Traits in Spring Wheat Cultivars Associated with Yield Loss Caused by a Heat Stress Episode after Anthesis

Heat stress resulting from climate change and more frequent weather extremes is expected to negatively affect wheat yield. We evaluated the response of different spring wheat cultivars to a post‐anthesis high temperature episode and studied the relationship between different traits associated with heat tolerance. Fifteen spring wheat (Triticum aestivum L.) cultivars were grown in pots under semifield conditions, and heat stress (35/26 °C) and control treatments (20/12 °C) were applied in growth chambers for 5 days starting 14 days after flowering. The heat stress treatment reduced final yield in all cultivars. Significant variation was observed among cultivars in the reduction in average grain weight and grain dry matter yield under heat stress (up to 36 % and 45 %, respectively). The duration of the grain‐filling period was reduced by 3–12 days by the heat treatment. The reduction in the grain‐filling period was negatively correlated with grain nitrogen yield (r = ?0.60). A positive correlation (r = 0.73) was found between the treatment effect on green leaf area (GLA) and the reduction in yield resulting from heat stress. The amount of stem water‐soluble carbohydrates (WSC) was not related to treatment effects on grain yield or grain weight. However, the treatment effect on stem WSC remobilization was negatively correlated with reduction in grain‐filling duration due to heat stress (r = ?0.74) and positively with treatment effect on grain N yield (r = 0.52). The results suggest that the effect of the heat treatment on GLA was the trait most associated with yield reduction in all cultivars. These findings suggest the importance of ‘stay green’‐associated traits in plant breeding as well as the need for better modelling of GLA in crop models, especially with respect to brief heat episodes during grain filling. There is in particular a need to model how heat and other stresses, including interacting effects of heat and drought, affect duration of GLA after flowering and how this affects source–sink relations during grain filling.     

Design of an Organic Farming Crop-Rotation Experiment

A field experiment is being conducted which focuses on crop rotations for cereal production in organic farming. The objective of the experiment is to explore the possibilities for both short-term and long-term increases in organic cereal production through manipulation of crop-rotation design on different soil types. This paper describes the design of a rather complex experiment, and later papers will describe and discuss the results. Three factors are included in the experiment in a factorial design with two replicates: (1) fraction of grass-clover and pulses in the rotation (crop rotation); (2) catch crop (with or without catch crop or bi-cropped clover); and (3) manure (with or without animal manure applied as slurry). All fields in all rotations are represented in each year. The experimental factors are defined to allow management to be adjusted for optimization of the individual treatment combinations. This makes the systems more realistic and the results more applicable in practical farming. The experiment is being conducted at four locations representing major soil types and climate regions in Denmark. The main design criteria are related to requirements for a long-term experiment and the need for performing studies and experiments within the experiment itself.     

Nitrate leaching from organic arable crop rotations: effects of location, manure and catch crop

Abstract. Nitrate leaching from crop rotations supporting organic grain production was investigated from 1997 to 2000 in a field experiment at three locations in Denmark on different soil types. Three experimental factors were included in the experiment in a factorial design: (1) proportion of N₂-fixing crops in the rotation (crop rotation), (2) catch crop (with and without), and (3) manure (with and without). Three, four-course rotations were compared, two at each location. The nitrate leaching was measured using ceramic suction cells. Leaching losses from the crop rotation with grass–clover green manure and without catch crops were 104, 54 and 35 kg N ha⁻¹ yr⁻¹ on the coarse sand, the loamy sand, and the sandy loam, respectively. There was no effect of manure application or time of ploughing-in the grass–clover green manure crop on the accumulated nitrate leaching from the entire rotation. Catch crops reduced nitrate leaching significantly, by 30–38%, on the sandy soils. At all locations catch crops reduced the annual averaged nitrate concentration to meet drinking water quality standards in the crop rotation with green manure. On the coarse sand there was a time lag between the onset of drainage and the start of N-uptake by the catch crop.     

