Assessing the role of landscape connectivity in recent woodpecker range expansion in Mediterranean Europe: forest management implications

Woodpecker species have significantly expanded their ranges in the last decades of the twentieth century in Mediterranean Europe, which seems to be closely related to forest maturation following large-scale decline in traditional uses. Here we assess the explicit role of forest landscape connectivity in the colonization of the Great Spotted Woodpecker (Dendrocopos major) and the Black Woodpecker (Dryocopus martius) in Catalonia (NE Spain). For this purpose we combined data on breeding bird atlas (10 × 10 km; 1980–2000) and forest inventories (c. 1 × 1 km, 2000). Forest connectivity was measured through graph theory and habitat availability metrics (inter- and intra-patch connectivity) according to species median natal dispersal distances. The best regressions from a set of alternative models were selected based on AICc. Results showed that connectivity between areas of mature forests [diameter at breast height (dbh) ≥ 35 cm] affected Black Woodpecker colonization events. The probability of colonization of the Great Spotted Woodpecker was greater at localities near the sources of colonization in 1980 and with a high connectivity with other less developed forest patches (dbh < 35 cm). The spatial grain at which landscape connectivity was measured influenced the model performance according to the species dispersal abilities, with the species with the lower mobility (D. major) responding better to the forest connectivity patterns at finer spatial scales. Overall, it seems that both species could expand further in European Mediterranean forests in upcoming years but at slower rates if landscape connectivity according to species requirements does not continue to increase. Hence, a proactive and adaptive management should be carried out in order to preserve these species while considering the related major impacts of global change in Mediterranean Europe.     

Weed control and crop tolerance to propane flaming as influenced by the time of day

Time of day has been observed to affect flaming efficacy. The basis for the differential plant response is not well understood; however, daily variation in leaf relative water content (RWC) is thought to contribute to the response. Leaf RWC is the ratio of the amount of water in the leaf tissue compared to when fully turgid. To determine the influence of leaf RWC in plant response to propane flaming, greenhouse experiments were conducted during April and repeated in September of 2009. Two crops [4-leaf maize (Zea mays) and second trifoliate soybean (Glycine max)] and two weed species [5-leaf velvetleaf (Abutilon theophrasti) and 6-leaf green foxtail (Setaria viridis)] were flamed with four propane doses of 0, 29, 43 and 87 kg ha⁻¹ at 0, 4, 8 and 12 h after sunrise-HAS. Leaf RWC was measured before treatment application. Flaming treatment was conducted utilizing a hand flamer with one VT 2-23 C vapor phase burner positioned 20 cm above soil surface and angled horizontally at 30°. The propane pressure was 120 kPa and the application speeds were 1.6, 3.2 and 4.8 km h⁻¹. The plant responses evaluated were plant injury and fresh weight at 7 days after treatment (DAT). All plant species were more susceptible to flaming during the afternoon when they had lower leaf RWC at 8 HAS; however, the response of these plants did not differ with the plants flamed at 12 HAS. Green foxtail flamed at 87 kg ha⁻¹ at 0, 8 and 12 HAS had injury of 62, 76 and 82%, respectively. The same response was observed in velvetleaf which had 80% injury when flamed with 87 kg ha⁻¹ at 0 HAS and 93% injury when flamed at 12 HAS with the same propane dose at 7 DAT. Similar trends occurred for maize and soybean suggesting that leaf RWC could be one of the factors affecting plant response to flaming. Practical implication is that flaming operation should be conducted in the afternoon in order to improve efficacy of weed control and reduce propane consumption rate.     

Occurrence of Fusarium species in maize kernels grown in northwestern Spain

Fusarium poses food and feed safety problems because most species produce mycotoxins. To understand the epidemiology of the Fusarium disease, efforts must focus more precisely on how environmental variables affect disease presence. The objectives of the present study were to monitor the occurrence of Fusarium species in maize kernels in northwestern Spain to determine the risk of mycotoxin contamination and to identify environmental traits affecting the composition of the Fusarium species identified. A combination of 24 environments was evaluated. The percentage of kernels infected by F. verticillioides ranged from 33 to 99%, supporting the idea that fumonisin contamination is the main maize‐based feed and food safety concern in this area. In this region, temperature and humidity primarily affected Fusarium spp. occurrence. Warmer temperatures during the later stages of kernel development and during kernel drying increased the frequency of F. verticillioides in maize kernels, while the presence of F. subglutinans was increased by higher relative humidity during the silking stage and cooler temperatures during kernel drying.     

