 mg/mL) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radicals (IC50 0.48 mg/mL) (p < 0.05). No significant differences in hydrogen peroxide (H2O2) scavenging and ferrous ion chelating properties of the EtOAc extracts of the seaweeds were apparent. The utilities of the reverse-phase high-performance liquid chromatography (RP-HPLC) method hyphenated to diode-array detection for analyzing the fingerprints of phenolic constituents in the solvent extracts and fractions of the seaweeds were denoted. High-performance liquid chromatographic analysis indicated the presence of phenolic acids in the solvent extracts of seaweeds. This study demonstrated the potential use of A. longifolius as candidate species to be used as a nutritional food supplement/functional foods to increase the shelf life of food items for human consumption. 相似文献
88.
Unniyampurath Unnikrishnan Kajal Chakraborty Rajaian Paulraj 《Aquaculture Research》2010,41(10):1498-1513
Efficacy of sunflower oil (diet SF) and soybean oil (diet SB) alone and in combination with cod liver oil (diets M1‐2.80:1.40:1.40, M2‐2.80:2.24:0.56 and M3‐2.80:0.56:2.24; cod liver oil:sunflower oil:soybean oil) as lipid supplements (5.6%) in formulated diets (crude fat ~9.79%) for juvenile Scylla serrata (weight=0.28±0.07 g, carapace width=9.7±0.1 mm) were compared with diet CL, containing cod liver oil alone as the lipid supplement (6 diets × 24 crabs stocked individually, randomized block design). Growth performance, nutrient (protein and lipid) intake and gain of crabs fed M1, M2 and M3 were higher (P≤0.05) than the crabs fed SF and SB, but were not significantly different (P≥0.05) from crabs fed CL. Dietary fatty acids (FAs) are found to influence the FA profile of test crabs. Higher tissue levels of 16:1n‐7, 18:1n‐9 and 18:1n‐7 reflected the essential FA deficiency in crabs fed diets supplemented only with vegetable oils. Results confirmed that S. serrata could utilize vegetable oil supplements in the formulated diets as a partial replacement (50%) of cod liver oil without compromising growth and survival. Partial substitution of marine fish oil with suitable vegetable oils can reduce the feed cost considerably, in the context of rising fish oil prices. 相似文献
89.
Swapan Chakrabarty Minghui Jin Chao Wu Panchali Chakraborty Yutao Xiao 《Pest management science》2020,76(5):1612-1617
Vip3A proteins are widely used for controlling pest Lepidoptera. Different binding sites with different receptors in the insect midgut membrane and lack of cross‐resistance with crystal (Cry) proteins enhance their applicability, as both single proteins and proteins pyramided with Cry proteins in transgenic Bt crops. Vip3A proteins are effective but there is relatively little information about their structure, function, activation, specificity, and mode of action. In addition, the mechanism of insect resistance to these proteins is unknown. Phylogenetic analysis and multiple sequence alignment showed that Vip3A proteins are genetically distant from Cry proteins. The mode of action and insecticidal activity of Vip3A proteins are discussed in this review. This review also provides detailed information about the Vip3A protein family that may aid in the design of more efficient pest management strategies in response to insect resistance to insecticidal proteins. © 2020 Society of Chemical Industry 相似文献
90.
P. L. Lalrinsanga S. K. Chakraborty Alkesh Dwivedi Nimai Barman Niraj Kumar 《Journal Of Applied Aquaculture》2013,25(4):250-262
The effects of cryoprotectants and their concentrations, prefreezing temperature, equilibration time, thawing temperature, and the regenerative efficacy of cryopreserved vegetative thalli (apical tips) of Gracilaria corticata J. Agardh., Ulva lobata Duetzing, and Hypnea musiformis (Wulfar) Lamouroux were evaluated. The apical tips were suspended in various cryoprotective solutions and slowly cooled to ?40?C over a period of 4 h. After the slow cooling step, the suspensions were immediately immersed in liquid nitrogen (LN), which were thawed after 2 days. Both U. lobata and H. musiformis survived maximally in 10% DMSO, whereas 10% glycerol was found most suitable for G. corticata. A slow cooling temperature of ?40?C and thawing temperature of 40?C were found most suitable irrespective of the species. The equilibration time of 60 min was found most suitable for G. corticata, while 45 min was sufficient for both H. musiformi and U. lobata. Cryopreserved vegetative thalli were then successfully regenerated, though the survival was considerably reduced up to a period of 28 days after which it was stabilized. 相似文献