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排序方式: 共有153条查询结果,搜索用时 15 毫秒
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Miyano H Haritani M Sentsui H Tsuboi T Tanimura N Kimura KM Kobayashi M Obara N Akimoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):457-460
Intranuclear eosinophilic inclusion bodies were seen in the lactiferous duct and sinus epithelium of mammary tissues collected from a cow with clinical mastitis. Transmission electron microscopy revealed herpesvirus particles in these cells. Immunolabeling against anti bovine herpesvirus type 4 (BHV-4) rabbit serum was detected in nuclei that had intranuclear inclusion bodies. In addition, BHV-4 was isolated from the mammary tissue. The viral DNA was detected by nested PCR from the same tissue. This is the first report to describe mammary lesions in association with BHV-4. 相似文献
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C. Vernon Cole Keith Paustian Edward T. Elliott Alister K. Metherell Dennis S. Ojima William J. Parton 《Water, air, and soil pollution》1993,70(1-4):357-371
We present analyses of major driving variable controls on soil C in agroecosystems. Historical changes in soil C storage in agricultural soils are characterized by large losses during transition from natural grasslands and forests. A major driver in more recent times is the steadily increasing rate of net primary production of major land areas in agriculture. Simulation and analytical models are used to predict trajectories and potential soil C storage under possible scenarios of changed management and climate. Database and analytical requirements for extrapolation from regional to global scales are outlined. 相似文献
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Thawatchai Ngamsiri Masamichi Nakajima Srijanya Sukmanomon Naruepon Sukumasavin Wongphatom Kamonrat Uthairat Na-Nakorn Nobuhiko Taniguchi 《Fisheries Science》2007,73(4):792-799
The Mekong giant catfish Pangasianodon gigas is endemic to the Mekong River and is a critically endangered species. The genotypes of the microsatellite DNA (msDNA) and
mitochondrial DNA (mtDNA) markers (right domain of the control region) were detected to evaluate the present status of genetic
divergence of this species from the Mekong River in Thailand and Cambodia. The observed and expected heterozygosity values
of Mekong giant catfish in Thailand and Cambodia were relatively low in comparison with those of other nonendangered freshwater
fish species. These two populations from Thailand and Cambodia showed similar levels of genetic diversity, as evaluated by
the 384 nucleotides of the mtDNA control region with 13 haplotypes. The pairwise F
ST value between the two populations based on the genotype frequencies of msDNA and mtDNA markers suggested a close genetic
relationship between the populations in Thailand and Cambodia. The results of this study support the conclusion that the Mekong
giant catfish is critically endangered. Care should be taken to sustain the genetic diversity of this species, as the level
of genetic variability has already decreased in the wild population. This species is a target species for an ongoing stock
enhancement program in the Mekong River in Thailand. It is proposed to apply these markers for proper broodstock management,
such as for minimal kinship selective breeding in the hatchery. 相似文献
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Kelly Vargas Yoshiho Asakura Minoru Ikeda Nobuhiko Taniguchi Yasuhiro Obata Katsuyuki Hamasaki Kotaro Tsuchiya Shuichi Kitada 《Fisheries Science》2008,74(3):533-543
ABSTRACT: Allozyme variation of the littleneck clam Ruditapes philippinarum was evaluated in four samples from Nameishi and Matsuo in the Ariake Sea, Ryugatake and Ushibuka in the Shiranui Sea off Kyushu Island, Japan, and in one sample from Jinzhou, China, in the Bohai Sea. A Ruditapes bruguieri sample imported from the Korean Bay off Nampo, North Korea was also studied. Among the R. philippinarum samples, heterozygosity varied from 0.265 to 0.301 and F IS estimates indicated significant homozygosity excess in 15 of 40 loci analyzed. Deviations from Hardy–Weinberg equilibrium were significant in all samples ( P < 0.05). Pairwise F ST estimates indicate that genetic differences between the Chinese and Japanese samples were very low, but significantly different from zero. Mixture proportions with 95% confidence intervals of Chinese R. philippinarum in Nameishi and Matsuo were estimated at 0.4098 [0.2512, 0.5705] and 0.4899 [0.3262, 0.6540], respectively. However, genetic invasion of stocked Chinese R. philippinarum into wild populations in the Ariake Sea remains uncertain due to the low precision of the estimates caused by the high similarity of allele frequencies between Jinzhou and the Ariake Sea. 相似文献