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排序方式: 共有618条查询结果,搜索用时 15 毫秒
101.
102.
Janira Prichula Muriel Primon-Barros Romeu C. Z. Luz Ícaro M. S. Castro Thiago G. S. Paim Maurício Tavares Rodrigo Ligabue-Braun Pedro A. dAzevedo Jeverson Frazzon Ana P. G. Frazzon Adriana Seixas Michael S. Gilmore 《Marine drugs》2021,19(6)
New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application. 相似文献
103.
104.
Rodrigo BrionesLuis Serrano Rached Ben YounesIñaki Mondragon Jalel Labidi 《Industrial Crops and Products》2011,34(1):1035-1040
Polyol production from chemical modification of date seeds has been investigated through oxypropylation and liquefaction techniques (using organic solvents in the presence of a catalyst). The obtained products were characterized using infrared spectroscopy analysis, 1H NMR, thermogravimetric analysis and other parameters such as hydroxyl number (IOH) and viscosity. Results showed that 93% of the solid substance was converted into polyol in the oxypropylation reaction at the date seeds/propylene oxide ratio of 0.25 and 10% potassium hydroxide at 160 °C. The oxypropylated product has IOH of 779 mg KOH/g and viscosity of 6.9 Pa s. Regarding the liquefaction technique, results show that a yield of 95% was obtained at the date seeds/liquefying solvent ratio of 0.25 in 60 min of reaction at 160 °C. The liquefied product shows IOH of 336 mg KOH/g and viscosity of 0.9 Pa s. 相似文献
105.
Mónica Madrigal-Valverde Rodrigo F. Bittencourt Lindomar S. Brito Maicon P. Lents Elisiane S. Santos Anthony Valverde-Abarca 《Reproduction in domestic animals》2020,55(3):309-317
The present study aimed to evaluate differences in area, volume and testicular weight among groups related to breed and age of boar and establish associations between testicular parameters and seminal characteristics. Seminal quality has been studied using computer-assisted semen analysis (CASA) systems that reduce the subjectivity of kinematic parameters. Based on the data obtained through this system, sperm quality indexes and their derived parameters have been assessed to identify a better estimator for determining movement and velocity parameters. We also established indexes of velocity and sperm movement for boar semen. To this end, we evaluated 191 ejaculate samples from 63 boars. Differences related to the effects of season and age on quality boar semen were found (p ≤ .05) in the animal groups. Indexes were established for velocity and sperm movement based on CASA-Mot, and we proposed a sperm movement index (SMI) and sperm velocity index (SVI) with the CASA-Mot variables. Correlations were found (p < .05) among sperm indexes and CASA-Mot variables. Boar characteristics were found to be associated with some CASA-Mot variables, and the estimated SMI and SVI indexes predicted a greater variation in kinematic characteristics compared to the parameters evaluated separately using the CASA-Mot system. 相似文献
106.
A combination of plant oils promotes adequate growth of the freshwater catfish Rhamdia quelen (Quoy & Gaimard 1824) 下载免费PDF全文
Rodrigo Javier Vargas Alexandre Sachsida Garcia Sueli Regina Baggio Eduardo Cargnin‐Ferreira Débora Machado Fracalossi 《Aquaculture Research》2015,46(12):3046-3055
Essential fatty acids should be included in the diet to ensure adequate fish growth. Despite the great number of studies on fatty acid nutrition of fish, there are still several unknowns. The aim of the present study was to investigate fatty acid nutrition of jundiá, a Latin American freshwater catfish. Four diets were formulated containing (i) coconut oil (?C, negative control), (ii) coconut oil + high‐docosahexaenoic‐acid‐fish oil (+C, positive control) and coconut + sunflower + linseed oils at different ratios, producing either (iii) a diet rich in linoleic acid (LA) (HighLA) or 4) a diet low in LA (LowLA). All diets contained significant amounts of saturated fatty acids (at least 57.5% total fatty acids in HighLA) and monounsaturated fatty acids (at least 19.1% total fatty acids in ?C). Diets were fed to jundiá fingerlings (1.5 g) for 70 days; growth, body composition and liver histology were evaluated. The ?C diet, without essential fatty acids, promoted significantly lower fish growth, body fat accumulation and hepatic lipidosis. Fish fed HighLA and LowLA diets presented similar growth as fish fed +C diet. These findings suggest that diet formulations for jundiá catfish fingerlings can include only plant oils without negative effect on growth, survival, body composition, fish health or parameters of feed utilization (ingestion rate and protein utilization). 相似文献
107.
