Salinity tolerance of non‐native suckermouth armoured catfish (Loricariidae: Pterygoplichthys) in south‐eastern Mexico: implications for invasion and dispersal

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Grazing land management and biodiversity in the Atlantic European heathlands: a review

Atlantic heaths are semi-natural habitats of high biodiversity interest which once covered large areas of the Atlantic Region. Nowadays these heathlands are dramatically reduced in many countries although they still cover wide areas in the north-west Iberian Peninsula, especially in the poorest and most socially marginal areas that are frequently affected by wildfires. We review the role of livestock grazing as a sustainable management strategy for heathlands in Europe. We have worked on a generalized conceptual framework for the management of a resource of nutritional and environmental value by drawing together evidence from studies of the livestock and the community ecology of grazed plants and the associated fauna. Key factors that influence grazing impact, such as type of livestock (animal species and breed) and their management are discussed. Goats thrive better than sheep, and horses than cattle, when heathland vegetation is the predominant resource available. Regardless of the type of livestock species managed, the low nutritive value of this vegetation hinders the maintenance of productive groups of suckler dams with offspring through the grazing season. The nutritional requirements of livestock can be met by adding improved pasture areas to heathlands. Under that strategy, sheep have the best productive performance and cattle the poorest. Management of mixed flocks with goats can lead to a more efficient use of vegetation, improve productivity and develop a patchier habitat which supports a richer associated fauna. Overall the results indicate that the sustainability of livestock grazing in these marginal lands will be achieved if they are managed effectively according to the available vegetation and their effects on the biodiversity.     

Preliminary pharmacokinetics of tramadol hydrochloride after administration via different routes in male and female B6 mice

Objective

1) To determine the pharmacokinetics of tramadol hydrochloride and its active metabolite, O-desmethyltramadol (M1), after administration through different routes in female and male C57Bl/6 mice; 2) to evaluate the stability of tramadol solutions; and 3) to identify a suitable dose regimen for prospective clinical analgesia in B6 mice.

Evaluation and comparison of postoperative analgesic effects of dexketoprofen and methadone in dogs

Objective

To evaluate and compare the analgesic efficacy and adverse effects of dexketoprofen and methadone using a noninferiority trial, during the first 24 postoperative hours in dogs undergoing orthopaedic surgery.

Response of juvenile astacid crayfish (Pacifastacus leniusculus) to three commercial dry diets with different protein levels during the first 6 months of intensive rearing

A 180‐day experiment was carried out under controlled conditions to compare the effects of three commercial dry diets on survival, growth and body composition of juvenile Pacifastacus leniusculus from the onset of exogenous feeding (stage 2). Protein levels in the diets were 55.5% [high‐protein content (HP) diet], 40.3% [medium‐protein content (MP)] and 33.2% [low‐protein content (LP)]. Diets were supplemented with restricted amounts of live fresh Artemia nauplii up to day 120. No significant differences in survival among diets were found along the trial, with final values around 68.5%. During the live feed supplementation, growth was similar with the three diets. At the end of the trial, weight with HP or with MP was similar (around 2.32 g) and significantly higher than with LP (1.97 g). Thus, a diet with 40% of protein can be suitable, as it allowed similar results as the diet containing 55%. Levels of protein in diets and in crayfish muscle were positively correlated (r=0.8). This study also shows that an intensive rearing up to 180 days with good survival and growth rates is possible using dry diets for finfish species supplemented with live feed.     

Molecular and Chemical Analysis of the Lipopolysaccharide from Aeromonas hydrophila Strain AH-1 (Serotype O11)

