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51.
Schueler-Furman O Wang C Bradley P Misura K Baker D 《Science (New York, N.Y.)》2005,310(5748):638-642
The prediction of the structures and interactions of biological macromolecules at the atomic level and the design of new structures and interactions are critical tests of our understanding of the interatomic interactions that underlie molecular biology. Equally important, the capability to accurately predict and design macromolecular structures and interactions would streamline the interpretation of genome sequence information and allow the creation of macromolecules with new and useful functions. This review summarizes recent progress in modeling that suggests that we are entering an era in which high-resolution prediction and design will make increasingly important contributions to biology and medicine. 相似文献
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53.
Huan Wang Kun Phil Kim In Ho Kim 《Journal of animal physiology and animal nutrition》2021,105(2):286-293
This study was conducted to assess the effects of different dose levels of zinc oxide (ZnO) combined with probiotics complex supplementation on the growth, performance, nutrient digestibility, faecal lactobacillus and Enterobacteria counts, noxious gas emissions and faecal score of weaned piglets. A total of 180 crossbred weaning pigs ([Yorkshire × Landrace] × Duroc; 6.61 ± 1.29 kg [mean ± SE]; 28 days old) were used in a 42-day trial. All pigs were randomly allotted to 1 of 4 treatment diets based on initial BW and sex (9 replicate pens/treatment; 2 gilts and 3 barrows/pen). Dietary treatment groups were as follows: CON, basal diet +ZnO 3,000 ppm; BZS, basal diet +ZnO 2,100 ppm +0.1% SynerZymeF10; BZS1, basal diet +ZnO 1,200 ppm +0.1% SynerZymeF10; BZS2, basal diet +ZnO 300 ppm +0.1% SynerZymeF10. During the phase 3, decreasing the ZnO concentration led to a linear reduction in ADG (p = 0.044), and the ADG was lower (p < 0.05) in BZS2 compared with CON treatment during the whole experimental period. The effects of dietary ZnO with probiotics complex were not detected (p > 0.05) on nutrient digestibility, Lactobacillus and E. coli counts, faecal gas emissions and faecal scores. In conclusion, the diet supplementation of ZnO (1,200 ppm) with probiotics complex has been shown to have comparable efficacy to ZnO (3,000 ppm) diet on growth performance, nutrient digestibility, faecal microbiota, noxious gas emissions and faecal score of weaning pigs. 相似文献
54.
Michelle T. Fountain Valerie K. Brown Alan C. Gange William O.C. Symondson Phil J. Murray 《Pedobiologia》2007,51(2):147-158
The effects of chlorpyrifos on aquatic systems are well documented. However, the consequences of the pesticide on soil food webs are poorly understood. In this field study, we hypothesised that the addition of a soil insecticide to an area of upland grassland would impact spider and Collembola communities by decreasing numbers of spiders, consequently, causing an increase in detritivore numbers and diversity.Chlorpyrifos was added to plots on an upland grassland in a randomised block design. Populations of Collembola and spiders were sampled by means of pitfall traps (activity density) and identified to species.Twelve species of Collembola were identified from the insecticide-treated and control plots. Species diversity, richness and evenness were all reduced in the chlorpyrifos plots, although the total number of Collembola increased ten-fold despite the abundance of some spider species being reduced. The dominant collembolan in the insecticide-treated plots was Ceratophysella denticulata, accounting for over 95% of the population.Forty-three species of spider were identified. There were a reduced number of spiders in insecticide-treated plots due mainly to a lower number of the linyphiid, Tiso vagans. However, there was no significant difference in spider diversity between the control and insecticide treatments.We discuss possible explanations for the increase in abundance of one collembolan species in response to chlorpyrifos and the consequences of this. The study emphasises the importance of understanding the effects of soil management practices on soil biodiversity, which is under increasing pressure from land development and food production. It also highlights the need for identification of soil invertebrates to an ‘appropriate’ taxonomic level for biodiversity estimates. 相似文献
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Microbial communities in soil are highly species-rich, recognition of which has led to the view that functional redundancy within communities may buffer many impacts of altered community structure on soil functions. In this study we investigated the impact of long-term (>50 years) exclusion of plant-inputs (bare-fallow treatment) on soil microbial community structure and on the ability of the microbial biomass to mineralise tracer additions of 13C-labelled plant-derived C-substrates. Exclusion of plant-inputs resulted in depletion of soil organic matter (SOM) and a reduction in microbial biomass size. The microbial community structure was also strongly affected, as indicated by the distinct phospholipid fatty acid (PLFA) profiles in bare-fallow and grassland soils. Mineralisation of labile plant-derived substrates was not perturbed by the bare-fallow treatment. The incorporation of labile plant-derived C into PLFA biomarkers was found to differ between soils, reflecting the distinct community structures of the soils and indicating that these substrates were utilised by a broad range of microbial groups. In contrast, the mineralisation of recalcitrant plant-derived substrates was reduced in bare-fallow soil and the fate of substrate-derived C within PLFA biomarkers was, initially, similar between the soils. These results indicate that utilisation of these recalcitrant substrates was a function restricted to specific groups, and that exclusion of plant-derived inputs to soil had reduced the capacity of bare-fallow microbial communities to utilise this substrate type. Therefore, the study suggests that long-term selective pressure on microbial communities, resulting in altered community structure, may also result in altered functional attributes. This structure-function relationship was apparent for utilisation of recalcitrant plant-derived substrates, but not for the more widely distributed attribute of labile C-substrate utilisation. 相似文献
57.
