Impact of an experimental PRRSV and Streptococcus suis coinfection on the pharmacokinetics of ceftiofur hydrochloride after intramuscular injection in pigs

This study determined the impact of porcine reproductive and respiratory syndrome virus (PRRSV) and Streptococcus suis coinfection on the pharmacokinetic (PK) profile of ceftiofur hydrochloride in pigs after intramuscular (i.m.) injection. Eighteen clinically normal crossbred gilts were assigned by weight into a challenge group (10 pigs) and control group (eight pigs). Pigs in both groups received a single i.m. injection of ceftiofur hydrochloride (Excenel RTU Sterile Suspension; Zoetis) at a 5 mg/kg BW dose. Serial blood samples were collected to characterize the plasma concentration curve. After a 10 days drug washout period, the challenge group was inoculated with 2 mL of PRRSV isolate VR‐2385 (10^5.75 50% tissue culture infective doses per mL) intranasally and 8 days later inoculated S. suis. When clinical disease was evident, the second PK assessment began in both challenge and control groups. Coinfected pigs demonstrated lower values of AUC and C_MAX, but higher values of Cl/F and Vz/F indicating drug kinetics were altered by infection. The data from this study have implications on ceftiofur treatment regimens in diseased pigs.     

Impact of Endocrine‐Disrupting Chemicals on Reproductive Function in Zebrafish (Danio rerio)

The prevalence of endocrine‐disrupting chemicals (EDCs) in the aquatic environment has been associated with the wide detection of alterations in the development and physiology of vertebrates. Zebrafish, as a model species, has been extensively used in toxicological research. In this review, we focus on recent published evidence of the harmful effects of EDCs on reproductive function in zebrafish, including skewed sex ratio, immature gonads, diminished sexual behaviour, decreased sperm count, reduced spawning and fertilization. These impairments mostly result from disruption to sex‐steroid hormones induced by endocrine disruptors. We also discuss other effects of exposure to EDCs. In EDC exposure research, despite incomplete assessments of altered gonad histopathology and sexual behaviour, these present potential effective biomarkers or pathways for evaluating the reproductive function in zebrafish on EDC exposure. To date, the pernicious effects of some EDCs on the reproductive performance in laboratory zebrafish are well understood; however, similar alterations remain for further determination in wild‐type fish and more kinds of EDCs. More studies should be performed under established scientific regulatory criteria to investigate the impact of EDCs on reproduction in zebrafish. Moreover, further research is required to explain the definite mechanism of sexual differentiation, which helps in understanding the shift of sexual phenotype with EDC exposure.     

人工圈养紫貂(Martes zibellina)行为谱构建及行为节律研究

2014年5月~2015年2月,采用全事件取样法和扫描取样法(scan sampling method)对人工圈养的8只紫貂进行行为学观察,定义了休息、运动、采食、排泄、警戒以及其他行为等行为谱。通过对雌雄紫貂在不同季节行为节律研究发现:除了睡眠行为、卧息行为和玩耍行为外,其他行为在时间分配上均表现出雄性高于雌性,卧息行为和其他行为时间分配雌雄之间差异性显著(P<0.05)。人工圈养紫貂行为的日节律表现为19:00到次日5:00处于活动高峰期,日行为具有一定节律性。不同季节紫貂的日节律表现为夏季最高,冬季最低。     

氯前列烯醇结合亚硒酸钠—VE提高乏情母猪繁殖率试验

试验应用氯前列烯醇(PG)结合亚硒酸钠——VE对36头乏情母猪进行诱情。结果表明，用氯前列烯醇结合亚硒酸钠——VE处理乏情母猪能有效地诱导发情并提高其繁殖力。 1 材料与方法 1.1 试验母猪共计36头，其中8月龄以上，体重达100kg，体况良好的纯种长大未发情母猪14头，2～4胎断奶后30天以上乏情长大母猪22头。 1.2 药物来源氯前列烯醇(PG)：宁波激素制品厂生产，每支2ml；亚硒酸钠——VE粉：衢州兽药厂生产，每包500g，每1g内含亚硒酸钠0.4mg，VE5IU。     

