Notes on the spectrophotometric determination of lignin in wood samples

A method developed by Johnson et al. whereby lignin is determined spectrophotometrically was found to yield lignin values which showed variations of up to 10%. The presence of water in, and the freshness of the digestive mixture together with the grade of acetic acid used and the time between dilution and measurement of absorbance were found to affect the reproducibility of the method.     

Analysis of pharmaceutically relevant taxoids in wild yew trees from Sardinia

Needles from a series of wild yews (Taxus baccata L.) from Sardinia were investigated for their contents of 10-deacetyl baccatin III (DAB-III, 1), paclitaxel (Taxol) (2) and taxine (3). Despite a common geographical origin, ample variation of the taxoid profile was discovered, and several samples were surprisingly devoid of all terpenoid markers above. This finding is unprecedented within the European yew, while the general lack of taxine might rationalize the observation that most plants investigated are actively and impunently browsed by goats.     

Effects of nitrogen and phosphorus fertilization on the growth and nutrition of hybrid poplars on Vancouver Island

The effects of nitrogen (N) and phosphorus (P) rates, P source, and method of P application were tested on growth and nutrition of four clones of hybrid poplar (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex Marsh or P. trichocarpa×P. maximowiczii A. Henry) in a plantation on Vancouver Island. Treatments were applied shortly after planting. Nitrogen (0, 500kgNha^–1) was supplied as ammonium sulfate (AS); half of the AS was added at the start of the second growing season. Phosphorus (0, 100, 200kgPha^–1) was supplied as triple super phosphate (TSP), rock phosphate (RP), or diammonium phosphate (DAP) and banded or broadcast. Over four growing seasons, stem volumes increased with AS and P additions. The effects of AS and P each were greatest when the other was also added. Volume was greater when P was applied at 100kgha^–1 than in the P control and did not significantly increase further at 200kgPha^–1. Phosphorus additions were more effective when added as TSP or DAP than when added as RP, but only in P100 and when banded. Banding increased volume when P was applied at 200kgha^–1, but increases were significant only for DAP fertilized trees. Roto-tilling associated with the banding treatment also increased volume when AS and P were not added. Rankings of clones with respect to stem volume varied with N supply and changed over time.     

Regeneration of timber trees in a logged tropical forest in North Bolivia

For sustainable forest management, it is important to know the response of timber species to the change in environment caused by logging. We performed a 2-year study on germination, survival and growth of four timber species, Cedrela odorata, Swietenia macrophylla, Hymenaea courbaril, and Cariniana micrantha, and one non-commercial species Tachigali vasquezii. We sowed seeds of these species in five microenvironments: log landing, gap-crown and gap-trunk, skidder trail and understory, in a tropical lowland moist rain forest in northern Bolivia. We related seed and seedling performance to light availability, soil compaction, and plant competition. Germination did not differ significantly between microenvironments but survival of germinated seeds for most species was significantly higher (P < 0.05) in the log landing (46–100%) than in the understory (0–7%). After 2 years, the tallest plants were always found in the log landing (119–190 cm) and the smallest in the understory (12–26 cm) caused by a higher relative height growth rate (RHGR) in the log landing (0.003–0.004 cm cm⁻¹ per day) compared to the understory (0.000–0.001 cm cm⁻¹ per day). During the first year RHGR was positively related to canopy openness for all species and negatively to the number of overtopping competitors for three species. During the second year also water infiltration explained observed variation to RHGR. These results show that abandoned log landings and logging gaps are suitable environments for the regeneration of timber species studied. This finding suggests that the removal of competitors in log landings and logging gaps combined with leaving seed trees near these microenvironments or sowing seeds, will improve regeneration of timber species in tropical forests.     

