Molecular markers for late blight resistance breeding of potato: an update

Late blight is the most devastating disease of the potato crop that can be effectively managed by growing resistant cultivars. Introgression of resistance (R) genes/quantitative trait loci (QTLs) from the Solanum germplasm into common potato is one of the plausible approaches to breed resistant cultivars. Although the conventional method of breeding will continue to play a primary role in potato improvement, molecular marker technology is becoming one of its integral components. To achieve rapid success, from the past to recent years, several R genes/QTLs that originated from wild/cultivated Solanum species were mapped on the potato genome and a few genes were cloned using molecular approaches. As a result, molecular markers closely linked to resistance genes or QTLs offer a quicker potato breeding option through marker‐assisted selection (MAS). However, limited progress has been achieved so far through MAS in potato breeding. In near future, new resistance genes/QTLs are expected to be discovered from wild Solanum gene pools and linked molecular markers would be available for MAS. This article presents an update on the development of molecular markers linked to late blight resistance genes or QTLs by utilization of Solanum species for MAS in potato.     

Profiling of virulence associated genes of Pasteurella multocida isolated from cattle

Pasteurella multocida is a causative agent of many major diseases of which haemorrhagic septiciemia (HS) in cattle & a buffalo is responsible for significant losses to livestock sector in India and south Asia. The disease outcome is affected by various host- and pathogen-specific determinants. Several bacterial species-specific putative virulence factors including the capsular and virulence associated genes have been proposed to play a key role in this interaction. A total of 23 isolates of P. multocida were obtained from 335 cases of various clinically healthy and diseased cattle. These isolates were examined for capsule synthesis genes (capA, B, D, E and F) and eleven virulence associated genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA) by PCR. A total of 19 P. multocida isolates belonging to capsular type B and 4 of capsular type A were isolated. All isolates of capsular type B harboured the virulence associated genes: tbpA, pfhA, hgbA, sodC and nanH, coding for transferrin binding protein, filamentous hemagglutinin, haemoglobin binding protein, superoxide dismutase and neuraminidases, respectively; while isolates belonging to capsular type A also carried tbpA, pfhA, hgbA and nanH genes. Only 50 % of capsular type A isolates contained sodC gene while 100 % of capsular type B isolates had sodC gene. The gene nanB and toxA were absent in all the 23 isolates. In capsular type A isolates, either sodA or sodC gene was present & these genes did not occur concurrently. The presence of virulence associated gene ptfA revealed a positive association with the disease outcome in cattle and could therefore be an important epidemiological marker gene for characterizing P. multocida isolates.     

Physiological responses of dietary tryptophan fed Labeo rohita to temperature and salinity stress

Two experiments were conducted to elucidate the possible effects of dietary L‐tryptophan (TRP) in Labeo rohita based on growth performance and physio‐biochemical responses. In the experiment I, a 60‐day feeding trial was carried out to elucidate the effects of dietary TRP enrichment on growth performance and physio‐biochemical responses. In the experiment II, the TRP pre‐fed L. rohita, from experiment I, was exposed to temperature and salinity stress to evaluate stress‐mitigating efficacy of TRP. In L. rohita, dietary supplementation of TRP showed significant effect on weight gain percentage and feed conversion ratio but not on blood glucose. A significant increase in RNA content and RNA/DNA ratio upon TRP supplementation was observed and was positively correlated with growth performance. The results of experiment II indicated that weight gain percentage, serum T3 and T4 levels were significantly reduced in groups that were exposed to temperature and salinity stress and fed diets without TRP supplementation. However, dietary supplementation of TRP significantly augmented weight gain percentage in stress‐exposed groups. Tryptophan supplementation helped in bringing back T3 and T4 levels comparable with control. A significant increase in superoxide dismutase, catalase, Adenosine triphosphatase, blood glucose and serum cortisol was observed in temperature‐ and salinity‐exposed groups fed without TRP‐supplemented diets. However, TRP supplementation was found to be effective in restoring the above parameters. The results of these experiments suggest that dietary TRP supplementation augments growth, lowers energy demand and helps in mitigating thermal and salinity stress in L. rohita.     

Genetic studies on primary antibody response to sheep erythrocytes in guinea fowl

1. The primary antibody response to sheep erythrocytes was determined by haemagglutination test in guinea fowl. The effects of various genetic and non‐genetic factors on immune response to sheep RBCs in guinea fowl were also estimated.

2. The immune response to sheep RBCs was normally distributed in guinea fowl with mean titre at 1.534 ± 0.014.

3. In guinea fowl, effects on titre values of sire and variety (feather colour) were significant whereas sex and sex × variety interaction effects were non‐significant.

