Chemical composition,in vitro gas production,methane production and fatty acid profile of canola silage (Brassica napus) with four levels of molasses

The objective of this study was to investigate the effect of four levels of molasses on chemical composition, in vitro digestibility, methane production and fatty acid profile of canola silages. A canola (Brassica napus var. Monty) crop was established in a small-scale agricultural farm and harvested 148 days after sowing. Four levels of molasses were tested with respect to the fresh weight (1.5 kg); these were 1% (CS-1), 2% (CS-2), 3% (CS-3) and 4% (CS-4) molasses, and 0% molasses (CS-0) was included as a control. A total of 45 microsilages were prepared using PVC pipes (4 in. of diameter × 20 cm of length), and the forage was compressed using a manual press. The effects of control and treatments were tested using the general linear model Y?=?μ?+?T_i?+?E_ij. The linolenic acid (C18:3n3), palmitic acid (C16:0) and linoleic acid methyl ester (C18:2n6c) accounted for 30%, 21% and 10.5% of total fatty acids, respectively; the fermentation parameters and in vitro methane production were not affected (P?>?0.05) by treatments; in vitro digestibility decreased significantly (P?<?0.05) as the level of molasses increased. It was concluded that CS-4 improved the DM content by 9% and showed high content of linolenic acid methyl ester. The gross energy of canola silages could favour the oleic acid methyl ester.

     

Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements

Background: Increasing understanding on the functions of amino acids(AA) has led to new commercial applications and expansion of the worldwide markets. However, the current technologies rely heavily on non-food grade microorganism and chemical synthesis for the production of AA. Several studies reported that lactic acid bacteria(LAB) have the capability of producing AA owing to their well-established proteolytic system and amino acid biosynthesis genes. Hence, the objectives of this study were to explore the extracellular proteolytic activity of LAB isolated from various Malaysian fermented foods and their potential to produce AA extracellularly as feed supplements.Results: Al the studied LAB isolates were versatile extracel ular protease producers, whereby extracel ular protease activities were detected from acidic to alkaline pH(pH 5, pH 6.5, pH 8) using qualitative and quantitative proteolytic assays.The highest proteolytic activity at pH 5(15.76 U/mg) and pH 8(19.42 U/mg) was achieved by Lactobacil us plantarum RG14, while Lactobacil us plantarum RS5 exhibited the highest proteolytic activity of 17.22 U/mg at pH 6.5. As for the results of AA production conducted in de Man, Rogosa and Sharpe medium and analysed by high pressure liquid chromatography system, al LAB isolates were capable of producing an array of AA. General y, Pediococcus sp. showed greater ability for AA production as compared to Lactobacil us sp. Moreover, the studied LAB were able to produce a few major feed supplement AA such as methionine, lysine, threonine and tryptophan. P. pentosaceus TL-3 recorded the highest methionine and threonine productivity of 3.72 mg/L/h and 5.58 mg/L/h respectively. However, L. plantarum I-UL4 demonstrated a lysine productivity of 1.24 mg/L/h, while P. acidilactici TP-6 achieved up to 1.73 mg/L/h of tryptophan productivity.Conclusion: Al the 17 studied LAB isolates possessed versatile extracel ular proteolytic system and have vast capability of producing various amino acids including a few major feed supplement AA such as methionine, lysine, threonine and tryptophan. Despite AA production was strain dependent, the studied LAB isolates possessed vast potential and can be exploited further as a bio-agent or an alternative amino acids and bioactive peptide producers.     

IMAGING DIAGNOSIS—NUCLEAR SCINTIGRAPHIC,RADIOLOGICAL, AND PATHOLOGIC CHARACTERISTICS OF METASTATIC PILOMATRICOMA IN A DOG

A 6‐year‐old castrated Goldendoodle dog was presented for left‐sided lameness of 3 weeks’ duration. Focal, moderate to marked increased ^99mTc‐methylene diphosphonate (^99mTc‐MDP) uptake was detected in the right caudal lung lobe, caudal angle of the left scapula, and the distal aspect of the left femur with whole body bone phase scintigraphy. Radiographs identified a well‐circumscribed, oval‐shaped soft tissue opaque mass in the right caudal lung lobe; a suspect oval‐shaped osteolytic lesion in the proximal third of the left scapula; and an osteolytic lesion in the distal aspect of the left femur. Metastatic pilomatricoma was confirmed histologically at all three sites.     

