Experimental trials on the use of radioimmunoassay for the detection of leptospiral antigens in urine

Trials were conducted on the use of the solid phase radioimmunoassay (RIA) to detect leptospires or their antigens in simulated urine samples. The procedure was relatively simple to perform and appeared to be specific in detecting certain numbers of leptospiral organisms or their antigens in experimentally prepared samples. With this technique, it was possible to examine individual or pooled urine samples for the presence of leptospires within half a day. This technique may be of value for the detection of leptospiruric animals if the sensitivity of the technique could be further increased. Suggestions for the improvement of the procedure are discussed.     

Radiographic and ultrasonographic features of uroliths and other urinary tract filling defects

Radiopaque uroliths and nonradiopaque (water density) uroliths are filling defects encountered in the urinary tracts of dogs and cats. Other free luminal and attached soft tissue density filling defects encountered during uroradiographic special procedures include blood clots, air bubbles, hematomas, granulomas, abscesses, inflammatory and neoplastic polyps. Nonradiopaque uroliths cannot be identified on survey radiographs from other soft tissue dense structures. Gray scale ultrasonography can be used to differentiate nonradiopaque (water dense) uroliths from other soft tissue attached or free luminal filling defects of the excretory pathway. The differential radiographic features of filling defects encountered during cystography and urethrography are described and illustrated.     

Molecular structure of troponin C from chicken skeletal muscle at 3-angstrom resolution

The x-ray structure of chicken skeletal muscle troponin C (TnC), the Ca2+-binding subunit of the troponin complex, shows that the protein is about 70 angstroms long with an unusual dumbbell shape. The carboxyl and amino domains are separated by a single long alpha helix of about nine turns. Only the two high-affinity Ca2+-Mg2+ sites of the COOH-domain are occupied by metal ions resulting in conformational differences between the COOH- and NH2-domains. These differences are probably important in the triggering of muscle contraction by TnC. Also the structure of TnC is relevant in understanding the function of other calcium-regulated proteins, in particular that of calmodulin because of its strong similarity in amino acid sequence.     

Long-term application of animal slurries to grassland alters soil cation balance

Abstract. Soil samples from a 32-year grassland field experiment were taken from 0–5, 5–10, and 10–15 cm soil depths in February 2002. Plots received annual treatments of unamended control, mineral fertilizer, three rates of pig slurry and three rates of cow slurry, each with six replicates. Samples were analysed for cation exchange capacity (CEC), exchangeable cations (Na⁺, K⁺, Ca²⁺, Mg²⁺), pH and Olsen P. Exchangeable sodium percentage (ESP) was calculated as a sodicity indicator. Mean ESP was generally greater for slurry treatments than the control, with a trend of increasing ESP with application rate. This was particularly marked for cow slurry. At 0–5 cm depth ESP increased from 1.18 in the control to 1.75 at the highest rate of pig slurry and 5.60 at the highest rate of cow slurry. Similar trends were shown for CEC, exchangeable Na⁺, K⁺ and Mg²⁺, Ca²⁺ and Olsen P. The build-up of soil P due to slurry applications, together with this combination of physical and chemical factors, may increase the risk of P loss to surface waters, particularly from soils receiving high rates of cow slurry.     

Spatial distribution of Aspergillus flavus and its toxigenic strains on commercial cottonseed from south Texas and its relationship to aflatoxin contamination

The structure of Aspergillus flavus communities associated with south Texas cottonseed was determined by analysing samples from 178 truckloads of commercial cottonseed from 35 gins, extending from Fort Bend County in the north to the Rio Grande Valley in the south, from September 1999 to October 2001. The number of colony-forming units (CFU) of A. flavus on the cottonseed, and the percentage of S strain (%S) were both correlated with aflatoxin contamination of cottonseed. The number of CFU differed between both regions and seasons, while %S differed only between regions. Comparison of maps of CFU and %S revealed that CFU shows a higher variation across years, while %S shows higher spatial variation. The Rio Grande Valley had significantly lower CFU and %S strain than the Coastal Bend and Upper Coast regions. Cottonseed produced in 1999 had significantly more A. flavus than that produced in either 2000 or 2001. Identification of factors dictating geographical variation in S-strain incidence may provide insights that will lead to improved aflatoxin management.     

