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901.
Recruitment and movement of the hard clam Meretrix lusoria in a tidal river of northern Kyushu,Japan
Yasuhisa Henmi Satoshi Kobayashi Junpei Yamaguchi Masahiro Hashiguchi 《Fisheries Science》2014,80(4):705-714
Size-frequency distributions of Meretrix lusoria, a commercially important bivalve in Japan, were compared among 6 sites in tidal flats of a small river in Kafuri Bay, northern Kyushu, Japan during 2006–2008. In Kafuri Bay, the hard clam resource is strictly managed and mean biomass of this species on the fishing ground (about 1.5 km2) exceeded 1.5 kg m?2 in 2008. Small clams (<5 mm in shell length, SL) were most abundant in the border area between the river and the sea, while large clams (>40 mm SL) were rare there. In contrast, in the lower site of the marine area (site L), most clams were >30 mm SL. These results indicate that small clams move from riverine to marine areas with growth (post-settlement movement). Year-round, very small clams (<1 mm SL) were present and clams that were 2–5 mm SL were very rare, although the spawning season is thought to occur in summer. Probably, the early growth of many new recruits is very slow and most may even be <1 mm SL in the next spring, although some clams grow to >5 mm SL in a short period of time. 相似文献
902.
Haruna Amano Makiko Kitamura Toshiaki Fujita Naoshi Hiramatsu Takashi Todo Satoshi Suyama Akihiko Hara 《Fisheries Science》2008,74(4):830-836
ABSTRACT: Lipovitellin (Lv), the major yolk protein derived from vitellogenin (Vg), was purified from vitellogenic ovaries of Pacific saury Cololabis saira using hydroxylapatite column chromatography followed by gel filtration. The apparent native mass of purified Lv was approximately 420 kDa, while the tertiary structure of Lv revealed by sodium dodecylsulfate–polyacrylamide gel electrophoresis was typical of teleost Lvs, consisting of a heavy chain (∼99 kDa) and a light chain (∼34 kDa). Western blot analysis using rabbit antiserum raised against Pacific saury Lv revealed a specific reaction with a polypeptide (∼194 kDa) that is present in serum from female Pacific saury but not in male serum, suggesting the approximately 194-kDa polypeptide to be the Vg monomer. This study describes the first step toward the development of specific immunoassays for Pacific saury Vg, which will be an effective tool for monitoring the reproductive development of this species. 相似文献
903.
Yasuzumi?FujimoriEmail author Kenji?Chiba Tatsuki?Oshima Kazushi?Miyashita Satoshi?Honda 《Fisheries Science》2005,71(4):738-747
ABSTRACT: Variations in trawl dimension, bottom contact, and catch with differing warp lengths during walleye pollock surveys conducted by the Fisheries Research Agency were examined. The ratio of warp length to fishing depth (scope ratio) was set at 2.5, 3.0 and 3.5 at depths of 110 m. At a 2.5 scope ratio, the net mouth shape and footgear contact fluctuated, as the trawl door did not contact the bottom. Footgear contact was complete when the scope ratio was 3.0 or more. Walleye pollock, sculpins and flatfishes were the main catch in all tows, and the catch increased with scope ratio. There was no difference in the length frequency of walleye pollock ( n = 300) captured at 2.5 and 3.0 scope ratio. However, the length frequency at 3.5 was significantly different from that of other scope ratios. These results suggest that at a scope ratio of 3.0 or more, the trawl door will not leave the bottom at any depths. To compliment echo surveys for walleye pollock, a 3.0 scope ratio would be optimal, since the catch data for a 3.5 scope ratio was dissimilar from that of scope ratios. 相似文献
904.
Nam -Il Won Tomohiko Kawamura Toshihiro Onitsuka Jun Hayakawa Satoshi Watanabe Toyomitsu Horii Hideki Takami Yoshiro Watanabe 《Fisheries Science》2007,73(5):1123-1136
Abstract: The community and trophic structures of the subtidal habitat of the abalone Haliotis diversicolor were examined in Nagai, Sagami Bay, Japan. Conventional community indices showed no significant differences among three sampling stations. The overall continuum of stable isotope ratios was structured into three different trophic linkages: (i) brown algae-dependent benthic food chain; (ii) red algae-dependent benthic food chain; and (iii) planktonic food chain. Brown algae and red algae likely play different roles with respect to carbon sources in the habitat. Conventional fractionation values indicated that the abalone H. diversicolor (δ13 C = −12.4 ± 1.0‰, δ15 N = 9.3 ± 0.5‰) feeds on the lamina of Undaria pinnatifida during juvenile and adult stages (8.0–65.6 mm shell length). Stable isotope signatures suggested that the juveniles of other abalone species as well as some amphipods and a sea cucumber Holothuria decorata are competitors of H. diversicolor , whereas some Muricidae gastropods such as Thais bronni and Ergalatax contractus are predators. The isotopic differences among macroalgal species and the subsequent transfer to consumers indicate that stable isotopic analysis is an effective means of studying food webs in an open rocky shore community with little influence from external primary production such as terrestrial vegetation. 相似文献
905.