4. The estimate of heritability for immune response to sheep RBCs in guinea fowl was 0.35 ±0.17.     

The Effect of Intercropping Lablab purpureus L. with Sorghum on Yield and Chemical Composition of Fodder

In two years the growth and composition of mixtures of sorghum with Lablab purpureus as strips, paired rows and alternate rows were compared with those of sorghum and lablab monocrops. In the first year, when sowing did not occur until 7 August, the sorghum yields were low in the monocrop and even less in the mixed crops. Lablab yield was also reduced in mixtures compared with the monocrop, but total forage yield was greater for the mixed crops compared with sorghum alone. Of the mixed cropping systems, a reduction in the yield of lablab plants and in the phosphorus and potassium content of shed lablab leaves in paired rows suggested that there was more competition for nutrients between lablab plants grown in this treatment. In the second year, earlier sowing increased sorghum growth at the expense of lablab yield in the mixed cropping systems, with the result that total forage yield was not increased when sorghum was intercropped with lablab. However, the crude protein content of sorghum stems and leaf yield were increased in mixed crops, particularly in paired and alternate rows rather than strips, demonstrating that close configuration of the legume and cereal are necessary for the cereal to obtain most benefit from nitrogen fixed by the legume. It is concluded that, when conditions are favourable for rapid sorghum and lablab growth, the sorghum will benefit more when it is grown in paired rows with lablab rather than in strips. However, the close spacing normally adopted for paired rows may encourage competition between lablab plants and increase the requirements for phosphorus and potassium fertilizer.     

Histological components of <Emphasis Type="Italic">Trifolium</Emphasis> L. species related to digestive quality of forage

The microscopic analysis of leaflet, petiole, stem and peduncle of 26 populations of 17 Trifolium species was carried out in order to examine their structural characteristics related to digestibility, to compare them between the populations of forage species and their wild relatives and to evaluate, from histological aspect, the potential of wild-growing species to be used as forages. Forage species had low proportion of the tissues composed of thick-walled cells and high proportion of the tissues composed of thin-walled cells in all examined plant parts. The reduction of the proportion of lignified xylem, sclerenchyma and sclerenchymatous interfascicular parenchyma, were suggested as basic selection criteria in breeding Trifolium species for improved digestibility. T. angulatum, T. medium and T. pannonicum proved to be very similar to the forage species in their histological composition, proportion of thick-walled and thin-walled cells and type of variation of the examined parameters. We believe that these species have some potential for use as forages in their own right, but aditional biochemical, physiological and morphological investigations should be conducted.     

Role of zeolites in persistence of high altitude ferruginous Alfisols of the humid tropical Western Ghats, India

The high altitude ferruginous Alfisols associated spatially with shrink–swell soils on the Deccan basalt plateau and dissected table lands in the Western Ghats of India were analysed for their morphological, chemical and mineralogical properties with a view to comprehend their formation and persistence in the tropical humid climate prevailing since the early Tertiary. The study indicates that despite their acidic pH the soils have high bases and their clay fractions are dominated by interstratified smectite-kaolin. The persistence of these non-kaolinitic and/or non-oxidic Alfisols has been possible due to the presence of base-rich zeolites of amygdoloidal basalt. It suggests that for an open system such as soil, the existence of steady state is a more meaningful concept than thermodynamic equilibrium. The knowledge gained on the role of zeolites in soils provides a check on the reasoning of models on the formation of soils in tropical humid climate. The study also indicates that the supply of bases from zeolites can prevent the soils from losing their productivity even in intense leaching environment.     

Haemato-immunology and histo-architectural changes in <Emphasis Type="Italic">Labeo rohita</Emphasis> fingerlings: effect of dietary aflatoxin and mould inhibitor

A feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (AFB1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of Labeo rohita fingerlings. Three hundred and sixty fishes (avg. wt. 1.48–1.54 g) were randomly distributed into eight treatment groups. Eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or without mould inhibitor were prepared. Haematological parameters like total serum protein, albumin, globulin and A:G ratio were significantly (P < 0.05) reduced with increasing levels of aflatoxin in the diet. However, supplementation of mould inhibitor showed enhanced values when compared to non-supplemented counter parts suggesting ameliorating effects of mould inhibitor on aflatoxin. Total leucocyte count was higher in aflatoxin-treated groups. Histological observations were complementary to haematological parameters. Respiratory burst activity was significantly (P < 0.05) decreased in higher aflatoxin-treated groups but not affected significantly (P > 0.05) due to inclusion of inhibitor alone and/or interaction of aflatoxin level and inhibitor in the diet. From this study, it was concluded that up to 20 ppb aflatoxin level in the diet the haemato-immunological parameters are protected.