Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F₁s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding.     

Effect of different feeds on the growth,survival and reproductive performance of zebrafish,Danio rerio (Hamilton, 1822)

Present experiment was conducted to determine the effect of different feeds with varying protein levels on the growth, survival and reproductive performance of zebrafish, Danio rerio. The control diet (T1) was wild‐collected zooplankton from local fish ponds, while test diets with 350 g kg^?1 protein (T2), 400 g kg^?1 protein (T3) and 450 g kg^?1 protein (T4) were formulated and fed to fish for a period of 210 days. The significantly (P < 0.05) highest mean weight gain and specific growth rate were observed in T1, which were similar with T3 and T4. The significantly (P < 0.05) highest number of egg production per female and relative fecundity were found in T1, followed by T4 and T3 while T2 produced lowest number of eggs. No significant (P > 0.05) differences were observed in brood survival rate, fertilization and hatching rate among the dietary treatments. The highest (P < 0.05) fry survival rate was recorded in T1, followed by T3 and T4. Thus, it is suggested that control diet i.e. mixed zooplankton exhibited better growth, reproductive performance and fry survival rate. However, diet containing 400 g kg^?1 crude protein also gave comparable results in terms of growth, survival and reproductive performance of zebrafish.     

Effect of irrigation and nitrogen application methods on input use efficiency of wheat under limited water supply in a Vertisol of Central India

Field experiments were conducted in a deep Vertisol at the Indian Institute of Soil Science, Bhopal during the years 2001–2005 to assess the effect of five different irrigation strategies through combinations of sprinkler and flood irrigation and two N application methods on yield and water use efficiency of wheat (cv WH 147). The amount of irrigation applied each year differed according to the availability of water in the water harvesting pond to simulate the actual water crisis faced by the farmers in this region during these years due to monsoon failure. Results indicated that when wheat was grown only with 8-cm irrigation at sowing or 14 cm up to the crown root initiation stage, dry sowing of wheat immediately followed by sprinkler and subsequent irrigation through flooding produced the highest yield and water and nitrogen use efficiencies. However, when 20-cm irrigation was supplied up to the flowering stage or 14-cm irrigation was supplied up to tillering stage through sprinkler in 4 and 3 splits, respectively, at critical growth stages, maximized the grain yield and water and nitrogen use efficiencies. Across the years, the crop yield and water and nitrogen use efficiencies increased with increase in water supply.     

Economic analysis of risk of gastrointestinal parasitic infection in cattle in North Eastern States of India

Gastrointestinal parasitic infection is highly prevalent in the North Eastern States (NEH) of India and accounted for significant economic losses across various livestock species. Productivity of cattle in terms of milk yield was estimated to be considerably higher (3,715, 3,590, and 3,154 L) due to strategic anthelmintic treatment as compare to control group (2,928 L). Based on the probability of occurrence of parasitic infection as well as increase in value of milk production, the possible economic gain at state level has been estimated to be Rs. 46 million, Rs. 35 million, and Rs. 14 million, depending upon the different strategic treatment. The government may take up the program to educate the cattle farmers on strategic management against parasitic infecion and simultaneously making available various anthelmintic medicines. This public responsibility of the government to minimize the risk and economic loss due to gastrointestinal parasite infection may reduce the private cost and thereby would increase the social benefits in North Eastern states of India.     

Molecular evidence supports hypervariability in Phytophthora colocasiae associated with leaf blight of taro

The oomycete Phytophthora colocasiae that causes taro leaf blight is the most devastating disease of taro and is widely distributed worldwide. Molecular and phenotypic techniques were employed for assessing and exploiting the genetic variability among four populations of P. colocasiae obtained from a fine spatial scale (multiple leaf blight lesions on single taro leaf). Phenotypic characters such as virulence, morphology and mating type showed no variation. ITS characterization revealed detectable polymorphism among isolates of P. colocasiae. The mean number of haplotypes (H), haplotype diversity (HD), nucleotide diversity (π), and nucleotide substitution rate (θ) among analyzed sequences were 6.75, 1.00, 0.069, and 0.088 respectively. High levels of inter and intra specific variation were detected by random amplified polymorphic DNA (RAPD) assays. Moderate genetic diversity (H?=?0.2651) was observed among populations of P. colocasiae. Analysis of molecular variance (AMOVA) confirmed that most of the genetic variability was confined to within a population (63.54 %). The coefficient of genetic differentiation among populations (G _ST ) was 0.2007 and estimates of gene flow (Nm) among populations was 1.991 migrants per generation. Cluster analysis using UPGMA revealed that individuals from the same population failed to cluster in one distinct group. The results of the study reveal considerable genetic diversity among and within populations of P. colocasiae obtained from fine spatial scale. The possible mechanisms and implications of this genetic variation are discussed.     

