全文获取类型
收费全文 | 415篇 |
免费 | 13篇 |
国内免费 | 1篇 |
专业分类
林业 | 26篇 |
农学 | 9篇 |
70篇 | |
综合类 | 18篇 |
农作物 | 12篇 |
水产渔业 | 84篇 |
畜牧兽医 | 188篇 |
植物保护 | 22篇 |
出版年
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 15篇 |
2020年 | 6篇 |
2019年 | 4篇 |
2018年 | 11篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 15篇 |
2014年 | 22篇 |
2013年 | 46篇 |
2012年 | 28篇 |
2011年 | 27篇 |
2010年 | 20篇 |
2009年 | 9篇 |
2008年 | 23篇 |
2007年 | 31篇 |
2006年 | 28篇 |
2005年 | 29篇 |
2004年 | 23篇 |
2003年 | 20篇 |
2002年 | 15篇 |
2001年 | 14篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1993年 | 1篇 |
1988年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有429条查询结果,搜索用时 12 毫秒
361.
Gene Defect of Dermatan Sulfate Proteoglycan of Cattle Affected With a Variant Form of Ehlers-Danlos Syndrome 总被引:1,自引:0,他引:1
Motoshi Tajima Sachiko Miyake Kazushige Takehana Ataru Kobayashi Osamu Yamato Yoshimitsu Maede 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(3):202-205
A defect of the core protein of dermatan sulfate proteoglycan was suspected in a Holstein calf affected with a variant form of Ehlers-Danlos syndrome. The mutation was a guanine-to-adenine transition at nucleotide position 254, which resulted in a serine-to-asparagine substitution of the bovine proteoglycan core protein. This substitution occurred in the serine-glycine dipeptide repeat that was suspected to be the binding portion of dermatan sulfate. This point mutation in the genome was also detected by the use of restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) methods. The results of the RFLP and PCR indicated that the calf was a heterozygote of an abnormal gene and a normal gene of the core protein. The interpretation of these data revealed that the functional abnormality in cutaneous tissues of the calf was caused by an abnormal gene of the proteoglycan core protein, which induced a substitution of amino acid. 相似文献
362.
Eiji NAITO Kohei NAKATA Hiroki SAKAI Osamu YAMATO Md Shafiqul ISLAM Sadatoshi MAEDA Hiroaki KAMISHINA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):199
Canine degenerative myelopathy (DM) is a progressive neurodegenerative disease of the spinal cord. The diagnosis is based on the observation of clinical signs, genetic testing, and exclusion of other spinal cord diseases, and a definitive diagnosis of DM can only be confirmed by postmortem histopathological findings. The aim of this study was to investigate the diagnostic ability of diffusion tensor imaging (DTI) for DM. Eight DM-affected Pembroke Welsh Corgis, thirteen dogs with thoracolumbar intervertebral disk herniation (IVDH), and six healthy control dogs were included. All dogs were scanned using a 3.0-T MRI system. Apparent diffusion coefficient (ADC) and fractional anisotropy (FA) values were calculated for each intervertebral disk level slice between T8–T9 and L2–L3 intervertebral disk levels, and the entire area of the thoracolumbar spinal cord between T8–T9 and L2–L3 intervertebral disk levels (T8–L3 region). The ADC and FA values of the T8–L3 region were significantly lower in the DM group than in the IVDH group. The ADC values for the T8–L3 region had a moderate negative correlation with clinical duration (rs= −0.723, P=0.043); however, the FA values of other intervertebral disk levels and T8–L3 region had no correlation with clinical durations. The measurement of DTI indices can be used to quantitatively assess neurodegeneration and may have diagnostic value for DM. In particular, the ADC value of the T8–L3 region may aid in making a non-invasive premortem diagnosis of DM. 相似文献
363.
364.
