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141.
B L Homer G D Butcher R D Miles A F Rossi 《Journal of veterinary diagnostic investigation》1992,4(4):406-411
A field study was designed to determine the prevalence of subclinical infectious bursal disease (IBD) in broiler chickens from a commercial poultry company. Bursae of Fabricius (BF) from two vaccinated and three nonvaccinated broiler flocks were evaluated histologically, and antibody profiles of these broiler and matched parent breeder flocks were established. Lesions of IBD, including lymphoid necrosis, stromal edema, and infiltrates of heterophils and macrophages, were first detected in BF at 24 days of age in both vaccinated and nonvaccinated chickens. At 41 days, all BF had lesions characteristic of IBD, including severe lymphoid depletion, proliferation of epithelial cells, and mild fibroplasia. Although mean maternal antibody levels (measured by enzyme-linked immunosorbent assay) in broilers were apparently protective through day 12, IBD antibodies decreased to nonprotective levels (below 1,000) by day 16 or 20. Titers began to increase by day 28 or 32 because of field exposure. Sentinel birds, placed with broiler flocks, also developed IBD antibody titers. Broiler breeders had low and nonuniform antibody titers. Prevalence of field IBD exposure was high, and existing vaccination programs were not effective. 相似文献
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Miles ED Xue Y Strickland JR Boling JA Matthews JC 《Journal of agricultural and food chemistry》2011,59(17):9691-9699
Neotyphodium coenophialum-infected tall fescue contains ergopeptines. Except for interactions with biogenic amine receptors (e.g., dopamine type-2 receptor, D2R), little is known about how ergopeptines affect animal metabolism. The effect of ergopeptines on bovine nucleoside transporters (NT) was evaluated using Madin-Darby bovine kidney (MDBK) cells. Equilibrative NT1 (ENT1)-like activity accounted for 94% of total NT activity. Inhibitory competition (IC(50)) experiments found that this activity was inhibited by both bromocriptine (a synthetic model ergopeptine and D2R agonist) and ergovaline (a predominant ergopeptine of tall fescue). Kinetic inhibition analysis indicated that bromocriptine inhibited ENT1-like activity through a competitive and noncompetitive mechanism. Domperidone (a D2R antagonist) inhibited ENT1 activity more in the presence than in the absence of bromocriptine and displayed an IC(50) value lower than that of bromocriptine or ergovaline, suggesting that inhibition was not through D2R-mediated events. These novel mechanistic findings imply that cattle consuming endophyte-infected tall fescue have reduced ENT1 activity and, thus, impaired nucleoside metabolism. 相似文献
144.
Susan L. Roberts Jan W. van Wagtendonk A. Keith Miles Douglas A. Kelt 《Biological conservation》2011,(1):610-619
The spotted owl (Strix occidentalis) is a late-successional forest dependent species that is sensitive to forest management practices throughout its range. An increase in the frequency and spatial extent of stand-replacing fires in western North America has prompted concern for the persistence of spotted owls and other sensitive late-successional forest associated species. However, there is sparse information on the effects of fire on spotted owls to guide conservation policies. In 2004–2005, we surveyed for California spotted owls during the breeding season at 32 random sites (16 burned, 16 unburned) throughout late-successional montane forest in Yosemite National Park, California. Our burned areas burned at all severities, but predominately involved low to moderate fire severity. Based on an information theoretic approach, spotted owl detection and occupancy rates were similar between burned and unburned sites. Nest and roost site occupancy was best explained by a model that combined total tree basal area (positive effect) with cover by coarse woody debris (negative effect). The density estimates of California spotted owl pairs were similar in burned and unburned forests, and the overall mean density estimate for Yosemite was higher than previously reported for montane forests. Our results indicate that low to moderate severity fires, historically common within montane forests of the Sierra Nevada, California, maintain habitat characteristics essential for spotted owl site occupancy. These results suggest that managed fires that emulate the historic fire regime of these forests may maintain spotted owl habitat and protect this species from the effects of future catastrophic fires. 相似文献
145.
One goal of mammalian neurobiology is to understand the generation of neuronal activity in large networks. Conceptual schemes have been based on either the properties of single cells or of individual synapses. For instance, the intrinsic oscillatory properties of individual thalamic neurons are thought to underlie thalamic spindle rhythms. This issue has been pursued with a computer model of the CA3 region of the hippocampus that is based on known cellular and synaptic properties. Over a wide range of parameters, this model generates a rhythmic activity at a frequency faster than the firing of individual cells. During each rhythmic event, a few cells fire while most other cells receive synchronous synaptic inputs. This activity resembles the hippocampal theta rhythm as well as synchronized synaptic events observed in vitro. The amplitude and frequency of this emergent rhythmic activity depend on intrinsic cellular properties and the connectivity and strength of both excitatory and inhibitory synapses. 相似文献
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147.
Centrifugal effects in the avian retina 总被引:2,自引:0,他引:2
F A Miles 《Science (New York, N.Y.)》1970,170(961):992-995
Electrical stimulation of the centrifugal fibers to the avian retina can disturb the balance between the excitatory and inhibitory system within the receptive fields of individual retinal ganglion cells. Although the mechanisms may vary from one unit to another, the effect is always to make them fire more readily and to a wider range of visual inputs. 相似文献
148.
149.
Blencowe M 《Science (New York, N.Y.)》2007,317(5839):762-763
150.