扎龙自然保护区鸟类观赏性分析与观鸟管理对策

观鸟是一项陶冶身心、消除疲劳、接近大自然、科学性很强的活动。扎龙国家级自然保护区内，鸟类资源丰富，是进行观鸟旅游和科普、科研的重要基地，本文主要构建了鸟类观赏性评估体系，并对保护区的鸟类进行了观赏性评估；通过问卷调查分析了观鸟者对自然保护区和鸟类的认知度、喜爱度和观鸟的期望等等；并为保护区更好地开展观鸟旅游和科研、科普活动提出管理建议。     

单色光对蛋白高度、哈氏单位和蛋黄颜色的影响

180只海兰褐蛋鸡分别饲养在蓝、绿、红和白等不同光环境中,试验期为19～45周龄,光照强度为15lx,光照时间为16h。试验结果显示,绿光组蛋白高度最高((7.13±0.04)mm),与白光和蓝光组差异显著(P<0.05);绿光和红光组哈氏单位显著大于白光和蓝光组(P<0.05);白光组蛋黄颜色值(4.6±0.15)最大,与其它组之间差异极显著(P<0.01),但绿光组蛋黄颜色值与周龄间相关系数(r)极显著(P<0.01)。结果表明单色光可影响蛋品质,其中绿光组蛋品质最好。     

VC-2-三聚磷酸酯热稳定性的研究

实验分别在80、90、100、110、120℃和0.05、0.1、0.15MPa大气压下,通过紫外吸收法研究了VC-2-三聚磷酸酯的热稳定性。实验结果表明,VC-2-三聚磷酸酯的热稳定性随着温度的升高而降低,随着压强的升高而降低;同时在温度和压强不变的条件下随着时间的延长而降低,但保留率保持在90%以上。     

Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae is the aetiological agent of porcine pleuropneumonia, a highly contagious and often fatal disease. A candidate live vaccine strain, potentially capable of cross-serovar protection, was constructed by deleting the section of the apxIA gene coding for the C-terminal segment of ApxI toxin of the A. pleuropneumoniae serovar 10 reference strain (D13039) and inserting a chloramphenicol resistance gene cassette. The mutant strain (termed D13039A(-)Chl(r)) produced an approximately 48kDa protein corresponding to the N-terminus of the ApxI toxin, and exhibited no haemolytic activity and lower virulence in mice compared with the parental strain. The mutant was evaluated in a vaccination-challenge trial in which pigs were given two intra-nasal doses of the mutant at 14 days intervals and then challenged 14 days after the last vaccination with either A. pleuropneumoniae serovar 1 (4074) or serovar 2 (S1536) or serovar 10 (D13039) reference strains. The haemolysin neutralisation titres of the pre-challenge sera were significantly higher in the vaccinated pigs than in the unvaccinated pigs. The mortalities, clinical signs and lung lesion scores in the vaccinated pigs were significantly lower than those in the unvaccinated pigs for the serovar 1 challenge. A significantly lower lung lesion score was also observed in the vaccinated pigs, compared with unvaccinated pigs, for serovar 2 challenge. Our work suggests that the mutant strain offers potential as a live attenuated pleuropneumonia vaccine that can provide cross-serovar protection.     

非洲鸵鸟的雌雄鉴别研究

对鸵鸟进行早期的性别鉴定，可以确保期望的鸵鸟雌雄性别比例，雏鸟雌雄分群饲养，加快鸵鸟驯养进程，提高育雏成活率都有着重要意义。本研究采用翻肛法，对人工饲养的1～3月龄育雏期非洲鸵鸟进行试验与研究。结果表明，在1月龄时就可根据有无生殖交媾器及其形态、大小的差别进行雌雄鉴别，打破了通常认为鸵鸟在4月龄时才可以进行性别鉴定的传统做法，为非洲鸵鸟的饲养管理提供了有利的依据。