同一GAP产地鱼腥草株间基因多态性研究

目的揭示同一CAP产地鱼腥草株间的基因多态性规律,为中药稳态提取工艺中最小投料量研兄莫定基础〔方法对同一CAP产地46株鱼腥草进行ISSR和RAPD分子标记,应用NTSYS2PC软件进行聚类分析〔结果将筛选出的9条ISSR引物及8条RAPD引物扩增后分别得到134和101条带,其中呈多态性的条带数为115,72条,多态比率为85.80Ic,71.30Ic,平均遗传相似系数为0.678 6,0.694 7结论两种分子标记结果均揭示了同一CAP产地鱼腥草株间基因多态性规律,阐明了引起同一CAP产地鱼腥草株间质量不稳定的内在原因,为中药材质量稳定性控制和中药稳态提取工艺中最小投料量研兄提供实验依据、     

EFFECTS OF ANESTHETIC PROTOCOL ON NORMAL CANINE BRAIN UPTAKE OF 18F‐FDG ASSESSED BY PET/CT

The purpose of this study was to assess the effects of four anesthetic protocols on normal canine brain uptake of 2‐deoxy‐2‐[¹⁸F]fluoro‐d ‐glucose (FDG) using positron emission tomography/computed tomography (PET/CT). Five clinically normal beagle dogs were anesthetized with (1) propofol/isoflurane, (2) medetomidine/pentobarbital, (3) xylazine/ketamine, and (4) medetomidine/tiletamine–zolazepam in a randomized cross‐over design. The standard uptake value (SUV) of FDG was obtained in the frontal, parietal, temporal and occipital lobes, cerebellum, brainstem and whole brain, and compared within and between anesthetic protocols using the Friedman test with significance set at P<0.05. Significant differences in SUVs were observed in various part of the brain associated with each anesthetic protocol. The SUV for the frontal and occipital lobes was significantly higher than in the brainstem in all dogs. Dogs receiving medetomidine/tiletamine–zolazepam also had significantly higher whole brain SUVs than the propofol/isoflurane group. We concluded that each anesthetic protocol exerted a different regional brain glucose uptake pattern. As a result, when comparing brain glucose uptake using PET/CT, one should consider the effects of anesthetic protocols on different regions of the glucose uptake in the dog's brain.     

IMAGING DIAGNOSIS—FDG‐PET/CT OF A CANINE SPLENIC PLASMA CELL TUMOR

An 8‐year‐old Shih Tzu developed abdominal pain and hyperglobulinemia. A round splenic mass was noted radiographically and sonographically. The patient was evaluated by fluorodeoxyglucose positron emission tomography coupled with computed tomography (FDG‐PET/CT). There was no evidence of metastasis or bone marrow involvement on PET/CT images. The standardized uptake value (SUV) of the splenic mass was increased over the reference range (SUV=4.83). The patient was diagnosed as splenic extramedullary plasmacytoma through immunohistopathologic study. After the splenectomy, the globulin level normalized and the patient is alive without complications.     

Trade Reorientation and Its Effects on Regional Port Systems: The Korea‐China Link along the Yellow Sea Rim

ABSTRACT Owing to its competitive labor costs, its open‐market policy, and a substantial amount of capital investments, China has become a global manufacturing pole and an export‐based economy replicating the conventional Asian model but on a much wider scale. This is creating acute competition on other Asian export‐based economies such as Korea that have to adapt to the “China effect.” Consequently, many Korean manufacturing companies have repositioned their capital and equipment in China to enlarge their market potential as well as to reduce their production costs. Because Korea is adjacent to China—both are sharing the Yellow Sea Rim—this shift is creating a unique geographical dimension with a high level of functional integration of Sino‐Korean manufacturing supply chains. This transition has also brought substantial changes in the regional logistic network by organizing new flows of raw materials, parts, and final products, most of them along the Yellow Sea Rim. New logistic flows have given substantial influences on regional port competition by creating diverse links. These changes are bringing a reorientation of the regional maritime industry and of the port system.     

