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51.
Proteases that reside in cellular membranes apparently wield water to hydrolyze the peptide bonds of substrates despite their water-excluding environment. Although these intramembrane proteases bear little or no sequence resemblance to classical water-soluble proteases, they have ostensibly converged on similar hydrolytic mechanisms. Identification of essential amino acid residues of these proteases suggests that they use residue combinations for catalysis in the same way as their soluble cousins. In contrast to classical proteases, however, the catalytic residues of intramembrane proteases lie within predicted hydrophobic transmembrane domains. Elucidating the biological functions of intramembrane proteases, identifying their substrates, and understanding how they hydrolyze peptide bonds within membranes will shed light on the ways these proteases regulate crucial biological processes and contribute to disease. 相似文献
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Jeana Pereira da Silva Sheila Canevese Rahal Maria Cristina Reis Castiglioni Raphael Augusto Baldissera Gonçalves Danuta Pulz Doiche Anneke Moresco Maria Jaqueline Mamprim Luiz Carlos Vulcano 《Anatomia, histologia, embryologia》2020,49(4):541-549
This study aimed to evaluate normal features of the heart and lower respiratory tract in toco toucans by means of radiography and helical computed tomography (CT) scanner. Fifteen healthy adult toco toucans (Ramphastos toco), 10 females and 5 males, average body mass of 650 g were studied. CT examination as well as right lateral and ventrodorsal radiographic examinations of the coelomic cavity were performed under chemical restraint. Heart, lungs, air sacs, trachea and syrinx were analysed. The mean values of heart length, heart width and thoracic cavity in radiographs were, respectively, 23.76 mm, 25.94 mm and 48.87 mm. In both X-rays and CT scans, the lung parenchyma had honeycomb-like pattern. The topographic areas of the anterior and posterior air sacs were visualized as dark and air-filled spaces in X-rays. On CT evaluation, the air sacs occupied a larger area in the coelomic cavity compared to X-ray. In the lateral radiographic view, the cervical part of the trachea was positioned more ventrally in the transition from cervical to thoracic regions showing a V-shaped appearance. In all CT planes was visible division of the trachea into the right and left main bronchi at the level of 3rd thoracic vertebra. The syrinx was difficult to visualize in X-rays, but on CT it was easily identified in axial slice. In conclusion, the normal features of toco toucan's heart and lower respiratory tract that were determined on X-rays and CT scans are useful to compare with sick toco toucans, as well as other bird species. 相似文献
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以9种豆科牧草为试材,在缺磷砂土上研究施用Ca(H2PO4)2后对其产酸量的影响.结果表明:Lupinus albus,Lupinus lutens和Biserrula pelecinus施磷需求量比Cicer aritimum,Vicia faba,Medicago polgmorpha,Ornithopus sativus和Trifolium subterraneum低,而Lupinus angustifolius需求量最高.当增施磷肥,使其达到中度缺磷水平时,比酸度有所增加,但进一步增施磷肥则使植株产酸量减少,在几种植物中,C.arietinum产酸量最高,B.pelecinus最低.比酸度与过量阳离子、灰碱、钙和镁等浓度有关.在不考虑施磷和植物品种的情况下,植物产酸总量与植株地上部分过量阳离子总量恰好一致;在施磷量不同的情况下,豆科植物产酸量主要由其吸收阳离子和阴离子的差异决定. 相似文献
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Practical diets with essential oils of plants activate the complement system and alter the intestinal morphology of Nile tilapia 下载免费PDF全文
Gustavo M. R. Valladão Sílvia U. Gallani Gabriela Pala Raphael B. Jesus Suzana Kotzent Jaqueline C. Costa Thiago F. A. Silva Fabiana Pilarski 《Aquaculture Research》2017,48(11):5640-5649
The effect of the essential oils (EOs) of peppermint, Mentha piperita L., and tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel, on the haematological, biochemical, and immunological parameters and intestinal morphology of Nile tilapia, Oreochromis niloticus L., was evaluated. Fish (58.09 ± 5.87 g) were fed 100 mg/kg and 250 mg/kg of each EO and sampled on days 7, 14, 30 and 60 after starting supplementation. The haematological and biochemical parameters were not altered by the supplementation of EOs compared to the control (p > .05). With regard to the immunological parameters, the activation of the complement system of fish fed 250 mg/kg peppermint and 100 mg/kg and 250 mg/kg tea tree EOs were significantly higher compared to the control after 60 days of feeding (p < .05). The complement system plays an essential role in innate immunity and contributes significantly to the acquired immune response; thus, its activation through supplementation with EOs is promising for the formulation of nutritional additives in aquaculture. Regarding intestinal morphology, fish fed 250 mg/kg tea tree EO presented higher villus size compared to all other groups (p < .05), which represents a healthier gut. These fish present a larger intestinal surface, which can result in better absorption and utilization of the nutrients. Based on the responses found in this study, both EOs were considered promising for the formulation of feed additives for Nile tilapia. 相似文献