Deng H Grunder S Cordova KE Valente C Furukawa H Hmadeh M Gándara F Whalley AC Liu Z Asahina S Kazumori H O'Keeffe M Terasaki O Stoddart JF Yaghi OM 《Science (New York, N.Y.)》2012,336(6084):1018-1023
We report a strategy to expand the pore aperture of metal-organic frameworks (MOFs) into a previously unattained size regime (>32 angstroms). Specifically, the systematic expansion of a well-known MOF structure, MOF-74, from its original link of one phenylene ring (I) to two, three, four, five, six, seven, nine, and eleven (II to XI, respectively), afforded an isoreticular series of MOF-74 structures (termed IRMOF-74-I to XI) with pore apertures ranging from 14 to 98 angstroms. All members of this series have noninterpenetrating structures and exhibit robust architectures, as evidenced by their permanent porosity and high thermal stability (up to 300°C). The pore apertures of an oligoethylene glycol-functionalized IRMOF-74-VII and IRMOF-74-IX are large enough for natural proteins to enter the pores. 相似文献
365.
促性腺激素(LH,FSH)对鸡卵泡孕酮分泌的影响 总被引:3,自引:0,他引:3
摘出垂体12 h 后的卵泡孕酮(P_4)浓度显著下降.单独添加 LH 时第一卵泡(F_1)的P_4浓度显著增加,同时添加 LH 和 FSH 时,所有卵泡的颗粒层 P_4浓度恢复到对照的水平.但是单独添加 FSH 时无促进作用.LH 和 FSH 的处理对卵泡的卵泡膜 P_4浓度影响极小.在细胞培养液中添加 LH 和 FSH 可以促进颗粒层的 P_4合成,这种反应以 F_1最为明显.LH 和 FSH同时添加时对颗粒层 P_4合成有显著效果,但是对卵泡膜 P_4合成无明显促进作用.表明 P_4在 LH和 FSH 的协同调控下,主要在 F_1的颗粒层中合成. 相似文献
366.
Susumu Kurinobu Daryono Prehatin Naiem Mohanmad Kenji Matsune Osamu Chigira 《Journal of Forest Research》2007,12(3):230-236
A growth model for a plantation of Paraserianthes falcataria was provisionally derived from measurements of 32 permanent plots taken over a period of 2 years in Pare, Indonesia. This
model first predicts height growth with the polymorphic site index equation. Then density-related growth and mortality, that
is, diameter and stand density, are calculated to satisfy the mathematical relationship for the reciprocal and self-thinning
equations, both of which were based on the maximum size–density line with the slope of −1.759 identified in this study. Cumulative
predictions on diameter and stand density, starting with each of three age classes, 3, 4, and 5 years after planting, and
continuing for the next 2 years, agreed well with the observations of age-class mean. An exception was for stands that were
7 years old, which were marginal, but closest to the rotation age of 8 years. Contrasting height/diameter relationships among
the three age classes were described reasonably well with this model, and the predicted basal growth area was found to agree
fairly well with observations. These results suggest that the growth model presented here has good potential for applying
size–density control for plantations of P. falcataria. However, measurements for an additional few years might be necessary to derive a model that will more accurately predict
diameter size at the rotation age. 相似文献
367.
Yamada O Kobayashi M Sugisaki O Ishii N Ito K Kuroki S Sasaki Y Isotani M Ono K Washizu T Bonkobara M 《Veterinary immunology and immunopathology》2011,142(1-2):101-106
Target therapy using the tyrosine kinase inhibitor imatinib is one of the new therapeutic approaches for canine mast cell tumors (MCTs). In the present report, we demonstrate a clinical response to imatinib in a dog with MCT carrying a c-kit c.1523A>T mutation. Moreover, the effect of this mutation on the phosphorylation status of KIT and the inhibitory potency of imatinib on the phosphorylation of the mutant KIT were examined in vitro. A dog with a MCT tumor mass on the right forelimb sole with lymph node metastasis and mastocytemia was treated with imatinib. The MCT mass markedly shrank and mastocytemia became undetectable with 2 weeks of treatment. The lymph node enlarged by metastasis became normal in size with 5 weeks of treatment. From the sequencing analysis of c-kit in tumor cells, a substitution mutation c.1523A>T that alters the amino acid composition (p.Asn508Ile) within the extracellular domain of KIT was identified. The mutant KIT expressed on 293 cells showed ligand-independent phosphorylation and imatinib suppressed this phosphorylation in a dose-dependent manner. From these findings, imatinib was considered to elicit a clinical response in a canine case of MCT via inhibition of the constitutively activated KIT caused by a c-kit c.1523A>T mutation. 相似文献
368.