Dr. Phil Rupp 《Journal of pest science》1949,22(3):41-43
Ohne Zusammenfassung 相似文献
58.
OBJECTIVE: To evaluate the use of measuring plasma concentrations of atrial natriuretic peptide (ANP), B-type natriuretic peptide (BNP), and cardiac troponin-I (cTnI) to detect dogs with occult dilated cardiomyopathy (DCM). ANIMALS: 118 client-owned dogs. PROCEDURES: Dogs were prospectively examined by use of ECG; echocardiography; and evaluation of concentrations of ANP, BNP, and cTnI. Occult DCM was diagnosed by evaluation of echocardiographic left ventricular dimensions and detection of ventricular arrhythmias on ECG. Sensitivity and specificity of assays for measurement of plasma concentrations of ANP, BNP, and cTnI to detect dogs with occult DCM were determined. RESULTS: Occult DCM was diagnosed in 21 dogs. A concentration of > 6.21 pg/mL for BNP had a sensitivity of 95.2% and specificity of 61.9% for identifying dogs with occult DCM. In contrast, concentrations of ANP and cTnI had relatively low predictive values. CONCLUSIONS AND CLINICAL RELEVANCE: Blood-based screening for occult DCM in dogs can be accomplished by use of a BNP assay. Additional studies should be performed to optimize this method of screening dogs to detect occult DCM. 相似文献
59.
F.N. Fastier D.Sc. D.Phil. F.R.I.C. R.L.E. Menrath B.V.Sc. B.Sc. A. Sharard M.D. J. Ng M.B. Ch.B. 《New Zealand veterinary journal》2013,61(9):201-204
Abstract Extract The periodic examination of various occupational groups for serological indicators of infection, toxic agents or genetic factors associated with disease is being increasingly recommended and used by health authorities throughout the world (WHO, 1970). While an immunological survey can provide information on the prevalence of infection or immune status of populations, it does not necessarily give direct evidence of the prevalence of disease. For this both epidemiological and clinical data are required. 相似文献
60.
Steven S. Seefeldt Rick A. Boydston Phil N. Kaspari Mingchu Zhang Erin Carr Jeff Smeenk David L. Barnes 《American Journal of Potato Research》2013,90(3):239-244
Aminopyralid is used in Alaska to control certain invasive weed species; however it appears to have an extended soil half-life in interior Alaska resulting in carry-over injury in potatoes. Field studies at three experiment stations in Delta Junction, Fairbanks, and Palmer, Alaska were established to determine the dose–response of weeds and above and below ground potato growth to soil-applied aminopyralid (0, 8, 15, 31, 62, and 123 g ae ha?1) . Both prostrate knotweed and narrowleaf hawksbeard were susceptible to aminopyralid. At Delta Junction and Fairbanks, visual injury of potatoes greater than 25 % was observed at 15 g ae ha?1 aminopyralid, whereas at Palmer visual injury was greater than 40 % at 8 g ae ha?1, the lowest rate tested. Potato tuber production was reduced by aminopyralid at rates of 15 g ae ha?1 and above at both Delta Junction and Palmer. Sub-samples of potato tubers from Delta Junction and Palmer were analyzed for aminopyralid content and grown out to determine if aminopyralid in tubers would reduce subsequent growth. The aminopyralid concentration in potato tubers increased with increasing field application rates, with 30 ppb extracted from tubers grown at the highest application rate (123 g ae ha?1). All plants grown from daughter tubers except from control plots in Palmer exhibited injury symptoms. The number of emerged shoots, and shoot height decreased with increasing aminopyralid concentrations in the tuber, with injury rates greater than 70 % at 8 g ae ha?1. 相似文献