Genotypic variation for grain and stover yield of dryland (rabi) sorghum in India: 1. Magnitude of genotype × environment interactions

Grain and stover yield are key traits for the improvement of rabi sorghum varieties and hybrids in India. Large genotype-by-environment interactions (GEI) were identified for both grain and stover yield based on 10 years’ data available from the All India Coordinated Sorghum Improvement Program (AICSIP) experiments conducted from 1986/87 to 1996/97. From the estimates of the genotypic, GEI and residual components of variance for the four traits, a multi-environment testing strategy based on 15–20 locations, 2–3 years, and two replicates per trial would be required to achieve estimates of repeatability of approximately 0.70 for grain yield, 0.45 for stover yield, 0.70 for flowering time, and 0.85 for plant height.     

Assessment of rice hydraulic loading impacts on groundwater and salinity levels

This paper describes the impact of rice hydraulic loading (percentage area under rice crop) on groundwater levels and salinity in the Murrumbidgee irrigation area (MIA), Australia using a MODFLOW-based modelling approach. The model simulations show that the groundwater levels will be in equilibrium after a fall of approximately 1 m under most of the areas, however, the groundwater salinity levels will rise by more than 1,000 μs/cm in most parts of irrigation area. If the rice growing area is reduced by 50 and 75%, there can be a net decline in groundwater levels during the first 2 years and then a new quasi-equilibrium will be established. To downscale these results at the farm level, SWAGMAN Farm model in conjunction with groundwater outflow rates obtained from a three-dimensional MODFLOW model was applied for determining net recharge rates under rice for different areas within the MIA. The highest net recharge during 2005–2006 season was 0.84 ML/ha (84 mm) in parts of the irrigation system, whereas the average net recharge due to rice hydraulic loading for the whole MIA during 2005–2006 season was estimated as 0.34 ML/ha (34 mm).     

Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.

Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding.It is desirable to generate polyploid rapeseed(Brassica napus L.)germplasm with varying flowering time controlled by a few genes.In the present study,Bna SVP,a rapeseed homolog of the Arabidopsis SVP(Short Vegetative Phase)gene,was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool.A single construct targeting multiple sites was successfully applied to precisely mutate four copies of Bna SVP.The induced mutations in these copies were stably transmitted to subsequent generations.Homozygous mutants with loss-of-function alleles and free transgenic elements were generated across the four Bna SVP homologs.All mutant T₁lines tested in two environments(summer and winter growing seasons)showed early-flowering phenotypes.The decrease in flowering time was correlated with the number of mutated Bna SVP alleles.The quadruple mutants showed the shortest flowering time,with a mean decrease of 40.6%–50.7%in length relative to the wild type under the two growth conditions.Our study demonstrates the quantitative involvement of Bna SVP copies in the regulation of flowering time and provides valuable resources for rapeseed breeding.     

Resistance to Sclerotinia sclerotiorum in wild Brassica species and the importance of Sclerotinia subarctica as a Brassica pathogen

Brassica crops are of global importance, with oilseed rape (Brassica napus) accounting for 13% of edible oil production. All Brassica species are susceptible to sclerotinia stem rot caused by Sclerotinia sclerotiorum, a generalist fungal pathogen causing disease in over 400 plant species. Generally, sources of plant resistance result in partial control of the pathogen although some studies have identified wild Brassica species that are highly resistant. The related pathogen S. subarctica has also been reported on Brassica but its aggressiveness in relation to S. sclerotiorum is unknown. In this study, detached leaf and petiole assays were used to identify new sources of resistance to S. sclerotiorum within a wild Brassica ‘C genome’ diversity set. High‐level resistance was observed in B. incana and B. cretica in petiole assays, whilst wild B. oleracea and B. incana lines were the most resistant in leaf assays. A B. bourgeai line showed both partial petiole and leaf resistance. Although there was no correlation between the two assays, resistance in the detached petiole assay was correlated with stem resistance in mature plants. When tested on commercial cultivars of B. napus, B. oleracea and B. rapa, selected isolates of S. subarctica exhibited aggressiveness comparable to S. sclerotiorum indicating it can be a significant pathogen of Brassica. This is the first study to identify B. cretica as a source of resistance to S. sclerotiorum and to report resistance in other wild Brassica species to a UK isolate, hence providing resources for breeding of resistant cultivars suitable for Europe.     

A Multivariate Analysis of Soybean Genotypes Grown Under Different Cropping Systems

Nature and magnitude of genetic diversity was assessed using Mahalanobis's D² statistics and canonical analysis in 50 genotypes of soybean grown in monoculture and in association with maize. All the genotypes were grouped in 10 clusters in case of monoculture, while 8 clusters were formed for intercropping. Monoculture was more suitable environment for expressing the genetic diversity than intercrop. Some genotypes had consistently the similar clustering pattern in both the cropping systems, while others were affected by the cropping system in expressing the genetic diversity. This was confirmed by the canonical analysis. Days to flowering and maturity, seed yield/plant, plant height and 100-seed weight were mainly responsible for genetic diversity in monoculture. Besides phenological traits, pod length and width, and seed yield/plant exerted marked influence on the genetic diversity of soybean genotypes grown in association with maize. Geographical distribution was not necessarily reflected by the genetic divergence, though some degree of relationship between geographic diversity and genetic diversity was evident under both the cropping systems. The performance of some genotypes varied from cropping system to another, while that of others remained unaffected. Breeding programmes to develop varieties suitable for sole crop, intercrop and both the cropping systems have been suggested.     

