European small pelagic fish distribution under global change scenarios

The spectre of increasing impacts on exploited fish stocks in consequence of warmer climate conditions has become a major concern over the last decades. It is now imperative to improve the way we project the effects of future climate warming on fisheries. While estimating future climate‐induced changes in fish distribution is an important contribution to sustainable resource management, the impacts on European small pelagic fish—representing over 50% of the landings in the Mediterranean and Black Sea between 2000 and 2013—are yet largely understudied. Here, we investigated potential changes in the spatial distribution of seven of the most harvested small pelagic fish species in Europe under several climate change scenarios over the 21st century. For each species, we considered eight Species Distribution Models (SDMs), five General Circulation Models (GCMs) and three emission scenarios (the IPCC Representative Concentration Pathways; RCPs). Under all scenarios, our results revealed that the environmental suitability for most of the seven species may strongly decrease in the Mediterranean and western North Sea while increasing in the Black and Baltic Seas. This potential northward range expansion of species is supported by a strong convergence among projections and a low variability between RCPs. Under the most pessimistic scenario (RCP8.5), climate‐related local extinctions were expected in the south‐eastern Mediterranean basin. Our results highlight that a multi‐SDM, multi‐GCM, multi‐RCP approach is needed to produce more robust ecological scenarios of changes in exploited fish stocks in order to better anticipate the economic and social consequences of global climate change.     

Comparison of sandy soils suppressive or conducive to ectoparasitic nematode damage on sugarcane

ABSTRACT Two South African sandy soils, one suppressive and the other conducive to ectoparasitic nematode damage on monoculture sugarcane, were compared. Analysis of field transects indicated that the suppressive soil displayed a comparatively higher population of the weak ectoparasite Helicotylenchus dihystera, whose predominance among ectoparasitic nematodes is known to limit yield loss caused by more virulent phytonematodes. Soil type was identical at both sites (entisols), but the suppressive soil had a higher organic matter content and a lower pH, which correlated with H. dihystera population data. In contrast, microclimatic differences between the two field sites were unlikely to be responsible for the suppressive or conducive status of the soils, as shown in a greenhouse experiment. The two soils exhibited a bacterial community of the same size but with different genetic structures, as indicated by automated ribosomal intergenic spacer analysis (RISA). The number of culturable fluorescent pseudomonads was higher for the conducive soil, probably because extensive root damage caused by ectoparasitic nematodes favored proliferation of these bacteria. This study shows that apparently small differences in soil composition between fields located in the same climatic area and managed similarly can translate into contrasted nematode communities, ectoparasitic nematode damage levels, and sugarcane yields.     

Reliable ELISAs showing differences between resistant and susceptible lines in hens orally inoculated with Salmonella Enteritidis

Reliable ELISAs were investigated with the aim to select hen lines resistant to Salmonella Enteritidis and producing high levels of antibodies. In the first experiment, the relation between the humoral response and the bacteriological results was assessed on hens from the Y11 resistant line and the L2 susceptible line, orally inoculated with 10(8) CFU S. Enteritidis per animal. Anti-lipopolysaccharide (LPS) IgG titres were higher but the liver and spleen were less contaminated in hens from the Y11 line than in hens from the L2 line (p = 0.013, 0.031 and 0.026 respectively). In the second experiment, the hens were inoculated orally with 1.7 x 10(8) CFU S. Enteritidis per animal in order to select the ELISA methods showing the more significant differences. ELISAs were based on LPS, flagella, LPS from rough (LPS-R) and smooth strains (LPS-S) and detected IgG and IgM antibodies from sera and yolks. No between-line host response variation was observed in the yolk, with LPS-S and R antigens nor with anti-LPS IgM in the sera. Otherwise, significant differences were encountered between hen lines with the ELISAs performed on the sera detecting anti-LPS IgG, anti-flagella IgG or IgM (p = 0.017, 0.017 and p < 0.001 respectively). When comparing the kinetics of the selected ELISAs, the IgG antibodies against LPS detected between-line variations as early as 1 to 4 weeks pi, whereas with IgG against flagella, the differences were only detected at 1 and 2 weeks pi and with IgM against flagella, the differences were significant at 1, 2, 4 and 8 weeks pi. In conclusion, resistant hen lines producing higher levels of antibodies than the susceptible hen lines may be selected with these ELISAs.     

