Partial characterization of alkaline proteases from viscera of vermiculated sailfin catfish Pterygoplichthys disjunctivus Weber, 1991

Vermiculated sailfin catfish (Pterygoplichthys disjunctivus, Weber, 1991), a member of the Loricariidae family and an invasive species of several inland waters around the world, possess an enormous digestive tract representing about 10% of fish weight. Thus, the aim of this study was to partially characterize proteases from their digestive tracts. Azocasein digestion of the crude extract of intestine at different pH values and temperatures revealed the presence of alkaline proteases with optimum activities at pH 9.0 and 50°C. Incubation assays of the crude extract with inhibitors such as phenyl methyl sulfonyl fluoride, N-α-p-tosyl-l-lysine chloromethyl ketone, N-tosyl-phenyalanine chloromethyl ketone, benzamidine, pepstatin A and ethylenediamine tetra-acetic acid showed that trypsin and chymotrypsin are the main alkaline proteinases present. Zymography showed that the crude extract of Pterygoplichthys disjunctivus viscera contained proteases with molecular masses ranging from 21.5 to 116 kDa. Trypsin and chymotrypsin were inhibited by the following ions in decreasing order: Hg²⁺, Fe²⁺, Cu²⁺, Li⁺, Mg²⁺, K⁺, while Mn²⁺, and Ca²⁺ had no effect. Activities decreased continuously as the NaCl concentration increased from 0 to 30%. These results constitute important background information for future studies and for the potential biotechnological use of the crude digestive extract from this invasive species.     

Effects of tillage systems in dryland farming on near-surface water content during the late winter period

Tillage systems modify, at least temporarily, some of the physical properties of soil, such as soil porosity. Tillage also has an indirect effect on soil water content throughout the growth cycle, particularly in areas with a Mediterranean climate. This paper presents the results of monitoring the water content in the topsoil (0–0.20 m) of three adjacent plots during February to May cycles starting in 1994–1995 and ending in 1998–1999. Each of the plots had a surface area of 2700 m², an 8% slope and Calcic Cambisol soil. Starting in 1994, three different tillage systems were applied: conventional tillage, which is typical of the area (CT); minimum tillage (MT); and no-tillage (NT). Two vertical 200 mm TDR probes were permanently installed in each plot and measurements were taken every week. The results show that, under an NT system, the soil had significantly higher water content than the other two soil plots. However, this increased quantity of water did not denote increased crop production; on the contrary, these preliminary data point to a decrease in crop production.     

Analysis of unbalanced mixed model data: A case study comparison of ANOVA versus REML/GLS

Major transition has occurred in recent years in statistical methods for analysis of linear mixed model data from analysis of variance (ANOVA) to likelihood-based methods. Prior to the early 1990s, most applications used some version of analysis of variance because computer software was either not available or not easy to use for likelihood-based methods. ANOVA is based on ordinary least squares computations, with adoptions for mixed models. Computer programs for such methodology were plagued with technical problems of estimability, weighting, and handling missing data. Likelihood-based methods mainly use a combination of residual maximum likelihood (REML) estimation of covariance parameters and generalized least squares (GLS) estimation of mean parameters. Software for REML/GLS methods became readily available early in the 1990s, but the methodology still is not universally embraced. Although many of the computational inadequacies have been overcome, conceptual problems remain. Also, technical problems with REML/GLS have emerged, such as the need for adjustments for effects due to estimating covariance parameters. This article attempts to identify the major problems with ANOVA, describe the problems which remain with REML/GLS, and discuss new problems with REML/GLS.     

Effects of density profile of MDF on stiffness and strength of nailed joints

Nail-head pull-through, lateral nail resistance, and single shear nailed joint tests were conducted on medium density fiberboard (MDF) with different density profiles, and the relations between the results of these tests and the density profiles of MDF were investigated. The maximum load of nail-head pull-through and the maximum load of nailed joints were little affected by the density profile. However, the ultimate strength of lateral nail resistance, the stiffness, and the yield strength of nailed joints were affected by the density profile of MDF and showed high values when the surface layer of the MDF had high density. It is known that bending performance is also influenced by density profile. Therefore, the stiffness and the yield strength of nailed joints were compared with the bending performance of MDF. The stiffness of nailed joints was positively correlated with the modulus of elasticity (MOE); in the case of CN65 nails, the initial stiffness of joints changed little in response to changes in MOE. The yield strength of nailed joints had a high positive correlation with the modulus of rupture (MOR). The stiffness and the yield strength of nailed joints showed linear relationships with MOE and MOR, respectively.     