Core-sheath polyurethane-carbon nanotube nanofibers prepared by electrospinning

The core-sheath nanofibers consisting of polyurethane (PU) core and PU composites sheath with multi-walled carbon nanotubes (MWNTs) were prepared by electrospinning. At low MWNT concentration, MWNTs appeared highly aligned along the fiber axis with some curving in nanotubes, whereas in case of high concentration, some aggregation of MWNTs appeared due to difficulty in full dispersion of nanotubes. In comparison of the single component nanofiber webs, the core-sheath nanofiber webs showed much better mechanical properties of modulus and breaking stress, including an exceptional elongation-at-break. It indicates that the CNT-incorporated core-sheath structure is very effective for enhancing the mechanical properties of nanofiber webs. In addition, the core-sheath nanofibers demonstrated the fast shape recovery, compared with one component fibers of pure shape memory PU and PU/MWNTs, which provides the possibility of fabricating more sensitive intelligent materials.     

Molecular identification of sex in <Emphasis Type="Italic">Hippophae rhamnoides</Emphasis> L. using isozyme and RAPD markers

In many dioecious plants, gender affects economic value, breeding schemes and opportunities for commercial harvests. Hippophae rhamnoides L. is a dioecious plant species in which female genotypes are commercially preferred over male genotypes. Its berries have rich medicinal, nutritional and pharmaceutical properties because of their large amounts of vitamins, essential oils, proteins, fatty acids, free amino acids and flavanoids. Primary limitation for breeding H. rhamnoides L. is its dioecious nature, since gender cannot be identified by traditional methods. Therefore, some reliable and quick methods need to be developed. This communication deals with the development of isozyme and RAPD markers for early sex identification in this dioecious tree. The isozyme analysis was conducted with four enzyme systems, viz. peroxidase, esterase, malate dehydrogenase and catalase. The peroxidase enzyme system produced a female specific sex marker, which successfully differentiated between the staminate and pistillate genotypes of H. rhamnoides L. Thirty five random decamer primers were used in our study and one male sex linked marker was identified. OPD-20 (5′-ACTTCGCCAC-3′) displayed a band at 911 bp that expressed polymorphism between male and female genotypes. The staminate and pistillate genotypes could be distinguished using RAPD marker OPD-20911. These results revealed the immense potential of peroxidase isozyme patterns and RAPD as genetic markers for sex identification in H. rhamnoides L.     

Standard precipitation index to track drought and assess impact of rainfall on watertables in irrigation areas

The Standard Precipitation Index (SPI) is employed to track drought and assess the impact of rainfall on shallow groundwater levels in three selected irrigation areas of the Murray-Darling Basin in Australia. The continuous SPI method can provide better means of quantifying rainfall variability and correlating it with changes of shallow watertable levels since it is based on continuous statistical functions comparing rainfall variability over the entire rainfall record. Drought analysis in the Australian irrigation areas using SPI indicates that the recent 2000–2006 drought is not the worst drought that has occurred in the recorded history, however if the current low rainfall pattern continues, it would be one of the most prolonged drought. The shallow groundwater fluctuations in the Murrumbidgee Irrigation Area show a very strong correlation with winter rainfall variation. The shallow piezometric levels in the Coleambally Irrigation Area show a weaker degree of correlation with the SPI due to local and regional groundwater dynamics and changes in rice water use. The groundwater levels in the Murray Irrigation Area show least correlation with the SPI, which may be attributed to improved irrigation management practices and complex nature of the groundwater recharge and discharge processes in this area. The overall results however show that the SPI correlates well with fluctuations in shallow ground water table in irrigation areas, and can also capture major drought patterns in Australia. The correlation of SPI with groundwater levels can be adopted for environmental reporting and used as a method of relating climatic impacts on watertables. Differences in piezometric response between years with similar winter and yearly SPI values can be attributed to improvement in irrigators’ management practices.     

Small-Scale Mehedi (<Emphasis Type="Italic">Lawsonia inermis</Emphasis> L.) Farming in the Central Bangladesh: A Promising NTFP-Based Rural Livelihood Outside the Forests

Throughout the world ornamentation of body with mehedi (Lawsonia inermis L.) leaf paste, with the trade name ‘henna body art’ is becoming increasingly popular. The worldwide increasing demand of mehedi leaf encourages many countries to adopt commercial farming of the plant. Farmers living in central Bangladesh recently commenced small-scale mehedi farming, primarily with the purpose of meeting national demand. A total of 182 farmers are engaged in mehedi farming and a study was conducted among 36 of them (a 20% random sample) to explore indigenous management techniques, marketing, livelihood potential and constraints of the enterprise. Farming was reported to be the major primary occupation of the study area. An average of 0.05 ha land of the respondents was used for mehedi cultivation, which constituted 16.7% of their average farm land. The farmers applied their own indigenous technology in every aspect of the farming, using branch cuttings as the only propagation material. The financial analysis indicated that mehedi farming is a profitable and attractive option for rural livelihoods [with an estimated net present value of Tk (Bangladeshi currency unit, 1 US $ = 68 Taka (as of May, 2009).) 179,500 for 0.1 ha plantation]. However, the enterprise faces several constraints, including absence of a nursery for supplying planting materials, storage and effective marketing facilities, available capital for investment, improved technology, and above all, government support. If the government extends cooperation by assisting farmers with training, technology, credit and market development, mehedi farming could become an important revenue-earning enterprise in the small-scale cottage sector of Bangladesh.