重庆市50年来小麦主栽品种高分子量谷蛋白亚基分析

采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术（SDS-PAGE）．对重庆市近50年来主要推广的50个小麦品种的高分子量谷蛋白亚基遗传变异进行分析,检测到13种亚基和17种亚基组合类型,其中,Glu-A1有3种类型（1、null和2^＊）。分别占50％、46％和4％;Glu-B1有7种类型,主要有7＋8、14＋15和7＋9。分别占54％、18％和12％;Glu-D1有3种类型,主要是2＋12、5＋10。分别占76％、20％;品质评分在5-10之间,平均7．3分;20世纪90年代以后育成品种中优质亚基5＋10、17＋18出现频率明显增加。说明育种单位已重视利用具有优质遗传背景的材料。     

CHRIS数据几何校正精度改善研究(英文)

This paper deals with a new type of multi-angle remotely sensed data--CHRIS （the Compact High Resolution Imaging Spec- trometer）, by using rational function models （RFM） and rigorous sensor models （RSM）. For ortho-rectifying an image set, a rigorous sen- sor model-Toutin＇s model was employed and a set of reported parameters including across track angle, along track angle, IFOV, altitude, period, eccentricity and orbit inclination were input, then, the orbit calculation was started and the track information was given to the raw data. The images were ortho-rectified with geocoded ASTER images and digital elevation （DEM） data. Results showed that with 16 ground control points （GCPs）, the correction accuracy decreased with view zenith angle, and the RMSE value increased to be over one pixel at 36 degree off-nadir. When the GCPs were approximately chosen as in Toutin＇s model, a RFM with three coefficients produced the same accuracy trend versus view zenith angle while the RMSEs for all angles were improved and within about one pixel.     

雏鸡在感染IBDV后血浆cAMP、cGMP和IL-2水平变化

在经ＩＢＤ弱毒苗免疫和未免疫的３０～５９日龄ＡＡ鸡研究了感染ＩＢＤＶ后血浆ｃＡＭＰ、ｃＧＭＰ和ＩＬ２水平动态变化。结果表明：未免疫攻毒鸡（Ａ组）、免疫攻毒鸡（Ｂ组）和未免疫未攻毒鸡（Ｃ组）在ＩＢＤＶ攻毒前１天血浆ｃＡＭＰ和ｃＧＭＰ水平Ｂ组明显高于Ａ和Ｃ组,ｃＡＭＰ／ｃＧＭＰ比值同样是Ｂ组高于或明显高于Ａ和Ｃ组,ＩＬ２水平三组之间无明显差异。在攻毒后的头５天内,Ｂ和Ｃ组ｃＡＭＰ明显升高,而Ａ组没有类似的变化,第５天明显低于Ｂ和Ｃ组,以后Ｂ和Ｃ组仍高于或显著高于攻毒前和同期Ａ组水平。在攻毒后Ａ和Ｂ组ｃＧＭＰ均呈波动性上升趋势,第７天明显高于攻毒前水平并高于Ｃ组,以后Ａ、Ｂ、Ｃ三组均下降,但Ａ组比Ｂ和Ｃ组下降更快。在攻毒后２８天内,Ａ组ｃＡＭＰ／ｃＧＭＰ比值无明显变化,Ｂ组波动上升,Ｃ组处于高水平上,Ｂ和Ｃ组均高于或明显高于Ａ组。血浆ＩＬ２水平Ａ和Ｂ组在ＩＢＤＶ攻毒后的头７天内呈逐渐下降趋势,但Ａ组下降的更剧烈,Ｃ组无明显差异性变化,三组相比,Ｂ和Ｃ组均高于或明显高于Ａ组,以后Ａ和Ｂ组逐渐回升。     

红纹凤蝶半合成人工饲料研究

应用正交试验设计,考察了半合成人工饲料中马兜铃叶粉、麦胚粉加酵母粉、藻粉和防腐剂４因素３水平的不同组合对红纹风蝶幼虫历期、蛹重和化蛹率的影响,筛选出一个可用于大量繁殖的半合成人工饲料配方,在该配方中,幼虫期、蛹重与对照差异不显著,化蛹率极显著高于其它半合成人工饲料配方,各因素不同水平的极差分析表明：防腐剂的含量是影响红纹凤蝶幼虫生长发育的主要因素,此外,寄分呈的变化主要影响幼虫发育历期和蛹重,麦胚     

