An updated checklist of plant agrobiodiversity of northern Italy

Genetic Resources and Crop Evolution - Northern Italy is an area particularly suitable for the cultivation of different crops. It used to be characterized by a high agrobiodiversity. However, it is...     

Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter

Optimising assimilate partitioning to spikes is important to increase wheat yield potential. Novel winter wheat lines (HOSUT), ectopically expressing barley sucrose transporter HvSUT1 controlled by the barley Hordein B1 promoter were used to evaluate the potential of improved sucrose uptake capacity on grain yield and quality under field-like conditions. Three independent HOSUT lines were grown over three years in micro-plots. Grain yield per plot was significantly increased by 28%, together with higher protein yield per plot and higher iron and zinc concentration when compared to the non-transformed control wheat Certo. Thousand grain weight was enhanced, indicating that expression of HvSUT1 increases individual grain sink strength. HOSUT grains are larger, display increased grain width and to a lesser extent grain length, indicating transgene effects at a stage when grains grow under filial control. Grain number per spike was decreased by 15% and protein contents by 5%, on average, especially that of glutenins. Overall, despite some compensating effects on grain number per spike and protein contents, HOSUT lines generate a significant yield advantage. The findings can contribute to understanding determinants of grain size and number and its relationship to grain sink strength and might identify limitations of wheat yield potential.     

Comparative evaluation of compost humic acids and their effects on the germination of switchgrass (Panicum vigatum L.)

Purpose

This study aimed to investigate comparatively the main chemical and physico-chemical properties of the humic acid (HA) fraction of three different composts and to evaluate the bioactive effects of these HAs on the germination and early growth of four populations of switchgrass.

Materials and methods

Three compost HAs isolated from a green compost (HA_GC), a mixed compost (HA_MC), and a coffee compost (HA_CC) were characterized for some chemical and physico-chemical properties, such as ash content, elemental composition, total acidity, carboxylic and phenolic OH group contents, E₄/E₆ ratio, Fourier Transform infrared (FT IR), and fluorescence spectroscopies. In subsequent experiments conducted in vitro in a climatic chamber under controlled conditions, the bioactive effects of the three HAs at concentrations of 10, 50, and 200 mg?L^?1 were tested on the germination and early growth of four switchgrass (Panicum virgatum L.) populations, the octaploids Shelter, Shawnee and Dacotah, and the tetraploid Alamo.

Results and discussion

The ash content and the E₄/E₆ ratio were, respectively, much higher or slightly higher for HA_CC than for the other two HAs. HA_MC showed the lowest C and H contents and the highest O content, whereas HA_GC had the highest N content. The total acidity and phenolic OH group content followed the order: HA_MC?>?HA_CC?>?HA_GC. The fluorescence analysis of the three HAs evidenced a common fluorophore unit possibly associated to simple aromatic structures, such as phenolic-like, hydroxy-substituted benzoic and cinnamic acid derivatives. The FT IR spectra of all HA samples indicated the presence of aromatic phenolic structures. Significant beneficial effects were produced by any HA on switchgrass germination and early growth as a function of the population tested and the HA dose.

Conclusions

Results of this study demonstrated that the addition of compost HAs to the germination medium of four switchgrass populations positively influenced the germination process and the growth of primary root and shoot. Significant correlations were found between HA bioactivity and some HA properties. These results suggest a possible use of compost as soil amendment in areas where switchgrass grows naturally or is cultivated.     

Assessment of Eco-Physiological Performance of Quercus ilex L. Leaves in Urban Area by an Integrated Approach

Bioretention cells, also known as raingardens, are increasingly being constructed as a means to collect, infiltrate, and treat stormwater runoff. There are concerns, however, about how stormwater management practices might function in terms of infiltration and pollutant removal as they age. Saturated hydraulic conductivity (K _sat) values were obtained for eight cells in 2006 and again for three of those cells in 2010 using an infiltrometer. A strong positive correlation of mean K _sat with service time was observed (slope = 10.2?±?2.4 cm/h per year, R ² = 0.67). Results from metals analyses of bioretention media cores collected from six bioretention cells showed the expected trend of Cu and Zn enrichment at the surface while Cd was detected only in one out of 72 media samples analyzed. Sorption isotherms from batch testing of field media samples (T = 22.5 °C, pH = 7.2) were used to estimate metal sorption capacities based on representative stormwater Cd and Zn concentrations. Cu was not considered, as very little of the metal is dissolved under these conditions (22.8?±?7.1 %). The mean equilibrium sorption capacities for Cd (10.2?±?3.1 mg/kg) and Zn (294.9?±?14.9 mg/kg) far exceeded observed levels in the bioretention media such that the remaining sorption capacity was ≥83 % for Zn and ≥90 % for Cd for the cells. Overall, the results of this investigation suggest that bioretention cells can provide many years of effective infiltration (>6 years) and metals removal performance (>25 years).     

The diagnostic relevance of NT‐proBNP and proANP 31–67 measurements in staging of myxomatous mitral valve disease in dogs

Background

There is no agreement in current publications regarding the reliability of serum concentrations of natriuretic peptides (NPs) to detect dogs with subclinical myxomatous mitral valve disease (MMVD) and to differentiate between asymptomatic stages.

