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31.
Hübschle OJ Tjipura-Zaire G Abusugra I di Francesca G Mettler F Pini A Morein B 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):298-303
The experimental field trial with an immunostimulating complex (ISCOM) vaccine has been an occasion to explore the role of a Th1 response in the pathogenesis caused by Mycoplasma mycoides subsp. mycoides small colony (MmmSC) and in immune protection. The ISCOM complex is known to promote Th1 response. Antibodies to MmmSC were detected by indirect enzyme-linked immunosorbent assay (ELISA) in the vaccinated cattle, although the levels were lower than in a previous study. No antibodies were detected by complement fixation test (CF). After the challenge infection, vaccinated animals developed CF antibody response. They showed significantly reduced mortality compared with controls. However, gross pathological and histopathological score for vaccinated animals was as high as for the non-vaccinated, characterized by a high inflammatory reaction with histopathology dominated by interlobular pneumonia with vasculitis. 相似文献
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Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome 总被引:6,自引:0,他引:6
Rodríguez-Arrioja GM Segalés J Calsamiglia M Resendes AR Balasch M Plana-Duran J Casal J Domingo M 《American journal of veterinary research》2002,63(3):354-357
OBJECTIVE: To determine the pattern of infection for porcine circovirus type 2 (PCV2) in a herd of pigs with postweaning multisystemic wasting syndrome (PMWS). ANIMALS: 29 sows and 250 pigs. PROCEDURE: Blood samples were collected from all 3-, 7-, and 12-week old pigs and 59 pigs at 28 weeks of age. Pigs that died during the study were necropsied. Porcine parvovirus and PCV2 antibodies were assayed. A polymerase chain reaction (PCR) was used to detect PCV2 genome in serum of selected pigs. RESULTS: The PMWS started when pigs were 8 weeks old, with a prevalence of 30% in 8- to 10-week-old pigs. Eighty-three pigs died during the period between 3 and 12 weeks of age. Microscopic lesions consistent with PMWS were observed, and PCV2 nucleic acid was detected (50 of 68 pigs). Antibodies to PCV2 decreased from 3 to 7 weeks of age, increased at 12 weeks of age, and were maintained until 28 weeks of age. One sow had a positive result for PCR of serum. Nine, 37 and 8 pigs had PCV2 genome in serum obtained at 7, 12, and 28 weeks of age, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Infection with PCV2 coincided with severe clinical signs; however, infected 28-week-old pigs did not have evidence of disease. Immunity declined over time in young pigs. A long duration of PCV2 viremia was apparent in a high percentage of infected pigs, which may affect transmission and persistence of the virus in a herd. 相似文献
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Poli A Abramo F Barsotti P Leva S Gramiccia M Ludovisi A Mancianti F 《Veterinary parasitology》2002,106(3):181-191
A case of leishmaniosis in domestic cats (Felis catus domesticus) is described. The subject showed a nodular lesion on the eyelid. The diagnosis was achieved by serological, parasitological, and light and electron microscopic investigations. By molecular techniques the aetiological agent was identified as belonging to Leishmania infantum, the species implicated in human and canine leishmaniosis in southern Europe. A preliminary study on the prevalence of asymptomatic feline leishmaniosis, performed in the areas where the infected cat was identified, revealed a low seroprevalence of infection: only 1 (0.9%) of the 110 cat sera examined by indirect fluorescent antibody test was positive for anti-Leishmania antibodies. Because clinical signs in feline leishmaniosis are unspecific and similar to those observed in other diseases commonly found in this species, leishmaniosis must be added to the differential diagnosis by feline veterinary practitioners and adequate serologic and histopathologic investigations must be performed in endemic areas. 相似文献
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The methanol and ethyl acetate extracts of the aerial parts of Cirsium tenoreanum, have been evaluated for their phytochemical constituents and antibacterial and cytotoxic properties. Three flavonoids, apigenin, quercetin-3-O-galactoside and kaempferol-3-O-ramnoside, were isolated. The ethyl acetate extract showed a good antiproliferative activity. 相似文献
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Monica Oreste Giovanni Bubici Michele Poliseno Eustachio Tarasco 《Journal of pest science》2016,89(1):153-160
The virulence of three strains of Beauveria bassiana and one of Metarhizium anisopliae was tested against Trialeurodes vaporariorum and its parasitoid Encarsia formosa in laboratory assays. These strains were previously selected for their virulence against Galleria mellonella and Tenebrio molitor. The commercial B. bassiana strain ATCC 74040, both as pure fungal culture and formulated myco-insecticide (Naturalis), was included in the assays as positive control. First, the entomopathogenic fungal strains were tested for their virulence against T. vaporariorum nymphs on tomato leaf disks. Then, the E. formosa development was evaluated under treatment with the entomopathogenic fungal strains at five different time points from the parasitization of T. vaporariorum nymphs. The virulence of our entomopathogenic fungal strains was superior to that of ATCC 74040, although not significantly, resulting in a cumulative mortality (CM) of T. vaporariorum nymphs 7 days after inoculation (DAI) greater than 86 %. Our M. anisopliae strain CIST8 was the most effective (96.6 % CM 7 DAI), even superior to the myco-insecticide Naturalis (94.2 %), which was more effective than the ATCC 74040 pure strain (85.6 %). The entomopathogenic fungal strains, and especially Naturalis, negatively affected E. formosa development and its parasitization activity of T. vaporariorum nymphs. This effect was more pronounced when the fungal strains were applied before parasitization. Results suggest that the application of entomopathogenic fungi is incompatible with E. formosa release on crops. 相似文献
38.
Jan Čermák Jan Gašpárek Francesca De Lorenzi Hamlyn G. Jones 《Annals of Forest Science》2007,64(5):491-501
The objectives of this paper were (1) to provide general biometry data for an 80-year-old olive (Olea europea L., cv. Coratina) grove in Andria, southern Italy, and (2) to compare different methods for estimating leaf area distributions. Stand biometry was represented by a stocking density of 132 trees ha?1, mean spacing of 8.7 m and mean social area (proportional to spacing and tree size) of about 76 m2 per tree. Trunk total circumference averaged 110 cm and after subtraction of missing or dead parts of stems averaged 81 cm, projected area of crowns averaged 17.7 m2 and the mean tree height was 4.9 m. Leaf distribution was evaluated using calibrated ground-based side photographs through image analysis and through using a simple canopy-layer model (considering hollow volume within tree crowns) and double-Gaussian curves. The mean leaf size was about 5 cm2 (distributed in a log-normal manner over the range of 2 to 12 cm2). Considering whole tree crowns, the mean leaf density was about 2.6 m2m?3; the maximum leaf area occurred in canopy layers between 1.5 to 3 m, tailing with a steeper slope to the crown base and a less steep slope to the tree-top. The foliated volume of olive crowns (mean 33.2 m3) contained on average 145 thousand leaves of the total area of 72.6 m2. The corresponding leaf area index on the stand level (LAI grove = 0.96), was rather low due to low stocking density. However when taking into account only the projected crown areas (and avoiding free space between trees), the mean LAI reached about 3.5 (range from 1–7). The radial pattern of leaf distribution derived from image analysis indicated peak LAI rad values at a distance from the stem of about 60 to 70% of crown radius in trees of different size. The applicability of different approaches to the estimation of the necessary allometric parameters is discussed. 相似文献
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