改善生态环境防治长江上游水土流失

长江上游长约４５００ｋｍ,流域面积约１００万ｋｍ＾２。本区域地理条件特征。水力、矿产、动植物和旅游资源部十分丰富,具有巨大的经济发展潜力。近几十来,由于本区域森林植被遭到严重破坏,垦殖率不断增高以及对资源的不合理开发利用,致使长江上游地区生态环境日益恶化,水土流失不断加剧。在长江上游地区自然生态系统遭到严重破坏的同时,也给长江中下游地区带来了巨大的危害,改善长江上游生态环境和防治水土流失,是实现流     

Molecular analysis of Lathyrus sativus L. (grasspea) and related Lathyrus species

Eight Lathyrus sativus L. accessions from a variety of geographic origins were used to study intraspecific genetic diversity using RAPD analysis. Fourteen decamer primers produced 64 amplification products, 50% of which were polymorphic between the samples. Jaccard's coefficient of genetic similarity was calculated between samples and a dendrogram was constructed by an unweighted pair-group method with arithmetical averages (UPGMA). The dendrogram showed that most of the L. sativus plants clustered into accessions or common geographical areas. The average genetic similarity coefficient within accessions was 0.12 and between accessions was 0.20, indicating a low level of intraspecific genetic variation. Interspecific genetic diversity and phylogenetic relationships of eight Lathyrus species, including L. sativus and Pisum sativum L. (field pea) were examined using 14 decamer primers which produced 283 amplification products. All amplification products were polymorphic across the nine species. In the dendrogram the Lathyrus species clustered into three distinct groups which correlated with the Sections Lathyrus, Clymenum and Linearicarpus. This supports traditional taxonomic classifications of the genus Lathyrus which are based on morphological traits. Of the species from Section Lathyrus, L. gorgoni and L. cicera were the most similar to L. sativus. The results suggest that a strategy of breeding for producing lines of L. sativus with increased genetic variation would be effectively achieved through hybrid production between accessions from wide geographic areas particularly the Mediterranean area and the Indian subcontinent. However, the most effective method would be introgression of germplasm from other species in Section Lathyrus. This revised version was published online in July 2006 with corrections to the Cover Date.     

犬精液冷冻与人工授精的进展

详述了国内外大精液冷冻与人工授精（AI）技术的研究及利用现状,针对大精液的采集、稀释、平衡、冷冻、解冻和输精等主要技术环节进行了总结分析,试图概括本领域已有的成果和经验,找出存在的问题,以利该技术在我国的进一步研究和推广。     

基于WinCE的嵌入式农业环境监测系统

提出了农业环境远程监测系统方案,详细阐述了数据采集模块与串行通信模块的设计方法,建立了由多路传感器采集的数据通过ARM9嵌入式开发平台进行显示,并由串口完成嵌入式平台与PC机的数据通信环境监测系统.实验表明,数据采集及通信工作性能良好,具有很强的通用性.     

中草药免疫增强剂在甲壳动物病害防控中的应用

中草药免疫增强剂具有来源广泛、价格低廉、环境污染较低等优越性,近年来已较多地应用于甲壳类养殖动物的病害防控,产生了较好的效果.本文介绍了中草药免疫增强剂多糖、甙类、生物碱类、有机酸及挥发性油类等免疫活性成分和作用机理,以及在甲壳动物上的应用.总结和分析了中草药免疫增强剂在甲壳动物病害防控中的应用情况、评价指标及目前在研制和应用中存在的主要问题和发展趋势,以期为甲壳动物免疫增强剂的研究和应用提供参考.     

Cultivation of the intertidal brown alga Hizikia fusiformis (Harvey) Okamura: mass production of zygote‐derived seedlings under commercial cultivation conditions,a case study experience

Mariculture of the brown alga Hizikia fusiformis (Harvey) Okamura as an export‐oriented human food has been there more for than 20 years in China. It is now one of the five major farmed algal species along the Chinese coast. Stable and sufficient supply of young seedlings for scaling up the cultivation has been a problem throughout the farming history of this species due to the unique dioecious life cycle and relatively short time window of sexual reproduction in nature. These two factors led to a practical difficulty in obtaining zygotes at identical developmental stage in viable amounts for seedling production. A key solution to this problem is to control the synchronization of the receptacle development and to realize the simultaneous discharge of male and female gametes, such that the fertilization rate could be greatly enhanced. Focusing on one of the farmed populations in this report, we present our results on mass production of seedlings using the synchronization technique on a large scale performed in 2007. Totally 5.5 hundred million embryos were obtained from 100 kg female sporophytes. The seedlings were raised up to 3.5 mm in length in greenhouse tanks over a month and were further grown in open sea for over 3 months at two experimental sites. The success of mass production of seedlings in this alga helped to lay the basis for future trials in other species in the genus of Sargassum that have identical life cycle.