Isolates of Phytophthora from pepper, produced in Tunisia, were characterised according to molecular and pathogenicity criteria. Polymerase chain reaction amplification of the ITS1 region in the ribosomal DNA resulted in different sized fragments. The pepper isolates and P. nicotianae yielded a fragment of 310bp that distinguished it from P. capsici with a fragment of 270bp. The ribosomal RNA gene amplicons of both internal transcribed spacers and the 5.8 S of the pepper Phytophthora and P. nicotianae were digested with 8 endonucleases. The patterns generated, with the 2 enzymes that cut, were identical for both taxa. This molecular analysis corroborated the morphological and biological characteristics and suggests strongly that the isolates of Phytophthora from pepper belong to the species P. nicotianae. Inoculation of pepper, tomato, eggplant and tobacco plants with the isolates of P. nicotianae from pepper showed they were highly pathogenic on pepper but not on tobacco, while their pathogenicity was weak on tomato and eggplant and was associated with atypical symptoms not observed in the field. These pathogenicity tests suggest that pepper isolates of P. nicotianae are particularly adapted to their host and may thus constitute a forma specialis of P. nicotianae. 相似文献
Flower or fruit thinning is a critical cultural practice in the date palm production chain that affects fruit development, quality and yield and regulates tree yearly bearing. Development of a save and economic thinning agent for date palm is critically required especially under harsh conditions. During 2004 and 2005 seasons, water spray for 3 min was applied at different times following mechanical pollination on ‘Lulu’ date palm cultivar growing under Al-Ain oasis conditions. The results showed that water spray following pollination generally decreased fruit set percentage to different extent depending of the time of application. In this respect, the most effective treatment was water spray after 4 h following pollination. This treatment significantly decreased fruit set percentage (48%) compared to the control (79%), as the mean of both seasons. However, the other treatments including water spray 1 h before pollination also decreased fruit set percentage but to a lesser extent. Fruit quality characteristics especially fruit and flesh weight, length and diameter were significantly increased by water spray treatments during the 2004 season, in contrast to the 2005 season. Thinning with water significantly decreased both bunch weight and total yield per tree at the Tamr stage especially when applied after 4 h following pollination. To the best of our knowledge, this is a pioneer study investigating the role of water as a potential thinning agent for date palm flowers. The use of water spray as a save and economic thinner needs, however, more research work to justify the time following pollination and duration of application which may vary upon cultivar, method of pollination and district. 相似文献
2 Egyptian goats and Boscat rabbits were experimentally inoculated with peste des petits ruminants (PPR) local Egyptian strain (PPR, Egypt 87). The inoculated animals contracted the disease with minor clinical manifestations, accompanied by rise of neutralizing antibodies to PPR virus. Virus was isolated from ocular and nasal secretions, buffy coat, spleen, and liver. No contact infection was observed between inoculated and healthy goats. 相似文献
Veterinary Research Communications - This study evaluated the diuretic, antioxidant, anti-inflammatory, and immunological effects of a commercial diuretic (CD) (composed of ammonium chloride,... 相似文献
Effects of exposing quail eggs to high temperature on the heat tolerance ability and productivity of birds were investigated. Four groups of 600 fertile eggs were randomly selected; the first group was incubated under 37.5 °C and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C (Control). The second group was exposed to 39.1 °C for 2 h/day during 4–14 days of embryogenesis and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C. The third group was incubated under 37.5 °C and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The fourth group was exposed to 39?±?1 °C for 2 h during 4–14 days of embryogenesis and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The temperature applied changed (P?<?0.01) embryo weight and incubation period. Birds exposed to high temperature during brooding had superior growth performance, dressed carcass, body temperature and health traits. Birds subjected to 39?±?1 °C during brooding exhibited decreased feed consumption and body weight gain. Finally, this work suggests that thermal acclimation during embryogenesis might offer a practical method for easing heat stress.
During the last 12 years Arborvitae Leaf Miner (Argyresthia thuiella) was imported from North America to Western- and Central-Europe (1971 the Netherlands, 1975 Federal Republic Germany and 1976 Austria) onThuja occidentalis L. (Cupressacea). In Lower Austria and Vienna there is a tendency rapid to the east, through the dominate westerly winds. Fligh-time took 27 days, moth-slip was 5–10 h in the morning; maximum about 5–6 h. Index of sexus was displated in favour of the males (51.7–66.7%). The moths fed with water-honey showed a longaevitas of 1–15 days maximum about 4 days, and fed with pure water of 8 days. Attack-degree (variational limit) amounted to 2.6–24.5%. The parasitism-role (Necremnus sp., Eulophidae) was between 1.2% and 15.7%. In the laboratory chemical-therapeutics in spray form were without effect. Author's opinion is that this eminent exotic injurious insect must already be calculated as a part of the European fauna. 相似文献
In the present work, for the first time, the chemical components of essential oils (EOs) and extracts from wood branch (WB) resulted from the tree pruning wastes of Schinus molle L. grown in Egypt were evaluated for their antioxidant and antibacterial activities. EOs, methanol (ME), dichloromethane (DCME) and water (WE) extracts as antioxidant and antibacterial activities were measured. Total phenolic and flavonoid contents as well as analysis of extracts by gas chromatography–mass spectrometry (GC–MS) were reported. The major components in EOs were α-elemol, β-pinene, and α-phellandrene, in ME were 6-(4-chlorophenyl)-3-cyano-4-(N-benzylpiperazino)-2H-pyran-2-one, and 2-naphthalene methanol, decahydro-α,α,4a-trimethyl-8-methylene, in DCME were 12-methyl-E,E-3,13-octadecadien-1-ol, and 1,2-benzenedicarboxylic acid, dioctyl ester, and in WE were β-eudesmol, and (Z,Z,Z)-9,12,15-octadecatrienoic acid, 2,3-dihydroxypropyl ester. The highest total antioxidant activity was found with EOs (90 ± 1.23 %) and WE (86.30 ± 1.40 %). The lowest IC50 values of 13.11 ± 3.00, and 12.66 ± 2.15 μg/mL were found with WE and EOs, respectively. EOs and WE were observed to have good antibacterial activity against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Sarcina lutea, Pseudomonas aeruginosa, and Micrococcus luteus. In conclusion, the Schinus molle L. WB EOs and extracts might, indeed, be used as a potential source for pharmaceutical or food industries. 相似文献
The comparative molluscicidal activity of different preparations ofBacillus thuringiensis as Thuricide, SAN 401, SAN 402 and SAN 415, onB. alexandrina snails was studied. The results showed that different preparations ofBac. thuring. have a remarkable molluscicidal activity onB. alexandrina snails. However, the obtained results proved thatBac. thuring. with its preparation SAN 415 was the most effective in killing the snails comparing with the other preparations at the same experimental condition. Prolonged exposure ofB. alexandrina to subletal concentration (250 ppm) of SAN 415 delayed its ovipositary activity. Increasing the dose to 500 ppm resulted in a complete loss of egg production. Comparatively exposure of snail groups to the same doses of Thuricide, SAN 401 and SAN 402 resulted only in decreasing its egg laying capacity. These results proved the high potentially effectiveness ofBac. thuring. in its preparation SAN 415 as a biological control agent forB. alexandrina snails. The wayes by which this biological agent acts on the reproductive system of the snails were discussed. 相似文献