Post‐capture survival of whale sharks encircled in tuna purse‐seine nets: tagging and safe release methods

1. Whale shark, the world's largest fish, is believed to be particularly vulnerable owing to its biological characteristics (slow growth, late maturation, great longevity) and is listed as Vulnerable by IUCN and included in Appendix II of CITES. 2. Whale sharks are occasionally encircled in tropical tuna purse‐seine nets, throughout this global fishery. Although apparent immediate survival rates following encirclement and release have recently been assessed through scientific onboard observer programmes, a more rigorous methodology is still required for studying post‐released survival. 3. This work provides a method for applying pop‐up satellite tags and reports an enhanced release procedure for whale sharks. The first assessment of survival after release from purse‐seine nets involved six whale sharks tagged between May and September 2014 in the eastern tropical Atlantic Ocean. Five tags transmitted data: three popped up as programmed (after 30 days), while two surfaced prematurely (one after 21 and the other after 71 days (programmed to pop off after 30 and 90 days, respectively)) but showed no sign of unusual behaviour. 4. Overall, whale sharks survived at least 21 days (one at least 71 days) after release from purse‐seine nets. These observations based on five large individuals (total length > 8 m), suggest that whale sharks have a good chance of survival when released with the proposed method. 5. Additional tagging in this and other oceans, especially of juveniles which may be more sensitive to encirclement and release operations, is essential to further assess whale shark post‐release survival rates in tuna purse‐seine fisheries. Copyright © 2016 John Wiley & Sons, Ltd.     

Carbon Stocks and Fluxes in Rangelands of the Río de la Plata Basin

Grasslands are one of the most modified biomes on Earth. Land use changes had a large impact on carbon (C) stocks of grasslands. Understanding the impact of land use/land cover changes on C stocks and fluxes is critical to evaluate the potential of rangeland ecosystem as C sinks. In this article we analyze C stocks and fluxes across the environmental gradients of one of the most extensive temperate rangeland areas: the Río de la Plata Grasslands (RPG) in South America. The analysis summarizes information provided by field studies, remote sensing estimates, and modeling exercises. Average estimates of aboveground net primary production (ANPP) ranged from 240 to 316 g C· m^?2·yr^?1. Estimates of belowground NPP (BNPP) were more variable than ANPP and ranged from 264 to 568 g C· m^?2·yr^?1. Total Carbon ranged from 5 004 to 15 008 g C· m^?2. Plant biomass contribution to Total Carbon averaged 13% and varied from 9.5% to 27% among sites. The largest plant C stock corresponded to belowground biomass. Aboveground green biomass represented less than 7% of the plant C. Soil organic carbon (SOC) was concentrated in the slow and passive compartments of the organic matter. Active soil pool represented only 6.7% of the SOC. The understanding of C dynamics and stocks in the RPG grasslands is still partial and incomplete. Field estimates of ANPP and BNPP are scarce, and they are not based on a common measurement protocol. Remotely sensed techniques have the potential to generate a coherent and spatially explicit database on ANPP. However, more work is needed to improve estimates of the spatial and temporal variability of radiation use efficiency. The absence of a flux tower network restricts the ability to track seasonal changes in C uptake and to understand fine-scale controls of C dynamics.     

Tolerance of selected weed species to broadcast flaming at different growth stages

Organic producers rank weeds as the most important pests that limit their crop production. In order to optimize the use of propane flaming as a weed control tool, the objective of this study was to test tolerance of selected weed species to broadcast flaming performed at different growth stages. Six annual species, including one grass [barnyardgrass (Echinochloa crus-galli)] and five broadleaves [field bindweed (Convolvulus arvensis), kochia (Kochia scoparia), ivyleaf morning glory (Ipomoea hederacea), velvetleaf (Abutilon theophrasti) and Venice mallow (Hibiscus trionum)] were flamed at three growth stages with six doses of propane. The propane doses applied were 0, 12, 31, 50, 68 and 87 kg/ha. Flaming treatments were applied utilizing a custom built flamer mounted on a four-wheeler moving at a constant speed of 6.4 km/h. Species response to propane were described by log-logistic models based on visual injury ratings and dry matter (DM) for each weed species. Overall response to flaming varied among species, growth stage and propane dose. Broadleaf weeds were more susceptible to flaming than the grass regardless of the growth stage. A dose of 76 kg/ha of propane was needed to obtain 90% DM reduction for 7-leaf (L) barnyardgrass compared to much lower doses of 40, 49, 55, 56 and 51 kg/ha propane for 8-L bindweed, 6-L kochia, 10-L morning glory, 7-L velvetleaf and 5-L Venice mallow, respectively. Moreover, the tolerance of both grassy and broadleaf weed species to broadcast flaming increased with increase in plant size. A 90% DM reduction in velvetleaf was obtained with 42, 56 and 102 kg/ha of propane for 5-L, 7-L and 16-L stages, respectively. The tested broadleaf weed species were effectively controlled (90% DM reduction) with propane dose of 30–60 kg/ha when flamed at early growth stages (3-L to 14-L), while the same dose of propane provided only 80% DM reduction in barnyardgrass when flamed at vegetative stages (4-L to 7-L). It was not possible to obtain 90% DM reduction in barnyardgrass when flamed at flowering stage with the propane doses tested in this experiment.     