Salty stories,fresh spaces: Lessons for aquatic protected areas from marine and freshwater experiences 下载免费PDF全文
Erin K. Loury Shaara M. Ainsley Shannon D. Bower Ratana Chuenpagdee Tracy Farrell Amanda G. Guthrie Sokrith Heng Zau Lunn Abdullah Al Mamun Rodrigo Oyanedel Steve Rocliffe Suvaluck Satumanatpan Steven J. Cooke 《水产资源保护:海洋与淡水生态系统》2018,28(2):485-500
108.
Rodrigo C. Silva Virgínia B. Richini-Pereira Mariana Kikuti Pâmela M. Marson 《The Veterinary quarterly》2017,37(1):23-30
Background: Canine visceral leishmaniasis (CVL) is a worldwide parasitic zoonosis caused by Leishmania (Leishmania) infantum around the world. Canids are the definitive hosts and sand flies the intermediate hosts.Objective: To test the hypothesis that a new species-specific primers (Lch14:Lch15, targeting a multiple alignment for L. infantum kDNA minicircle) is an efficient diagnostic tool for L. infantum.Methods: The presence of L. infantum DNA was assessed in blood samples of 69 stray dogs using the conventional PCR (cPCR) and quantitative PCR (qPCR). Additional 50 lymph nodes and 50 bone marrow samples (positive and negative samples for parasitological tests) from dogs from endemic and nonendemic areas for CVL were also used. Results: L. infantum strains, and all positive lymph node and bone marrow samples for parasitological test gave positive results for cPCR and qPCR, presenting analytical sensitivity of ~100 parasite mL?1. For the blood samples, 40/69 (58%; CI 95%; 46%–69%) resulted positive for L. infantum in both tests. All positive samples were confirmed by sequencing.Conclusion: This study showed the importance of the specific detection of L. infantum based on species-specific primers by molecular techniques, highlighting the application as a confirmation method in epidemiological studies and to adopt the best control measures. 相似文献
109.
New Forests - Pinus pinea is an interesting species for its valued pine nuts. Despite the high demand for this nut, the species is mostly harvested from natural forests, because the time elapsed... 相似文献
110.
Cyanobacteria are important for global nitrogen cycle and often form complex associations referred to as cyanobacterial mats
or periphyton that are common in tropical, limestone-based wetlands. The objective of this study was to monitor the nitrogen
fixation rate using the acetylene reduction assay of these cyanobacterial mats in a tropical, unfertilized, and protected
wetland. To account for temporal and spatial variation of nitrogenase activity, we were interested in seasons in a hydrological
cycle (dry, rains, and end of rains), sites with different vascular vegetation, and rates of nitrogenase activity in a 24-h
cycle. The annual average of nitrogenase activity was 22 nmol C2H4 cm−2 h−1, with a range of <6 to 35 nmol C2H4 cm−2 h−1, and the annual nitrogen fixation rate of our study site (9.0 g N m−2 year−1) is higher than similar estimates from other freshwater wetlands. There was a clear temporal pattern in nitrogenase activity
with a maximum rate occurring during the rainy season (August) and a maximum nitrogenase activity occurring between 0600 and
1200 hours. We found spatial differences in nitrogenase activity among the four sites that could be attributed to variations
in species composition within the periphyton. 相似文献