Dietary xylanase and live yeast supplementation influence intestinal bacterial populations and growth performance of piglets fed a sorghum-based diet

This study was to evaluate the effect of xylanase supplementation and the addition of live yeast, Saccharomyces cerevisiae, on growth performance and intestinal microbiota in piglets. One hundred and eighty commercial crossbred 23-d-old piglets (PIC 417) were sorted by initial BW and allocated to 3 treatments: control (CTR) diet, CTR diet supplemented with xylanase at 16,000 birch xylan units/kg (XYL) and XYL diet supplemented with live yeast (2 × 10¹⁰ CFU/g) at 1 kg/t (XYL + LY). Each treatment had 10 replicates, with 6 animals each. A sorghum-based diet and water were available ad libitum for 42 d of the study. Average daily gain (ADG) and average daily feed intake (ADFI) were measured from 0 to 42 d (23- to 65-d-old) and feed conversion ratio (FCR) calculated. At the end of the study, bacterial identification through 16S rRNA (V3 to V4) sequencing of the ileal and caecal digesta from one piglet per replicate was performed. No treatment effects were observed on ADFI. Pigs offered the live yeast in addition to the xylanase had increased ADG compared with those supplemented with xylanase alone (XYL + LY vs. XYL; P = 0.655). FCR was improved with XYL and XYL + LY compared with CTR (P = 0.018). Clostridiaceae counts in the ileum tended to reduce by 10% with XYL and 14% with XYL + LY compared to CTR (P = 0.07). XYL and XYL + LY increased the counts of Lactobacillaceae in the caecum compared with CTR (P < 0.0001). Dietary supplementation of live yeast combined with xylanase improved growth performance and microbial balance of piglets during the nursery phase.     

Breakpoints for disc diffusion susceptibility testing of bacteria associated with fish diseases: A review of current practice

A survey of the methods being employed to determine antimicrobial susceptibility of bacteria associated with aquaculture was performed on behalf of the Permanent Advisory Network for Diseases in Aquaculture. Thirty-two laboratories in 18 countries responded and 25 reported the breakpoints they used for disc diffusion assays applied to Group 1, non-fastidious organisms isolated from finfish. A total of 117 breakpoints were reported for assays in which the disc contents were those specified by the current standard protocols. Data on the source of these breakpoints and the confidence the laboratories had in them are presented. Overall there was a considerable variation in the breakpoints employed by different laboratories and this variation is discussed in terms of the inter-laboratory precision that can be expected from the application of disc diffusion protocols. This paper discusses the possible clinical significance of the variations in the breakpoints and, where there are available data, the extent to which those in use are consistent with breakpoints suggested by other approaches.The data presented in this paper represent a starting point for the movement towards harmonising breakpoints used in association with the standard disc diffusion protocols that have been proposed for susceptibility testing of bacteria associated with fish diseases.     

Impacts of land use and salinization on soil inorganic and organic carbon in the middle-lower Yellow River Delta

Soil inorganic carbon (SIC) is an important reservoir of carbon (C) in arid, semi-arid, and semi-humid regions. However, knowledge is incomplete on the dynamics of SIC and its relationship with soil organic C (SOC) under different land use types in the semi-humid region, particularly in coastal zones impacted by soil salinization. We collected 170 soil samples from 34 profiles across various land use types (maize-wheat, cotton, paddy, and reed) in the middle-lower Yellow River Delta (YRD), China. We measured soil pH, electrical conductivity (EC), water-soluble salts, and SOC and SIC contents. Our results showed significant differences in both SOC and SIC among land use types. The dry cropland (maize-wheat and cotton) soils had significantly higher SOC and SIC densities (4.71 and 15.46 kg C m^-2, respectively) than the paddy soils (3.28 and 14.09 kg C m^-2, respectively) in the 0–100 cm layer. Compared with paddy soils, reed soils contained significantly higher SOC (4.68 kg C m^-2) and similar SIC (15.02 kg C m^-2) densities. There was a significant positive correlation between SOC and SIC densities over a 0–100 cm soil depth in dry cropland soils, but a negative relationship in the paddy soils. On average, SOC and SIC densities under maize-wheat cropping were 15% and 4% lower, respectively, in the salt-affected soils in the middle-lower YRD than the upper YRD. This study indicated that land use types had great influences on both SOC and SIC and their relationship, and salinization had adverse effect on soil C storage in the YRD.     

土壤基质吸力与土壤微生物活性关系的研究

研究了土壤微生物活性与土壤基膜吸力的关系，将土壤发泡点，即土壤导气率由0突变为非0时的基质吸力，与微生物的最高呼吸活性相联系，试图证明土壤微生物的最高活性发生于略高于土壤发泡点的基模吸力。对粗沙土、细沙土和砂壤土三种轻质地土壤的测定表明，土壤微生物的最高呼吸活性发生在略高于土壤发泡吸力的基质吸力。土壤基模吸力较小时微生物活性到达最高值的速度较慢，土壤基模吸力在发泡点附近时，微生物活性到达最高值的速度较快。     

Brandt's cormorant diet (1994–2012) indicates the importance of fall ocean conditions for northern anchovy in central California

Effective ecosystem‐based management requires a comprehensive understanding of the functional links in the system. In many marine systems, forage species constitute a critical link between primary production and upper trophic level marine predators. As top predators, seabirds can be indicators of the forage species they consume and the ocean processes that influence these populations. We analyzed the diet and breeding success for the years 1994, 2003, 2005, and 2007–2012 of the Brandt's cormorant (Phalacrocorax penicillatus), a piscivorous diving seabird, breeding in central California, to evaluate the extent to which cormorant diet composition relates to prey availability, and how diet composition relates to breeding success and ocean conditions. Cormorant diet was primarily composed of young‐of‐the‐year (YOY) northern anchovy (Engraulis mordax), YOY rockfish (Sebastes spp.), and several species of small flatfish (order Pleuronectiformes). YOY rockfish consumption was positively related to their abundance as measured in a late spring pelagic midwater trawl survey. Northern anchovy appeared to be the most important prey as its consumption was positively related to cormorant breeding success. More northern anchovy were consumed in years where warm‐water conditions prevailed in the fall season before cormorant breeding. Thus, warm ocean conditions in the fall appear to be an important contributing factor in producing a strong year‐class of northern anchovy in central California and consequently a strong‐year class of Brandt's cormorant on the Farallon Islands.     

Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13

The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-γ synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays.