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991.
There are both positive and negative impacts on wildlife associated with wildlife tourism. In Sweden, the endangered Arctic fox is subject to a growing tourist interest. In the Helags mountain region there are guided Arctic fox safari tours that provide visitors with information about the Arctic fox. A survey of five separate groups of visitors in the region revealed that knowledge about the status of Arctic foxes and awareness of the behavioral guidelines for Arctic fox encounters improved after participation in a safari tour and with increasing Arctic fox interaction. We propose a schematic model summarizing the diverse ways in which wildlife tourism affects wildlife and their relative importance for conservation. The Arctic fox population in Sweden is small and sensitive to disturbance, but the positive impacts of Arctic fox tourism seem to compensate for the negative and contribute to their conservation under the current level of tourism pressure.  相似文献   
992.

Objective

To evaluate the transdermal local anaesthetic effect of lidocaine or lidocaine combined with prilocaine or tetracaine in horses.

Study design

Experimental, randomized study.

Animals

A total of five healthy adult warmblood horses.

Methods

Horses were clipped bilaterally at the withers, cranial saddle area and caudal saddle area. Baseline measurements for mechanical superficial sensation via von Frey filaments and nociceptive thermal thresholds were performed. A 5% lidocaine patch (12 hour exposure, treatment L), a lidocaine/prilocaine cream (each 2.5%, treatment LP) and a lidocaine/tetracaine cream (each 7%, treatment LT) were applied (both 2 hour exposure). The same product was applied at the same location bilaterally, but on the right side an epidermal micro-perforation (dermaroller, 1200 needles) was performed prior to application. A total of five more measurements were performed at each location, immediately at the end of exposure time followed by hourly measurements. Thermal thresholds normalized to thermal excursion were analysed. One- or two-way anova and the Wilcoxon signed-rank test were used for statistical analysis with p < 0.05 considered significant.

Results

Epidermal micro-perforation had no enhancing effect. Treatments L, LP, and LT resulted in increased thermal excursion (%) immediately (84.7 ± 12.9; 100.0 ± 0.0; 100.0 ± 0.0) and 1 hour (81.7 ± 66; 86.0 ± 17.7; 87.7 ± 14.4) after the removal of the respective product compared to baseline (66.1 ± 9.3; 69.9 ± 8.3; 76.5 ± 7.8). Superficial mechanical sensation was decreased by the lidocaine-and-tetracaine cream at all time points, and by the lidocaine patch and lidocaine-and-prilocaine cream for three measurements.

Conclusions and clinical relevance

Eutectic mixtures of lidocaine with either prilocaine or tetracaine led to a reduction in thermal nociception and mechanical sensation for up to 2 hours.  相似文献   
993.

Introduction

Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds.

Objective

To investigate potential interbreed variation in serum 5-HT in healthy dogs.

Animals

483 healthy dogs of nine breeds aged 1–7 years.

Methods

Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA).

Results

Median 5-HT concentration was 252.5 (interquartile range = 145.5–390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations.

