全文获取类型
收费全文 | 145篇 |
免费 | 8篇 |
专业分类
林业 | 5篇 |
农学 | 3篇 |
15篇 | |
综合类 | 6篇 |
农作物 | 5篇 |
水产渔业 | 49篇 |
畜牧兽医 | 69篇 |
植物保护 | 1篇 |
出版年
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 7篇 |
2013年 | 5篇 |
2012年 | 11篇 |
2011年 | 7篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 8篇 |
2007年 | 10篇 |
2006年 | 15篇 |
2005年 | 9篇 |
2004年 | 13篇 |
2003年 | 11篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 6篇 |
1996年 | 1篇 |
1993年 | 4篇 |
1992年 | 1篇 |
排序方式: 共有153条查询结果,搜索用时 15 毫秒
111.
Porcine dermatitis and nephropathy syndrome in Japan 总被引:2,自引:0,他引:2
112.
Naito K Maruyama M Dobashi K Tanimura N Kimura K Haritani M Nakajima Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(10):937-939
A holstein calf with congenital chondrodysplastic dwarfism was histopathologically examined. The head of the calf was relatively flat giving a dog-like appearance with its short nose and sloping forehead. Limb bones were dumbbell-like with short diaphysis and hypertrophied metaphyses. Bone marrow was pale, whitish and fatty. In the metaphyseal plates most of chondrocytes were pyknotic with swollen and ghost-like cytoplasm, and were irregularly arranged. Column of calcified cartilage were poorly formed losing comb-like structure. Bone marrow was ischemic with poor hematopoiesis and was moderately replaced by adipose tissue. In articular cartilage, most of chondrocytes were degenerated with ghost-like cytoplasm. Many cartilage canals and occasional bone marrow-like structure were formed. The characteristics lesions of the calf were chondrodysplasia and dyshematopoiesis. 相似文献
113.
Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development 下载免费PDF全文
Hiroki Akizawa Yumi Hoshino Nobuhiko Yamauchi Tomohiro Kono Masashi Takahashi Manabu Kawahara 《Animal Science Journal》2016,87(3):457-461
Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle. 相似文献
114.
Yuji M Fujimoto M Miyata H Inamoto T Qi WM Yamamoto K Warita K Yokoyama T Hoshi N Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):339-346
The possibility of persorption of prefixed bovine serum albumin-coated sheep erythrocytes (BSA-SEs) from mucous epithelial cells and its mechanisms were investigated in rats orally immunized by BSA for 14 consecutive days. On the day after the final oral immunization, the rats were duodenally perfused by BSA-SEs or non-coated SEs. BSA-SEs were also duodenally perfused in non-immunized rats. Thirty min after perfusion, BSA-SEs were significantly more engulfed by late-apoptotic-stage villous columnar epithelial cells in the orally immunized rats than those in other experiments. The specific antibody (SpAb) was detected on the surfaces of BSA-SEs in rats with oral immunization. In Peyer's patches of all animals, no SEs reached the follicle-associated epithelium, because of the close attachment of follicle-associated intestinal villi and the thick mucous layer. BSA-SEs were more frequently persorbed into portal blood in the orally immunized rats than in other rats. Small numbers of BSA-SEs or SEs were detected in the systemic blood of all animals. BSA-SEs were also histologically found in the blood vessels of the liver, but not in mesenteric lymph nodes. These findings suggest that sensitized antigenic particulates are taken up by late-apoptotic-stage villous columnar epithelial cells in the small intestine and are finally persorbed into the systemic blood circulation. The uptake of antigenic particulates might be mediated by its luminal SpAb. 相似文献
115.
Ei-ichirou TAKAHARA Hideto YUASA Miho NISHIDA Youhei MANTANI Kankanam Gamage Sanath UDAYANGA Wang-Mei QI Takashi TAKEUCHI Toshifumi YOKOYAMA Nobuhiko HOSHI Hiroshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):387-393
A portion of the minute chylomicrons less than 75 nm in diameter are transcytosed from
the extravascular tissue into the subepithelial blood capillaries (sBC) in the villous
apices of the rat jejunum. However, the details of the transportation mechanism have not
been clarified. In this study, the endothelial receptor involved in the transportation of
minute chylomicrons into the sBC’s lumina was immunohistochemically and
histoplanimetrically examined in intestinal villi of the rat jejunum. Immunopositivity for
very low density lipoprotein (VLDL) receptor was detected on the luminal and basal
surfaces of the endothelial cells of sBC in approximately 68% of those apices of jejunal
villi that possessed numerous chylomicrons in the lamina propria, while VLDL receptor was
detected on the endothelial cells of sBC in only approximately 8% of intestinal villi that
possessed few or no chylomicrons in the lamina propria. No immunopositivity for LDL
receptor was detected in the sBC of all intestinal villi. These findings suggest that VLDL
receptor is expressed by the endothelial cells of the sBC in conjunction with the filling
of the lamina propria of jejunal villi with many chylomicrons produced by the villous
columnar epithelial cells and that the VLDL receptor mediates the transportation of minute
chylomicrons, maybe VLDL, into the subepithelial portal blood from the extravascular
tissue of the rat jejunal villi. 相似文献
116.
Tetsushi HIRANO Shogo YANAI Takuya OMOTEHARA Rie HASHIMOTO Yuria UMEMURA Naoto KUBOTA Kiichi MINAMI Daichi NAGAHARA Eiko MATSUO Yoshiko AIHARA Ryota SHINOHARA Tomoyuki FURUYASHIKI Youhei MANTANI Toshifumi YOKOYAMA Hiroshi KITAGAWA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1207-1215
Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to
the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from
abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling
cause of honeybee colony collapse disorder, which is damaging crop production that
requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for
neonicotinoids with lower levels than those of insects; there is thus rising concern about
unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the
effects of neonicotinoids would be enhanced under a chronic stressed condition, which is
known to alter the expression of targets of neonicotinoids, i.e.,
neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice
actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day),
for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous
epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione
peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test,
although the locomotor activities were not affected, the anxiety-like behaviors of the
mice were elevated by both CTD and stress. The present study demonstrates that the
behavioral and reproductive effects of CTD become more serious in combination with
environmental stress, which may reflect our actual situation of multiple exposure. 相似文献
117.