Effects of Stocking Sac-Fry and Hatchery-Fed Fry on Production of Fingerling Channel Catfish Ictalurus punctatus

Abstract.— Channel catfish Ictalurus punctatus fry are typically held under hatchery conditions for 7 to 14 d after hatching to allow feeding and growth before they are stocked into nursery ponds to produce fingerling catfish. In an attempt to reduce hatchery operating costs, several catfish fingerling producers in Louisiana presently stock fry within 2 d after hatching before yolk absorption is complete. Fry at this stage of development are commonly referred to as "sac-fry." Although research has shown that fry can be stocked at the onset of yolk absorption with no detrimental effects on subsequent fingerling production, stocking sac-fry has been reported to result in reduced fingerling survival. To further investigate this topic, production trials were conducted in experimental outdoor pools over the course of two growing seasons to evaluate the effect of stocking fry of three different ages (2-, 7-, and 14-d post-hatch, DPH) on survival, growth (weight and length), condition factor (K), yield, feed consumption, and feed conversion ratio (FCR) of fingerling catfish. Results from both trials indicated that the age at which fry were stocked had no effect on production characteristics with the exception of growth. Specifically, fingerlings reared from fry stocked at 2 and 7 DPH were significantly larger than fingerlings reared from fry stocked at an age of 14 DPH. These findings suggest that the practice of stocking sac-fry may be a suitable alternative to the traditional procedure of holding and feeding fry under hatchery conditions prior to stocking. However, in order to fully evaluate the effects of early-age stocking of catfish fry on fingerling production, additional studies must be conducted under pond conditions. Furthermore, these studies must be coupled with a rigorous economic analysis before the practice of stocking sac-fry can be recommended to the catfish industry.     

Genetic diversity estimates in Cicer using AFLP analysis

Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic variation among cultivated chickpea and wild Cicer relatives. In total, 214 marker loci were assessed, of which 211 were polymorphic (98.6%) across the 95 accessions that represented 17 species of Cicer. The genetic variation within a species was highest in C. pinnatifidum followed by C. reticulatum and lowest in C. macracanthum. Three main species groups were identified by UPGMA clustering using Nei's pair‐wise distance calculations. Group I included the cultivated species C. arietinum, C. reticulatum and C. echinospermum. Within this group, C. reticulatum accessions were clustered closest to the C. arietinum cultivars ‘Lasseter’, ‘Kaniva’ and ‘Bumper’, supporting the hypothesis that C. reticulatum is the most probable progenitor of the cultivated species. Cicer bijugum, C. judaicum and C. pinnatifidum were clustered together creating group II. Group III contained all nine perennial species assessed and two annual species C. yamashitae and C. cuneatum. The genetic distance detected between group I and group III (0.13) was equivalent to the genetic distance detected between group I and group II (the primary and annual tertiary species, respectively; 0.14). This indicated that the perennial tertiary species may be as valuable for increasing variation to incorporate novel germplasm in the cultigen as the annual tertiary species.     

Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods.

Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.     

MAGNETIC RESONANCE IMAGING FEATURES OF EQUINE NIGROPALLIDAL ENCEPHALOMALACIA

Magnetic resonance imaging (MR) was used to make a diagnosis of equine nigropallidal encephalomalacia in a horse. Equine nigropallidal encephalomalacia is a neurodegenerative disease that has many characteristics with Parkinson-like diseases in humans. Historically, horses were euthanized based on clinical signs and exposure to the toxic weed, yellow star thistle (Centaurea solstitialis). Previously, the disease has only been confirmed on necropsy. MR imaging can provide accurate and sensitive visualization of typical lesions seen in the brain of horses affected with equine nigropallidal encephalomalacia. Lesions were seen on T1-weighted, T2-weighted and proton density images. There was no contrast enhancement following Gd-DTPA administration. Lesions seen on MR were confirmed at necropsy. Using MR to confirm a diagnosis of equine nigropallidal encephalomalacia will prevent unnecessary suffering of horses and expense to owners that would otherwise incur, while further diagnostics are performed.