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排序方式: 共有281条查询结果,搜索用时 31 毫秒
81.
Sakaguchi K Iima H Hirayama K Okamoto M Matsuda K Miyasho T Kasamatsu M Hasegawa K Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1217-1220
We report an atypical mycobacterial infection in an Indian flap-shelled turtle, Lissemys punctata punctata, that died in an aquarium in Japan. At necropsy, the turtle showed multiple white nodules on the capsular surface and parenchyma of various organs such as the liver, spleen, intestine, and lung. Histologically, granulomatous inflammation surrounding a central zone of necrosis was observed. Sections stained by the Ziehl-Neelsen method revealed numerous acid-fast bacilli in the cytoplasm of macrophages and in the central area of necrosis. The organisms were identified as a mycobacterial species by PCR and nucleotide sequence analysis and revealed 98-100% homology to M. ulcerans. This is, to our knowledge, the first report of mycobacteriosis due to M. ulcerans in a turtle. 相似文献
82.
Akemi YAMAMOTO Minoru ITOH Yoshikatsu KADOYA Hirokazu KANNO Michi YAMADA Shu FURUYA 《Animal Science Journal》2002,73(4):301-304
An experiment was conducted to determine the effects of supplementing a reduced crude protein (CP) diet with apple pomace on the urinary nitrogen excretion and ammonia emission from slurry in the growing pigs. Four barrows (35 kg bodyweight) were assigned to one of two diets. Each pig was placed in a metabolism cage and fed a low CP, amino acid-supplemented diet (CP 11.33%; low CP diet) or a low CP, amino acid-supplemented diet containing 23.08% dried apple pomace (CP 9.47%; apple pomace diet) for two 14-day experimental periods. After the completion of the first period, the pigs were switched to the other diet. Urine and feces were quantitatively collected daily for 5 days after a 9-day adaptation period. The daily nitrogen intake for the low CP diet and the apple pomace diet was 17.76 g/pig and 18.64 g/pig, respectively. The pigs fed the apple pomace diet excreted more fecal nitrogen (6.86 g/day) than the pigs fed the low CP diet (3.63 g/day) ( P < 0.001), but urinary nitrogen excretion with the apple pomace diet was 3.11 g/day, which was much lower than that for the low CP diet (5.95 g/day) ( P < 0.001). The daily ammonia emission from the mixture of urine and feces determined by an in vitro method was much lower for pigs fed the apple pomace diet (120 mg) than that for pigs fed the low CP diet (603 mg) ( P < 0.01). The addition of apple pomace to a reduced CP, amino acid-supplemented diet reduces urinary nitrogen excretion and thereby ammonia emission. 相似文献
83.
Demonstration of bovine herpesvirus type 1 and Mannheimia haemolytica antigens in specimens stored for up to 22 months in buffered formalin
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Stephen Mutoloki Masahito Ishii Minoru Narita 《Canadian journal of veterinary research》2002,66(1):60-63
Bovine herpesvirus type 1 (BHV-1) and Mannheimia haemolytica antigens were demonstrated in lung tissues that were stored in 10% neutral phosphate buffered formalin for 1 to 22 months using the immunoperoxidase method. There were no differences observed in terms of labelling intensity and distribution of M. haemolytica antigens between specimens stored for 1 and 22 months. The labeling intensity in sections from 2-cm thick specimens was comparable to those from 0.2-cm thick specimens. There was no difference observed between pronase-treated and -untreated sections. However, for BHV-1, the labeling intensity in untreated sections was reduced in tissues that had been stored from 12 to 22 months. Sections from thin specimens stored in neutral buffered formalin for 22 months exhibited a stronger staining intensity than those from thick specimens. 相似文献
84.
