冻害后不同药剂处理对黔芋1号各性状的影响

通过对自然霜冻发生后的黔芋1号叶片表面直接喷施不同药剂,以期筛选出减轻马铃薯冻害、提高其产量的药剂.结果表明,喷施甲基托布津加磷酸二氢钾对冻害后的马铃薯产量有显著提高,是一种较为适用的灾后补救措施.     

半滑舌鳎含sox9基因BAC克隆的筛选及BAC-FISH定位

以本实验室构建的半滑舌鳎BAC文库为基础,通过对其进行有序混合,构建了两步PCR筛选体系;对性别决定相关的sox9基因的序列设计特异性引物,筛选获得阳性单一BAC克隆。利用BAC-FISH技术将包含sox9基因的BAC克隆定位在半滑舌鳎染色体上。结果显示,sox9基因在雌、雄半滑舌鳎的一对常染色体的长臂上分别存在两个杂交信号位点,信号稳定且特异。研究证实了该文库筛选体系的有效性;首次实现了对性别相关的sox9基因在半滑舌鳎染色体上的定位,其结果为揭示sox9基因参与鱼类性别控制的机制提供了重要基础。     

高产简化抗虫棉新品种沧198的选育研究

针对抗虫棉常规株型精细整枝、早衰严重,且整枝繁、工序多、劳动效率低的问题,依据叶枝的生育特点,创新了扩行缩株、非化控、叶枝早摘心、1株多茎育种新技术;采用多亲本杂交、病圃连续选择、株型定向培育方法,育成简化高产抗虫棉新品种沧198。该品种在河北省区域试验中,采用常规株型种植,籽棉增产10．8％～15,8％;采用多茎栽培,节省整枝用工70％,籽棉产量达3871．7～4900．5kg／hm^2,增产12．4％～22．3％,且纤维品质优良,抗逆性增强。确定的配套栽培技术简便易行,可操作性强。     

湖南省兰花产业发展现状及对策

分析当前国内外兰花发展趋势,结合湖南省兰花产业发展实际,探讨湖南省发展兰花产业的对策.     

不同油用亚麻品种的组织培养与再生体系研究

本试验主要对4个油用亚麻品种进行组织培养与再生体系建立的条件探索,比较不同基因型的组织培养能力的差异,同时可以为育种选择提供理想的材料.本研究以4种比较典型的油用亚麻品系--04-93-Ⅱ-5、03-LH-88、04-94-S-266、05-J-17为材料,研究油用亚麻不同基因型的植株组培再生能力,对4个品系的子叶及下胚轴均能诱导出愈伤组织,胚轴的诱导和再生效果普遍好于子叶.出愈率最高的品系是04-94-S-266,为72%.其再生苗率也是最高的,为48.6%.通过对愈伤组织的生长速率进行分析,说明前期经过暗处理的材料在后期生长速率较快.建立1套高效的油用亚麻植株再生体系,可为其他油用亚麻品种生物技术育种工作提供可借鉴的经验.     

民办高校大学生学习倦怠现象对策研究

学习倦怠现象在大学生中越来越普遍,且成为高校学生管理与教育方面关注的焦点问题之一。通过对辽宁5所民办高校（民办学院）1300名大学生调查发现,32．85％的民办大学生存在中度及以上学习倦怠现象,不同年级大学生学习倦怠水平存在显著差异,呈现倒“U”型发展趋势：一年级学生学习倦怠感最弱,二、三年级学生学习倦怠水平最高,四年级学生学习倦怠现象有所好转。针对研究结果,提出以下应对策略：加强就业指导,提高社会实践能力;重视专业设置,重申学习意义;注重学风建设,完善教学管理;激发学习兴趣,优化校园文化氛围;重视心理教育,健全学生人格。     

Purification and Trypsin Inhibitor Activity of a Sporamin B from Sweet Potato （Ipomoea batatas Lam. 55-2）

Sporamin is a soluble protein in sweet potato, and falls into two distinct homology groups, subfamilies A and B. In this research, a sporamin B was purified and its amino acid sequences, trypsin inhibitor activity （Ti activity） were analyzed. This sporamin B was isolated from sweet potato tubers [Ipomoea batatas （L.） Lam cv. 55-2] through extraction of the water-soluble fraction, dialysis, ultrafiltration and ion-exchange chromatography. Homology determined by polyacrylamide gel electrophoresis showed that mainly one bond appeared in gel after being reduced by SDS （sodium dodecyl sulfate）, or by SDS and 2-mercaptoethanol, or in native situation. By comparing the data of the polypeptide mass Matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry with those of the mass of the theoretical amino acid sequences from NCBI protein database, it was revealed that it was Q40091｜Q40091_IPOBA, sweet potato sporamin B - Ipomoea Batatas （sweet potato） （Batate）. The sequence coverage was 70.6%. N-terminal sequence was SETPV （Ser-Glu-Thr-Pro-Val）. There is a linear relationship between trypsin inhibitor activity （Ti activity） and amounts of this sporamin B （3-18 μg mL-1）. The equation of linear regression was y = 2.5809x ＋ 17.049 （r2 = 0.9966）. There was a curvilinear relationship between Ti activity and amounts of this sporamin B （21-150 μg mL-1）. The equation of curvilinear regression is y = 14.417ln（x） ＋ 23.26 （r2 = 0.9924）. The concentration of sporamin B with Ti activity after heating at 40°C may induce part denature of this sporamin B, and there was no statistic difference after heating at 40, 50, 60°C for 20 min. Heat treatment at more than 90°C leads to a dramatic decrease of trypsin inhibitor efficiency. The results suggested that Q40091｜Q40091_IPOBA was the major sporamin B in sweet potato tubers [Ipomoea Batatas （L.） Lam cv. 55-2], which had strong Ti activity, and was stable to both thermal and DTT （DL-dithiothreitol） relatively.     

菲律宾蛤仔土池中间育成的研究

2002~2003年在约80 hm2土池进行了菲律宾蛤仔中间育成试验。试验结果表明,在适时肥水、追肥、清池,水温20~25℃的条件下,放养密度3.0×104~5.0×104枚/m2,放养规格>1.5 mm,平均生长速度>80μm/d,最高可达250μm/d,成活率>80%;不适时肥水、追肥、清池,放养规格又偏小(壳长<0.4 mm)条件下,平均生长速度为26.6μm/d,成活率只有30%。表明放养规格、肥水、追肥、清池及适时疏苗是影响中间育成效果的主要因素。     

城乡一体化建设中存在的认识误区与对策

就城乡一体化建设中存在的6个认识误区提出了对策与建议。     

Context Model Based on 3D Wavelet Coefficients

Based on by typical coding schemes such as EZW,SPIHT, EBCOT,3D-IEZW,3D-SPIHT and 3D-ESCOT,a context model(3D-OWNER) of wavelet coefficients is proposed,which makes use of partial order,self-similarity between subbands of wavelet coefficients and the correlation of neighboring wavelet coefficients.Experimental results show that the model can achieve higher PSNR than 3D-ESCOT.