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Masafumi SHIMIZU Yoshiko NAKAGAWA Yukio SATO Tamotsu FURUMAI Yasuhiro IGARASHI Hiroyasu ONAKA Ryuji YOSHIDA Hitoshi KUNOH 《Journal of General Plant Pathology》2000,66(4):360-366
To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of
rhododendron were surface-sterilized for use as an isolation source. Nine, six and two isolates, with distinguishing characteristics
based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that
various species of actinomycetes grow in the respective organs of this plant as symbionts or parasites. On an agar medium,
only isolate R-5 commonly formed a clear growth-inhibition zone against two major fungal pathogens of rhododendron, Phytophthora cinnamomi and Pestalotiopsis sydowiana, indicating that this isolate can produce antifungal material(s). Acetone extracts of a liquid culture of R-5 had a broad antimicrobial
spectrum against Gram-positive bacteria, yeast and filamentous fungi. Isolate R-5 was identified as a Streptomyces sp. based on morphological, physiological and chemotaxonomical characteristics. The present results indicate that isolate
R-5 is a suitable candidate for the biocontrol of diseases of rhododendron.
Received 25 March 2000/ Accepted in revised form 18 May 2000 相似文献
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Hiroyuki NAKAYAMA Tetsuya TAKAHASHI Kaori NAKAGAWA‐MIZUYACHI Mitsuo KAWASHIMA 《Animal Science Journal》2011,82(5):679-683
The equilibrium dissociation constant (Kd) and the maximum binding capacity (Bmax) of calcitonin (CT) receptor in the plasma membrane of the anterior pituitary in hens were examined by Scatchard analysis of specific binding of 125I‐labeled chicken CT. Values of Kd and Bmax of CT receptor were smaller in laying hens than in non‐laying hens. A decrease in the Kd and Bmax value of CT receptor was observed in the anterior pituitary after the injection of estradiol‐17β and progesterone into nonlaying hens, but not changed after the injection of 5α‐dihydrotestosterone. During an oviposition cycle, the Kd and the Bmax value decreased 3 h before oviposition. In non‐laying hens, neither the Kd nor the Bmax value changed during a full day period. The present study suggests that the CT action on the anterior pituitary may increase 3 h before oviposition by the effect of estradiol‐17β and progesterone in laying hens. 相似文献
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Abstract. A mycobacterial infection of a freshwater prawn, Macrobrachium rosenbergii de Man, is described. The causative organism was isolated and identified as Mycobacterium sp., Runyon Group II. 相似文献
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Aki OHMI Koichi OHNO James K. CHAMBERS Kazuyuki UCHIDA Taisuke NAKAGAWA Hirotaka TOMIYASU Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):167
There has been an increase in the number of Jack Russell Terriers (JRTs) diagnosed with adenomas and adenocarcinomas of the gastrointestinal tract in Japan. This study retrospectively investigated the clinical and histopathological features and prognosis of adenocarcinomas arising in the gastrointestinal tract in JRT dogs. Seven JRTs and 39 dogs of other breeds diagnosed with gastrointestinal adenocarcinoma were included in the study. The most common sites of gastrointestinal adenocarcinoma in JRTs were the pylorus and rectum. On histopathological examination, these adenocarcinomas showed a papillary or tubular growth pattern, and the lesions were confined within the mucosal epithelium and poorly invasive. Among all dogs with gastric adenocarcinoma, the median survival time (MST) for five of the JRTs could not be determined because more than half of the cases remained alive, while the MST for nine non-JRT dogs was 34 days. Among all dogs with adenocarcinoma in the large intestine, the MST for three of the JRTs could not be determined, while the MST for nine non-JRT dogs was 1,973 days. The difference in MST between JRT and non-JRT dogs with gastric adenocarcinoma was significant (P=0.0220). Since gastrointestinal adenocarcinomas in JRTs show distinct characteristics with respect to their clinical features, treatment course, and prognosis, a different surgical and medical treatment plan should be considered compared to the management of gastrointestinal adenocarcinomas in other dog breeds. 相似文献
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Kosei SAKAI James Ken CHAMBERS Kazuyuki UCHIDA Takayuki NAKAGAWA Ryohei NISHIMURA Tomohiro YONEZAWA Shingo MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):370
Copy number gain (CNG) and/or protein overexpression of ErbB2 have been observed in human breast cancer patients and are associated with poor prognosis. Similarly, ErbB2 overexpression has also been observed in canine mammary carcinoma; however, data on ErbB2 copy number is limited. The purposes of this study were to evaluate ErbB2 copy number in dogs with mammary carcinoma and to investigate associations of ErbB2 CNG with ErbB2 expression, histological and clinical characteristics, and survival. DNA samples were isolated from 59 formalin-fixed paraffin-embedded canine mammary gland tissues (34 carcinoma, 14 adenoma, and 11 normal). Using a digital PCR assay, the ErbB2 copy number in these samples was determined as compared to a reference gene on canine chromosome 8. ErbB2 CNG was detected in 14/34 (41%) carcinomas and 2/14 (14%) adenomas. ErbB2 overexpression was observed in 3/34 (9%) carcinomas but not in adenomas. Neither ErbB2 CNG nor ErbB2 overexpression were detected in the normal controls. There was no significant association of the ErbB2 CNG with histological and clinical characteristics such as age, neutered status, histological grade, tumor size, lymph node involvement, distant metastasis, and clinical stage in the dogs with mammary carcinoma. The presence of ErbB2 CNG, but not ErbB2 overexpression, was significantly related to the shorter overall survival. These findings suggest that ErbB2 CNG is a prognostic factor in dogs with mammary carcinoma. 相似文献