全文获取类型
收费全文 | 69篇 |
免费 | 0篇 |
专业分类
5篇 | |
综合类 | 1篇 |
农作物 | 1篇 |
水产渔业 | 22篇 |
畜牧兽医 | 37篇 |
植物保护 | 3篇 |
出版年
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 2篇 |
2016年 | 1篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 3篇 |
2012年 | 2篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 5篇 |
2006年 | 3篇 |
2004年 | 2篇 |
2003年 | 8篇 |
2002年 | 3篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
排序方式: 共有69条查询结果,搜索用时 15 毫秒
31.
Masahito WATANABE Mirina KOBAYASHI Masaki NAGAYA Hitomi MATSUNARI Kazuaki NAKANO Miki MAEHARA Gota HAYASHIDA Shuko TAKAYANAGI Rieko SAKAI Kazuhiro UMEYAMA Nobuyuki WATANABE Masafumi ONODERA Hiroshi NAGASHIMA 《The Journal of reproduction and development》2015,61(3):169-177
Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear
donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum. 相似文献
32.
33.
Abstract Bovine lactoferrin was evaluated for its ability to enhance resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), to infection with Vibrio anguillarum. Oral administration of lactoferrin (100 mg kg−1 ) to fish daily for 3 days prior to an intraperitoneal challenge with V. anguillarum resulted in increased survival rates, and enhanced resistance against Streptococcus sp., although to a lesser extent. In lactoferrin-treated fish, an increase in phagocytic and chemiluminescent (CL) activities of pronephros cells against V. anguillarum was observed. The phagocytic and CL activities of cells against Streptococcus sp. were also significantly increased. However, no in vitro bactericidal activities of lactoferrin against V. anguillarum or Streptococcus sp. were observed. This suggests that the lactoferrin enhanced the resistance of the rainbow trout against bacterial infection through the activation of phagocytes. 相似文献
34.
35.
D. K. SAKAI 《Journal of fish diseases》1985,8(5):397-405
Efficacy of specific antibody on serum resistance and adhesion was investigated using a pathogenic strain of Aeromonas salmonicida A-7301 (which was autoagglutinative, haemagglutinative and protease production positive), a protease-deficient, non-pathogenic mutant NTG-1 induced from A-7301 (autoagglutinative and haemagglutinative), and a non-pathogenic strain GH-7501 (non-agglutinative, non-haemagglutinative and protease positive). A-7301 could grow and produce protease extracellularly in the presence of rainbow trout anti-A-7301 serum, resulting in a considerable reduction of the antibody titre. NTG-1 similarly grew, but the titre scarcely decreased. GH-7501 could not survive in this medium. A-7301 and NTG-1 possessed a high capacity to adhere to the surface of fish monolayer cell cultures, whereas GH-7501 lacked the capacity. The capacity for adhesion was not inhibited by the antibody. Although live NTG-1 cells were ineffective as a live vaccine, sockeye salmon receiving protease fraction (obtained from extracellular products of A-7301 by DEAE-cellulose column chromatography) inactivated with normal serum, suffered only a low mortality when challenged with A-7301. Thus, although the antibody specific to autoagglutinating cells showed no effects on serum resistance and adhesion, which are involved in the infectivity of this pathogen, the possibility of protease as an effective protective antigen was demonstrated. 相似文献
36.
Saki INOUE Kayoko YONEMARU Tokuma YANAI Hiroki SAKAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):225-228
A 5-year-old male ferret presented with an enlarged canalicular testis in the left inguinal region. Microscopically, the enlarged testis consisted of a diffuse intimately admixed proliferation of c-kit-positive germ cell-like and Wilms tumor-1 protein-positive Sertoli cell-like components, but no Call-Exner body was detected. In addition, the compact proliferation of steroidogenic acute regulatory protein-intense positive interstitial cells was identified in a separate peripheral area of the mass. Based on histopathological and immunohistochemical findings, the tumor was diagnosed as a mixed germ cell-sex cord-stromal tumor with a concurrent interstitial cell tumor. 相似文献
37.
Hirotatsu MURANO Takashi OTANI Tomoyuki MAKINO Nobuyasu SEIKE Mizuki SAKAI 《Soil Science and Plant Nutrition》2009,55(2):325-332
The use of heptachlor, a cyclodiene-type insecticide, has been banned since the 1970s because of its carcinogenic potential. However, its metabolite, heptachlor exo -epoxide (HEPX), has still been detected in the fruit of cucurbits produced in some areas. It is important to reduce the daily intake of HEPX contained in food. To address this issue, the effects of carbonaceous adsorbents on the uptake of HEPX from the soil by Cucurbita maxima Dutch. (winter squash) were investigated. Amorphous organic carbons, such as peat moss and wood chips, did not affect the concentration of HEPX in the soil solution or the amount present in the shoots. In contrast, relatively condensed carbon, such as activated carbons, decreased the concentration of HEPX in the soil solution and in the shoots. The uptake amount in the shoot was closely correlated with the concentration of HEPX in the soil solution, which suggests that its uptake by C . maxima depends on its concentration in the soil solution. Activated carbons did not affect the growth of C . maxima . Therefore, the application of activated carbon to soil contaminated with HEPX appears to be an effective method of reducing the uptake of HEPX by C . maxima . 相似文献
38.
Nana TANAKA Toshitaka KANEI Munetaka IWATA Mifumi KAWABE Hiroaki KAMISHINA Mami MURAKAMI Hiroki SAKAI Sadatoshi MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1559
In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. We herein quantitatively analyzed CTL infiltration by immunohistochemical staining with granzyme B and CD3 antibodies using paraffin sections of a dog with EM associated with zonisamide. The present results indicated approximately 70% of cells at the border between the epidermis and dermis consisted of CTLs. Detection of granzyme B and CD3 using paraffin sections employed in this study can be a clinically applicable method for detecting CTLs. 相似文献
39.
Kosei SAKAI Tomoki MOTEGI James Ken CHAMBERS Kazuyuki UCHIDA Hidetaka NISHIDA Shunsuke SHIMAMURA Hiroyuki TANI Terumasa SHIMADA Masaru FURUYA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):502
A 10-month-old, intact male Toy Poodle was referred for a postural abnormality. Blood biochemical tests revealed a marked increase in plasma creatine phosphokinase (CPK) concentration. The isoenzyme test showed that 99% of serum CPK consisted of CPK-MM. Histopathological evaluation of muscle biopsy samples confirmed scattered degeneration and necrosis of myofibers. Immunohistochemistry for dystrophin showed an absence of staining in muscle cells. Based on these findings, the dog was diagnosed with dystrophin-deficient muscular dystrophy. Whole genome sequencing using genomic DNA extracted from blood revealed a single base pair insertion in exon 45 of the Duchenne muscular dystrophy (DMD) gene. This is the first report on muscular dystrophy in Toy Poodles and identified a novel mutation in the DMD gene. 相似文献