Sustainability and multifunctionality in French farms: Analysis of the implementation of Territorial Farming Contracts

Sustainable agriculture and ways to achieve it are important issues for agricultural policy. However, the concept of sustainability has yet to be made operational in many agricultural situations, and only a few studies so far have addressed the implementation process of sustainable agriculture. This paper provides an assessment of the Territorial Farming Contracts (TFC) – the French model for implementing sustainable agriculture – and aims to give some insights into the ways to facilitate the development of sustainable farming. Using a systems approach, the founding concept of the TFC model, an analysis has been made of the TFCs signed in the Midi-Pyrenees Region in south-western France. The results show that the first aspect of sustainability apparent in farmers’ projects referred to economic objectives. The environmental and social aspects were not foremost in the farming changes undertaken. In addition, the territorial dimension of the TFC was under-addressed. The majority of TFCs reveal a moderate or even low convergence with territorial priorities. These results are explained partly by the dominance of professional farming organizations in the implementation of TFC, and they imply that the organizational social dimensions of sustainability must not be neglected. Mohamed Gafsi is an assistant professor of farm management at the National School of Agronomic Training. He received his PhD in management science at the University of Bourgogne. His research interests include farm management and protection of natural resources, corporate environmental management, sustainable agriculture, and African family farms. Geneviève Nguyen is assistant professor in rural economics at the National Polytechnique Institute – National Superior School of Agronomy, in Toulouse. Her research interests include the dynamics of agrarian institutions in uncertain economies, the supply and organization of services in remote rural areas. Her research has been carried out in Europe, Africa, and Asia. She received her MA in Economics and her PhD in Agricultural Economics from the Ohio State University. Bruno Legagneux is assistant professor of farm management at the National Polytechnique Institute – National Superior School of Agronomy, in Toulouse. His research interests include farm management and the entry of young farmers into farming. Patrice Robin is an engineer agronomist. He received his diploma from the National Superior School of Agronomy, in Montpellier. He is currently a doctoral candidate at the University of Toulouse. His research interests include agriculture and rural development, environmental issues, and food quality.     

Widespread translational inhibition by plant miRNAs and siRNAs

High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.     

Cocoa-based agroforestry system dynamics and trends in the Akongo subregion of central Cameroon

Cocoa is a major crop and income source for most farmers and rural households in the Centre, South and South-West regions of Cameroon, where cocoa is generally produced in agroforestry systems. In this country, cocoa-based agroforestry systems (CBAFS) are undergoing multiple changes alongside the rapid changes underway in the natural, economic and socio-political conditions. This study—carried out in the Akongo subregion in central Cameroon—was designed to gain insight into the CBAFS trends and dynamics in the light of those multiple changes. This semi-structured socioeconomic survey involved interviews and direct observations at plot, farm, household and village scales. Overall, forty cocoa growers from ten villages were interviewed and then fifteen cocoa plots were characterized on the basis of the survey findings. They revealed that cocoa was the major crop in this study area, with cocoa plantations occupying three quarters of the total farming area. Three types of CBAFS were identified, which differed according to their vegetation structure, management practices and age of the plantations. Dynamics affecting the structural characteristics and the spatial extension of these systems emerged and were intimately linked to the dynamics of the cocoa farmer population in relation to their context.

     

High density flow-through culturing of brine shrimp Artemia on inert feeds — Preliminary results with a modified culture system

A modified filter system is described for the intensive culturing of Artemia in a continuously renewed medium. Extrapolated to a 1 m³ tank, 25 kg live weight Artemia could be produced over a culture period of two weeks on a diet of micronized and defatted rice bran using the salt enriched effluent of an abandoned geothermal well as a culture medium.     

Cryptosporidium infection in a veal calf cohort in France: molecular characterization of species in a longitudinal study

ABSTRACT: Feces from 142 animals were collected on 15 farms in the region of Brittany, France. Each sample was directly collected from the rectum of the animal and identified with the ear tag number. Animals were sampled three times, at 5, 15 and 22 weeks of age. After DNA extraction from stool samples, nested PCR was performed to amplify partial 18S-rDNA and 60 kDa glycoprotein genes of Cryptosporidium. The parasite was detected on all farms. One hundred out of 142 calves (70.4%) were found to be parasitized by Cryptosporidium. Amplified fragments were sequenced for Cryptosporidium species identification and revealed the presence of C. parvum (43.8%), C. ryanae (28.5%), and C. bovis (27%). One animal was infected with Cryptosporidium ubiquitum. The prevalence of these species was related to the age of the animal. C. parvum caused 86.7% of Cryptosporidium infections in 5-week-old calves but only 1.7% in 15-week-old animals. The analysis of the results showed that animals could be infected successively by C. parvum, C. ryanae, and C. bovis for the study period. C. parvum gp60 genotyping identifies 6 IIa subtypes of which 74.5% were represented by IIaA15G2R1. This work confirms previous studies in other countries showing that zoonotic C. parvum is the dominant species seen in young calves.