Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity

Annexins belong to an evolutionarily conserved multigene family of proteins expressed throughout the animal and plant kingdoms. Although they are soluble cytosolic proteins that lack signal sequences, they have also been detected in extracellular fluids and have been associated with cell surface membranes, where they could be involved in anti-haemostatic and anti-inflammatory functions. Schistosome annexins have been identified on the parasite's tegument surface and excretory/secretory products, but their functions are still unknown. Here we report the cloning, sequencing, in silico analysis, and functional characterization of a Schistosoma bovis annexin. The predicted protein has typical annexin secondary and tertiary structures. Bioassays with the recombinant protein revealed that the protein is biologically active in vitro, showing fibrinolytic and anticoagulant properties. Finally, the expression of the native protein on the tegument surface of S. bovis schistosomula and adult worms is demonstrated, revealing the possibility of exposure to the host's immune system and thus offering a potential vaccine target for the control of schistosomiasis in ruminants.     

Biological control of goat gastrointestinal helminthiasis by Duddingtonia flagrans in a semi-arid region of the northeastern Brazil

The aim of this study was to test a pellet formulation in a sodium alginate matrix of Duddingtonia flagrans in the biological control of goat gastrointestinal helminths kept in a native pasture in a semi-arid region of Paraíba state, northeastern Brazil. An area of 2.4 ha was divided into three paddocks, where groups of seven goats ware formed. Each group received the following treatments during the months of March to August 2011: D. flagrans group, received 3g of pellets containing D. flagrans (AC001) for each 10 kg/l.w., twice a week; Moxidectin 0.2% group, received 0.2mg/kg of Moxidectin 0.2% orally, every 30 days; Control group, received 3g of pellets without fungi per 10 kg/l.w., twice a week. Each month, a tracer goat was placed in each group for 30 days and then sacrificed and necropsied. The D. flagrans group showed a greater reduction in EPG, increased weight gain, higher rates of packed cell volume and lower parasitic load burden in the tracer goats compared to Moxidectin 0.2% and Control groups. D. flagrans was efficient in controlling goat gastrointestinal helminthiasis in a semi-arid region of northeastern Brazil.     

Study of Toxoplasma infection in Brazilian wild mammals: serological evidence in Dasypus novemcinctus Linnaeus, 1758 and Euphractus sexcinctus Wagler, 1830

Toxoplasmosis is a worldwide distributed zoonosis that affects man and most warm-blooded animals, with a great economic impact in animal and public health. Serum samples from nine 9-banded armadillos, three 6-banded armadillos, three coatimundis, two opossums and one nutria were submitted for anti-Toxoplasma gondii antibody detection by means of a modified direct agglutination method. Encephalic tissue of three 6-banded armadillos, one 9-banded armadillo, one coatimundi and one nutria were digested in acid pepsin solution and inoculated into Swiss mice for parasite isolation. Only one serum sample from a nine-banded armadillo and two from six-banded armadillos reacted producing titers equal to 256, 512 and 512, respectively. T. gondii was isolated in two 6-banded armadillos, one of which was not positive in the serological test.     

First report of a phytoplasma associated with Spondias purpurea (Jocote de Corona) in El Salvador

A new disease of the fruit tree Jocote de Corona (Spondias purpurea L.) was observed in El Salvador, Central America. The symptoms included small chlorotic leaves, highly proliferating shoots, and shortened internodes. Absence of sweet pulp in fruits made them inedible, causing considerable yield losses for farmers. The disease etiology was investigated using polymerase chain reaction with phytoplasma-specific primers, sequencing, and phylogenetic analysis. We obtained no amplification products from symptomless plants, whereas all tests were positive from plants with symptoms. Phylogenetic analysis indicated that this phytoplasma clustered in the 16S rIII group, the type member of which is X-disease phytoplasma. This is the first report of a phytoplasma associated with Jocote de Corona disease in El Salvador and Central America. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AJ888471     

Matricaria chamomilla CH12 decreases handling stress in Nelore calves

Matricaria chamomilla CH₁₂ is a phytotherapeutic or homeopathic product, which has been used to reduce stress. Here, we examined its effect on preventing handling stress in bovines. Sixty Nelore calves were randomly distributed into two equal groups. One group was administered Matricaria chamomilla CH₁₂ in diet and the other the ''control'' was not. Animals in both groups were maintained unstressed for 30 days to adjust to the feeding system and pasture, and were then stressed by constraint on the 31th, 38th, 45th and 60th experimental days. Blood samples were taken on these days after animals had been immobilization in a trunk contention for 5 min. Stress was followed by analyzing serum cortisol levels. These peaked on the 45th day and then decreased, but not to baseline, on the 60th day. On the 45th day cortisol levels were significantly lower in animals fed Matricaria chamomilla CH₁₂, suggesting that this product reduces stress. These effects may be a consequence of its inhibiting cortisol production and its calming and anxiolytic effects.     