Conclusions

Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1–7 years. These differences should be taken into account when designing clinical studies.  相似文献   
994.
This study investigated the prevalence of MRSA in samples taken in households, with and without backyard pigs in villages in a rural area of Shandong Province, China. Community‐associated MRSA and livestock‐associated MRSA, belonging to ST59 and ST9, respectively, were identified in both humans and pigs. The genotypic and phenotypic comparison of isolates indicates that bidirectional transmission of MRSA has occurred between humans and pigs in the villages.  相似文献   
995.
In this study, the interaction between crop load and irrigation level on yield, fruit size, skin color and stem-end splitting fruit ratio in the apple cultivar ‘Gala, Galaxy’ grafted on rootstock M9 were investigated. Six irrigation programs were applied during the whole growth season: deficit irrigation (rates of 0.25 kc, 0.50 kc, 0.75 kc), full irrigation (rate of 1.00 kc), excess irrigation (rate of 1.25 kc) and non-irrigation (rates of 0.00 kc of “Class A” pan evaporation coefficient). Four crop loads in each irrigation application were performed by hand thinning after the June drop as a- a low crop load (3 fruits cm?2 TCA), b- a medium crop load (5 fruits cm?2 TCA), c- a heavy crop load (7 fruits cm?2 TCA), and d- an un-thinned crop load (>?7 fruits cm?2 TCA). The total tree yield increased with crop load and irrigation levels. Fruit size was significantly increased by the low crop load. Irrigation increased the fruit size compared to non-irrigation treatment. Further 0.75 kc, 1.00 kc and 1.25 kc irrigation treatments significantly increased the fruit length. Irrigation reduced the fruit flesh firmness. While the low crop load increased the skin red color, it decreased the fruit skin brightness. The yellowness of skin decreased with increasing in the irrigation amount. Irrigation reduced the skin brightness and yellowness, but it increased red color. Crop load and irrigation significantly affected the stem-end splitting fruit ratio. While the splitting fruit ratio increased with a decrease in the crop load, it decreased with an increase in irrigation amount, relatively. Consequently, the low and medium crop load treatments would be beneficial to increase the ratio of marketable fruits without any significant losses in yield for ‘Gala’ apple, especially under 0.75 kc deficit irrigation treatment.  相似文献   
996.
This study was conducted in 2007 and 2008 in order to determine the effects of irrigation frequency on the yield and quality parameters of dwarf trees of the apple cultivar ‘Gala, Galaxy’ in the first and second year of cultivation. Irrigation water was applied at 3?, 5?, 7?, and 10-day intervals as much as the amount of water consumed from the field capacity. Statistical analysis revealed that the effects of irrigation frequency on yield were significant. Since the trees used in the experiment showed mainly vegetative growth, the effects of irrigation frequency on the fruit quality characteristics varied. Irrigation water amount was applied as 355.7–446.5 mm and 359.2–538.9 mm to the experimental treatments in 2007 and 2008, respectively. The evapotranspiration measured was in the range 400.7–491.5 mm in 2007 but in the range 440.2–600.5 mm in 2008, while the yields in the same years ranged from 1.54 to 2.84 t ha?1 and from 2.61 to 6.06 t ha?1. Water use efficiency varied between 2.40 and 4.80 t ha?1mm?1 in the first year of the experiment but between 3.45 and 7.08 t ha?1mm?1 in the second year. The I2 treatment, in which the highest yield and water use efficiency were recorded in both years of the experiment under the experimental conditions, was determined as the irrigation scheduling.  相似文献   
997.
In the present study, selected almond genotypes in Keciborlu/Isparta region by Y?ld?r?m (2007) based on late blooming and superior nut chacteristics were used to evaluate seedling growth performances and the reaction to root knot nematodes. Seeds were planted on perlite medium and stored 60 days at +4 °C in order to break seed dormancy. After germinating, seedlings were transferred to plastic pots to evaluate seedling growth characteristics and remaining seedlings were inoculated with Meloidogyne javanica and Meloidogyne incognita in order to asses resistance status of the selected seedlings. The highest germination percentage was obtained from Genotype 54 (96.1?%), the highest plant was observed from Genotype 9 (62.18 cm), the longest primary root was found from Genotype 54 (30.28 cm), the average secondary root lenght was highest in Genotype 9 (31.04 cm) and the widest root collar was observed in Genotype 33 (8.38 mm). Seedling stem diameter changed between 4.76 mm (Genoype 40) to 7.67 mm (Genotype 33). The lowest variation for stem widht was observed in Genotype 55 (0.58?%) while the lowest variation for seedling hight was found in Genotype 33 (0.50?%). Evaluated almond genotypes showed different reactions to studied nematode species, M. javanica and M. incognita. Resistant reactions of almond genotypes to nematode species were classified as susceptible, tolerant and resistant based on gal index values. Genotype 9 and 31 classified as tolerant to M. javanica while Genotype 54 classified as resistant to M. incognita.  相似文献   
998.
Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 RAPD and ISSR primer investigated, 22 could amplify clearly and consistently. Cluster analysis of the pears genotypes was performed based on data from polymorphic bands RAPD and ISSR by using Jaccard’s similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. The 31 pear genotypes were classified into two major groups. Cluster A was divided into 2 subclusters: Gumushane pears and Trabzon pears. Cluster B consisted of Artvin pears. The similarity matrix values ranged between 0.105 and 0.968.  相似文献   
999.

Context

The variation in spatial distribution between ecosystem services can be high. Hence, there is a need to spatially identify important sites for conservation planning. The term ‘ecosystem service hotspot’ has often been used for this purpose, but definitions of this term are ambiguous.

Objectives

We review and classify methods to spatially delineate hotspots. We test how spatial configuration of hotspots for a set of ecosystem services differs depending on the applied method. We compare the outcomes to a heuristic site prioritisation approach (Marxan).

Methods

The four tested hotspot methods are top richest cells, spatial clustering, intensity, and richness. In a conservation scenario we set a target of conserving 10 % of the quantity of five regulating and cultural services for the forest area of Telemark county, Norway.

Results

Spatial configuration of selected areas as retrieved by the four hotspots and Marxan differed considerably. Pairwise comparisons were at the lower end of the scale of the Kappa statistic (0.11–0.27). The outcomes also differed considerably in mean target achievement, cost-effectiveness in terms of land-area needed per unit target achievement and compactness in terms of edge-to-area ratio.

Conclusions

An ecosystem service hotspot can refer to either areas containing high values of one service or areas with multiple services. Differences in spatial configuration among hotspot methods can lead to uncertainties for decision-making. This also has consequences for analysing the spatial co-occurrence of hotspots of multiple services and of services and biodiversity.
  相似文献   
1000.
A long-term study was carried out in 11 dairy herds in the Khon Kaen province of northeast Thailand between August 2001 and November 2004. The objective was to investigate seroprevalence dynamics of Neospora caninum infection in the herds and to demonstrate patterns of seroconversion in individual cattle. Each herd was visited once a year, in total four times, and sera from cattle > 3 months of age and farm dogs as well as a sample from the bulk milk were collected. All samples were analysed for presence of specific antibodies by an N. caninum iscom ELISA. The overall percentage of antibody-positive cattle was constant and varied only between 10 and 13% over the 4 years, but the variation in within-herd seroprevalence between herds was substantial. Two herds had > or = 20% seropositive animals at all samplings and consistently high bulk milk OD, whereas two herds had no seropositive animal at the last two samplings and low bulk milk OD. Five herds had a decreasing trend of within-herd seroprevalence, whereas the remaining six herds had a higher portion of test-positive individuals at the end of the study. A total of 424 individuals were sampled more than once; 344 (81%) and 32 (8%) were consistently antibody-negative and antibody-positive, respectively. The proportions of animals that changed from being seronegative to seropositive and from being seropositive to seronegative between the years were 3.9-4.6% and 19-39%, respectively. Apparent vertical and horizontal transmission rates were 58% (95% CI; 44-71%) and 5% (95% CI; 3-7%), respectively. In conclusion, the overall percentage of N. caninum antibody-positive cattle was constant over the years, but the within-herd seroprevalence varied substantially between the herds. Seroconversions were likely to occur in individual cattle although most animals had consistent serological status throughout the study.  相似文献   
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