大黄鱼冷诱导结合蛋白(CIRP)基因cDNA克隆及低温胁迫对其时空表达的影响

为研究冷诱导结合蛋白(CIRP)在大黄鱼低温适应过程中的作用,采用同源克隆技术获得了大黄鱼CIRP基因,并通过实时荧光定量PCR(qRT-PCR)技术检测了低温胁迫下大黄鱼各组织中CIRP基因的表达变化。结果显示,大黄鱼CIRP基因cDNA序列全长1211 bp,推测编码一个由182个氨基酸组成、分子量为19 ku的蛋白。序列比对显示硬骨鱼类CIRP基因序列较为保守,特别是N端的RNA识别基序(RRM)高度保守。系统进化树分析显示大黄鱼CIRP与银鳕相似性最高(81.5%),所有硬骨鱼类聚为一个大支。慢性低温胁迫中(12℃缓降至6℃),皮肤和肌肉中CIRP基因的表达量呈随温度降低而升高的趋势,6℃时表达量分别为12℃时的11.56倍和9.03倍;鳃、脑和心脏中CIRP基因表达量均呈先显著上升后显著下降的趋势,其峰值均出现在8℃时,表达量分别为12℃时的5.07、7.69和2.83倍;肠、肾脏、肝脏和脾脏中的表达量随温度降低而下降,6℃时的表达量最低,与12℃时相比降幅分别为80.0%、65.6%、91.2%、55.6%。急性低温胁迫(由12℃骤降至8℃并胁迫4 h)条件下,鳃、皮肤、脑、肝脏、心脏中CIRP基因表达量均呈先升高后降低的变化,反映了机体对低温胁迫的应激、适应过程;肌肉和肠中的表达量则始终显著低于胁迫前。慢性和急性低温胁迫中各组织CIRP表达量变化的差异提示,大黄鱼机体在低温胁迫响应和适应中有着多种调节机制。研究表明,CIRP基因参与了大黄鱼应对低温胁迫的过程。     

番茄植株中几种化学成分的化感效应

从番茄植物的几种有机成分中,选出了５种成分进行单一和２种成分混合的生物活性测定试验。它们分别为：Ａ．邻苯二甲酸二异辛酯,Ｂ．邻苯二甲酸二丁酯,Ｃ．水杨酸甲酯,Ｄ．水杨酸,Ｅ．鞣酸。选用生菜、白菜和萝卜作为受体。结果表明,Ｄ、Ｅ的抑制作用的临界浓度为５×１０＾－４ｍｏｌ／Ｌ,Ａ、Ｂ、Ｃ的抑制作用的临界浓度为２．５×１０＾－２ｍｏｌ／Ｌ;酸与酯之间存在不同程度的协同作用或拮抗作用;混合物的组分、组分物     

森林景观信息网络化简介及其应用

介绍应用国际互联网传播森林景观信息和网上旅游服务概况,论述了森林景观数字信息的几种计算机实现方法,并展望森林景观信息网络化前景。     

红子果实发育和冷冻贮藏果的解剖研究

红子果实的肉质果壁是由托附杯借细胞体积增大发育而来。成熟果壁细胞不含有淀粉粒，但具有大量单宁细胞。速冻果果壁细胞间隙和细胞内有许多小冰晶体，细胞受损害小。缓冻果的胞间冰晶体大而数量少，细胞受损程度大。     

法氏囊超滤液提高仔猪日增重作用的研究

用法氏囊超滤液 (相对分子质量为 1 0 0 0以下 )以不同剂量分 2次间隔 2周免疫断乳仔猪 ,比较各试验组与对照组在日增重、料重比方面的差异。结果表明 :免疫组猪的饲料转化率、日增重显著高于对照组。此外 ,低剂量组 (1mg/头 ,2 5mg/头 )的日增重明显高于高剂量组(5mg/头 ,2 5mg/头 ) ,但料重比则明显低于高剂量组。免疫剂量为 2 5mg/头时 ,促进仔猪生长发育的效果最明显。     

