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201.
202.
Production and characterization of monoclonal antibodies against chicken lymphocyte surface antigens
T Kondo M Hattori H Kodama M Onuma T Mikami 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(1):97-103
A panel of monoclonal antibodies (mAbs) with specificity for chicken lymphocyte surface antigens was established and characterized based on their reactivities against chicken lymphoid cells and tumor cell lines on flow cytometry. Three mAbs (7-3G-2, 7-2E-8, and JB-2) reacted preferentially with thymocytes, however, none of them reacted with Marek's disease derived T lymphoblastoid cell lines. Four mAbs (6-27A-1, 4-5C-5, Lc-4, and Lc-6) reacted with spleen cells and peripheral blood leukocytes as well as thymocytes. All seven mAbs reacted with chicken embryonic thymocytes from day 12 of embryonic life onward. All mAbs showed no reactivity against bursal lymphocytes. 相似文献
203.
Shin Hiratsuka Homare Onodera Yoshitaka Kawai Tatsuya Kubo Hisashi Itoh Ryuji Wada 《Scientia Horticulturae》2001,90(3-4):255-264
Anthocyanin synthesis began in the skin of ‘Olympia’ (Vitis labruscana Bailey) grape when the berry growth reached a late stage in the second sigmoid growth-curve. During berry development, shikimate NADP oxidoreductase (SORase, EC.1.1.1.25), phenylalanine ammonia-lyase (PAL, EC.4.3.1.5), and chalcone flavanone isomerase (CHFI, EC.5.5.1.6) were investigated in the skin. These enzyme activities were high during early development of the young berry, but rapidly decreased thereafter, and were activated again when rapid anthocyanin synthesis subsequently occurred. When PAL from the young berry was analyzed by isoelectricfocusing polyacrylamide gel electrophoresis (IEF-PAGE), it showed a single activity band with a pI value of approximately 5.4, whereas PAL from the ripe berry showed two activity bands with pIs of 5.4 and 6.5. Thus, SORase, PAL, and CHFI seem to be involved in anthocyanin accumulation simultaneously, and newly synthesized isozyme of PAL may play a key role in pigment formation in ‘Olympia’ skin. The function of these enzyme activities in young berry is also discussed in this text. 相似文献
204.
205.
Fuji Shin-ichi Mochizuki Tomofumi Okuda Mitsuru Tsuda Shinya Kagiwada Satoshi Sekine Ken-Taro Ugaki Masashi Natsuaki Keiko T. Isogai Masamichi Maoka Tetsuo Takeshita Minoru Yoshikawa Nobuyuki Mise Kazuyuki Sasaya Takahide Kondo Hideki Kubota Kenji Yamaji Yasuyuki Iwanami Toru Ohshima Kazusato Kobayashi Kappei Hataya Tatsuji Sano Teruo Suzuki Nobuhiro 《Journal of General Plant Pathology》2022,88(2):105-127
Journal of General Plant Pathology - An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological... 相似文献
206.
Yamanaka T Bannai H Nemoto M Tsujimura K Kondo T Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):483-485
In 2010, the World Organisation for Animal Health recommended the inclusion of a Florida sublineage clade2 strain of equine influenza virus (H3N8), which is represented by A/equine/Richmond/1/07 (Richmond07), in equine influenza vaccines. Here, we evaluate the antigenic differences between Japanese vaccine strains and Richmond07 by performing hemagglutination inhibition (HI) assays. Ferret antiserum raised to A/equine/La Plata/93 (La Plata93), which is a Japanese vaccine strain, reacted with Richmond07 at a similar titer to La Plata93. Moreover, two hundred racehorses exhibited similar geometric mean HI antibody titers against La Plata93 and Richmond07 (73.1 and 80.8, respectively). Therefore, we can expect the antibody induced by the current Japanese vaccines to provide some protection against Richmond07-like viruses. 相似文献
207.