ULTRASONOGRAPHIC APPEARANCE OF OVARIAN TUMORS IN 10 DOGS

Signalment, clinical history, physical examination, clinicopathologic, readiographic and ultrasonographic findings of 10 female dogs with histologically confirmed ovarian neoplasms were reviewed. Ultrasonographic images and reports were reviewed for (1) location, size, outer margins, and echogenicity of the mass(es), (2) presence of free abdominal fluid, (3) evidence of uterine abnormalities, and (4) signs of metastatic disease. The masses were classified according to their ultrasonographic pattern in solid, solid with cystic component, and cystic. The masses were ultrasonographically reported as being of ovarian origin in eight dogs, and this origin was included in the list of differentials in the remaining two dogs. When present, abdominal effusions and uterine abnormalities were diagnosed by means of ultrasound.     

A comparison of potato seed tuber yields in beds,pots and hydroponic systems

The objective of this study was to compare potato seed tuber production of cvs. Monalisa and Agata growing in beds, pots or hydroponics, with either single or staggered harvests. All culture systems were established in plastic sheeting-covered greenhouses protected with an anti-aphid network. The beds and 3 L pots were filled with Plantmax^® substrate and placed in suspended beds. The hydroponic system utilized NFT (Nutrient film technique) and 4 m × 15 cm × 7 cm PVC tubes with a 4% slope and the fertilizers were a commercial formula. Each experiment was 4 treatments in factorial Scheme 2 × 2 with 2 cultivars (Monalisa and Agata) and 2 harvest methods (single and staggered). All three experiments were carried out in randomized design with 6 replicates and 7 plants per replicate.     

Biogenic calcium phosphate transformation in soils over millennial time scales

Background, aim, and scope  Changes in bioavailability of phosphorus (P) during pedogenesis and ecosystem development have been shown for geogenic calcium phosphate (Ca-P). However, very little is known about long-term changes of biogenic Ca-P in soil. Materials and methods  Long-term transformation characteristics of biogenic Ca-P were examined using anthropogenic soils along a chronosequence from centennial to millennial time scales. Results and discussion  Phosphorus fractionation of Anthrosols resulted in overall consistency with the Walker and Syers model of geogenic Ca-P transformation during pedogenesis. The biogenic Ca-P (e.g., animal and fish bones) disappeared to 3% of total P within the first ca. 2,000 years of soil development. This change concurred with increases in P adsorbed on metal-oxides surfaces, organic P, and occluded P at different pedogenic time. Phosphorus K-edge X-ray absorption near-edge structure (XANES) spectroscopy revealed that the crystalline and therefore thermodynamically most stable biogenic Ca-P was transformed into more soluble forms of Ca-P over time. While crystalline hydroxyapatite (34% of total P) dominated Ca-P species after about 600–1,000 years, β-tricalcium phosphate increased to 16% of total P after 900–1,100 years, after which both Ca-P species disappeared. Iron-associated P was observable concurrently with Ca-P disappearance. Soluble P and organic P determined by XANES maintained relatively constant (58–65%) across the time scale studied. Conclusions  Disappearance of crystalline biogenic Ca-P on a time scale of a few thousand years appears to be ten times faster than that of geogenic Ca-P.     

Tree Adoption and Nursery and Propagation Practices in Smallholder Upland Farms in Inopacan and Isabel, Leyte, the Philippines

This paper examines seedling nursery and propagation practices in smallholder farms in two upland areas in Leyte in the Philippines. The case study areas are relatively remote, little forestry extension has been carried out, and traditional practices can be observed. These practices were examined through a survey of smallholders and observation of forestry activities. It was found that in spite of various constraints and notably the lack of planting materials, farmers are interested in forestry and are adopting some naturally growing trees and producing their own seedlings. To make way to their crops, farmers have removed some naturally growing trees of some particular species on their farms. For the purpose of introducing trees to farms, wildlings are widely used as planting material. Some farmers also collect seeds and treat them in preparation for planting, using methods which differ from those recommended elsewhere by extension agencies, but which are nevertheless highly effective.