Objectives

We sought to compare N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) and pro‐atrial natriuretic peptide 31‐67 (proANP) concentrations between various stages of canine MMVD and to investigate the influence of age, weight, and sex.

Methods

In this prospective study, dogs were classified in different disease stages using the modified Canine Heart failure International Expert Forum (CHIEF) system. Serum NP concentrations were compared between groups.

Results

A total of 559 samples from 116 healthy dogs and 236 dogs with MMVD were analyzed. Using cut‐off values (1207 pmol/L for NT‐proBNP, 1578 fmol/mL for proANP), dogs with MMVD with and without congestive heart failure (CHF) could be differentiated with a sensitivity of 83% for both and specificities of 85% and 86%, respectively. Dogs staged in CHIEF B1 and B2 could not be distinguished based on NP concentrations due to wide variation within the groups. Intact females (means 598 pmol/L and 1036 fmol/mL, respectively) had significantly higher values of both NPs than intact males (315 pmol/L and 836 fmol/mL).

Conclusions

NPs in canine MMVD are useful to discriminate between asymptomatic dogs and dogs with CHF. Due to a large overlap of NP‐concentrations between the groups, NPs do not seem to be useful to differentiate between dogs in stages B1 and B2. Interpretation of NT‐proBNP and proANP values should include consideration of sex‐specific differences.     

Knowledge gaps and challenges for conservation of Mediterranean wetlands: Evidence from a comprehensive inventory and literature analysis for Sardinia

1. Wetlands are some of the most important ecosystems on Earth. They play a key role in mitigating climate change-related events and filtering polluted water, and provide habitats for a wide range of species. Despite their importance, and numerous regulations that support their conservation, wetlands continue to be destroyed. Recent reports have indicated a progressive decline in ecological character of remaining wetlands.
2. This research reviews and extends the present status of wetland knowledge in Sardinia, the second largest Mediterranean island. For the first time, Sardinian wetlands were comprehensively mapped using satellite images and field validation. Impacts were also assessed. Trends in literature published about Sardinian wetlands since 1900 were then analysed, mainly according to the location(s) and topic(s) studied.
3. In total, 2,501 Sardinian wetland sites were identified. The most common impacts observed in the field were vegetative degradation and water pollution. Of these wetlands, 2,274 have never been the subject of a research paper. Despite recent increases in publication rates, there was a lack of even basic knowledge about many wetlands, especially smaller ones. Larger wetlands have been studied from a range of viewpoints.
4. In the light of these results, suggestions for improved awareness, effective management and conservation of Mediterranean wetlands were established. Future work should be directed to filling gaps in basic information, and to improvements in research and conservation, which might include multidisciplinary approaches in support of more comprehensive conservation management plans.

     

An outbreak of equine influenza virus in vaccinated horses in Italy is due to an H3N8 strain closely related to recent North American representatives of the Florida sub-lineage

In December 2005, equine influenza virus infection was confirmed as the cause of clinical respiratory disease in vaccinated horses in Apulia, Italy. The infected horses had been vaccinated with a vaccine that contained strains representatives from both the European (A/eq/Suffolk/89) and American (A/eq/Newmarket/1/93) H3N8 influenza virus lineages, and the H7N7 strain A/eq/Praga/56. Genetic characterization of the hemagglutinin (HA) and neuraminidase (NA) genes of the virus from the outbreak, indicated that the isolate (A/eq/Bari/2005) was an H3N8 strain closely related to recent representatives (Kentucky/5/02-like) of the American sub-lineage Florida, that was introduced in Italy through movement of infected horses from a large outbreak described in 2003 in United Kingdom. Strain A/eq/Bari/2005 displayed 9 amino acid changes in the HA1 subunit protein with respect to the reference American strain A/eq/Newmarket/1/93 contained in the vaccine. Four changes were localized in the antigenic regions C-D and likely accounted for the vaccine failure.     

Methicillin resistance of staphylococci isolated from the skin of dogs with pyoderma

OBJECTIVE: To determine the methicillin-resistant profile of staphylococcal isolates from the skin of dogs with pyoderma. ANIMALS: 90 dogs with pyoderma. PROCEDURE: Staphylococci isolated from dogs with pyoderma were tested for susceptibility to methicillin by use of a standard disk diffusion test with oxacillin disks. The DNA extracted from the isolates was tested for the mecA gene that encodes the penicillin-binding protein 2a (PBP2a) by use of a polymerase chain reaction (PCR) assay. The expression of PBP2a was determined with a commercial latex agglutination assay. Species of staphylococcal isolates were identified by use of morphologic, biochemical, and enzymatic tests. RESULTS: Most of the isolated staphylococci were methicillin-susceptible, coagulase-positive Staphylococcus intermedius isolates. Whereas only 2 of 57 S. intermedius isolates were resistant to methicillin, approximately half of the isolates had the mecA gene and produced PBP2a. Staphylococcus schleiferi was the second most common isolate. Widespread resistance to methicillin was found among S. schleiferi isolates. More coagulase-negative S. schleiferi isolates were identified with mecA gene-mediated resistance to methicillin, compared with coagulase-positive S. schleiferi isolates. CONCLUSIONS AND CLINICAL RELEVANCE: The latex agglutination assay for the detection of PBP2a expression coupled with the PCR assay for the mecA gene may provide new information about emerging antimicrobial resistance among staphylococcal isolates.