Root biomass, crop response and water-yield relationship of mustard (Brassica juncea L.) grown under combinations of irrigation and nutrient application

A 3-year study was carried out to assess the root biomass production, crop growth rate, yield attributes, canopy temperature and water-yield relationships in Indian mustard grown under combinations of irrigation and nutrient application for revealing the dynamic relationship of crop yield (Y) and seasonal evapotranspiration (ET). Three post-sowing irrigation treatments viz. no irrigation (I ₁), one irrigation at flowering (I ₂) and two irrigations one each at rosette and flowering stage (I ₃), three nutrient treatments viz. no fertilizer or manure (F ₁), 100% recommended NPK i.e., 60 kg N, 13.1 kg P and 16.6 kg K ha⁻¹ (F ₂) and 100% recommended NPK plus farmyard manure @ 10 Mg ha⁻¹ (F ₃) were tested in a split-plot design. Root biomass was significantly greater in I ₃ than I ₂ and I ₁, and in F ₃ than F ₂ and F ₁. The I ₃ × F ₃, I ₂ × F ₃ and I ₃ × F ₂ combinations maintained significantly greater crop growth rate, plant height, yield components, ET and crop yield and better plant water status in terms of canopy temperature, canopy-air temperature difference (CATD) and relative leaf water content (RLWC). Number of siliqua plant⁻¹ and seeds siliqua⁻¹ were the major contributors to the seed yield. Marginal analysis of water production function was used to establish Y–ET relationship. The elasticity of water production (E _wp) provides a means to assess relative changes in Y and ET, and gives an indication of improvement of Y due to nutrient application. The ET–Y relationships were linear with marginal water use efficiency (WUE_m) of 3.09, 4.23 and 3.95 kg ha⁻¹ mm⁻¹ in F ₁, F ₂ and F ₃, respectively, and the corresponding E _wp were 0.63, 0.71 and 0.61. This implies that the scope for improving yield and WUE with 100% NPK was little compared with 100% NPK + farmyard manure. The crop yield was highest in I ₃ × F ₃ combination, and the similar yield was obtained in I ₂ × F ₃ and I ₃ × F ₂ combinations. Application of organic manure along with 100% NPK fertilizers maintained greater crop growth rate, better water relation in plants, yield attributes and saved one post-sowing irrigation.     

Rapid diagnosis of tuberculosis in dromedary camel (Camelus dromedarius) using lateral flow assay-based kit

Accurate early antemortem diagnosis of tuberculosis in dromedary camels is difficult due to the lack of reliable diagnostic test. The present study aimed to evaluate a lateral flow assay-based kit (rapid assay kit) in tuberculosis diagnosis that employs immuno-chromatographic detection of antibodies in serum, plasma, or whole blood. In a dromedary camel herd comprising 337 animals located at Bikaner, Rajasthan, India, 50 adult weak camels (11 males and 39 females) were tested by applying a single intradermal tuberculin test (SIDT) and rapid assay kit. A total of 14 animals (2 males, 12 females) were found positive in rapid assay. In SIDT, four animals revealed a positive reaction in the neck region and seven animals in the tail base. Another male animal was found SIDT positive but negative in rapid assay; it died after 12 months. Nine rapid assay positive animals died asymptomatically in 1- to 11-month period revealing postmortem tuberculosis lesions that were confirmed by Ziehl-Neelsen staining and histopathology. No tuberculous lesion was evident in the animal found positive in SIDT alone. Results of the present study indicated that serological tests like rapid assay kit can serve as a reliable test for antemortem diagnosis of tuberculosis in dromedary camel.

     

Swietenine: A potential oral hypoglycemic from Swietenia macrophylla seed

Swietenine, a tetranortriterpenoid, was isolated from the Swietenia macrophylla seeds. The in vivo hypoglycemic activity was evaluated against neonatal-streptozotocin induced type 2 diabetic rats. Oral administration of swietenine at 25 and 50 mg/kg body weight per day to diabetic rats was found to possess significant dose dependant hypoglycemic and hypolipidemic activity in type 2 diabetic rats.     

Soil loss tolerance values for different physiographic regions of Central India

In India, a single soil loss tolerance ( T ) value of 11.2 Mg/ha is by default used for formulating land restoration strategies for all soil types, climates and vegetation covers. However, this single 'T' value does not represent a true picture of soil loss tolerance under all land uses. The objective of our study was to develop adjusted ' T ' values for Central India using soil infiltration rate, bulk density, total organic carbon, erodibility and soil pH. Soil state was defined using a quantitative model involving different soil functions which were used to convert soil parameters to a 0 to 1 scale. The normalized values were then multiplied by appropriate weighing factors based on relative importance and sensitivity analysis of each indicator. Based on overall assessment, each soil mapping unit was categorized into soil groups I, II or III. A general guideline of USDA – Natural Resource Conservation Service (USDA-NRCS) was followed to calculate soil loss tolerance for each soil group using effective soil depth. Adjusted ' T ' values for Central India ranged from 2.5 to 12.5 Mg/ha compared with a default value of 11.2 Mg/ha. Use of these values for soil mapping units will improve conservation planning and assist with planning the development of sustainable agriculture.