霜桑叶与野生植物资源组配保健茶方剂探析

为提升霜桑叶利用价值,拓宽桑叶应用领域,以承德当地选择几种常见野生菜用及药用植物为研究对象,根据其不同营养成分及功效分别与霜桑叶配伍,研制出具有预防感冒、清热解毒、降低血糖血压等不同功效的复方保健茶品。     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

2012年春季塔克拉玛干沙漠腹地沙尘颗粒的组成研究

为研究塔克拉玛干沙漠腹地沙尘颗粒的微观形貌及其矿物、元素组成,应用扫描电镜能谱技术(SEM/EDX)对塔克拉玛干沙漠腹地塔中观测站2012年5月采集的PM10样品进行了识别和分析。统计622个单颗粒高分辨电镜图像及能谱的结果表明:塔克拉玛干沙漠大气PM10主要来源于地壳矿物。其中,矿物组成主要是石英、粘土矿物、方解石、岩盐,特别是岩盐含量高达14.5%,石英、方解石、石膏、岩盐单体矿物相对含量较高,但大部分都以矿物集合体的形式存在,与其它人为源颗粒物的混合主要是外混合。不同天气类型下,其矿物组成差异明显,重矿物(岩盐等)主要来源于强风就地扬起或携带的较粗颗粒。     

林业应用系统本体知识模型的构建方法研究

总结了林业应用系统本体构建的基本方法,介绍了本体知识模型的构建思路,以期为本体技术的推广应用提供参考。     

基于径向基神经网络的非寿险公司财务预警研究

选取我国保险行业中40家非寿险公司2006~2008年的混合非平衡面板数据作为研究样本，采用径向基神经网络模型从偿付能力、盈利能力、成长能力三个角度对非寿险公司财务预警进行研究。实证表明，此模型可以为管理层的财务预警提供更有有效的信息，并且在我国保险业具有样本量小，有效数据少的情况下，有着良好的运用前景。     

Study on Expression and Preliminary Immunocompetence of CIC Protein Containing Molecular Adjuvants

To express CTB-IsdBid-Clfais(CIC) protein and evaluate its immunogenicity, CTB (as a molecular adjuvant) could be tandem linked with IsdBid-Clfais gene by the overlapping PCR method, then CTB-IsdBid-Clfais(CIC) was inserted into pET-32a(+) vector to construct recombinant plasmids pET-32a(+)-CTB-IsdBid-Clfais. The recombinant plasmids pET-32a(+)-CTB-IsdBid-Clfais were transformed into Escherichia coli (E.coli) BL21 to express the CTB-IsdBid-Clfa(CIC) protein, the CIC expression protein and its immune activity were detected by Western blotting and ELISA,respectively. The results showed that the length of CIC gene were 2 072 bp, and CIC was correctly inserted into the pET-32a(+) plasmids, the pET-32a(+)-CTB-IsdBid-Clfais recombinant plasmids were successfully constructed. Western blotting result confirmed that the molecular weight of CIC proteins was 95.9 ku, which were correctly expressed by E.coli BL21 with pET-32a (+)-CTB-IsdBid-Clfais plasmids. ELISA results showed that there was no significant difference among the CIC, IsdBid and Clfais protein groups (P>0.05), and there was extremely significant difference between CIC and BSA protein groups (P<0.01). In conclusion, the pET-32a(+)-CTB-IsdBid-Clfais recombinant plasmids were successfully constructed, CIC proteins were correctly expressed, and were able to react with serum from mice immunized with IsdBid and Clfais,respectively,therefore, CIC proteins had strong immune activity.     

细菌鞭毛蛋白作为佐剂的研究进展

细菌鞭毛蛋白作为Toll样受体5(TLR5)或NOD样受体C4(NLRC4)的配体,其结构决定了其既有抗原性又具有佐剂效应。将细菌鞭毛蛋白与外源抗原混合或融合表达,已获得多种有效的候选疫苗。细菌鞭毛蛋白佐剂效应主要是通过TLR5和NLRC4信号途径协同实现的。TLR5位于细胞表面,可触发炎性因子、趋化因子和Ⅰ型干扰素的分泌,启动天然免疫应答。NLRC4是细胞质中的模式识别受体,可识别细胞质中的多种配体并诱导相应免疫应答。另外,细菌鞭毛蛋白能募集T淋巴细胞和B淋巴细胞至次级淋巴器官,促进DCs和T淋巴细胞的活化。细菌鞭毛蛋白的可塑性将会使得以细菌鞭毛蛋白为基础的疫苗成为当前和未来研究的热点。     

农业技术模式转换与对策探讨——以河南省欠发达平原农业区为例

面对当前农村劳动力转移给农业发展带来的挑战和机遇,转换农业技术模式尤为重要.采用理论分析和调查访谈相结合的方法,得出河南欠发达平原农业区农业技术模式转换必须结合本地实际,遵循诱致性轨迹,注重转换过渡模式,即以农民社会资本为纽带进行土地流转,形成土地适度渐进规模经营,以村集体、宗族等为核心发展种子、生物科技、中小型机械设...     

“中国近现代史纲要”含义辨析

“中国近现代史纲要”是中宣部、教育部“05”方案中确定的我国普通高校思想政治理论课中的四门必修课之一.从字面意义来理解“中国近现代史纲要”,我们要弄清两个概念,一是什么是“中国近现代史”,二是什么是“纲要”,弄清了这两个问题,我们就可以给中国近现代史纲要下一个完整的定义.