Expression patterns of natriuretic peptides in pre-hibernating and hibernating Anatolian ground squirrel (Spermophilus xanthoprymnus) kidney

Veterinary Research Communications - Hibernation is characterized by marked suppression of renal function. Natriuretic peptides (NPs) are involved in the regulation of renal function. However, the...     

In vitro proliferation and minimum growth storage of fraser photinia: Influences of different medium,sugar combinations and culture vessels

Protocols for the in vitro proliferation and storage of fraser photinia were developed by comparing 6-benzyladenine (BA) concentrations (0.5–4 mg/L) together with different media formulations [Murashige and Skoog (MS) media and Quoirin and Lepoivre (QL) media], sugar combinations (sucrose and mannitol), culture vessels (baby food jars and vitrovents) and methods (synthetic seed technology and slow growth storage). The best responses in terms of both proliferation percentage and multiple shoot formation were obtained in QL medium containing 1 mg/L BA. Synthetic seed production was optimized by encapsulating shoot apices in 3% sodium alginate. Encapsulated shoot apices could be maintained up to 6 months at 4 °C in dark with 91.6% sprouting in MS medium. Microshoots were stored at 4 °C up to 15 months on sucrose and mannitol containing QL medium in both baby food jars and vitrovents without subculture. The stored material could be recovered and multiplied normally in 1 mg/L BA supplemented QL medium. Both in vitro propagated and conserved microshoots were rooted (∼75%) on QL medium with 1 mg/L indole butyric acid (IBA). Optimized synthetic seed and slow growth storage system can be used for short and medium-term storage of fraser photinia germplasm.     

Nutritional quality of amino acid in farmed,farm‐aggregated and wild Axillary seabream (Pagellus acarne) with implications to Human Health

This study evaluated the nutritional quality of farmed and wild axillary seabream (Pagellus acarne R., 1827) focusing on amino acid profiles, with regards to possible interactions with wild fish aggregating around the cage facility. Total amino acids (∑AA), essential amino acids (∑EAA), non‐essential amino acids (∑NEAA) and neutral amino acids (∑NAA) in farmed fish were lower than those in the wild individuals (p > .05). Amino acid pattern in cage‐aggregated fish showed a slight decline from the wild populations, but still higher than the farmed fish. Based on the amino acid scores (AAS), lysine and leucine could be underlined as the ‘first limiting amino acids’, but all other AASs were over ‘1’, in accordance with reference amino acid contents of FAO/WHO (>1.00), showing that farmed axillary seabream provides high nutritional quality and can be considered as a favourable protein source. The ratios of ∑EAA/∑AA (44%–46%) and ∑EAA/∑NEAA (79%–86%) exceeded the minimum recommendation of 40% and >60% by FAO/WHO for all three groups. It can be concluded that axillary seabream either farmed, farm‐aggregated or distant wild fish presented high‐quality protein generating a healthy source for human food.     

Effect of seasonal water stress imposed on drip irrigated second crop watermelon grown in semi-arid climatic conditions

A study was conducted to determine the water stress effect on yield and some physiological parameters including crop water stress index for drip irrigated second crop watermelon. Irrigations were scheduled based on replenishment of 100, 75, 50, 25, and 0% soil water depletion from 90 cm soil depth with 3-day irrigation interval. Seasonal crop evapotranspiration (ET) for I₁₀₀, I₇₅, I₅₀, I₂₅, and I₀ were 660, 525, 396, 210, and 70 mm in 2003 and 677, 529, 405, 221, and 75 mm in 2004. Fruit yield was significantly lowered by irrigation water stress. Average water-yield response factor for both of the years was 1.14. The highest yield was obtained from full irrigated treatment as 34.5 and 38.2 t ha⁻¹ in 2003 and 2004, respectively. Lower ET rates and irrigation amounts in water stress treatments resulted in reductions in all measured parameters, except water-soluble dry matter concentrations (SDM). Canopy dry weights, leaf relative water content, and total leaf chlorophyll content were significantly lowered by water stress. Yield and seasonal ET were linearly correlated with mean CWSI values. An average threshold CWSI value of 0.17 before irrigation produced the maximum yield and it could be used to initiate the irrigation for watermelon.     

