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61.
Portnaya I Ben-Shoshan E Cogan U Khalfin R Fass D Ramon O Danino D 《Journal of agricultural and food chemistry》2008,56(6):2192-2198
Beta-casein is an intrinsically unstructured amphiphilic protein that self-assembles into micelles at neutral pH. This paper reports that beta-casein self-organizes into micelles also under acidic conditions. The protein association behavior and micelle characteristics at pH 2.6, well below the p I, are presented. The pH was found to strongly affect the micelle shape and dimensions. Cryogenic transmission electron microscopy (cryo-TEM) experiments revealed disk-like micelles of 20-25 nm in length and approximately 3.5 nm in height in acidic conditions. An aggregation number of 6 was determined by sedimentation equilibrium under these conditions. Isothermal titration calorimetry experiments verified the association below the p I and allowed determination of the micellization enthalpy, the critical micellar concentration, and the micellization relative cooperativity (MR). Small-angle X-ray scattering results at concentrations below the critical micellization concentration (CMC) suggest that the monomeric protein is likely in a premolten globule state at low pH. Calculations of the protein charge at acidic and neutral pH reveal a similar high net charge but considerable differences in the charge distribution along the protein backbone. Overall the results show that beta-casein is amphiphilic at low pH, but the distribution of charge along the protein chain creates packing constraints that affect the micelle organization, leading at concentrations above the CMC to the formation of disk micelles. 相似文献
62.
Cristina Heredia-Acun? Juan J.Almaraz-Suarez Ramón Arteaga-Garibay Ronald Ferrera-Cerrato Deisy Y.Pineda-Mendoza 《林业研究》2019,(5):1727-1734
In this study,10 bacterial strains were isolated from the rhizosphere of coniferous trees on Mount Tla′loc in Mexico.The strains were characterized by their capacity to produce auxins,solubilize phosphates and stimulate mycelial growth of the ectomycorrhizal fungus Suillus sp.All isolates were identified at the molecular level.Moreover,an experiment was established to evaluate the response of Pinus pseudostrobus seedlings to inoculation with the rhizobacteria strains.The isolated strains belonged to the species Cupriavidus basilensis,Rhodococcus qingshengii,R.erythropolis,Pseudomonas spp.,P.gessardii,Stenotrophomonas rhizophila and Cohnella sp.All of the strains produced auxins;the best producer was R.erythropolis CPT9(76.4 lg mL^-1).P.gessardii CPT6 solubilized phosphate at a significant level(443 lg mL^-1).The strain S.rhizophila CPT8 significantly increased the radial growth of the ectomycorrhizal fungus Suillus sp.by 18.8%.Five strains increased the dry mass of the shoots;R.qingshengii CPT4 and R.erythropolis CPT9 increased growth the most,by more than 20%.Inoculation with plant-growth-promoting rhizobacteria can be a very useful practice in a forest nursery to produce healthy,vigorous plants. 相似文献
63.
64.
Enrique Marquez-Rios Ramon Pacheco-Aguilar Victor M. Ocano-Higuera Celia O. García-Sifuentes Susana M. Scheuren-Acevedo 《Journal Of Aquatic Food Product Technology》2013,22(1):105-113
ABSTRACTPostmortem biochemical and microbiological changes in loricariid catfish (Pterygoplichthys disjunctivus) muscle were evaluated during ice storage for 20 days. Values of pH remained stable for 15 days (7.4 ± 0.2), and total volatile base-nitrogen (TVB-N) remained under acceptable limits throughout storage, with a final value of 25.2 ± 3.3 mg N/100 g muscle at Day 20. Adenosine-5´-triphosphate (ATP) and derivatives followed a postmortem degradation pattern similar to other species, with the K-value being the best freshness loss indicator. Biochemical and microbiological changes indicated that the shelf life of iced loricariid catfish muscle was 15 days under optimal (0°C) storage conditions. 相似文献
65.
