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71.
72.
Shinozuka Y Yamato O Hossain MA Higaki T Ishikawa I Ichiba S Takagi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(2):255-258
Three Japanese Black cows housed with 6 other cows exhibited main clinical symptoms of severe hemoglobinuria. Hematological analyses conducted after antibiotic therapy demonstrated severe anemia, and biochemical analyses indicated both severe hemolysis and disruption of hepatic function. One of the three cows died. Based on the above analyses and observation of typical clinical symptoms, a speculative diagnosis of bacillary hemoglobinuria was made, and immediate high-dose antibiotic treatment improved the general conditions of the surviving animals. Blood samples from the other 2 cows were collected sequentially after antibacterial therapy. Clostridium haemolyticum was detected by a nested polymerase chain reaction analysis of the blood samples. The cows were diagnosed with the second recorded occurrence of bacillary hemoglobinuria in Japan. 相似文献
73.
Adachi I Kusuda S Kawai H Ohazama M Taniguchi A Kondo N Yoshihara M Okuda R Ishikawa T Kanda I Doi O 《The Journal of reproduction and development》2011,57(2):262-266
The purposes of the present study were to establish a noninvasive monitoring assay of fecal progestagen measurement to detect pregnancy and to identify the components of fecal progestagens in early, middle and late pregnancy in cheetahs. Feces were collected from 7 female cheetahs and analyzed from 30 days before the last copulation to parturition in 9 pregnancies. Blood was collected from one cheetah. Fecal progestagen and serum progesterone concentrations were determined by enzyme immunoassay (EIA). The profiles of the fecal progestagen concentrations were similar to the serum progesterone profile. Fecal progestagen and serum progesterone concentrations remained at the baseline until copulation. In the mean fecal progestagen profile during pregnancy (92.8 ± 0.4 days; from the last copulation to parturition), the concentrations increased 3-4 days after the last copulation and remained high until parturition. To investigate changes in the components of progestagen metabolites in the tripartite periods of gestation, fecal progestagens were analyzed by HPLC-EIA. Marked immunoreactive peaks consistent with 5α-pregnan-3α/β-ol-20-one and 5α-pregnan-3,20-dione and small peaks consistent with 5β-pregnan-3α/β-ol-20-one were detected. There were no distinct difference in the components of progestagens among the first, second and third trimesters of pregnancy. The hormone assay, as an indicator of fecal 5α-reduced pregnanes, is useful for detecting pregnancy and monitoring pregnant luteal activity in cheetahs. 相似文献
74.
Toshihiro Okamura Kazuo Ishii Motohide Nishio Guilherme J. M. Rosa Masahiro Satoh Osamu Sasaki 《Animal Science Journal》2020,91(1)
Direct selection for litter size or weight at weaning in pigs is often hindered by external interventions such as cross‐fostering. The objective of this study was to infer the causal structure among phenotypes of reproductive traits in pigs to enable subsequent direct selection for these traits. Examined traits included: number born alive (NBA), litter size on day 21 (LS21), and litter weight on day 21 (LW21). The study included 6,240 litters from 1,673 Landrace dams and 5,393 litters from 1,484 Large White dams. The inductive causation (IC) algorithm was used to infer the causal structure, which was then fitted to a structural equation model (SEM) to estimate causal coefficients and genetic parameters. Based on the IC algorithm and temporal and biological information, the causal structure among traits was identified as: NBA → LS21 → LW21 and NBA → LW21. Owing to the causal effect of NBA on LS21 and LW21, the genetic, permanent environmental, and residual variances of LS21 and LW21were much lower in the SEM than in the multiple‐trait model for both breeds. Given the strong effect of NBA on LS21 and LW21, the SEM and causal information might assist with selective breeding for LS21 and LW21 when cross‐fostering occurs. 相似文献
75.
Hiroaki Samejima Motohiko Kondo Osamu Ito Takuhito Nozoe Takuro Shinano Mitsuru Osaki 《Soil Science and Plant Nutrition》2013,59(4):545-554
The objective of the present study was to identify the limiting factors in biomass productivity of new tropical rice lines (panicle weight type) by analyzing the relationship between root and shoot activity. Five field experiments using three new lines, IR65598-112-2, IR65600-42-5-2, and IR68544-29-2-1-3-1-2, and one of the highest-yielding indica rice varieties (panicle number type), IR72, were conducted at the International Rice Research Institute from 1997 to 2002. Specific absorption rate of N (SARN , mg N g-1 root dry weight d-1), specific stem sap exudation rate (sap exudation rate, g exudate g-1 root dry weight 12 h-1) and specific root respiratory rate (root respiratory rate, μmol O2 g-1 root dry weight h-1) were determined as indices of root activity. Relative growth rate (RGR, mg g-1 d-1) was used as an index of shoot activity. Compared with IR72, the new lines showed lower RGR and SARN values during the early growth stages. In contrast, during the late growth stages, these activities were higher in the new lines than in IR72. The SARB and sap exudation rate showed a linear correlation with RGR at successive growth stages, regardless of the genotypes and growth conditions. These findings indicated that active root-shoot interaction was maintained throughout the growth period in high-yielding tropical rice, including the new lines and IR72. Therefore, it was considered that SARN and the sap exudation rate are useful indices of root activity regulating RGR in the new lines. However, the root respiratory rate could not be used for selection, because the relationship between RGR and the root respiratory rate changed with the growth conditions. Our findings support the hypothesis that root and shoot activities during maturation are important factors affecting the productivity of the new lines, which have not yet been able to attain the targeted yield. 相似文献
76.