Mina Hori Satoshi Ishikawa Ponley Heng Somony Thay Vuthy Ly Thuok Nao Hisashi Kurokura 《Fisheries Science》2006,72(4):846-854
ABSTRACT: Household surveys of small-scale fishing were conducted in Svay Ear and Srey Rangit villages in Kompong Thom Province, Cambodia. Data were collected from 104 of 183 households and 105 of 140 households in Svay Ear and Srey Rangit, respectively. In both villages, almost all households conducted rice cultivation and approximately 75% of them also conducted fishing. Three fishing groups: (i) inside lake; (ii) around lake; and (iii) around village were observed in both villages, according to their main fishing grounds. The inside lake group conduct fishing only in Tonle Sap Lake which is located 30 km away from the villages. The around lake group fish in lakes, marshes and flooded forests adjacent to Tonle Sap Lake, which are more than 5 km from the villages. The around village group collects fish from rice fields and ponds located inside the villages and also in some streams, rivers and lakes adjacent to the villages. The distance to the fishing grounds is less than 5 km from the villages. Most fishing groups were the inside and around lake groups, which occupied 76.0 and 65.7% of Svay Ear and Srey Rangit, respectively. These two groups produced 80.6 and 69.7% of the respective village annual total fish catches. These two groups sold a large portion of their fish catches. Although previous reports indicated that small-scale fishing is for subsistence, present results indicated that small-scale fishing was commercially conducted and fishing was important as an income source. 相似文献
906.
Michiru Shiga Daisuke Shiode Satoshi Hayashi Tadashi Tokai Fuxiang Hu 《Fisheries Science》2008,74(3):479-487
ABSTRACT: A midwater float is a small float attached to the mainline of pelagic longlines to standardize the hook depth. In this study theoretical equations are presented for estimating the buoyancy of the midwater float required to lift the joints of the midwater float line on the mainline to the target depth. Sea trials using full scale tuna longline gear with midwater floats were carried out in the Indian Ocean in December 2004 and 2005, in order to examine the validity of the theoretical equations. In the sea trials, two types of midwater float settings, single midwater float setting and double midwater float setting, were tested and compared with the conventional setting. As a result, the joints of the midwater float line on the mainline were successfully lifted to the target depth as expected, demonstrating the validity of the theoretical equation. The range of hook depths in the midwater float setting was less spread over depths than in the conventional setting, and therefore, use of long float lines (100 m) with the midwater float setting allows all hooks to avoid entering the sea turtle habitat of shallower than 100 m depth. Factors affecting shoaling of the longline with the midwater float are also discussed. 相似文献
907.
Genomic evaluation using SNP‐ and haplotype‐based genomic relationship matrices in a closed line of Duroc pigs
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Yoshinobu Uemoto Shuji Sato Takashi Kikuchi Sachiko Egawa Kimiko Kohira Hironori Sakuma Satoshi Miyashita Shinji Arata Takatoshi Kojima Keiichi Suzuki 《Animal Science Journal》2017,88(10):1465-1474
A simulation analysis and real phenotype analysis were performed to evaluate the impact of three different relationship matrices on heritability estimation and prediction accuracy in a closed‐line breeding of Duroc pigs. The numerator relationship matrix (NRM), single nucleotide polymorphism (SNP)‐based genomic relationship matrix (GRM) (GS), and haplotype‐based GRM (GH) were applied in this study. We used PorcineSNP60 genotype array data (38 114 SNPs) of 831 Duroc pigs with four selection traits. In both heritability estimation and prediction accuracy, the accuracy depended on the number of animals with records. For heritability estimation, a large difference in the results among three relationship matrices was not shown, but the trend of the estimated heritabilities between GRMs, that is GS < GH, was shown in this population. For the accuracy of prediction values in test animals, the accuracies of prediction values obtained by two GRMs were higher than that by the NRM in this population. The accuracies obtained by GRMs using animals with no records were lower than that by the NRM using animals with their performance records, but were close to that by the NRM using animals with full‐sib testing records. 相似文献
908.