Georges Choubert José-Carlos G. Milicua Ramon Gomez Sophie Sancé Hélène Petit Geneviève Nègre-Sadargues René Castillo Jean-Paul Trilles 《Aquaculture International》1995,3(3):205-216
Rainbow trout (Oncorhynchus mykiss) with a mean (sd) weight of 120 (2) g were fed diets supplemented with astaxanthin extracted from the yeast Phaffia rhodozyma (OY1 = 50 mg carotenoids kg–1 feed, OY2 = 100 mg carotenoids kg–1 feed), astaxanthin (AX = 100 mg astaxanthin kg–1 feed) and canthaxanthin (CX = 100 mg canthaxanthin kg–1 feed) for 4 weeks. Muscle analyses at the end of the experiment indicated a significantly higher carotenoid concentration in the AX group, while CX and OY1 groups were similar in spite of the differences in dietary concentration. The measure of total muscle colour difference (E*
ab) between initial samples and 4 week ones was higher for the AX fish group but showed no significant difference between OY1, OY2, and CX. The hue and the reflectance ratio (R650:R510) of fish muscle increased in proportion to carotenoid intake. Digestibility (ADC) of yeast astaxanthin in OY1 and OY2 groups was significantly higher than that in the AX group. Canthaxanthin ADC was about one sixth of that of astaxanthin (AX group). Carotenoid retention in the muscle, expressed as a percentage of carotenoid intake, was higher for the AX group than that recorded for OY1 and OY2. According to ADC, carotenoid retention showed a marked lower value for the CX group. Muscle retentions were similar for astaxanthins from both sources. 相似文献
66.
Two elliptical selection experiments were performed in two contemporary sire lines of rabbits (C and R) in order to optimize the experimental design for estimating the genetic parameters of the growth rate (GR) and feed conversion ratio (FCR). Twelve males and 19 females from line C, and 13 males and 23 females from line R, were selected from an ellipse defined by a quadratic index based on these traits. Data from 160 rabbits of each of the parental generations of lines C and R and their offspring (275 and 266 animals, respectively) were used for the analysis. A Bayesian framework was adopted for inference. Marginal posterior distributions of the genetic parameters were obtained by Gibbs sampling. An animal model including batch, parity order, litter size, and common environmental litter effects was assumed. Posterior means (posterior standard deviations) for heritabilities of GR and FCR were estimated to be 0.31 (0.10) and 0.31 (0.10), respectively, in line C and 0.21 (0.08) and 0.25 (0.12) in line R. Posterior means of the proportion of the variance due to common litter environmental effects were 0.14 (0.06) and 0.21 (0.06) for GR and FCR, respectively, in line C and 0.17 (0.06) and 0.22 (0.06) in line R. Posterior means of genetic correlation between both traits were -0.49 (0.25) in line C and -0.47 (0.32) in line R, indicating that selection for GR was expected to result in a similar correlated response in FCR in both lines. 相似文献
67.
A Bayesian analysis with a threshold model was performed for fertility defined as a binary trait (1 = successful mating, 0 = unsuccessful mating) in two populations of rabbits of different reproductive potential and different genetic origin: Line P selected for litter size and Line C selected for growth rate. There were 20,793 records of natural mating (86.2% successful) in Line C between 1983 and 2003, and 17,548 records (80.5% successful) in Line P, between 1992 and 2003. Data related to 5,388 and 3,848 females and 1,021 and 685 males in Lines C and P, respectively. The pedigree included 6,409 and 4,533 individuals in Lines C and P, respectively. The binary response was modeled under a probit approach. The model for the latent variable included male and female additive genetic effects, male and female permanent environmental effects, and the year-season and physiological status of the female (nulliparous, multiparous lactating, or multiparous nonlactating) as systematic effects. Means (standard deviation in parentheses) of the estimated marginal posterior distribution (EMPD) of male heritability were 0.013 (0.006) and 0.010 (0.008) in Lines C and P, respectively, and those of EMPD of female heritability were 0.056 (0.013) and 0.062 (0.018) in Lines C and P, respectively. Means of the EMPD of the proportion of the phenotypic variance due to environmental male and female effects were, respectively, 0.031 (0.007) and 0.128 (0.018) in Line C and 0.053 (0.010) and 0.231 (0.024) in Line P. Means (standard deviations in parentheses) of the EMPD of genetic correlation between male and female fertility were 0.733 (0.197) in Line C and 0.434 (0.381) in Line P. The posterior distribution of genetic correlations presents a huge dispersion, and the estimates should be taken with caution because of the almost negligible estimate of the male genetic component. Results indicate that little genetic variation exists for female fertility, and practically none for male fertility. It would, therefore, be possible to improve reproductive performance by including female fertility in a breeding program, but response to selection would be very small. 相似文献
68.