Modelling development of broccoli (Brassica oleracea L. var. italica) from transplanting to head initiation

Summary

A model is suggested to predict head initiation in broccoli. Head initiation is defined as the time when the apex diameter reaches 0.6 mm and predictions are made from observations of air temperature. The model is constructed from three years of field experiments with three cultivars and four plantings per year. No juvenile phase after transplanting could be estimated and the model consists only of a head induction phase starting at transplanting. The rate of head induction is modelled by a piecewise linear temperature response function with a base, optimum, and maximum temperature. At optimum temperature the duration of the head induction phase is shortest. The estimates for base, optimum, and maximum temperature are 9.98C, 16.18C and 22.38C, respectively. The estimates for cultivar dependent thermal time requirements are 49 day-degrees, 55 day-degrees, and 38 day-degrees for cvs Caravel, Shogun and Emperor, respectively. The three cultivars were anticipated to have the same cardinal temperatures for head induction. The model of the head induction phases can account for 68% of the variation in the observed durations from transplanting to head initiation. A model of leaf appearance is used to standardize transplant size to four visible leaves at planting. The leaf appearance rate is described by a linear relationship to number of leaves and air temperature and the model can account for 89% of the variation in time from transplanting to appearance of a certain number of leaves. The temperature response of leaf appearance rate had a base temperature of about 2 to 38C, depending on cultivar, and showed no indications of having a temperature optimum below 208C. The leaf appearance rate at 158C range from 0.2 leaves per day for plants with four visible leaves to 0.4 leaves per day for plants with 14 visible leaves.     

Health screening for a translocation of captive-reared tuatara (Sphenodon punctatus) to an island refuge

AIMS: To screen tuatara undergoing translocation from a captive crèche to an island refuge for evidence of health and known diseases, and apply basic epidemiological techniques to assess the significance of disease test results.

METHODS: Tuatara (n=353) were physically examined and samples were taken from a random selection (n=30) for estimated white cell counts, screening for haemoparasites, and culture for Salmonella, Yersinia, Aeromonas and Campylobacter spp. Direct faecal smears were carried out on-site, and faecal floats were later performed to assess levels of endoparasitism with helminths and protozoa (n=69). Modified Ziehl-Neelsen staining was used to screen faecal smears, and positive specimens were further screened using an immunofluorescence antibody (IFA) test for Cryptosporidium oocysts.

RESULTS: There was no evidence of external parasites on any of the animals examined and only one animal had a gross abnormality. All estimated white cell counts were in the range 2.8– 17.5 × 10⁹/L. No haemoparasites were observed. There were no enteric pathogens cultured, indicating the intestinal carriage of these bacteria in the tuatara was 9.4%. Of the 69 individual faecal samples examined, 12 (17%) had unidentified coccidial oocysts, 21 (30%) had nematode ova of various kinds, and 12 (17%) had intestinal carriage of motile protozoa consistent with Trichomonas spp and another unidentified organism. Nineteen (28%) tuatara had acid-fast oocysts present; however, IFA staining failed to detect any Cryptosporidium oocysts.

CONCLUSIONS: Our understanding of the diversity of gastrointestinal endoparasites affecting tuatara is inadequate as many of the parasite ova seen could not be identified. This is the first record of tuatara as a host for Trichomonas spp of protozoa in the gastrointestinal tract.     

Dermatomycosis caused by Paranannizziopsis australasiensis in five tuatara (Sphenodon punctatus) and a coastal bearded dragon (Pogona barbata) in a zoological collection in New Zealand

CASE HISTORY: Health monitoring of tuatara (Sphenodon punctatus) at Auckland Zoo between 2001 and 2009 showed that 58/93 tuatara had been affected by dermatitis of unknown origin. From 2011 onwards, cases of suspected fungal dermatitis underwent extensive diagnostic investigations.

CLINCAL FINDINGS: Six cases of dermatomycosis were attributed to Paranannizziopsis australasiensis, five in tuatara and one in a coastal bearded dragon (Pogona barbata). Cases presented typically as raised, yellow to brown encrustations on the skin. Severe cases progressed to necrotising ulcerative dermatitis, and in the bearded dragon to fatal systemic mycosis. Following topical and systemic treatments, lesions resolved in all five tuatara.

LABORATORY FINDINGS: Histopathological examination of skin biopsy samples revealed dermatitis with intralesional septate branching hyphae. Fungal culture yielded isolates morphologically resembling Chrysosporium species, and isolates were submitted for molecular confirmation and sequencing of DNA.

DIAGNOSIS: All six cases were confirmed as dermatitis due to infection with P. australasiensis, on the basis of fungal culture and DNA sequencing of isolates.

CLINICAL RELEVANCE: These are the first reported cases of dermatomycosis associated with P. australasiensis infection in tuatara, and the first cases in which systemic therapeutic agents have been used in the treatment of such disease. Tuatara at the Auckland Zoo are now routinely examined every 3 months and tissue samples from any lesions sent for histopathology and fungal culture. Further work to elucidate the epidemiology and significance of P. australasiensis infections in reptiles in New Zealand is important for both welfare and conservation purposes.     