Using Artificial Neural Network and Multiple Linear Regression for Predicting the Chlorophyll Concentration Index of Saint John’s Wort Leaves

This research investigates and compares artificial neural network and multiple linear regression for predicting the chlorophyll concentration index of Saint John’s wort leaves (Hypericum perforatum L.). Plants were fertilized with 0, 30, 60, 90, and 120 kg ha^?1 nitrogen [34% nitrogen ammonium nitrate (NH₄NO₃)]. Chlorophyll concentration index of each leaf was measured using SPAD meter. Afterwards, rgb (red, green, and blue color) values of all leaf images were determined by image processing. Values obtained were modeled using both multiple regression analysis and artificial neural networks. Using multiple regression analysis R² values were between 0.61 and 0.97. Coefficient of determination values (R²) using artificial neutral network values were found to be 0.99. Artificial neutral network modeling successfully described the relationship between actual chlorophyll concentration index values and predicted chlorophyll concentration index values.     

Meat quality characteristics of Turkish indigenous Hair goat kids reared under traditional extensive production system: effects of slaughter age and gender

Meat quality characteristics of Turkish indigenous Hair goat kids reared under an extensive production system were investigated in this study. A total of 60 Hair goat kids (30 females and 30 males) were slaughtered at 3, 6, 9, and 12 months of ages. Slaughter age significantly affected meat quality characteristics. Cooking loss (P?<?0.01) and shear force value (P?<?0.001) increased with age. Meat color became darker red with increasing slaughter age (P?<?0.001). The percentage of fat of musculus (M.) semimembranosus increased with age (P?<?0.01). The percentage of saturated fatty acids was significantly higher (P?<?0.01), whereas the percentage of unsaturated fatty acids was lower (P?<?0.001) in the M. longissimus dorsi of the kids slaughtered at 3 months of age than those in the other age groups. The females had higher intramuscular fat than that in males (P?<?0.001) and meat from male kids contained a higher percentage of polyunsaturated fatty acid than that from females (P?<?0.01). In conclusion, meat from Hair kids slaughtered between 3 and 9 months of ages had better quality than those slaughtered at the other ages and also meat from male kids had better quality than those female kids slaughtered at the same age under extensive production system.     

Evaluation of dietary protein and lipid requirements of two-banded seabream (Diplodus vulgaris) cultured in a recirculating aquaculture system

The objective of this study is to investigate the effects of dietary protein and lipid levels on the growth performance and bioeconomic benefits of two-banded seabream (Diplodus vulgaris) juveniles, a candidate species for aquaculture sector. Eight experimental diets were formulated with four protein (50, 45, 40 and 35 %) levels for each of the two lipid levels (15 and 10 %). Triplicate groups of juvenile fish with an average initial body weight of ~3.64 g were reared in a recirculating aquaculture system and hand fed twice a day until satiation for a period of 60 days. In the experiment, no difference in survival rate was found between the different groups. Relative growth rate (RGR), specific growth rate (SGR), feed conversion ratio (FCR) and daily feed intake were not significantly affected by increasing protein and/or lipid treatments in this present study. However, the RGR, SGR and FCR values showed slightly better efficiency in the experimental group (35/15) fed with lower protein content (35 %) and higher lipid level (15 %) compared with those fed other diets. According to bioeconomic analyses results, the diet with the 35 % protein and 15 % lipid generated the best profit. The results suggest that two-banded seabream can be accepted as a promising alternative species for the aquaculture industry and optimum growth of two-banded seabream fingerlings can be obtained when they are fed a diet containing 35 % crude protein and 15 % crude lipid.