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71.
Gang Lu Kan-ichi Sakagami Haruo Tanaka Ryunosuke Hamada 《Soil Science and Plant Nutrition》2013,59(2):147-155
Three Andosols (used as grassland, upland field, and paddy field) and two Gray Lowland soils (used as upland field and paddy field) were examined to study the role of organic matter in the stabilization of soil aggregates. It was found that prolonged use of Andosols as grassland or as upland field enabled to maintain a higher level of soil organic carbon than use as paddy field. However, paddy soil exhibited a greater aggregate stability (determined by wet-sieving procedure) than soil under upland field or grassland management. Comparison between two Gray Lowland soils also showed that aggregates in the paddy soil were more stable than in the upland field soil. Among organic constituents examined (such as sodium pyrophosphate (SPP)-extractable organic matter and carbohydrates), only the contents of hot water (HW)-extractable carbohydrates of the soils were significantly correlated with aggregate stability. Paddy soils which showed a greater aggregate stability contained a larger amount of HW -extractable carbohydrates in the soils, and a larger amount of HW -extractable carbohydrates regardless of the size of their aggregates compared with other soils. These findings suggest that HW -extractable carbohydrates which represents easily decomposable organic materials may be of special importance for the stabilization of aggregates in the soils. 相似文献
72.
Retinoid signaling determines germ cell fate in mice 总被引:1,自引:0,他引:1
Bowles J Knight D Smith C Wilhelm D Richman J Mamiya S Yashiro K Chawengsaksophak K Wilson MJ Rossant J Hamada H Koopman P 《Science (New York, N.Y.)》2006,312(5773):596-600
Germ cells in the mouse embryo can develop as oocytes or spermatogonia, depending on molecular cues that have not been identified. We found that retinoic acid, produced by mesonephroi of both sexes, causes germ cells in the ovary to enter meiosis and initiate oogenesis. Meiosis is retarded in the fetal testis by the action of the retinoid-degrading enzyme CYP26B1, ultimately leading to spermatogenesis. In testes of Cyp26b1-knockout mouse embryos, germ cells enter meiosis precociously, as if in a normal ovary. Thus, precise regulation of retinoid levels during fetal gonad development provides the molecular control mechanism that specifies germ cell fate. 相似文献
73.
Takashi Kimiya Kazuhiro Ohtani Setsuko Satoh Yuko Abe Yoshihiko Ogita Hirohisa Kawakita Hideyuki Hamada Yuko Konishi Satoshi Kubota Akira Tominaga 《Fisheries Science》2008,74(5):1157-1165
Inhibitory effects on degranulation of rat basophilic leukemia (RBL-2H3) cells and mouse eosinophils by marine algae extracts
were examined. More than 50% of the degranulation of RBL-2H3 cells was inhibited by water extracts of Ecklonia cava and Chrysymenia wrightii at a concentration of 100 μg/mL. More than 50% of the degranulation of RBL-2H3 cells was inhibited by methanol extracts of
Petalonia binghamiae, Scytosiphon lomentaria, Undaria pinnatifida, Porphyra dentata, Codium fragile and Ulva japonica at a concentration of 200 μg/mL. Most inhibitory substances in the methanol extracts were partitioned into ethyl acetate
and hexane layers. The ethyl acetate-partitioned layer of methanol extract of Petalonia binghamiae had higher inhibitory effects than the hexane-partitioned layer on the degranulation of RBL-2H3 cells. By contrast, the hexanepartitioned
layer of the same extract had a higher inhibitory effect than the ethyl acetate-partitioned layer on the degranulation of
mouse eosinophils. The ethyl acetate-partitioned layer of methanol extract of Petalonia binghamiae was further separated into eight fractions by silica gel column chromatography. Most inhibited the degranulation of RBL-2H3
cells, but not that of mouse eosinophils significantly. These results suggest methanol extract of Petalonia binghamiae contains materials that inhibit the degranulation of basophils and eosinophils differentially. 相似文献
74.
Brown rice of sugary-1 mutants has a wrinkled character because of the presence of phytoglycogen instead of starch in the inner part of the endosperm. Because the wrinkled phenotype was used as a sole selection marker for progeny of the sugary-1 strain, identification of mutant seeds with improved appearance is very difficult. We found that sugary-1 varieties contained not only phytoglycogen but also free glucose in the endosperm, and these were positively correlated. In the segregated F2 seeds that resulted from crossing Hokurikutou237 (sugary-1) and Koshihikari strains, glucose and phytoglycogen were also significantly correlated. Thus, we identified new sugary types with improved appearance from these progeny using glucose measurements. The F4 seeds of the improved strain had moderate phytoglycogen contents and seed germination characteristics. Native-PAGE showed that pullulanase activity in the improved strain increased in developing seeds compared with Hokurikutou237, although isoamylase activity was extremely low and similar to that in sugary-1 types. The new selection method in this study efficiently aids the development of improved sugary rice types that lack the wrinkled phenotype. 相似文献
75.