Yonezawa T Mogi K Li JY Sako R Manabe N Yamanouchi K Nishihara M 《The Journal of reproduction and development》2011,57(2):280-287
We previously reported that growth hormone (GH) pulses were negatively associated with neuropeptide Y (NPY) profiles in cerebrospinal fluid (CSF) of the third ventricle of Shiba goats. In addition, while most GH pulses were coincident with GH-releasing hormone (GHRH) pulses, there was no correlation between GH and somatostatin (SRIF) levels. The present study was performed to elucidate the relationship between GH pulses and these neuropeptide levels in CSF when estradiol (1.0 mg/head) was subcutaneously administered to ovariectomized goats. CSF and plasma samples were collected every 15 min for 18 h (from 6 h before to 12 h after injection). GH levels in peripheral blood and GHRH, SRIF and NPY levels in CSF were measured by radioimmunoassay. Pulse/trough characteristics and correlations were assessed by the ULTRA algorithm and cross-correlation analysis. Before estradiol was injected, significant coincidence was found between GHRH pulses and GH pulses, and negative coincidence was found between NPY troughs and GH pulses. Six to 12 h after estradiol injection, the amplitude and area under the curve (AUC) of the GH pulses were markedly increased. The duration and AUC of the GHRH pulses in the CSF were also increased, and stronger synchrony of GHRH with GH was observed. In contrast, the baseline of NPY was significantly decreased, and the negative correlation between the GH pulses and NPY troughs disappeared. The parameters of SRIF troughs were not clearly changed. These observations suggest that estrogen enhances the pattern of secretion of GH in the goat via enhancement of GHRH pulses and decrease of NPY levels. 相似文献
208.
Fukushima R Tanaka R Suzuki S Hamabe R Machida N Nakao S Saida Y Takashima K Matsumoto H Koyama H Hirose H Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1287-1293
The double-chambered right ventricle (DCRV) is a rare congenital cardiac disease in dogs, and its detailed epidemiological and morphological features are not clearly understood. By investigating the profile, clinical signs, and characteristics of examination findings of eleven dogs with DCRV by means of a retrospective study, we attempted to clarify the epidemiology and morphology of the condition. The study group consisted of nine males and two females. Breeds included Pug (n=3), Miniature Dachshund (n=1), French Bull-dog (n=1), Shiba (n=1), and Retrievers (n=5). The attachment site of the anomalous muscular bundle was continuous with the cardiac apex in nine dogs, and it was attached to the right ventricle free wall in the other two dogs. In dogs with DCRV, at least one of the following conditions was present concurrently: congenital or acquired tricuspid valve regurgitation (TR), ventricular septal defect, and atrial septal defect. Also, the pressure difference between the two chambers increased over time, and progressive right-sided heart failure was observed. In summary, DCRV occurs in small breeds of dog as well as in large breeds of dog and it may be more prevalent in males. The existence of two types of DCRV in dogs was established. Dog with DCRVs will have a high incidence of concurrent cardiac abnormalities. Concurrent TR may be either congenital or acquired. DCRV is a congenital disorder, but the clinical condition progresses as the dog develops. 相似文献
209.
210.
Oil palm trunks (OPTs) are a potential source of sugars for bioethanol production, and so we determined the glucose, fructose,
and sucrose contents of hot water extracts from OPTs. Samples of OPTs were obtained from different regions of the trunk, from
trunks of palms of different ages (31-, 19-, and 15-year-old), and from fruiting and nonfruiting palms. The extractable sugar
contents of the whole OPT and the upper and lower regions of each OPT were calculated. Our results indicated that the upper
parts of OPTs should be used for bioethanol production because they yield higher concentrations of sugars than the lower parts
do. To produce a highly concentrated glucose solution for bioethanol production, OPTs should be harvested as soon as production
of palm oil has been completed and when new male flowers have appeared. If these suggestions are followed, then the estimated
ethanol concentration after fermentation is 3.2% and the estimated ethanol production per harvested area is 3.5 kl/ha. 相似文献