Characterization of ammonia-assimilating bacteria in a lagoon for wastewater from a paddock of dairy cattle 总被引:2,自引:0,他引:2
Hiraku SASAKI Gen MARUYAMA Hanatsu SUZUKI Jun NONAKA Masaaki SATO Takako SASAKI Minoru OHTA Yutaka NAKAI 《Animal Science Journal》2002,73(1):73-76
We investigated microorganisms that assimilated ammonia in lagoon treatment processes. Ammonia‐assimilating microorganisms were detected by nitrogen‐limited medium that contained ammonia as the sole nitrogen source. Numbers of ammonia‐assimilating aerobes (log CFU/g) were 3.4, 4.8, 5.0, 4.8 and 5.0 (log CFU/mL) on the culture plate incubated at 4°C, 10°C, 15°C, 20°C and 25°C, respectively. Many isolates used ammonia in high rates when they were purely cultivated in nitrogen‐limited medium added to sterilized lagoon extract. Many of them used ammonia even when they were cultivated in media containing viable microbial flora of the lagoon. Among them, enterobacteriaceae and Pseudomonas sp. were identified by analysis of 16S ribosomal DNA. 相似文献
85.
Shimoyama Y Kirat D Akihara Y Kawasako K Komine M Hirayama K Matsuda K Okamoto M Iwano H Kato S Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):599-604
In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine. 相似文献
86.
Naoaki YAMADA Takashi KITAMORI Fumiyo KITAMORI Kanako ISHIGAMI Koji IWANAGA Taiki ITOU Ryosuke KOBAYASHI Shino KUMABE Takuya DOI Junko SATO Yumi WAKO Minoru TSUCHITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1299-1303
A 7-year-old female boxer dog died suddenly without any clinical signs. It was suspected
that the dog had arrhythmogenic right ventricular cardiomyopathy (ARVC) due to ventricular
premature complexes and ventricular tachycardia at 3 years of age. The final diagnosis of
ARVC was confirmed by histological characteristics, such as loss of cardiocytes and
fibrofatty replacement, occurring in the right and left ventricular walls. In the
cardiocytes, non-lipid vacuoles were observed. Cardiac fibrosis and intimal thickening of
the small arteries occurred without fatty replacement in the inner muscle layer including
the papillary muscles of the left ventricular wall. This paper describes the
pathomorphological details of an ARVC case with coincidental cardiac fibrosis in the inner
muscle layer of the left ventricular wall. 相似文献
87.
Mohamed ELBADAWY Takara SAKIYAMA Rania ABOHATAB Kazuaki SASAKI Minoru SHIMODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):21-26
In the present study, we examined the oral pharmacokinetics of the acidic drugs,
diclofenac (DF) and sulfamonomethoxine (SMM), which have different physicochemical
properties, in Shiba goats. DF and SMM were intravenously and orally administered to 5
male goats using a crossover design. The Tmax of DF and SMM were reached 1.5
and 5.6 hr after they have been orally administered, respectively, and this was followed
by their slow elimination. The elimination of both drugs was markedly faster after being
intravenously rather than orally administered, which indicated flip-flop phenomena after
the oral administration. The mean absorption times (MATs) of DF and SMM were 6 and 15 hr,
respectively. This slow absorption may have been due to slow gastric emptying in goats.
The large difference observed in MATs between DF and SMM may have been because DF, which
is more lipophilic than SMM, was partly absorbed from the forestomach. Therefore, these
results suggest that the absorption of highly lipophilic drugs from the forestomach may be
markedly high in Shiba goats. In case of drugs whose elimination is quite fast, their
efficacies may appear from the early stage after oral administration even in ruminants,
because elimination rate is the determinant factor of Tmax in flip-flop
phenomena. Such drugs may be used orally even in ruminants. 相似文献
88.