Prediction of post-thinning stem volume in slash pine stands by means of state and transition models

New Forests - Predicting growth and production is the key to effective forest management, especially in those stands where silvicultural treatments are more intensive, such as silvopastoral...     

Molecular evolution of tomato black ring virus and de novo generation of a new type of defective RNAs during long-term passaging in different hosts

Tomato black ring virus (TBRV) is a worldwide-distributed RNA virus infecting a wide range of different host plants, including crop species, trees, shrubs, and weeds. Here, we investigated the molecular evolution of TBRV and its adaptability to different plant species. The TBRV-Pi isolate was used to generate five independent evolution lineages serially passaged in either quinoa, tobacco, or tomato plants. After 15 passages, the genetic variability present in all the lineages was characterized for the movement (MP) and coat (CP) coding cistrons. We addressed two main questions: to what extent does the amount of genetic variability in the TBRV genome depend on the host species, and are there host species-specific adaptive mutations? Overall, 201 different nucleotide substitutions emerged during the evolution experiment, with some of them appearing multiple times in different lineages; two of them (one in CP and one in MP) were unique for a particular host plant. We have shown that the degree of genetic differentiation depends on the host species in which the virus evolved, and that positive selection is operating upon certain residues, particularly in CP. Moreover, we have characterized new types of defective RNAs that arose during the TBRV-Pi evolution in tobacco. Furthermore, this is the first report of a defective RNA from the RNA2 of TBRV.     

MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica

During an experimental infection of sheep with Fasciola hepatica or F. gigantica, MM3-SERO and MM3-COPRO ELISA tests were applied to compare the kinetics of antibody production and coproantigen release between the 2nd and 32nd week post-infection (wpi). The Kato-Katz technique was used to measure the kinetics of egg shedding by both Fasciola species (eggs per gram of feces, epg). The kinetics of IgG antibodies for all sheep infected with F. hepatica and F. gigantica followed a similar pattern. Optical density (OD) increased rapidly between the 4th until the 12th wpi, when the highest values were reached and then decreased slowly until the 32nd wpi. Coproantigen levels increased above the cut-off value between 6 and 9 wpi in the F. hepatica group, and between 9 and 11 wpi in the F. gigantica group. The comparison between coproantigen levels and epg indicated that F. hepatica-infected sheep had detectable amounts of coproantigens 4–7 weeks before patency (egg shedding), while F. gigantica-infected sheep had detectable amounts of coproantigens 3–6 weeks before patency. When comparing the kinetics of coproantigen release vs the kinetics of epg, a similar pattern emerged, but with a two-week time-lag in epg, for both F. hepatica and F. gigantica infections. The amount of coproantigen release by each adult was not burden dependent for F. hepatica infection (burden of 33–66 adults), while it was for F. gigantica infection (burden of 17–69 adults). The results demonstrate the usefulness of the MM3-SERO and MM3-COPRO ELISAs as tools for the diagnosis of early as well as long-term fascioliasis infections, and suggest that they can potentially be applied to human fascioliasis even in countries where F. hepatica and F. gigantica co-exist. These tests can be employed not only in the diagnosis, but also in studies on epidemiology as well as pathogenesis and treatment in animals and humans since they allow post-treatment infection monitoring.     

Regulated deficit irrigation in green bean and watermelon greenhouse crops

Mediterranean greenhouse growers of watermelon and green bean crops tend to reduce slightly the soil water availability during the flowering phase to enhance the fruit number and yield, but without measuring the soil or the plant water status. This deficit irrigation strategy (RDI) was studied on two representative growth cycles of green bean and one of watermelon. In each case, a well-watered crop acted as control.In the three well-watered vegetable crops, soil water matric potential (SMP) values were between −20 and −30 kPa throughout most of the respective growth cycles. These values avoid water deficits in Mediterranean greenhouse vegetable crops. The watermelon under RDI presented similar SMP to the well-watered crop, except during the flowering period when it reached values of −50 to −60 kPa, which are similar to, or slightly lower than, those recommended to prevent water deficits for cucurbitaceae crops. The autumn–winter and spring cycles of green bean under RDI presented progressively lower SMP values from the vegetative phase to the first fruit setting than the well-watered crops, reaching minimum SMP values of around −55 kPa for the autumn–winter cycle and of −75 kPa for the spring one. These minimum SMP values are similar for the autumn–winter cycle and lower for the spring cycle than those recommended to avoid water deficits in green bean crops grown in medium-fine textured soils. Overall, mild water deficits during flowering of watermelon and green bean crops grown in Mediterranean greenhouses did not improve the final fruit number or yield. In the two spring cycles (watermelon and green bean) the RDI strategy reduced the aboveground biomass and yield, whereas in the autumn–winter green bean cycle the RDI strategy reduced the vegetative biomass but did not affect yield. SMP threshold values can, however, be used by growers as a tool for controlling the equilibrium between the vegetative and reproductive growth of greenhouse soil-grown crops.