Effect of olive-derived antioxidants (3,4-dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

The aim of the present study was to evaluate the effect of the addition of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during liquid storage at 5°C and 15°C. Semen was collected, pooled, diluted and then divided into aliquots supplemented with different concentrations (5 μg/ml, 10 μg/ml, 50 μg/ml and 100 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. Sperm motility characteristics were assessed in the different samples at 0, 6, 24, 48, 72 and 96 hr after cooling, and a fertility trial was also conducted. The results showed that the antioxidant addition did not significantly improve total and progressive motility in ovine cooled sperm maintained at 15° or 5°C. However, in samples stored at 5°C, LIN (48, 72, 96 hr), STR (0 hr) and WOB (0, 48, 72, 96 hr) values significantly decreased in comparison with control treatment when high antioxidant concentrations were added (MIX100 or HT100). When samples were maintained at 15°C, MIX50 showed significantly higher VCL values than the control treatment after 6 hr cooling, and MIX100 showed significantly lower VCL values at 96 hr after cooling. According to the artificial insemination trial, no significant differences were observed when antioxidants were added. In conclusion, the use of HT and DHPG showed small impact in sperm motility and fertility was not affected (nor detrimentally nor positively) when insemination was carried out using antioxidant-supplemented liquid sperm.     

同源血浆及血清中蓝舌病病毒抗体ELISA检测结果比较

为简化动物血液样品中蓝舌病病毒（BTV）检测及研究方法,采用2种酶联免疫吸附试验（ELISA）,对采自云南省德宏州芒市一家肉牛养殖场的30对牛同源血浆及血清样品进行BTV总抗体及VP7抗体检测。BTV总抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为100%,ΔPI在±5%以内的样品占87%;VP7抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为93%,ΔOD在±0.05范围内的样品占87%。结果表明：利用ELISA检测动物血样中BTV抗体时,血浆样品可替代血清样品;血浆样品的溶血程度不影响检测结果,但粗制血浆中的非溶解性杂质可能影响检测结果,需要使用离心除杂后的血浆样品。     

Mensuration of the rabbit pituitary gland from computed tomography

The aim of this retrospective reference interval observational study was to determine the mensuration of the pituitary gland (hypophysis cerebri) by analyzing CT studies in rabbits without clinical evidence of pituitary disease or central neurologic signs. Though diseases of the rabbit pituitary gland are uncommon, the pituitary gland is essential in regulation of the rabbit's endocrine system, as in other species. Currently, there are minimal published studies that detail the rabbit head anatomy on cross‐sectional imaging, and even less specifically examining the pituitary gland. The pituitary gland was measured by one observer at a single time point from transverse and reconstructed sagittal CT images in a soft‐tissue algorithm in 62 rabbits for a total of 66 rabbit head CT studies. The rabbits ranged from 0.84 to 14 years in age (mean ± SD: 5.46 ± 3.05 years) and 0.92 to 4.95 kg in weight (2.21 ± 0.83 kg). Linear pituitary measurements were performed using electronic calipers. The mean ± SD pituitary height was 4.22 ± 0.57 mm, width was 4.48 ± 0.71 mm, and length was 6.02 ± 0.70 mm. The pituitary gland height‐to‐brain area ratio was 1.10 ± 0.16 mm^?1, which is much higher than the values reported in normal dogs and cats. The age, weight, and sex of the rabbits were not found to have a significant impact on pituitary gland mensuration. These measurements could be useful as a reference range for future rabbit head CT studies and to rule out pituitary enlargement or disease when evaluating rabbit pituitary glands.     

Distribution of the mutant allele of the DMRT3 gene associated with ambling gaits in Japanese native horse populations