Yuria UMEMURA Ryosuke MIYAMOTO Rie HASHIMOTO Kyoko KINOSHITA Takuya OMOTEHARA Daichi NAGAHARA Tetsushi HIRANO Naoto KUBOTA Kiichi MINAMI Shogo YANAI Natsumi MASUDA Hideto YUASA Youhei MANTANI Eiko MATSUO Toshifumi YOKOYAMA Hiroshi KITAGAWA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1587-1598
Mammalian sexual fate is determined by the presence or absence of sex determining
region of the Y chromosome (Sry) in the “bipotential” gonads.
Recent studies have demonstrated that both male and female sexual development are induced
by distinct and active genetic pathways. Breeding the Y chromosome from Mus m.
domesticus poschiavinus (POS) strains into C57BL/6J (B6J) mice
(B6J-XYPOS) has been shown to induce sex reversal (75%: bilateral ovary, 25%:
true hermaphrodites). However, our B6N-XYPOS mice, which were generated by
backcrossing of B6J-XYPOS on an inbred B6N-XX, develop as males (36%: bilateral
testis with fertility as well as bilateral ovary (34%), and the remainder develop as true
hermaphrodites. Here, we investigated in detail the expressions of essential sex-related
genes and histological features in B6N-XYPOS mice from the fetal period to
adulthood. The onsets of both Sry and SRY-box 9 (Sox9) expressions as determined
spatiotemporally by whole-mount immunohistochemistry in the B6N-XYPOS gonads
occurred 2–3 tail somites later than those in B6N-XYB6 gonads, but earlier than
those in B6J-XYPOS, respectively. It is possible that such a small difference
in timing of the Sry expression underlies testicular development in our
B6N-XYPOS. Our study is the first to histologically show the expression and
ectopic localization of a female-related gene in the XYPOS testes and a
male-related gene in the XYPOS ovaries. The results from these and previous
experiments indicate that the interplay between genome variants, epigenetics and
developmental gene regulation is crucial for testis development. 相似文献
118.
Akiyoshi EGASHIRA Nobuhiko YAMAUCHI Keiko TANAKA Chihiro MINE Hitomi OTSUBO Masao MURAKAMI Md. Rashedul ISLAM Misako OHTSUKA Naomi YOSHIOKA Takashi KURAMOTO 《The Journal of reproduction and development》2015,61(6):595-600
The presence of multinucleated blastomeres (MNBs) in embryos is associated with poor developmental
competence in assisted reproductive technologies. This phenomenon is observed not only in humans but also in
other animal species. The purpose of the present study was to investigate the characteristics of embryos with
MNBs (MNB embryos) that could be utilized in embryo transfer. The developmental rate of MNB embryos to the
blastocyst stage (50.8%) was significantly lower than that of normal embryos (73.3%) (P < 0.05). The
clinical pregnancy rates of fresh embryo transfer (ET) using day 2 or day 3 embryos were significantly lower
in MNB embryos (5.1%) compared with normal embryos (24.0%) (P < 0.05). In the case of frozen-thawed ET
using a single vitrified/warmed blastocyst, however, the clinical pregnancy rate of MNB embryos was close to
that of normal embryos (59.1% vs. 52.8%). Thus, the findings of the present study suggest
that the frozen-thawed ET of MNB embryos might improve the potential for implantation followed by successful
pregnancy. 相似文献
119.
The mid-gut gland of scallop Patinopecten yessoensis has been discarded in scallop processing factories as a fishery waste and various attempts have been made to turn the waste
into valuable resources. In the present study, we tried to use mid-gut gland drips from scallop as a source of β-1,3-glucanase.
The mid-gut gland drips were collected in a local fishery factory in Yubetsu-cho, Hokkaido Prefecture. β-1,3-Glucanase was
purified from the mid-gut gland drips by ammonium sulfate fractionation followed by successive chromatography on Toyopearl
Phenyl-650M and Toyopearl DEAE-650M. The scallop β-1,3-glucanase, named PyLam38 in the present study, showed a molecular mass
of approximately 38 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis, and hydrolyzed laminarin, a β-1,3-glucan
from Laminaria sp., producing laminaribiose and glucose with an optimal pH and temperature of 6.0 and 45°C, respectively. PyLam38 exhibited
high transglycosylation activity toward various accepter substrates such as monosaccharides, alcohols and xylooligosaccharides.
Thus, PyLam38 was found to be useful for the production of various novel heterooligosaccharides consisting of laminarioligosaccharides
and various accepters. 相似文献
120.
ABSTRACT: In order to assess a daily change of genetic variability during spawning season, hatched larvae of red sea bream sampled on different dates were assayed by polymorphic markers such as microsatellite DNA (msDNA) and mitochondria DNA (mtDNA) control region. Based on the microsatellite loci, the average number of alleles per locus ranged between 13.7 and 18.3. The expected heterozygosities ranged between 0.843 and 0.919. A total of 23 mtDNA haplotypes were detected via digestion of mtDNA D-loop sequences with five endonucleases: Taq I, Alu I, Mbo I, Rsa I and Hinf I. Significant fluctuation of genetic variability during spawning season was detected by both types of DNA markers. It was suggested that the genetic variability was maintained by pooling the seed fish collected on different spawning dates in a hatchery. 相似文献