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Since visual observation is the most commonly used way of detecting oestrus and is supposed to be as effective as detection with technical devices, we evaluated a recently developed oestrus detection scoring system in daily dairy practice. In this scoring system nine signs of oestrus are scored with points, ranging 3 to 100. Twenty-one dairy farmers used the scoring system during a period of 3 weeks. All cows that were more than 30 days post partum and not confirmed pregnant were monitored, using the scoring system, by the herd owners. Oestrus was confirmed by measuring progesterone concentrations in milk. With the scoring system a detection rate of 47% was achieved. This was lower than expected, because in an earlier control period, the detection rate was 64%. We concluded that this new method might be too complicated to introduce to normal herd management, because in daily practice it is too demanding to watch cows twice a day for 30 minutes, especially if the cows show only vague and infrequent symptoms of oestrus. It also appeared to be too complicated to watch the herd at the most appropriate time. However, if the scoring system is included in the daily routine, meaning that farmers are trained to watch for other symptoms than standing heat only and are able to recognize their different values, it can be a valuable aid to oestrus detection. 相似文献
267.
Nucleic acid vaccines: research tool or commercial reality 总被引:3,自引:0,他引:3
Babiuk LA Babiuk SL Loehr BI van Drunnen Littel-van den Hur 《Veterinary immunology and immunopathology》2000,76(1-2):1-23
Polynucleotide immunization has captured the imagination of numerous researchers and commercial companies around the world as a novel approach for inducing immunity in animals. Clearly, the 'proof-of-principle' has been demonstrated both in rodents and various animal species. However, to date, no commercial veterinary vaccine has been developed, or to our knowledge, is in the licensing phase. The present review summarizes the types of pathogens and host species for which polynucleotide immunization has been tried. We have tried to identify possible barriers to commercialization of this technology and areas that need attention if this promising technology is ever to become a reality in the commercial arena. 相似文献
268.
Effects of a porcine reproductive and respiratory syndrome virus infection on the development of the immune response against pseudorabies virus 总被引:5,自引:0,他引:5
De Bruin MG Samsom JN Voermans JJ van Rooij EM De Visser YE Bianchi AT 《Veterinary immunology and immunopathology》2000,76(1-2):125-135
The aim of this study was to investigate the effects of a porcine reproductive and respiratory syndrome virus (PRRSV) infection on the development of the immune response after pseudorabies virus (PRV) vaccination in pigs. Pigs were intranasally inoculated with the European PRRSV strain, Lelystad virus ter Huurne, and were vaccinated intramuscularly with PRV 2 weeks later (LV-PRV group). Control pigs were vaccinated with PRV only (PRV group). Eight weeks after PRV vaccination, pigs from both groups were challenged intranasally with wild-type PRV. We measured the lymphoproliferative, and the cytolytic responses to PRV of peripheral blood mononuclear cells (PBMC), isolated from blood samples. In addition, serum samples were examined for antibodies against PRV and LV. One week after PRV vaccination, PBMC proliferated abundantly to PRV in both groups. However, in the LV-PRV group the lymphoproliferative response declined after 1 week, whereas, in the PRV group, the lymphoproliferative response was high for 3 weeks and declined thereafter (P<0.05). After challenge, the lymphoproliferative response was 1 week earlier and was consistently and significantly higher in the PRV group than in the LV-PRV group. The PRV-specific killing was higher at 3 weeks after PRV vaccination and 5 weeks after PRV challenge 19+/-3 and 24+/-6%, respectively, in the PRV group, compared to 7+/-4 and 6+/-9%, respectively, in the LV-PRV group (P<0.05). However, later after vaccination and challenge the cytolytic response was identical in both groups. The antibody titre against PRV developed equally in both groups. After challenge, no PRV virus was isolated from both groups. From these results we conclude that, although PRRSV infection did cause changes in the time course of the T-lymphocyte response after PRV vaccination, PRRSV infection did not inhibit the development of vaccine-induced protection after PRV. 相似文献
269.
van Rooij EM Haagmans BL Glansbeek HL de Visser YE de Bruin MG Boersma W Bianchi AT 《Veterinary immunology and immunopathology》2000,74(1-2):121-136
Glycoproteins B (gB), gC and gD of pseudorabies virus (PRV) have been implicated as important antigens in protective immunity against PRV infection. As cell-mediated immunity plays a major role in this protective immunity, we determined the significance of these glycoproteins in the actual induction of cell-mediated immunity. We vaccinated pigs with plasmid DNA constructs coding for gB, gC or gD and challenged them with the virulent NIA-3 strain of pseudorabies virus. Vaccination with plasmid DNA coding for gB induced the strongest cell-mediated immune responses including cytotoxic T cell responses, whereas plasmid DNA coding for gD induced the strongest virus neutralising antibody responses. Interestingly, vaccination with gB-DNA reduced virus excretion early after challenge infection while vaccination with gC-DNA or gD-DNA did not.This is the first study to demonstrate that DNA vaccination induces cytotoxic T cell responses in pigs and that cell-mediated immunity induced by vaccination with gB-DNA is important for the reduction of virus excretion early after challenge infection. 相似文献
270.
Niewold TA van Essen GJ Nabuurs MJ Stockhofe-Zurwieden N van der Meulen J 《The Veterinary quarterly》2000,22(4):209-212
Diseases are often thought to result from a single cause. Although this is sometimes the case, e.g. with a highly virulent infection such as Classical Swine Fever (CSF), more often clinical disease in swine herds results from multiple predisposing factors. This is especially true in modern intensive pig husbandry, in which the role of highly infectious diseases is limited to (nonetheless devastating) outbreaks. More important nowadays are diseases, although associated with an agent, without a clear pathogenesis. The emphasis in disease control thus far has been on treatment, eradication and prevention. This has been achieved by focusing attention on husbandry factors, such as climate, housing, hygiene, management, and nutrition. Although this approach has been successful for a number of diseases, several health problems are persistent. There are strong indications that in the latter, intrinsic animal factors are important. Successful handling of these problems requires knowledge of the (patho)physiology of the pig. In this article, several characteristics of pig physiology associated with the occurrence of disease are described. It appears that the modern (fattening) pig is exceptional among other animal species in that its cardiovascular system is mismatched to its body weight. It is argued that this particular disposition causes relatively minor disturbances to have major consequences in the pig. This concept of pig physiology is central to the understanding of the hitherto poorly understood pathogenesis of several diseases, such as oedema disease. 相似文献