Evaluation of an erosion-sediment transport model for a hillslope using laboratory flume data

Climate change can escalate rainfall intensity and cause further increase in sediment transport in arid lands which in turn can adversely affect water quality. Hence, there is a strong need to predict the fate of sediments in order to provide measures for sound erosion control and water quality management. The presence of microtopography on hillslopes influences processes of runoff generation and erosion, which should be taken into account to achieve more accurate modelling results. This study presents a physically based mathematical model for erosion and sediment transport coupled to one-dimensional overland flow equations that simulate rainfall-runoff generation on the rill and interrill areas of a bare hillslope. Modelling effort at such a fine resolution considering the flow connection between interrill areas and rills is rarely verified. The developed model was applied on a set of data gathered from an experimental setup where a 650 cm×136 cm erosion flume was pre-formed with a longitudinal rill and interrill having a plane geometry and was equipped with a rainfall simulator that reproduces natural rainfall characteristics. The flume can be given both longitudinal and lateral slope directions. For calibration and validation, the model was applied on the experimental results obtained from the setup of the flume having 5% lateral and 10% longitudinal slope directions under rainfall intensities of 105 and 45 mm/h, respectively. Calibration showed that the model was able to produce good results based on the R2(0.84) and NSE(0.80) values. The model performance was further tested through validation which also produced good statistics(R2=0.83, NSE=0.72). Results in terms of the sedigraphs, cumulative mass curves and performance statistics suggest that the model can be a useful and an important step towards verifying and improving mathematical models of erosion and sediment transport.     

Allelopathic potential of K21, selected as a promising allelopathic rice

The agronomic characteristics of a newly bred potent allelopathic rice, K21, including morphological appearance, resembled its female parent, Dongjinbyeo, except for 8 days' earlier heading and 9 cm shorter plant height. The water extract of K21 showed a greater inhibitory effect on the shoot and root growth of barnyardgrass than its female parent, but less than the male parent, Kouketsumochi. At the highest extract concentration of 5.0%, the inhibitory effect of K21 on the shoot growth of barnyardgrass was ≈ 64%, similar to its male parent. The dry weight of the barnyardgrass shoot was reduced by 13.0% when grown with K21 and 30.2% when grown with its male parent. There was no inhibitory effect on the shoot dry weight of barnyardgrass when grown with the female parent. The activity of cinnamic acid 4-hydroxylase in K21 was ≈ 1.8-fold higher than that of the female parent, but significantly lower than that of the male parent. The ρ-coumaric acid content in K21 was ≈ 60% higher than that of the female parent, but it was about half of the male parent. The total amount of phenolic compounds in K21 was ≈ 11.5% higher than in the female parent, but about half of the male parent. The results obtained suggest that the newly bred K21 contains desirable allelopathic traits derived from the male parent, Kouketsumochi, and acceptable agronomic traits from its female parent, Dongjinbyeo.     

Effects of corn dried distiller's grains with solubles and enzyme premix supplements on growth performance,carcass characteristics and meat quality parameters in finishing pigs

The objective of the present study was to investigate the effects of corn dried distiller's grains with solubles (DDGS) and enzyme premix (mannanase + phytase) supplementation on the growth performance, carcass and meat quality parameters in finishing pigs. Sixty hybrid pigs (L × LW × D) with initial weight of 63.92 ± 1.50 kg were used in a 3 × 2 factorial design with main effects of DDGS levels (0, 10 and 20%) and enzyme premix levels (0% vs. 0.14%). Average daily gain (ADG, P < 0.01) and average daily feed intake (ADFI, P < 0.05) were decreased due to an increased level of DDGS additive while the feed conversion ratio was improved (P < 0.05) by adding enzyme premix. The diet cost/gain (won/kg) was saved (P < 0.01) due to an increased level of DDGS additive. There were no significant differences in carcass characteristics and meat quality parameters of Longissimus dorsi muscle by DDGS level and enzyme premix. Palmitoleic acid, oleic acid and monounsaturated fatty acid (MUFA) decreased (P < 0.05) according to DDGS level. The results indicate that DDGS may be used in feeds for finishing pig as a replacement of corn and soybean meal without affecting their carcass characteristics and meat quality.