Growth performance and physiological parameters of conventional and specified pathogen‐free rats fed autoclaved diets with different protein sources

The effects of feeding autoclaved commercial SSNIFF (SN) diet and diets containing soya bean (S) and casein (C) to growing conventional (CON) and specified pathogen‐free (SPF) rats were determined. Diets S, C and SN, autoclaved at 121 °C during 20 min (T1), at 134 °C during 10 min (T2) and non‐autoclaved (T0), were fed during four weeks, each to 8 CON males and 8 females of mean initial body weight 56 g, kept individually. Diets S, C and SN, autoclaved at T1, were fed during two months, each to 20 SPF males and 20 females of mean initial body weight 58 g, kept in group of 5 animals per cage. In CON rats, autoclaving did not affect feed intake and weight gain, decreased thyroid and stomach weight, increased caecal tissue and digesta weight, and concentrations of isobutyric, isovaleric and valeric acid in caecal digesta. Among biochemical blood parameters, autoclaving decreased only total protein concentration and aspartate aminotransferase activity. Feeding C diet resulted in lower feed intake and weight gain in CON and SPF males. Diet affected organ weights and the greatest differences were found in rats on SN diet for weights of stomach, caecum and female reproductive organs. Diet affected concentration of all short‐chain fatty acids, pH and weight of caecal digesta, the most important being the greatest butyric acid concentration on SN diet and isoacids on C diet. It is concluded that autoclaving of both soya‐containing and soya‐free diets does not affect negatively animal performance and physiology.     

Social behavior in preweaning lambs and their preferences in social interactions

The aim of this study was to investigate social behavior in preweaning lambs, especially their preferences in social interactions. A total of 30 lambs (16 Polish Milk Sheep 05 and 14 Polish Whiteheaded Mutton Sheep) were observed for 113 h 30 min during the first 2 months of their lives. Interbreed differences were insignificant both in agonistic and affiliative behavior. Males appeared to be more active both in agonistic and affiliative interactions. They also preferred other males in agonistic interactions. On the other hand, females fought more often with males, than with females. Lambs of both breeds preferred individuals of their own breed as partners in affliative behaviors. Twins preferred most their siblings in affliliative interactions and usually did not attack and fight with them. This study shows that sex and breed can influence social preferences in preweaning lambs.     

Hypothermic storage of equine isolated hepatocytes

The aim of the study was to establish the optimal methods for hypothermic storage of equine isolated hepatocytes. Viability of equine isolated hepatocytes after hypothermic storage was dependent on the type of storage medium as well as on the cell density in the storage suspension and the preservation period. Hepatocytes stored at 4 degrees C in Hanks' Balanced Salt Solution (HBSS) and Williams' Medium E (WE) for 24 h showed very low viability, numerous cell membrane blebs, very low attachment rate (11.9 +/- 6.5% and 34.8 +/- 19.1%, respectively) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction rate (6.4 +/- 3.9% and 25.1 +/- 14.8%, respectively). In contrast, hepatocytes stored in University of Wisconsin Solution (UW) after 24 h of storage at a density of 12.5 x 10(6) cells/ml showed high viability (over 70%), typical and intact morphology, high cell attachment rates and MTT reduction. Our findings clearly demonstrate that UW is a good preservation solution for equine isolated hepatocytes. Hepatocytes harvested from slaughterhouse organs can be stored at 4 degrees C in UW at a density of 12.5 x 10(6) cells/ml for at least 24 h without significant decrease in functional integrity.     

Impact Assessment of the Upper Silesian Industrial Region on Greenhouse Gas Emissions in Poland

This paper is devoted to the assessment of the impact of the Upper Silesian Industrial Region on greenhouse gases emissions in Poland. Detailed analysis will cover emissions of methane and carbon dioxide which are characteristic for the mining and power engineering industry prevailing in this region. The industries of Silesia emit 16.2% of CO₂, and about 30% of methane emissions in Poland come from coal seams. The leading strategy to counteract this situation, not only in Upper Silesia, should involve the improved efficiency of energy use and decreased use of primary energy carriers.     

Activity Disequilibrium between 234U and 238U Isotopes in Southern Baltic

The aim of the work was to determine the concentration of ²³⁴U and ²³⁸U and calculate the values of the ²³⁴U/²³⁸U activity ratio in samples of living organisms, bottom water, surface and interstitial water and also sediments from the various regions of southern Baltic Sea. The knowledge of ²³⁴U/²³⁸U activity ratio in ecosystem allows getting know about the mechanisms and processes of uranium transport and origin.The activities of the analyzed uranium radionuclides in samples were measured using alpha spectrometry. The results of researches revealed diversified concentrations of uranium in the sediments of the southern Baltic Sea (sea and coastal waters) and increase of uranium with sediment depth, suggesting the diffusion of uranium from sediments to water through interstitial water and diagenesis processes in sediment material. The nuclides of uranium ²³⁴U and ²³⁸U were radioactive state equilibrium in most of the sediments. The values of the ²³⁴U/²³⁸U activity ratio oscillate around one. In bottom, surface, interstitial water and living organisms mean values of the ²³⁴U/²³⁸U activity ratio are between 1.12 and 1.15. Higher uranium concentration was observed in samples of sediments and seawater after flood in 1997 and torrential rainfalls in 2000–2001. River waters are characterized by higher ²³⁴U/²³⁸U activity ratio. The values of the ²³⁴U/²³⁸U activity ratio equal 1.27 in sediments and 1.34–1.38 in seawater indicate the influence of fresh waters.     

Particulate selenium in the baltic sea atmosphere

In order to determine the concentrations of atmospheric selenium and divide it into size classes, aerosols were collected on nitro-cellulose filters, using the PIXE impactors, at three coastal stations in Preila (Lithuania), Hoburg (Gotland, Sweden) and Kap Arkona (Rugia Island, Germany). The determination of selenium and other elements in samples was made using the TXRF (total-reflection X-ray fluorescence) analysis. Air mass trajectories and the results of selenium studies indicate that the highest concentrations of selenium occur with air masses advecting from western and southwestern Europe, while the smallest concentrations were observed for air masses from north and northeastern Europe. It was confirmed that submicron particles of sizes smaller than 2 μm dominate the transport of suspended selenium. It was revealed that selenium in the air above the Baltic Sea is mainly of anthropogenic origin and originates in the same sources as the trace metals such as Cu, Pb and Zn.     

Modification of oxidative phosphorylation in brain mitochondria during development and aging by the oxygen analog of ronnel

This paper describes the effect of the oxygen analog of ronnel (OAR) on the respiration of brain mitochondria from 4- and 16-day-old and 2- and 18-month-old rats. The oxygen analog of ronnel seems to be an uncoupler of oxidative phosphorylation in brain mitochondria derived from rats at all ages investigated. The results reported here suggest that the sensitivity of mitochondria to the uncoupling effect of OAR is an age-dependent phenomenon. Most sensitive were mitochondria isolated from brains of 18-month-old animals, while the most resistant were mitochondria derived from newborn rats (4 days old), as compared with young adult rats (2 months old). The relation between membrane structure and insecticide action is discussed.