A group of virulent Aeromonas hydrophila, A. sobria, and A. veronii biovar sobria strains isolated from humans and fish have been described; these strains classified to serotype O11 are serologically related by their lipopolysaccharide (LPS) O-antigen (O-polysaccharide), and the presence of an S-layer consisting of multiple copies of a crystalline surface array protein with a molecular weight of 52 kDa in the form of a crystalline surface array which lies peripheral to the cell wall. A. hydrophila strain AH-1 is one of them. We isolated the LPS from this strain and determined the structure of the O-polysaccharide, which was similar to that previously described for another strain of serotype O11. The genetics of the O11-antigen showed the genes (wb_O11 cluster) in two sections separated by genes involved in biosynthesis and assembly of the S-layer. The O11-antigen LPS is an example of an ABC-2-transporter-dependent pathway for O-antigen heteropolysaccharide (disaccharide) assembly. The genes involved in the biosynthesis of the LPS core (waa_O11 cluster) were also identified in three different chromosome regions being nearly identical to the ones described for A. hydrophila AH-3 (serotype O34). The genetic data and preliminary chemical analysis indicated that the LPS core for strain AH-1 is identical to the one for strain AH-3.     

Comparison of the cardiopulmonary parameters after induction of anaesthesia with alphaxalone or etomidate in dogs

ObjectiveTo compare the cardiorespiratory effects and quality of induction of and recovery from anaesthesia following etomidate or alphaxalone-HPCD IV.Study designRandomized ‘blinded’ cross-over study. Twenty-four hours was allowed between phases.AnimalsEight healthy adult Beagles (four male, four female).MethodsDogs were anaesthetized with sevoflurane for instrumentation, then allowed to awake. They then received etomidate (treatment E) or alphaxalone-HPCD (treatment A) intravenously to effect. Heart rate (HR), body temperature, invasive arterial pressures (AP), systemic vascular resistance index (SVRI), stroke volume index, cardiac index (CI), contractility, respiratory rate, central venous pressure, and capnometry were obtained before anaesthetic induction (baseline), 30 seconds and 1 minute after induction, after intubation, one minute after intubation, and for every 5 minutes afterwards until the dog began to swallow and the trachea was extubated. Arterial bloods were taken for analyses before induction, after intubation and every 10 minutes thereafter. The dogs breathed room air. The quality of induction of and recovery from anaesthesia were scored categorically. Statistical analyses used anova for repeated measures, paired t-tests or Wilcoxon signed rank-test as relevant. Significance was set at p < 0.05.ResultsThe induction doses required were (mean ± SD) 2.91 ± 0.41 mg kg^?1 and 4.15 ± 0.7 mg kg^?1 for treatment E and A respectively. No significant changes in cardiovascular parameters were observed with treatment E. Treatment A resulted in statistically significant increases in HR and CI and reductions of APs and SVRI. Time to extubation was longer with treatment A (25 ± 7 minutes) than with treatment E (17 ± 4 minutes). Dogs became hypoxic with both treatments. The quality of induction and recovery were excellent with treatment A, but significantly less satisfactory with treatment E (recovery score, treatment E median 1, range 0–2; treatment A median 0, range 0–1).Conclusions and clinical relevanceAlphaxalone-HPCD caused significant tachycardia and increase in CI, and statistically (but not clinically) significant decreases in APs and SVRI. Etomidate caused no statistically significant cardiovascular changes. Quality of recovery was better with alfaxalone-HPCD. Both agents caused short-lived hypoxia, and oxygen supplementation is advisable.     

Exploring the garlic (Allium sativum) properties for fish aquaculture

Fish Physiology and Biochemistry - The aquaculture industry’s rapid growth to meet commercial demand can trigger an outbreak of infectious diseases due to high-density farming. Antibiotic...     

Analysis of hop acids and their oxidized derivatives and iso-alpha-acids in beer by capillary electrophoresis-electrospray ionization mass spectrometry

This study investigates the applicability of on-line coupling of capillary electrophoresis with electrospray ionization tandem mass spectrometry (CZE-ESI-MS) for the separation and characterization of alpha- and beta-acids and oxidized hop acids from crude extracts of different hop varieties. CZE-ESI-MS with negative-ion electrospray ionization proved to be a suitable technique for the determination of these types of natural compounds and their oxidized derivatives. The CZE parameters (pH, concentration, and buffer type) and ESI-MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure, drying gas flow rate, temperature, and compound stability) were optimized. The optimized method provides the potential for a fast qualitative determination of hop acids and their oxidation compounds. The method was also applied to the determination of iso-alpha-acids in beer.