Response of White Leghorn and Nigerian cockerels to experimental salmonella infection

Summary Twenty-two Nigerian cockerels and 19 White Leghorn cockerels were each injected intramuscularly with 1·5×10⁹ viable organisms of a relatively avirulent strain ofSalmonella gallinarum (NCTC 10532). The Nigerian cockerels were more resistant to infection as judged by the values of the haemoglobin, haematocrit, red cell count, leucocyte count and antibody titre during the course of infection. The authors suggest that the higher resistance may be due to the earlier growth and higher organ weight of the bursa of Fabricius of the Nigerian breed than of the White Leghorn (Aire,1973).

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Sumario Ventidos pollos Nigerianos y diecinueve White Leghorn fueron inoculados individualmente vía intramuscular, con 1·5 × 10⁹ de una suspensión relativamente avirulenta de una cepa deSalmonella gallinarum (NCTC 10532). Los pollos Nigerianos fueron más resistentes a la infección, teniendo en cuenta los valores de hemaglobina, hematocrito, número de globulos rojos, leucocitos y título de anticuerpos durante el curso de la infección. Los autores sugieren, que la mayor resistencia de la raza Nigeriana, podría estar relacionada con el peso y desarrollo mástemprano de la bolsa de Fabricius.

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Résumé Trente deux coquelets Nigériens et dix neuf coquelets Leghorn blancs ont été infectés, chacun par une injection intramusculaire de 1·5 × 10⁹ germes vivants d'une souche relativement avirulente deSalmonella gallinarum (NCTC 10532). L'examen, tout au long de l'expérience, de l'hémoglobine, des hématocrites, la numération des hématies et des leucocytes et le titrage des anticorps ont montré que les coquelets Nigériens étaient plus résistants. Les auteurs suggèrent que cette résistance supérieure peut être due au développement plus précoce et au poids supérieur de la bourse Fabricius chez le coquelet Nigérien par rapport au coquelet Leghorn blanc (Aire, 1973).

     

In vitro evaluation of a custom cutting jig and custom plate for canine tibial plateau leveling

OBJECTIVE: To design and manufacture custom titanium bone plates and a custom cutting and drill guide by use of free-form fabrication methods and to compare variables and mechanical properties of 2 canine tibial plateau leveling methods with each other and with historical control values. SAMPLE POPULATION: 10 canine tibial replicas created by rapid prototyping methods. PROCEDURES: Application time, accuracy of correction of the tibial plateau slope (TPS), presence and magnitude of rotational and angular deformation, and replica axial stiffness for 2 chevron wedge osteotomy (CWO) methods were assessed. One involved use of freehand CWO (FHCWO) and screw hole drilling, whereas the other used jig-guided CWO (JGCWO) and screw hole drilling. RESULTS: Replicas used for FHCWO and JGCWO methods had similar stiffness. Although JGCWO and FHCWO did not weaken the replicas, mean axial stiffness of replicas after JGCWO was higher than after FHCWO. The JGCWO method was faster than the FHCWO method. Mean +/- SD TPS after osteotomy was lower for FHCWO (4.4 +/- 1.1 degrees ) than for JGCWO (9.5 +/- 0.4 degrees ), and JGCWO was more accurate (target TPS, 8.9 degrees ). Slight varus was evident after FHCWO but not after JGCWO. Mean postoperative rotation after JGCWO and FHCWO did not differ from the target value or between methods. CONCLUSIONS AND CLINICAL RELEVANCE: The JGCWO method was more accurate and more rapid and resulted in more stability than the FHCWO method. Use of custom drill guides could enhance the speed, accuracy, and stability of corrective osteotomies in dogs.     

Interaction of photoperiod and temperature in the control of growth and dormancy of Prunus species

Growth and dormancy induction of seedlings or micropropagated plants of three Prunus species were studied under controlled environment conditions. All the species tested, P. cerasus L. and P. insititia L. (two cultivars each), and P. avium L. were insensitive to photoperiod at high temperature and maintained continuous growth in both 10 and 24-h photoperiods at 21 °C. At lower temperatures, however, growth was controlled by the interaction of photoperiod and temperature, the species and cultivars varying somewhat in their responses. At 9 °C growth cessation was induced regardless of day-length conditions in the plum rootstocks ‘St. Julien A’ and ‘Weito’ as well as in the sour cherry rootstock ‘Weiroot’, whereas in the sour cherry rootstock ‘Gisela 5’ growth cessation took place in short day (SD) only. At intermediate temperatures (12 and 15 °C) growth cessation occurred in SD only in both sour cherry cultivars. In P. avium seedlings on the other hand, growth cessation in SD was only induced at 9 °C, continuous but reduced growth taking place also in SD at all higher temperatures. Growth rates increased progressively with increasing temperature under long day (LD) conditions in all species, and this was associated with increased internode length in LD compared with SD conditions. Production of new leaves was unaffected by photoperiod at high temperature, but was higher in LD than in SD at lower temperatures. After growth cessation at low temperature the plants developed winter buds and became dormant also in LD conditions. These results demonstrate that, like several species of the Pomoidae subfamily of the Rosaceae, these Prunus species are insensitive to short photoperiods at relatively high temperatures. However, the photoperiodic response of the Prunus species is highly temperature dependent, and the transition temperatures for shifts in the photoperiodic response mode vary among the species.     