There are currently eight native horse populations in Japan, namely, Hokkaido, Kiso, Noma, Taishu, Misaki, Tokara, Miyako, and Yonaguni horses. Since locomotion traits, including gaitedness, are important for riding and packing horses, the genetic properties associated with these traits could be informative for understanding the characteristics and history of these horses. In this study, we investigated the distribution of the mutant allele of DMRT3 gene (DMRT3:p.Ser301Ter) associated with ambling gaits in the Japanese native horse. We also examined haplotypes of SNPs in the 83‐kb region including DMRT3 gene by genotyping four SNPs in this region. The results revealed the presence of DMRT3:p.Ser301Ter in the Hokkaido and Yonaguni populations at allele frequencies of 0.18 and 0.02, respectively, and the observed haplotype associated with DMRT3:p.Ser301Ter was estimated as the most common haplotype in the horses in the world. Since DMRT3:p.Ser301Ter has been hypothesized to spread across Eurasian continent from Medieval England after 850 to 900 CE, our findings of the presence of DMRT3:p.Ser301Ter with the common haplotype in the Japanese native horses will provide a new insight into the history of the Japanese native horse, such as considerable level of gene flow from Eurasian continent after 850 to 900 CE.     

内蒙古发酵酸乳制品中乳酸菌的分离鉴定

以采集自内蒙古锡林郭勒盟正蓝旗的2份发酵酸乳样品为研究对象,采用传统纯化培养技术、16S rDNA基因序列测定并构建系统发育树的方法,对样品中的乳酸菌进行了分离鉴定,以期解析发酵乳中乳酸菌的多样性,积累食品微生物资源。研究结果显示,共分离15株乳酸菌,被鉴定为3个属9个种,3个属分别为乳酸乳球菌属、明串珠菌属和乳杆菌属,9个种分别为乳酸乳球菌叶蝉亚种（Lactococcus lactis subsp. hordniae,3株）、乳酸乳球菌（Lactococcus lactis,1株）、乳酸乳球菌乳脂亚种（Lactococcus lactis subsp. cremoris,1株）、乳明串珠菌（Leuconostoc lactis,3株）、肠膜明串珠菌肠膜亚种（Leuconostoc mesenteroides subsp. mesenteroides,1株）、肠膜明串珠菌右旋葡聚糖亚种（Leuconostoc mesenteroides subsp. dextranicum,1株）、植物乳杆菌（Lactobacillus plantarum,1株）、副干酪乳杆菌（Lactobacillus paracasei,3株）和干酪乳杆菌（Lactobacillus casei,1株）。综上表明,该研究采集的酸乳样品中乳酸菌资源丰富,是内蒙古传统乳制品优良发酵剂的研发和产业化生产的潜在微生物资源。     

Quantification of dwarfing effect of different rootstocks in ‘Picual’ olive cultivar using UAV-photogrammetry

Precision Agriculture - Hedgerow orchard is an olive growing system where trees are planted at a super high-density higher than 20-fold (i.e., 1200–2500 trees ha?1) compared to the...     

BT型杂交粳稻育性及其三系的若干蛋白质标记

 用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）方法，对粳稻BT 细胞质雄性不育系六千辛A，保持系六千辛B，恢复系六千辛R、77302-1，以及杂交组合六千辛A/77302-1的F1和F2 种子的胚乳贮藏蛋白进行了分析。结果表明，在谷蛋白α3区域，恢复系有两条带α3a 和α3b,而六千辛A和六千辛B只有一条带α3。 F2代具有α3的种子和具有α3a 加α3b的种子1∶1分离。谷蛋白α4带的移动速率，恢复系比六千辛A快。把较快的α4带记为α4f。 F2代具有α4的种子和具有α4 加α4f的种子也是1∶1分离，与配子体不育类型的F2代花粉育性恢复基因分离比一致。系谱分析表明六千辛R中α3a 和α3b来源于IR8。六千辛A比六千辛B容易提取醇溶蛋白。     

渭南市玉米适宜籽粒直收品种筛选试验研究

研究旨在筛选渭南地区适用的机械化籽粒直收玉米新品种。试验共征集比较10个玉米品种的农艺性状、产量指标。结果表明,在“陕单620”、“陕单650”、“迪卡517”玉米品种表现出矮秆、穗位整齐度高、耐密、茎秆强度强、抗倒、成熟期籽粒水分含量低、高产、生育期短等宜机收的特性,建议在关中灌区夏玉米种植区推广。     

黑芝麻数量性状的主成份分析及其应用

对３６个黑芝麻品种的８个数量性状进行了主成份分析，以供试品种的４个标准化主成份值为依据，结合具体育种目标，提出了青麻、武宁黑芝麻等１０个可供选配的黑芝麻亲本品种。