A major esterase (designated OsEST1) showing high activity using 1-naphthyl acetate as a substrate was identified from rice bran and purified approximately 239-fold to near-homogeniety. The purified enzyme migrated as a single polypeptide band on native and SDS-polyacrylamide gels and had a molecular mass of 25 kDa under denaturing conditions. Analysis of its tryptic peptides by MALDI-TOF-MS and subsequent data mining identified a corresponding cDNA OsEST1 consisting of 714 nucleotides and encoding a 238 amino acid protein. Analysis of its primary sequence indicated that OsEST1 is a GDSL-motif carboxylester hydrolase belonging to the SGNH protein subfamily in containing the putative catalytic triad of Ser11, Asp187, and His190. OsEST1 showed the highest catalytic activity at approximately pH 8.0–8.5 and at 45 °C with Km and Vmax values for 1-naphthyl acetate of 172 μM and 63.7 μmol/min/mg protein, respectively. However, OsEST1 showed no activity with triacylglycerol. Alignment of the primary sequence of OsEST1 and other rice GDSL-motif esterases/lipases showed that OsEST1 aligns with a specific family of plant SGNH esterases involved in response to dehydration and cuticle formation. These results suggest that OsEST1 is not a lipase but an esterase activity which has some other function in rice, especially during seed development. 相似文献
76.
Hirofumi Arai Wataru Tani Akira Okamoto Kenji Fukunaga Yuki Hamada Katsuyasu Tachibana 《Fisheries Science》2009,75(2):499-505
The commercial value of yellowtail Seriola quinqueradiata meat is often reduced by color deterioration of the dark muscle during storage. The objective of this study was to investigate
the effect of simultaneous dietary supplementation of vitamin C (VC) and vitamin E (VE) to cultured yellowtail on color degradation
of the dark muscle during storage. Yellowtail were fed for 6 or 11 days on a diet containing 1% VC and 1% VE (vitamin group)
or without these vitamins (control group). The amounts of VC and VE in the dark muscle of the vitamin group tended to be higher
than those of the control group. During the storage of sliced meat at 23 and 4°C, the decreases in a* value (redness) of the dark muscle of the vitamin group was slower than those of the control group. The formation of metmyoglobin,
which can occur along with myoglobin oxidation, in the dark muscle of the vitamin group was slower than that of the control
group. These results suggest that simultaneous feeding of high amounts of VC and VE for a short period to yellowtail before
harvesting could suppress the color deterioration of the dark muscle during storage. 相似文献
77.
Galal Khamis AbdElgawad Hamada Frank Schaarschmidt Gerrit T. S. Beemster Han Asard Jutta Papenbrock 《Journal of Agronomy and Crop Science》2019,205(5):490-507
Balanites aegyptiaca is a drought‐tolerant tree naturally distributed in Africa and has a high potential for biofuel production and livelihood. To understand the plant tolerance to drought stress, B. aegyptiaca plants collected from five provenances were subjected for 4 weeks to drought stress through different regimes of soil volumetric water content (VWC, i.e. 25% control, 15% as moderate and 5% as a severe drought stress) followed by 2‐week recovery. Morpho‐physiological responses as well as the changes in antioxidant defences under water stress and recovery were investigated. Drought stress significantly reduced plant biomass‐related parameters, stomatal conductance, quantum efficiency and increased leaf temperature. Each provenance showed specific patterns of stress response reactions that were detected in a cluster analysis. The large leaf area and a high level of lipid peroxidation in Cairo provenance increased its sensitivity to severe drought. For provenances El‐Kharga and Yemen, the highest tocopherol contents and the highest catalytic activities of ascorbate peroxidase (SOD), catalase (CAT), glutathione reductase (GR) and dehydroascorbate reductase (DHAR) were recorded. These traits contributed to the high drought tolerance of these two provenances in comparison with the other provenances. All plants recovered from stress and showed specifically increased activity of glutathione‐S‐transferase (GST) as a repair mechanism. Results showed that the drought tolerance level in B. aegyptiaca is provenance‐dependent. 相似文献
78.
Effects of anthropogenic silt on aquatic macroinvertebrates and abiotic variables in streams in the Brazilian Amazon 总被引:2,自引:0,他引:2
Sheyla Regina Marques Couceiro Neusa Hamada Bruce Rider Forsberg Claudia Padovesi-Fonseca 《Journal of Soils and Sediments》2010,10(1):89-103
Purpose
While environmental risks associated with petroleum extraction such as oil spills or leaks are relatively well known, little attention has been given to the impacts of silt. The increase in petroleum exploitation in Amazonia has resulted in sediment input to aquatic systems, with impacts on their biodiversity. Here we use a combination of field measurements and statistical analyses to evaluate the impacts of anthropogenic silt derived from the construction of roads, borrow pits, and wells during the terrestrial development of gas and oil, on macroinvertebrate communities in streams of the Urucu Petroleum Province in the Central Brazilian Amazon. 相似文献79.
80.