Takeshi YUI Tetsuo OHMACHI Kazuya MATSUDA Minoru OKAMOTO Hiroyuki TANIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):467-473
Chordomas of the tip of the tail in 6 ferrets were examined using histopathological,
histochemical and immunohistochemical procedures. Histopathologically, round neoplastic
cells containing numerous cytoplasmic vacuoles of varying sizes, categorized as
“physaliphorous cells”, were observed in the amorphous eosinophilic or pale basophilic
myxoid stroma. Physaliphorous cells were arranged in lobules and in a “chordoid” or
“cobblestone” manner. The neoplasms were diagnosed as benign chordoma without local
invasion and metastasis. Histochemically, the cytoplasm of small neoplastic cells was
positive for periodic acid-Schiff stain and alcian blue (AB) pH 2.5 and pH 1.0 stains, but
negative for hyaluronidase digestion-AB pH 2.5 stain. All neoplastic cells were strongly
stained with colloidal ion, negative for high iron diamine AB pH 2.5 and toluidine blue pH
2.5 stains, and positive for Mayer’s mucicarmine stain. Immunohistochemistry using
antibodies directed against low-molecular-weight cytokeratins (CK18, CK19 and CK20),
vimentin and mucin core protein (MUC5AC) revealed that neoplastic cells had both
epithelial and mesenchymal elements. The expression of low-molecular-weight cytokeratins
suggests that neoplastic cells acquired the properties of glandular epithelial cells and
produced epithelial mucus. Furthermore, the expression of cytokeratins, vimentin, S100
protein, brachyury and epithelial membrane antigen indicates that the neoplasms were
equivalent to the classic type of human chordoma. Therefore, immunohistochemistry using
these antibodies can be useful for the characterization of ferret chordoma. 相似文献
89.
Mikio Mitsuishi Takafumi Oshikata Shino Kumabe Azusa Kobayashi Koshiro Katoku Takeshi Kanno Masao Hamamura Minoru Tsuchitani 《Journal of toxicologic pathology》2015,28(1):1-9
We reevaluated histological slides of dorsal skin in control animals from past percutaneous dose toxicity studies using dogs, rabbits and rats to provide background data concerning histological changes related to preparation and application procedures and vehicles or embrocations of every variety. Acanthosis, dermal or perifollicular inflammatory cell infiltration in dogs; hyperkeratosis, acanthosis, dermal inflammatory cell infiltration or hemorrhage in rabbits; and acanthosis, dermal inflammatory cell infiltration, crust or foreign body granuloma in rats were present as procedure-related underlying histological changes in the control animals. Four mechanical acts, (1) rubbing with gauze to remove an administered substance for reapplication, (2) use of a taut bandage to avoid slipping from the application site, (3) peeling a patch off as a preparation procedure for reapplication, and (4) clipping or shaving, were considered to cause injury to the skin. The degree of influence of the various application procedures was found to be as follows: sham, lotion < cream < ointment and tape in dogs; untreated control, sham < lotion < tape and poultice in rabbits; and sham, sodium carboxymethylcellulose < olive oil and lotion < ointment and tape in rats. The degree of ointment influence on rabbits is equivocal. 相似文献
90.
Mohamed ELBADAWY Kazuaki SASAKI Yuji MIYAZAKI Mohamed ABOUBAKR Waleed Fathy KHALIL Minoru SHIMODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1331-1334
The pharmacokinetics of acetaminophen was investigated following oral dosing to Shiba
goats in order to evaluate the properties of gastric emptying. Acetaminophen was
intravenously and orally administered at 30 mg/kg body weight to goats using a crossover
design with a 3-week washout period. The stability of acetaminophen in rumen juice was
also assessed. Acetaminophen concentrations were measured by HPLC. Since acetaminophen was
stable in rumen juice for 24 hr, the extremely low bioavailability (16%) was attributed to
its hepatic extensive first-pass effect. The mean absorption time and absorption half-life
were unexpectedly short (4.93 and 3.35 hr, respectively), indicating its marked absorption
from the forestomach, which may have been due to its smaller molecular weight. Therefore,
acetaminophen was considered to be unsuitable for evaluating gastric emptying in Shiba
goats. 相似文献