Land use alters the abundance of herbivore and predatory insects on crops: the case of alfalfa

We assess the effects of changing land use and crop management on alfalfa insect abundance by comparing it in 50 alfalfa fields when they were inserted in landscapes with different proportions of arable crops and orchards. Land use in a buffer of 500 m was assessed, and alfalfa insect abundance was estimated with sticky yellow traps. The number of catches of several herbivores and predators was related to the proportion of landscape components and several field variables. Results indicated that the proportion of orchards in the buffer negatively affected the abundance of predators on alfalfa, likely because orchards treated with pesticides are a sink for predators moving in the landscape, among other possible causes. Other landscape variables such as noncrop habitats, winter cereals, and landscape diversity analysed by the Shannon index had a minor influence. Among field variables, field size influenced positively the abundance of insects on alfalfa, whereas alfalfa growth stage and age affected positively or negatively the different herbivores and predators. Of course, abundance of predators and prey was affected by the abundance of prey and predators, respectively. These findings suggest that a high proportion of intensively managed crops (orchards) in the landscape interferes with the role of alfalfa as a reservoir of predatory insects for adjacent crops and that the responses to local and landscape structures are temporal and species-specific as previously concluded for maize. Consequently, landscape and field management strategies to improve pest control must consider both types of variables as well as their changing influence when we modify them.

     

Analysis of sulfonamides in animal feeds by liquid chromatography with fluorescence detection

Two analytical methodologies for the simultaneous analysis of eight sulfonamide antibiotics in animal feeds were developed. Analytes were extracted in a simple and rapid procedure by manual shaking with an ethyl acetate/ultrapure water mixture (99:1, v/v) without further sample cleanup. Mean recoveries ranging from 72.7% to 99.4% with relative standard deviations below 9% were achieved from spiked animal feed samples. Determination was carried out by high-performance liquid chromatography using fluorometric detection with precolumn derivatization. The separation of the derivatized compounds was performed using two different chromatographic columns: a conventional C(18) column and a recently available core-shell particle Kinetex C(18) column. Both methods were validated in-house in six different feed matrices, and the two approaches were compared. The experiments showed that the method using the Kinetex column was superior with regard to speed of analysis and precision, both under repeatability and intermediate reproducibility conditions. The limits of detection and quantification were also greatly improved, below 0.10 and 0.34 μg/g, respectively. Finally, this novel approach was successfully applied to the analysis of real feed samples.     

The effect of flushing and stimulus of estrogenized does on reproductive performance of anovulatory-range goats

This study was conducted to determine whether flushing or the stimulus of estrogenized goats is necessary to achieve a high reproductive response in anestrous goats on rangeland. Does were feed-supplemented on rangeland (flushed, n = 20). Other group was exposed to estrogenized does during the mating period (stimulated, n = 20). A third group was both supplemented and stimulated (stimulated–flushed, n = 20) and other group grazed on rangeland only (control, n = 18). More goats in the stimulated and stimulated–flushed groups showed estrus during the first 5 days of joining (45% and 60%, respectively) than the control and flushed groups (11% and 5%, respectively; P < 0.05). Flushing did not improve prolificacy and reduced (P < 0.05) kidding rates (40% and 35% for the flushed and stimulated–flushed groups, respectively) compared to 67% and 55% for the control and stimulated groups, respectively. This experiment demonstrates potential shortcomings of use of flushing in range goats with inadequate nutrition during gestation.     

The Mexico City milk supply system: Structure, function, and sustainability

This paper examines the presentsupply of milk to the urban inhabitant ofMexico City, paying particular attention tocurrent themes of market liberalization,sustainable development, and democratization.This is facilitated by an infrastructure withinand without the metropolitan zone and coexistswith a large importation of milk from theinternational market, much being sold at lowprices to low income groups. Reduced statequality regulation has enabled the use of theseimports in industrialized milk products. Giventhe integration of international and nationalsources in milk supply, simply increasingMexican production will not reduce imports, andthe NAFTA trade pact is unlikely to address theresource exploitation problems faced bynational production.(Researcher from the UK sponsored by the exchange CONACYT-British Council)