Relationships between preference, rumen degradability, gas production and chemical composition of browses

The aim of this work was to assess whether degradability, gas production or chemical constituents could predict the preference of browses. Forty tropical browse species leaves with a crude protein (CP) content ranging from 79 to 307 g kg^-1 DM were used for this study. The neutral detergent fibre (NDF) ranged from 220 to 694 g kg^-1 DM, while acid detergent fibre (ADF) ranged from 146 to 523 g kg^-1 DM. The NDF-bound nitrogen (NDFN) and ADF-bound nitrogen were particularly high in Calliandra calothyrsus, Acacia polyacantha, Sesbania sesban, Acacia venosa and Acacia hockii. High levels of tannins were observed in Acacia species especially A. dolichocephala, A. hockii, A. microbotrya and A. salicina. High levels were also observed in Flemingia macrophyla and Leucaena pallida. The browse species differed (P < 0.05) in DM in sacco degradability coefficients. High potential degradability (PD) and effective degradability (ED) were observed in Sesbania spp, Moringa stenopetala, Indigofera arrecta, Chamaecytisus palmensis and Atriplex spp. The browses differed (P < 0.05) in asymptotic gas (Ag) production (ml g^-1 OM), but had similar (P > 0.05) times of incubation at which half of the asymptotic gas had been formed. Preference and DM intake were positively correlated (P < 0.01) to NDFN, but negatively correlated (P < 0.05) to NDF and ADF. The PD and Ag were negatively (P < 0.001) related to NDF, ADF and lignin. Total phenols (TP) and condensed tannins (CT) were negatively (P < 0.05) related to PD, ED and Ag. A positive correlation was observed between CT and NDF-bound condensed tannins (r = 0.55, P < 0.001) and, CT and TP (r = 0.40, P < 0.01). Prediction equations were poor for DM intake and preference, moderate for gas production and good for potential and effective degradabilities. The phenolic components were more related to dry matter degradation and gas production than to preference and dry matter intake. NDFN and Ag made a positive contribution to both preference and DM intake. It was concluded that chemical constitutes such as N, NDF, NDFN, ADF and lignin are essential to predict the nutritive value of browses. This revised version was published online in June 2006 with corrections to the Cover Date.     

Productivity of intensified crop—fallow rotations in the Trenbath model

Management of crop—fallow rotations should strike a balance between exploitation, during cropping, and restoration of soil fertility during the fallow period. The Trenbath model describes build-up of soil fertility during a fallow period by two parameters (a maximum level and a half-recovery time) and decline during cropping as a simple proportion. The model can be used to predict potential crop production for a large number of management options consisting of length of cropping period and duration of fallow. In solving the equations, the model can be restricted to sustainable systems, where fallow length is sufficient to restore soil fertility to its value at the start of the previous cropping period. The model outcome suggests that the highest yields per unit of land can be obtained by starting a new cropping period after soil fertility has recovered to 50–60% of its maximum value. This prediction is virtually independent of the growth rate of the fallow vegetation. The nature of the fallow vegetation (natural regrowth, planted trees, or cover crops) mainly influences the crop yield by modifying the required duration of fallow periods. Intensification of land use by shortening fallow periods will initially increase returns per unit land at the likely costs of returns per unit labor. When fallows no longer restore soil fertility to 50% of the maximum, overall productivity will decline both per unit land and per unit labor, unless external inputs replace the soil fertility restoring functions of a fallow.This revised version was published online in November 2005 with corrections to the Cover Date.     

Why extensive research and development did not promote use of peach palm fruit in Latin America

Agroforestry Systems - Peach palm (Bactris gasipaes) was domesticated as a fruit crop by the first Amazonians in traditional Neotropical agroforestry systems, but research and development...     

Ecosystem services from coffee agroforestry in Central America: estimation using the CAF2021 model

The goal of sustainable coffee production requires multiple functions from agroforestry systems. Many are difficult to quantify and data are lacking, hampering the choice of shade tree species and agronomic management. Process-based modelling may help quantify ecosystem services and disservices. We introduce and apply coffee agroforestry model CAF2021 (https://doi.org/10.5281/zenodo.5862195). The model allows for complex systems with up to three shade tree species. It simulates coffee yield, timber and fruit production by shade trees, soil loss in erosion, C-sequestration, N-fixation, -emission and -leaching. To calibrate the model, we used multivariate data from 32 different treatments applied in two long-term coffee agroforestry experiments in Costa Rica and Nicaragua. Without any further calibration, the model was then applied to agroforestry systems on 89 farms in Costa Rica and 79 in Guatemala where yields had been reported previously in farmer interviews. Despite wide variation in environmental and agronomic conditions, the model explained 36% of yield variation in Costa Rica but only 15% in Guatemala. Model analysis quantified trade-offs between yield and other ecosystem services as a function of fertilisation and shading.