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61.
Review of the measuring precision of the new Meibometer® MB550 through repeated measurements in dogs
Objective A meibometer is a device to measure the delivery rate of lipids on the eyelid margin. The aim of this study is to determine the measuring precision of the new Meibometer® MB550 (Courage-Khazaka electronic GmbH, 50829, Cologne, Germany), linked to a computer, by means of repeated measurements in dogs by different examiners. Procedure Two investigators measured the lipid rate on the eyelid margin in 10 healthy dogs for 10 days. One examiner measured the right eye (OD) and the other measured the left eye (OS) for 5 days. After 5 days, the eyes to be measured were switched between the examiners. The new device was able to record all measurement values as charts and curves in comparison to the previous Meibometer, which displayed only one value. Results Mean meibomium lipid level ± SD in the OD and OS of 10 dogs in 10 days was 211 ± 48 MU and 205 ± 41 MU (meibometer units), respectively. There was no significant difference between OD and OS, but a broad distribution of the values could be found. Gender did not influence the measurements. A significant difference was determined between the measurements in the first 5 days to those in the following 5 days. There was, therefore, also a significant difference in the measurements of the 2 examiners. Conclusion Meibometry is a minimally invasive lipid measuring method on the eyelid margin. Repeated measurement results obtained by two examiners, with the new device Meibometer MB550 linked to a computer, showed a wide range of values. The measuring precision of the new Meibometer MB550 is therefore questionable. 相似文献
62.
Zdenek Hublek Martin Tomeek Marcel Kosina Silvie ikutov Petra Strakov Ivo Rudolf 《Zoonoses and public health》2019,66(8):978-981
West Nile virus lineage 2 (WNV‐2) was detected in the brain of 17 goshawks (Accipiter gentilis) that succumbed to neuroinvasive disease in the Czech Republic during 2018: twelve birds were captive and five wild. Furthermore, two wild sparrowhawks (Accipiter nisus) and three other captive birds of prey (golden eagle Aquila chrysaetos, hybrid saker falcon Falco cherrug × F. rusticolus and Harris's hawk Parabuteo unicinctus) also died due to WNV encephalitis. The 2018 outbreak in Czech raptors clearly reflects a new epidemiological situation and indicates an increasing risk of both raptor and human infection with WNV‐2 in the country. 相似文献
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Gisle K Sverdrup Justin J Meager Anders Fern Jon E Skjraasen Petra Rodewald Anne G V Salvanes Torbjrn Jrvi 《Aquaculture Research》2011,42(10):1539-1548
Studies of contest competition between wild and farmed fish have mostly focused on fish with strongly territorial behaviour. Little is known about species with more plastic social behaviour, such as Atlantic cod (Gadus morhua L.), a species that can either aggressively defend territories or shoal. There is also concern that cod that escape from farms will compete with wild populations. We examined dyadic contest competition between wild and farmed juvenile cod using an intruder–resident experimental set‐up in the laboratory. No prior residency advantage was observed, but the differences between farmed and wild cod were clear. Farmed cod were more submissive than wild cod and fled earlier during contests, which suggests that wild fish often out‐compete farmed intruders. Both fish types initiated aggression earlier against fish of the same background. A multivariate analysis of 11 different behavioural traits indicated that a group of 59% of farmed fish were behaviourally very similar to the 55% most submissive wild fish. These results suggest that wild juvenile cod may be quite robust towards competition for food and shelter from juvenile farmed cod, but further research is needed to verify this pattern. 相似文献
68.
In this paper the residual method is used to determine the disaggregated economic value of irrigation water used in agriculture across crops, zones and seasons. This method relies on the belief that the value of a good (its price by its quantity) is equal to the summation of the quantity of each input multiplied by its average value. By applying this method to the Musi sub-basin; a subdivision of the Krishna basin in India, it was found that the value of irrigation water use is not equated across the crops, zones and seasons. The reasons why there is no sinlge value of irrigation water use are suggested. As farmers do not have perfect knowledge, do not all possess the same resource base, plant different crops for a variety of reasons (some for a financial return on land instead of water and others for sustenance), possess different crop rotation practices and are possibly riks adverse, they all value water differently. However, the results need to be interpreted with care as the crop with the lowest return to water is probably not the one to be sacrificed if water is restricted, since farmers plant crops for a variety of reasons (and sometimes not for the highest return to water that they can achieve). 相似文献
69.
Anette Amtmann Ibrahim Ahmed Petra Zahner-Rimmel Adam Mletzko Lisa Katharina Jordan Martin Oberle Helmut Wedekind Jürgen Christian Sven Michael Bergmann Anna Maria Becker 《Journal of fish diseases》2020,43(2):185-195
In a search for alternative, environmentally friendly and effective disinfecting agents, a commercially available protease—Neutrase®—was tested in this work for inactivation of koi herpesvirus (KHV) and of viral haemorrhagic septicaemia virus (VHSV). For comparison, the stability of these viral pathogens in similar configurations at various pH values and concentrations of peracetic acid or quicklime, typically used for disinfection, was tested. Therefore, virus suspensions were incubated with various concentrations of different agents for 24 hr and the titre of the remaining infectious particles was determined by virus titration. Furthermore, the treatment of both viruses, with the agents at concentrations that were previously appointed as effective, was also examined in the presence of solid material (quartz sand). All procedures investigated in this study, including the protease treatment, were able to reduce the titre of KHV and VHSV below the detection limit of the titration. Although further studies are necessary, this is the first report of the application of a protease for the inactivation of the selected fish pathogens, demonstrating the great potential of the latter for disinfection. 相似文献
70.
James W. Wynne Chris Stratford Joel Slinger Francisca Samsing Megan Rigby Russell McCulloch Petra Quezada-Rodriguez Richard S. Taylor 《Journal of fish diseases》2020,43(1):39-48
Hydrogen peroxide (H2O2) is a commonly used treatment for a range of parasitic diseases of marine finfish, including amoebic gill disease (AGD). While this treatment is partially effective at reducing parasite load, H2O2 can have detrimental effects on the host under certain conditions. Treatment temperature and dose concentration are two factors that are known to influence the toxicity of H2O2; however, their impact on the outcome of AGD treatment remains unclear. Here, we investigated the effects of treatment temperature (8, 12 or 16°C) and dose concentration (750, 1,000, 1,250 mg/L) on the efficacy of H2O2 to treat AGD. We demonstrated that a 20-min bath treatment of H2O2 at all doses reduced both parasite load and gross gill score significantly. Parasite load and gross gill score were lowest in the 1,000 mg/L treatment performed at 12°C. At the high dose and temperature combinations, H2O2 caused moderate gill damage and a significant increase in the plasma concentration of electrolytes (sodium, chloride and potassium). Taken together, our study demonstrates that higher H2O2 treatment temperatures can adversely affect the host and do not improve the effectiveness of the treatment. 相似文献