Anti‐Müllerian Hormone in the Domestic Dog during the Anestrus to Oestrous Transition

The reproductive cycle of the domestic dog features a long period of relative ovarian inactivity or anestrus. The mechanism of anestrous termination/oestrous resumption is not yet fully understood, which presents a challenge to the development of oestrous induction protocols. In this study, we assess the possibility that anti‐Müllerian hormone (AMH) might play a role in this transition by characterizing its patterns of expression in the circulation during the transition from anestrus to oestrous and in all stages of ovarian follicular growth. Serum samples from five beagles (2.0–4.5 years) were collected three times per week at least 30 days prior to the onset of oestrous and assessed for AMH concentrations. Serum AMH concentration increased significantly during the transition from anestrus to proestrus and then declined back to the anestrous baseline beginning on day ?4 before the luteinizing hormone surge, which was determined by changes in serum progesterone concentrations. Cortical sections of ovaries from females undergoing routine ovariohysterectomy (aged 8 months–5 years, n = 4) were evaluated for AMH by immunohistochemistry. Pre‐antral and small antral follicles were most strongly immunoreactive for AMH. These data suggest that the increase in the number of antral follicles is associated with the rise in serum AMH as the anestrous period comes to an end. The rise in AMH might be useful in predicting the onset of oestrus and therefore assist with the optimization of oestrous induction protocols and possibly other assisted reproductive technologies.     

X-linked spondylo-epiphyseal dysplasia tarda in the Danish-Swedish farm hound

In two litters from the same parents, three out of four males had an abnormally short leg and body length. Affected dogs showed signs of pain when moving, which could be eliminated by analgesia. On radiography, these animals had widened, radiolucent, irregularly bordered intervertebral disc spaces. When examined at seven months of age, the epiphyses appeared widened and irregular in shape and outline. General bone opacity in the vertebral column was lower in the affected male dogs than in the normal littermate. The affected dogs developed spondylosis and arthrosis of the larger limb joints. All affected dogs were euthanased on humane grounds, the eldest at the age of two years nine months. Based on the clinical and radiographic evidence, the condition seen in the male dogs described here resembles X-linked spondylo-epiphyseal dysplasia tarda caused by a collagenopathy due to malformation of COL2A1 as seen in human beings.     

Subsoil loosening in a crop rotation for organic farming eliminated plough pan with mixed effects on crop yield

Compacted subsoil may reduce plant root growth with resulting effects on plant uptake of water and nutrients. In organic farming systems subsoil loosening may therefore be considered an option to increase nutrient use. We investigated the effect of subsoil loosening with a paraplow to ca. 35 cm depth within a four-crop rotation in an organic farming experiment at Foulum (loamy sand) and Flakkebjerg (sandy loam) in Denmark. In each of the years 2000–2003, half of four plots per site were loosened in the autumn bearing a young grass-clover crop (mixture of Lolium perenne L., Trifolium repens L. and Trifolium pratense L.) established by undersowing in spring barley (Hordeum vulgare L.). The grass-clover was grown for another year as a green manure crop and was followed by winter wheat (Triticum aestivum L.), lupin (Lupinus angustifolius L.):barley and spring barley in the following 3 years. On-land ploughing was used for all cereal and pulse crops. Penetration resistance was recorded in all crops, and the results clearly showed that subsoil loosening had effectively reduced the plough pan and that the effect lasted at least for 3.5 years. Measurements of wheat root growth using minirhizotrons at Foulum in 2002/2003 did not show marked effects of subsoil loosening on root frequency in the subsoil. Subsoil loosening resulted in reduced growth and less N uptake of the grass-clover crop in which the subsoil loosening was carried out, probably due to a reduced biological nitrogen (N) fixation resulting from a smaller clover proportion. This had a marked effect on the growth of the succeeding winter wheat. Negative effect of subsoil loosening on yield of winter wheat and spring barley was observed without manure application, whereas small positive yield effect of subsoil loosening was observed in crops with a higher N supply from manure. Yield decrease in winter wheat was observed in years with high winter rainfall. There was no significant effect of subsoiling on grain yield of the lupin:barley crops, although subsoiling had a tendency to increase crop growth and yield during dry summers. Our results suggest that subsoil loosening should not be recommended in general under Danish conditions as a measure to ameliorate subsoil compaction.