Interaction of short day and timing of nitrogen fertilization on growth and flowering of ‘Korona’ strawberry (Fragaria × ananassa Duch.)

The effects of timing of nitrogen (N) fertilization relative to the beginning of a 4-week floral-inducing short-day (SD) period have been studied in ‘Korona’ strawberry plants under controlled environment conditions. Groups of low fertility plants were fertilized with 100 ml of calcium nitrate solution for 3 days a week for a period of 3 weeks starting at various times before and at the beginning of the SD period, as well as at different times during the SD period. All plants, including SD and long day (LD) control plants, received a weekly fertilization with a low concentration complete fertilizer solution throughout the experiment. Leaf area, fresh and dry matter increments of leaves, crowns and roots, as well as leaf chlorophyll concentration (SPAD values) were monitored during the experimental period. A general enhancement of growth took place at all times of N fertilization. This was paralleled by an increase in leaf chlorophyll concentration, indicating that the control plants were in a mild state of N deficiency. When N fertilization was started 2 weeks before beginning of the SD period, flowering was delayed by 7 days, and this was gradually changed to an advancement of 8 days when the same treatment was started 3 weeks after the first SD. The amount of flowering was generally increased by N fertilization although the effect varied greatly with the time of N application. The greatest flowering enhancement occurred when N fertilization started 1 week after the first SD when the number of flowering crowns and the number of inflorescences per plant were more than doubled compared with the SD control, while fertilization 2 weeks before SD had no significant effect on these parameters. Importantly, the total number of crowns per plant was not affected by N fertilization at any time, indicating that enhancement of flowering was not due to an increase in potential inflorescence sites. No flowering took place in the control plants in LD. Possible physiological mechanisms involved and practical applications of the findings are discussed.     

Spatial and semantic dimensions of landscape heterogeneity

This paper addresses the challenge of measuring spatial heterogeneity in categorical map data. Spatial heterogeneity is a complex notion that involves both spatial variability and attribute variability, and metrics to capture this are a product of their developers’ simplifying assumptions about both spatial and attribute dimensions. We argue that the predominantly binary treatment of categorical data is frequently an unnecessary oversimplification that can be replaced by ordered measures based on semantic similarity evaluations. We develop a typology of autocorrelation metrics for categorical data that identifies a critical gap: existing measures are limited in their ability to capture variability of both spatial and attribute dimensions simultaneously. We demonstrate an approach to formally characterize the semantic similarity between pairs of categorical data classes as a continuous numeric variable. A series of experiments on synthetic and actual land cover data contrasts the information content provided by metrics representative of all portions of the typology: the recently proposed semantic variogram, the indicator variogram, the contagion index, and the edge contrast index. Experimental results suggest that the typology captures essential qualities of metric information richness. Among our findings is that the commonly used contagion index is directly correlated with Moran’s I for 2-class maps but it fails to distinguish between negatively and positively autocorrelated patterns. We identify the semantic variogram as the only metric that can simultaneously detect both spatial and semantic attribute aspects of categorical autocorrelation. The semantic variogram is also relatively robust to attribute scale changes and therefore less sensitive to class aggregation than the other metrics.     

Enhanced osteoblast differentiation on scaffolds coated with TiO2 compared to SiO2 and CaP coatings

The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replication. NHO were cultured on scaffolds in triplicates. The concentration of cytokines and Ca(2+), and alkaline phosphatase (ALP) activity in the cell media was quantified. The secretion of osteopontin, osteoprotegerin, vascular endothelial growth factor and interleukin-6 was higher from NHO on TiO(2) compared to SiO(2) and CaP. The secretion from cells on the three scaffolds was, however, either similar or lower than the control cells cultured on plastic. The Ca(2+) concentration was higher in cell media on CaP the first week, and no difference in ALP activity was observed. TiO(2) coating induced a higher secretion of factors indicating enhanced osteoblast differentiation as compared to CaP and SiO(2).