Nathalie Jiatsa Donfack Kele Amaral Alves Benner Geraldo Alves Rebeca Magalhes Pedrosa Rocha Jamily Bezerra Bruno Carlos H. Lobo Marcelo Bertolini Regiane Rodrigues dos Santos Marilia de Oliveira Taumaturgo Ramon da Silva Raposo Jos Ricardo de Figueiredo Johan Smitz Ana Paula Ribeiro Rodrigues 《Reproduction in domestic animals》2019,54(2):216-224
The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant. However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant. Follicular density significantly decreased in all treatments in comparison with fresh control. We observed high fibrosis in both fresh transplant and vitrified transplant. The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control. In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft. 相似文献
69.
A. Melese Endalew C. Debaer J. Vercammen H. Ramon P. Verboven 《Computers and Electronics in Agriculture》2010,71(2):128-136
The current trend in modelling flow phenomena within trees such as in orchards follows the assumption of the space occupied by the trees as a porous and horizontally homogeneous medium to avoid the flow details associated with the individual plants. This being sufficient at a larger field or regional scale much has to be done at a plant scale to analyse the flow details within the plant and its elements especially for sensitive agricultural operations such as spraying. This article presents an integrated 3D computational fluid dynamics (CFD) model of airflow from a two-fan air-assisted cross-flow orchard sprayer through non-leafed orchard pear trees of 3 m average height. In this model the effect of the solid part of the canopy on airflow was modelled by directly introducing the actual 3D architecture of the canopy into the CFD model. The effect of small canopy parts, such as very short and thin branches and flowers that were not incorporated in the geometrical model, on airflow was simulated by introducing source-sink terms in the Reynolds averaged Navier-Stokes (RANS) momentum and k-? turbulence equations in a sub-domain created around the branches. This model was implemented in a CFD code of ANSYS-CFX-11.0 (ANSYS, Inc., Canonsburg, PA, USA). In this work it was possible to link the real 3D architecture of pear canopy into a CFD code of CFX. The model was able to capture the local effects of the canopy and its parts on wind and sprayer airflow directly by inserting the tree structure into the model which gave realistic results. The model showed that within the injection region of the sprayer there was an average reduction of the jet velocity by 1 m s−1 for a distance of 2.3 m from the sprayer outlet due to the presence of leafless pear canopy. This reduction was variable at different vertical positions due to the difference in the canopy density. Maximal effect of the canopy was observed in the middle height of the trees between 0.25 m and 2.5 m which is the denser region with a bunch of several branches. The maximum velocity difference observed between these two positions was 1.35 m s−1 at 1.75 m height. Thus, regions of high and low air velocity zones of the sprayer due to the variable branch density of the pear tree were predicted. The effects of wind speed and direction on the air jet from the sprayer were investigated using the model. For a cross- (direction of 90°) wind speed of 5 m s−1 there was about 2 m s−1 reduction in the sprayer jet velocity at the jet centre and 0.5 m horizontal shift of the jet centre towards the wind direction. Generally there was a decrease in the jet velocity with increasing cross-wind and decreasing wind direction with respect to the jet direction. 相似文献
70.
Impact of no-tillage agricultural systems on sediment yield in two large catchments in Southern Brazil 总被引:1,自引:0,他引:1
Elizeu Jonas Didoné Jean Paolo Gomes Minella José Miguel Reichert Gustavo Henrique Merten Leandro Dalbianco Cláudia Alessandra Peixoto de Barrros Rafael Ramon 《Journal of Soils and Sediments》2014,14(7):1287-1297