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Evaluating bath treatment effectiveness in the control of sea lice burdens on Atlantic salmon in New Brunswick,Canada 下载免费PDF全文
R Gautam R Vanderstichel A S Boerlage C W Revie K L Hammell 《Journal of fish diseases》2017,40(7):895-905
The use of medicinal bath treatment for sea lice is becoming more common, due to increasing resistance to in‐feed treatments with emamectin benzoate. Common treatment modalities in New Brunswick, Canada, include Salmosan administered by tarpaulin or wellboat, and Paramove administered by wellboat. In this study, we assessed the effectiveness of these treatment modalities in the field between 2010 and 2015 using a web‐based sea lice data management system (Fish‐iTrends©). Effectiveness was evaluated for adult female (AF) and for pre‐adult and adult male (PAAM) life stages separately. We also investigated the impact of variability in pretreatment lead and post‐treatment lag time on effectiveness measures. There were 1185 treatment events at 57 farms that uniquely matched our pre‐ and post‐treatment count criteria. The effectiveness of treatment modality was significantly influenced by season, pretreatment level of sea lice and by lead and lag times. In summer, Salmosan administered by tarpaulin had the greatest effectiveness on both AF and PAAM, when pretreatment levels were above 10 sea lice; whereas in autumn, the performance of treatment modalities varied significantly, depending on the pretreatment levels for the life stages. Ignoring the lead or lag time effect generally resulted in an underestimation of treatment effectiveness. 相似文献
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ELASTOGRAPHIC EVALUATION OF NATURALLY OCCURING TENDON AND LIGAMENT INJURIES OF THE EQUINE DISTAL LIMB 下载免费PDF全文
Meghann Lustgarten W. Rich Redding Raphael Labens Weston Davis Thomas M. Daniel Emily Griffith Gabriela S. Seiler 《Veterinary radiology & ultrasound》2015,56(6):670-679
Compression elastography is an ultrasonographic technique that estimates tissue strain and may have utility in diagnosing and monitoring soft tissue injuries in the equine athlete. Recently, elastography has been proven to be a feasible and repeatable imaging modality for evaluating normal tendons and ligaments of the equine distal forelimb. The purposes of this prospective study were to investigate the ability of elastography to detect spontaneously occurring lesions of equine tendons and ligaments diagnosed with magnetic resonance imaging (MRI) and gray‐scale ultrasound (US) and to characterize the differences in the elastographic appearance of acute vs. chronic injuries. Fifty seven horses with a total of 65 lesions were evaluated. Images were assessed quantitatively and qualitatively. Acute lesions were found to be significantly softer (P < 0.0001) than chronic lesions (P < 0.0001) and the stiffness of lesions increased with progression of healing (P = 0.0138). A negative correlation between lesion hypoechogenicity and softness was appreciated with more hypoechoic lesions appearing softer (P = 0.0087) and more hyperechoic regions harder (P = 0.0002). A similar finding occurred with increased signal intensity on short tau inversion recovery (STIR) and proton density (PD) MRI sequences correlating with increased softness on elastography (P = 0.0164). Using US and MRI as references, commonly encountered soft tissue injuries of the equine distal limb could be detected with elastography. However, elastography was limited for detecting small, proximal injuries of the hindlimb proximal suspensory ligament. Elastographic evaluation of equine tendons and ligaments may allow better characterization of lesion chronicity and severity, and sequential examinations may optimize lesion management, rehabilitation, and return to training. 相似文献
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Karsak M Gaffal E Date R Wang-Eckhardt L Rehnelt J Petrosino S Starowicz K Steuder R Schlicker E Cravatt B Mechoulam R Buettner R Werner S Di Marzo V Tüting T Zimmer A 《Science (New York, N.Y.)》2007,316(5830):1494-1497
Allergic contact dermatitis affects about 5% of men and 11% of women in industrialized countries and is one of the leading causes for occupational diseases. In an animal model for cutaneous contact hypersensitivity, we show that mice lacking both known cannabinoid receptors display exacerbated allergic inflammation. In contrast, fatty acid amide hydrolase-deficient mice, which have increased levels of the endocannabinoid anandamide, displayed reduced allergic responses in the skin. Cannabinoid receptor antagonists exacerbated allergic inflammation, whereas receptor agonists attenuated inflammation. These results demonstrate a protective role of the endocannabinoid system in contact allergy in the skin and suggest a target for therapeutic intervention. 相似文献