Yokohira M Hashimoto N Yamakawa K Suzuki S Saoo K Kuno T Imaida K 《Journal of toxicologic pathology》2009,22(3):179-185
The purpose of the present study was to investigate the effects of inflammation, induced by intratracheal instillation (i.t.) of quartz as an environmental factor in the lung or drinking of dextran sulfate sodium (DSS) as an environmental factor in the colon on lung tumors in female A/J mice initiated with NNK. For comparison, colonic preneoplastic lesions, aberrant crypt foci (ACF), were also assessed. A/J mice at 6 weeks of age were divided into 5 groups, and Groups 1, 2 and 3 were pretreated with NNK (2 mg / 0.1 ml saline / mouse, intraperitoneal injection) at week 0. For a week, 2% DSS in drinking water was administered to the mice in Groups 2 and 4 beginning in week 1. In week 2, the mice of Groups 3 and 5 were exposed to intratracheal instillation of quartz (0.1 mg/rat) suspended in 25 μl saline. The experiment was terminated after 16 weeks. The results for the lung tumors and colonic ACFs showed a lack of modifying effects of the inflammation in either site. Hematologically and histopathologically, the inflammation induced by 0.1 mg quartz in the lung and 2% DSS in the colon was lacking or only mild at the end of 16 weeks. These results suggest that there may be differences in sensitivity to inflammation that determine tumor promoting potential. 相似文献
909.
Satoshi Furukawa Yuichiro Machida Kazuya Takeuchi Yumiko Hoshikawa Kota Irie 《Journal of toxicologic pathology》2022,35(3):237
In order to elucidate the effects of swim bladder inflation failure on swim bladder carcinogenesis, we investigated the sequential histopathological changes of swim bladders at 13, 24, 35, and 53 days post-hatch (dph) in medakas with an uninflated swim bladder, which was experimentally induced by denying access to the air–water interface between 0 and 6 dph. The reactive oxygen species (ROS) levels were measured at 24 dph. An uninflated swim bladder was induced in 47.3% of the fish denied access to the air–water interface (the denied group). The total incidence of swim bladder adenoma was 54.1% in the denied group; however, these tumors were observed in all fish with an uninflated swim bladder. In fact, these tumors were observed from 13 dph and onwards. The TBARS levels of the juveniles showed a 2.6-fold increase in fish with an uninflated swim bladder in the denied group compared to that in the control group. It is speculated that swim bladder inflation failure has some effects on the gas gland to produce ROS, leading to DNA damage in the gas glandular epithelium, which develops into swim bladder adenomas. Consequently, it is concluded that denying access to the air-water interface between 0 and 6 dph in medaka is an easy method of inducing swim bladder tumors in a short-term period, and is a useful method for producing tumor-bearing fish. 相似文献
910.
Hieu Duc DUONG Yuji TANIGUCHI Yasuhiro TAKASHIMA Satoshi SEKIGUCHI Khin Myo AYE Parnian AHMADI Linh Khanh BUI Takao IRIE Eiji NAGAYASU Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):905
Toxoplasmosis is a widespread protozoan zoonosis. Since ingesting undercooked meat harboring Toxoplasma gondii cyst is considered one of the major transmission routes to humans, the screening of T. gondii in meat-producing animals can reduce the risk of food-borne toxoplasmosis in humans. Among serological diagnostic methods, Luciferase-linked Antibody Capture Assay (LACA) has been found to be a promising platform with high sensitivity and specificity. In this study, we aimed to evaluate recombinant nanoluciferase fused-T. gondii antigens (rNluc-GRA6, rNluc-GRA7, rNluc-GRA8 and rNluc-BAG1) for their potential use in LACA for pigs. As a result, the sensitivity of GRA6-, GRA7-, GRA8- and BAG1-LACA were 70.0%, 80.0%, 80.0% and 30.0% with specificity 87.0%, 81.5%, 74.1% and 50.0%, respectively. The cocktail LACA using a mixture of rNluc-GRA6, rNluc-GRA7 and rNluc-GRA8 indicated higher sensitivity (90.0%) and a similar specificity (96.3%) in comparison with the commercial ELISA kit. Compared to the Dye-Test as a reference test, cocktail LACA showed strong agreement (kappa value=0.811) when we assessed pig sera collected at the slaughterhouse. In addition, we also successfully established the rapid LACA format for the detection of Toxoplasma infection in pigs (called Rapid-LACA) in which the test could be performed within 30 min. In Rapid-LACA, the protein A pre-coated/blocked plates could be preserved at −30°C, 4°C or room temperature conditions for at least two months without compromising on the quality of assay. 相似文献