In situ and in vitro antioxidant activity of sweetpotato anthocyanins

Anthocyanins from a variety of fruits and vegetables have been shown to possess potent antioxidant activity in vitro, but scavenging of free radicals by anthocyanins has only been demonstrated in situ in the leaves of certain plants. We report on a new sweetpotato that exhibits mottled purple flesh attributable to high concentrations of anthocyanins. By perfusing transverse sweetpotato sections with the reactive oxygen species H(2)O(2), followed by the H(2)O(2) sensitive fluorochrome scopletin, we show that anthocyanins act as antioxidants in situ within the sweetpotato storage roots. We also demonstrate in vitro antioxidant activity by sweetpotato anthocyanins, where an additive effect with hydroxycinnamic acids is observed. Anthocyanic foods have been shown to offer protection against a variety of degenerative disease processes. Given that sweetpotato can be eaten several hundred grams at a time and as a staple, these data are consistent with the possibility of superior health protection by anthocyanic varieties of sweetpotato in comparison to most common fruits and vegetables.     

Use of refined potato (Solanum tuberosum L. cv. Gogu valley) protein as an alternative to antibiotics in weanling pigs

This study investigates the use of refined potato protein (RPP) obtained from the potato (Solanum tuberosum) tubers of “Gogu valley” for their antimicrobial properties and its effects on growth performance, apparent nutrient digestibility and microbial populations in feces and large intestine of weanling pigs. Pigs (n = 280; Landrace × Yorkshire × Duroc; average initial body weight of 5.96 ± 0.66 kg; 23 ± 3 d of age) were allotted on the basis of their body weight and sex to five dietary treatments (each treatment comprised of 4 replicate pens with 14 pigs in each) in a randomized complete block design. The dietary treatments were: PC (positive control; basal diet + 150 ppm apramycin sulfate and 10 ppm colistin sulfate), and RPP (basal diet added with 0, 200, 400 and 600 ppm RPP). The RPP showed in vitro antimicrobial activity and at the concentration of 150 ppm inhibited the growth of tested microbes (Staphylococcus aureus, Salmonella choleraesuis, Salmonella gallinarum and Escherichia coli). Pigs fed with antibiotic diet showed better growth performance and had lower populations of total bacteria, coliforms and Staphylococcus spp. in the feces and large intestine when compared with pigs fed RPP diets. Increasing the levels of RPP in diets linearly improved performance and reduced the populations of total bacteria, coliforms and Staphylococcus spp. in feces and contents of colon and rectum. The apparent fecal digestibility of DM, CP and apparent ileal digestibility of amino acids did not differ among pigs fed antibiotic and RPP diets. These results suggest that antibiotics were more effective in improving the performance of pigs, while feeding of RPP also improved the performance and reduced the harmful microbes being more effective at higher levels. Thus at higher levels RPP obtained from the potato tubers of Gogu valley can be a potential replacement of antibiotics in the feed of weanling pigs.     

Seasonal changes in durian leaf and soil mineral nutrient element content

The 3–4 year monitoring study showed that fluctuations in durian (Durio zibethintts Murray) leaf and soil nutrient element levels were closely related to seasonal changes in the crop phenology. The leaf sampling method was standardized taking the 5th and 6th mature green leaf from the shoot tip. The best sampling time was determined to be in November where the coefficient of variability for most nutrient elements were the lowest at or lower than 20%. Tentative standards for durian leaf nutrient elements were set up taking the 95% confidence interval of the means at this sampling stage. All the leaf macro elements, nitrogen (N), phosphorus (P), potassium (K), sulfur (S), and the micro elements, zinc (Zn), manganese (Mn), copper (Cu), and iron (Fe) declined or were lower during fruit set and development from September to end of fruit harvest in January. Leaf N was also the lowest in July and leaf P at the tail end of harvest. Soil N, P, and the bases K, Ca, and Mg also exhibited similar trends, lower during fruit development especially in October‐November and were lower during active leaf flushing from March to May.