Seiji TSUGE Mami NISHIDA Ayako FURUTANI Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(3):215-223
A genomic library of Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174 was screened for 4-methylumbelliferyl β-D-glucoside-hydrolyzing (MUGase) activity. In subcloning of one of the MUGase-positive clones, an approximately 4.2-kb SacI-SphI fragment conferred not only MUGase activity but also 4-methylumbelliferyl β-D-cellobioside-hydrolyzing (MUCase) activity. Sequence analysis showed that the fragment contained an ORF of 2951 bp. The conceptual
ORF product was significantly homologous with 1,4-β-D-glucan glucohydrolase D (CELD) from Pseudomonas fluorescens subsp. cellulosa, and was named CELDXo. Cell fractionation experiments suggested that CELDXo is localized in the cell-envelope fraction. We constructed a CELDXo-deficient mutant (74ΔCELD) from X. o. pv. oryzae. Little MUCase activity was detected in the cell-envelope fraction prepared from the mutant. The mutant 74ΔCELD did not grow
in synthetic medium containing cellobiose as the sole sugar source. On the other hand, growth in rice leaves and pathogenicity
of the mutant and the parental strain did not differ. These results suggested that CELDXo is involved in cellobiose utilization of X. o. pv. oryzae but that the gene is not required for bacterial growth in rice leaves.
Received 16 February 2001/ Accepted in revised form 11 April 2001 相似文献
369.
Go KAWAHARA Yuta TAKAYAMA Makoto SUGIYAMA Hiromi IKADAI Osamu HASHIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(10):1410
Here, we report details of a new infectious disease in wild-caught Japanese fire-bellied newts (Cynops pyrrhogaster), a Near Threatened species. Skin lesions consisting of numerous masses were found in the animals near Lake Biwa, Shiga Prefecture, Japan. The gross appearance of the skin lesions showed blister-, cyst-, and/or tumor-like morphology. Various sizes of skin lesions were observed on their entire body surface. Histologically, spherical basophilic cysts, including numerous spores, were observed in the dermis layer. Ultrastructural analysis indicated the presence of main bodies of flagellated zoospores within the spores. While 18s rRNA gene sequencing indicated that the skin lesions were due to dermocystid infection. To our knowledge, this is the first report of dermocystid infection in this amphibian in Japan. Further studies are needed to prevent epidemics and to establish diagnostic and treatment methods. 相似文献
370.
Osamu Yada Mutsuyosi Tsuchimoto Qin Wang Paula Andrea Gomez Apablaza Abdul Jabarsyah Katsuyasu Tachibana 《Fisheries Science》2001,67(5):948-955
ABSTRACT: To clarify the cause of differences in the temporal change of K -value among fish species living in the same habitat water temperature, the influence of the interposition of pink muscle fibers into dorsal ordinary muscle on 5'-inosine monophosphate (5'-IMP) degrading activity was examined. Fourteen fish species from the Sakishima Islands (habitat water temperature 28°C) and from Nagasaki (habitat water temperature 17°C) were used for the sample fishes. Each of 5'-IMP and ρ-nitrophenol phosphate (ρ-NPP) degrading activities showed a peak at near pH 8.0 and near pH 5.0, respectively. These activities were somewhat higher in fish from Nagasaki than in fish from the Sakishima Islands. The interposition percentage of pink muscle fibers into dorsal ordinary muscle correlated significantly ( P < 0.05) with the 5'-IMP degrading activity at pH 7.0. Furthermore, the activity at pH 7.0 correlated significantly ( P < 0.001) with the increasing rate of K -value at 32°C (ΔK32 ). These results suggest that the difference in ΔK32 among fish species in the same habitat water temperature might be caused by differences in 5'-IMP degrading activity because of the differences in the interposition rates of pink muscle fibers into dorsal ordinary muscle. 相似文献