P type humic acid has absorption bands near 615, 570 and 450mµ in the visible region, and is a common component of many soils in north temperate regions (1, 2, 3). Its unusual spectral characteristics are due to a green fraction (Pg), which can be separated from the humic acid dissolved in aqueous alkali by chromatography on Sephadex or cellulose powder, and is precipitated as a reddish-brown substance on acidification (2). In aqueous alkali, it has λmax at 613, 568, 448, 430 (infl.) and 281mµ. The distribution of Pg in different soils is probably related to the presence of fungal sclerotia. The highest content of Pg in p type humic acid is about 10%. We have now obtained evidence that the characteristic colour of Pg is due to the presence of a derivative of 4,9 - dihydroxyperylene - 3,10 - quinone. 相似文献
77.
14C-labelled sodium bicarbonate and 15N-labelled ammonium sulfate were simultaneously vacuum-infiltrated into detached sunflower leaves, and the incorporation of 14C and 15N into free amino acids was chased during 60-min period in the light and in the dark. In the light, the ue specific activity of aspartic acid, alanine, serine and glycine rapidly increased for 5 min and thereafter decreased. On the other hand, that of gultamic acid continued to increase slowly during the entire 60-min period. In the dark, aspartic acid most actively incorporated 14C. The difference of changes in 14C specific activity between glutamic acid and other amino acids was also observed in the dark as in the light. These results suggest that the carbon skeleton of glutamic acid is synthesized from aspartic acid, alanine, serine and glycine. 15N content of glutamine was the highest of all amino acids investigated in the light, and it was followed by glutamic acid. alanine, aspartic acid, serine and glycine, in this order. In the dark, 15N content of glutamic acid fell remarkably and was lower than that of alanine up to 5 min. From these 15N tracer experiments, it is suggested that the incorporation of ammonium into glutamic acid is strictly dependent on light and that alanine incorporates ammonium by the direct amination besides the transamination from glutamic acid. 相似文献
78.
Osamu Sato 《Soil Science and Plant Nutrition》2013,59(4):485-488
P type humic acids showing characteristic absorption maxima at 615, 575, and 450 nm have been found in various types of soils around the world (3, 5, 7,8). P type humic acid was fractionated into a brown fraction (Pb) and a green fraction (Pg) by column chromatography using cellulose powder (4) or Sephadex gel (3). The Pg fraction which has a strong absorption maxima at 615, 575, and 450 nm causes the characteristic absorption pattern of P type humic acid. 相似文献
79.
Akiho KATAYAMA Ayako MIYAZAKI Naohito OKAZAKI Teruko NAKAYAMA Osamu MIKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):931
A total of ten 1–2-year-old rabbits died within 2 weeks at a facility in Ehime prefecture in May 2019. Necropsy revealed liver discoloration and fragility, hemorrhage of some organs and blood coagulation failure. On histopathologic examination, necrotizing hepatitis was a common finding, together with fibrin thrombi in the small vessels and hemorrhage in some organs. Rabbit hemorrhagic disease (RHD) virus gene was detected in liver samples, and viral particles of approximately 32 nm in diameter were found in the cytoplasm of degenerated hepatocytes by electron microscopy. Phylogenetic analysis based on the partial VP60 gene sequence classified it as Lagovirus europaeus GI.2/RHDV2. This is the first confirmed outbreak of RHD caused by globally emerging GI.2/RHDV2 in Japan. 相似文献
80.
Tetrodotoxin--distribution and accumulation in aquatic organisms, and cases of human intoxication
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Many pufferfish of the family Tetraodontidae possess a potent neurotoxin, tetrodotoxin (TTX). In marine pufferfish species, toxicity is generally high in the liver and ovary, whereas in brackish water and freshwater species, toxicity is higher in the skin. In 1964, the toxin of the California newt was identified as TTX as well, and since then TTX has been detected in a variety of other organisms. TTX is produced primarily by marine bacteria, and pufferfish accumulate TTX via the food chain that begins with these bacteria. Consequently, pufferfish become non-toxic when they are fed TTX-free diets in an environment in which the invasion of TTX-bearing organisms is completely shut off. Although some researchers claim that the TTX of amphibians is endogenous, we believe that it also has an exogenous origin, i.e., from organisms consumed as food. TTX-bearing animals are equipped with a high tolerance to TTX, and thus retain or accumulate TTX possibly as a biologic defense substance. There have been many cases of human intoxication due to the ingestion of TTX-bearing pufferfish, mainly in Japan, China, and Taiwan, and several victims have died. Several cases of TTX intoxication due to the ingestion of small gastropods, including some lethal cases, were recently reported in China and Taiwan, revealing a serious public health issue. 相似文献