灰色理论在魔芋种质资源创新评价及育种中的应用

应用灰色理论对5份魔芋种质资源的6个主要农艺性状进行分析,评价创新种质的综合表现及各主要农艺性状的相互关系,以期为魔芋新品种选育提供科学依据。关联分析结果表明展开度、株高、叶片长是影响产量的主要因子。     

预热段蚕茧感温与干燥工艺关系的研究

研究表明 ,鲜蛹与半干蛹感温变化趋势相似 ;快速加温与中、慢速加温区间蛹体感温存在极显著差异 ;预热段加温速度以中速偏快 (1 0～ 1 2℃ /min)为宜。茧层潮湿可以使半干蛹感温滞后 2～ 6min ,并且快、中速加温区同工艺对应潮、燥茧层间 ,半干蛹感温差异水平显著。说明茧层水分一方面吸收了空气热量 ,另一方面又阻碍了热能向茧内传递     

A rapid biosensor-based method for quantification of free and glucose-conjugated salicylic acid

Background  

Salicylic acid (SA) is an important signalling molecule in plant defenses against biotrophic pathogens. It is also involved in several other processes such as heat production, flowering, and germination. SA exists in the plant as free SA and as an inert glucose conjugate (salicylic acid 2-O-β-D-glucoside or SAG). Recently, Huang et al. developed a bacterial biosensor that responds to free SA but not SAG, designated as Acinetobacter sp. ADPWH_lux. In this paper we describe an improved methodology for Acinetobacter sp. ADPWH_lux-based free SA quantification, enabling high-throughput analysis, and present an approach for the quantification of SAG from crude plant extracts.     

Effects of enrofloxacin on antioxidant system,microsomal enzymatic activity,and proteomics in porcine liver

Enrofloxacin (EF ) is a widely used fluoroquinolone, usually regarded as a safe and effective treatment for bacterial infections. Adverse effects of EF have previously been demonstrated in some species, but so far there have been no studies looking specifically at the impact of EF on pigs. In this study, three different doses of EF (5, 25 and 125 mg kg bw^?1) were administrated to Bama pigs. The results showed that lipid peroxidation of pig liver tissue occurred with all EF doses. The 125 mg kg dose of EF induced catalase (CAT ) and glutathione peroxidase (GSH ‐px) and increased CYP 450 content in pig liver microsomes. The activity of microsomal NADPH ‐cytochrome C reductase (NCCR ) was elevated at both the 25 and 125 mg kg doses of EF . Microsomal erythromycin N‐demethylase (ERND ) and aminopyrin N‐demethylase (AND ) were inhibited by high doses of EF , while aniline‐4‐hydroxylase (AH ) was unaffected. None of the EF treatments affected superoxide dismutase (SOD ) or cytochrome b5 content. Antioxidases and microsomal enzymes may work together to resist the adverse effects of EF . Proteomic analysis revealed increased protein expression of carboxylesterase (CES ) and alpha‐enolase (ENO 1) in microsomes as a stress response to EF . These results provide new information about the adverse effect of fluoroquinolones and help guide their usage more effectively in the clinic or animal breeding.     

兰州百合枯萎病病原菌鉴定及罹病组织超微结构观察

为明确引起甘肃省兰州百合主产区百合枯萎病的致病镰孢菌种类,对从百合主产区枯萎病罹病植株上分离纯化的4株镰孢菌株进行形态学鉴定、分子生物学鉴定以及致病性测定,同时利用电子显微镜对尖孢镰孢菌Fusarium oxysporum侵入百合鳞片后的细胞超微结构进行观察。结果表明:4株镰孢菌菌株经鉴定分别为尖孢镰孢菌、茄病镰孢菌F. solani、三线镰孢菌F. tricinctum和燕麦镰孢菌F. avenaceum。4株镰孢菌菌株的致病力由强到弱的顺序依次是尖孢镰孢菌、燕麦镰孢菌、茄病镰孢菌、三线镰孢菌;尖孢镰孢菌侵入后,鳞片细胞壁、细胞质膜和细胞核结构被破坏,细胞核附近出现大量线粒体,细胞中淀粉粒数量减少。表明尖孢镰孢菌是兰州百合枯萎病防治的重点防控对象。